tRNA (guanine(37)-N1)-methyltransferase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Met-10+ like-protein, putative

Species:

Leishmania major

UniProt:

TRM5_LEIMA

TriTrypDb:

LmjF.16.0310 , LMJLV39_160008300 , LMJSD75_160008300

Length:

686

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 6
NetGPI	no	yes: 0, no: 6

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	7
GO:0005737	cytoplasm	2	2
GO:0005759	mitochondrial matrix	5	7
GO:0031974	membrane-enclosed lumen	2	7
GO:0043226	organelle	2	7
GO:0043227	membrane-bounded organelle	3	7
GO:0043229	intracellular organelle	3	7
GO:0043231	intracellular membrane-bounded organelle	4	7
GO:0043233	organelle lumen	3	7
GO:0070013	intracellular organelle lumen	4	7
GO:0110165	cellular anatomical entity	1	7

Expansion

Sequence features

Q4QEY9

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QEY9

Function

Biological processes
Term	Name	Level	Count
GO:0001510	RNA methylation	4	2
GO:0002939	tRNA N1-guanine methylation	6	2
GO:0006139	nucleobase-containing compound metabolic process	3	2
GO:0006396	RNA processing	6	2
GO:0006399	tRNA metabolic process	7	2
GO:0006400	tRNA modification	6	2
GO:0006725	cellular aromatic compound metabolic process	3	2
GO:0006807	nitrogen compound metabolic process	2	2
GO:0008033	tRNA processing	8	2
GO:0008152	metabolic process	1	2
GO:0009451	RNA modification	5	2
GO:0009987	cellular process	1	2
GO:0016070	RNA metabolic process	5	2
GO:0030488	tRNA methylation	5	2
GO:0032259	methylation	2	2
GO:0034470	ncRNA processing	7	2
GO:0034641	cellular nitrogen compound metabolic process	3	2
GO:0034660	ncRNA metabolic process	6	2
GO:0043170	macromolecule metabolic process	3	2
GO:0043412	macromolecule modification	4	2
GO:0043414	macromolecule methylation	3	2
GO:0044237	cellular metabolic process	2	2
GO:0044238	primary metabolic process	2	2
GO:0044260	obsolete cellular macromolecule metabolic process	3	2
GO:0046483	heterocycle metabolic process	3	2
GO:0071704	organic substance metabolic process	2	2
GO:0090304	nucleic acid metabolic process	4	2
GO:1901360	organic cyclic compound metabolic process	3	2

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	7
GO:0008168	methyltransferase activity	4	7
GO:0008173	RNA methyltransferase activity	4	7
GO:0008175	tRNA methyltransferase activity	5	7
GO:0008757	S-adenosylmethionine-dependent methyltransferase activity	5	7
GO:0009019	tRNA (guanine-N1-)-methyltransferase activity	7	7
GO:0016423	tRNA (guanine) methyltransferase activity	6	7
GO:0016740	transferase activity	2	7
GO:0016741	transferase activity, transferring one-carbon groups	3	7
GO:0052906	tRNA (guanine(37)-N(1))-methyltransferase activity	8	7
GO:0140098	catalytic activity, acting on RNA	3	7
GO:0140101	catalytic activity, acting on a tRNA	4	7
GO:0140640	catalytic activity, acting on a nucleic acid	2	7

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	215	219	PF00656	0.784
CLV_C14_Caspase3-7	350	354	PF00656	0.672
CLV_NRD_NRD_1	12	14	PF00675	0.606
CLV_NRD_NRD_1	190	192	PF00675	0.599
CLV_NRD_NRD_1	240	242	PF00675	0.746
CLV_NRD_NRD_1	347	349	PF00675	0.557
CLV_NRD_NRD_1	524	526	PF00675	0.321
CLV_NRD_NRD_1	54	56	PF00675	0.528
CLV_NRD_NRD_1	583	585	PF00675	0.374
CLV_PCSK_KEX2_1	239	241	PF00082	0.668
CLV_PCSK_KEX2_1	346	348	PF00082	0.511
CLV_PCSK_KEX2_1	524	526	PF00082	0.333
CLV_PCSK_KEX2_1	53	55	PF00082	0.483
CLV_PCSK_KEX2_1	583	585	PF00082	0.374
CLV_PCSK_KEX2_1	632	634	PF00082	0.452
CLV_PCSK_PC1ET2_1	239	241	PF00082	0.668
CLV_PCSK_PC1ET2_1	632	634	PF00082	0.452
CLV_PCSK_SKI1_1	336	340	PF00082	0.289
CLV_PCSK_SKI1_1	35	39	PF00082	0.493
CLV_PCSK_SKI1_1	398	402	PF00082	0.416
CLV_PCSK_SKI1_1	497	501	PF00082	0.394
CLV_PCSK_SKI1_1	525	529	PF00082	0.321
CLV_PCSK_SKI1_1	58	62	PF00082	0.590
CLV_PCSK_SKI1_1	584	588	PF00082	0.349
CLV_PCSK_SKI1_1	647	651	PF00082	0.366
DEG_APCC_DBOX_1	496	504	PF00400	0.594
DEG_APCC_DBOX_1	57	65	PF00400	0.540
DEG_APCC_DBOX_1	582	590	PF00400	0.399
DEG_SPOP_SBC_1	116	120	PF00917	0.590
DEG_SPOP_SBC_1	544	548	PF00917	0.580
DOC_CKS1_1	122	127	PF01111	0.753
DOC_CKS1_1	85	90	PF01111	0.519
DOC_CYCLIN_RxL_1	644	652	PF00134	0.348
DOC_MAPK_gen_1	20	29	PF00069	0.605
DOC_MAPK_gen_1	336	345	PF00069	0.538
DOC_MAPK_gen_1	434	442	PF00069	0.455
DOC_MAPK_gen_1	524	531	PF00069	0.533
DOC_MAPK_gen_1	53	63	PF00069	0.543
DOC_MAPK_gen_1	632	639	PF00069	0.428
DOC_MAPK_MEF2A_6	632	639	PF00069	0.428
DOC_MAPK_RevD_3	42	55	PF00069	0.496
DOC_PP1_RVXF_1	415	421	PF00149	0.488
DOC_PP1_SILK_1	44	49	PF00149	0.586
DOC_PP2B_PxIxI_1	531	537	PF00149	0.505
DOC_PP4_FxxP_1	411	414	PF00568	0.521
DOC_PP4_FxxP_1	451	454	PF00568	0.521
DOC_PP4_FxxP_1	639	642	PF00568	0.410
DOC_USP7_MATH_1	116	120	PF00917	0.672
DOC_USP7_MATH_1	330	334	PF00917	0.521
DOC_USP7_MATH_1	368	372	PF00917	0.579
DOC_USP7_MATH_1	512	516	PF00917	0.508
DOC_USP7_MATH_1	544	548	PF00917	0.521
DOC_USP7_MATH_1	65	69	PF00917	0.606
DOC_WW_Pin1_4	118	123	PF00397	0.779
DOC_WW_Pin1_4	159	164	PF00397	0.504
DOC_WW_Pin1_4	250	255	PF00397	0.447
DOC_WW_Pin1_4	383	388	PF00397	0.503
DOC_WW_Pin1_4	84	89	PF00397	0.661
LIG_14-3-3_CanoR_1	22	28	PF00244	0.580
LIG_14-3-3_CanoR_1	240	245	PF00244	0.565
LIG_14-3-3_CanoR_1	35	44	PF00244	0.370
LIG_14-3-3_CanoR_1	423	427	PF00244	0.516
LIG_14-3-3_CanoR_1	524	530	PF00244	0.521
LIG_14-3-3_CanoR_1	53	58	PF00244	0.368
LIG_14-3-3_CanoR_1	556	565	PF00244	0.500
LIG_14-3-3_CanoR_1	583	587	PF00244	0.456
LIG_Actin_WH2_2	133	148	PF00022	0.491
LIG_AP2alpha_1	538	542	PF02296	0.594
LIG_BIR_II_1	1	5	PF00653	0.761
LIG_BRCT_BRCA1_1	331	335	PF00533	0.521
LIG_BRCT_BRCA1_1	501	505	PF00533	0.594
LIG_Clathr_ClatBox_1	671	675	PF01394	0.555
LIG_DLG_GKlike_1	240	248	PF00625	0.583
LIG_EH1_1	263	271	PF00400	0.548
LIG_eIF4E_1	264	270	PF01652	0.416
LIG_FHA_1	175	181	PF00498	0.529
LIG_FHA_1	24	30	PF00498	0.485
LIG_FHA_1	243	249	PF00498	0.544
LIG_FHA_1	318	324	PF00498	0.521
LIG_FHA_1	347	353	PF00498	0.607
LIG_FHA_1	386	392	PF00498	0.554
LIG_FHA_1	399	405	PF00498	0.328
LIG_FHA_1	427	433	PF00498	0.505
LIG_FHA_1	526	532	PF00498	0.521
LIG_FHA_1	598	604	PF00498	0.434
LIG_FHA_1	620	626	PF00498	0.511
LIG_FHA_2	127	133	PF00498	0.548
LIG_FHA_2	263	269	PF00498	0.444
LIG_FHA_2	371	377	PF00498	0.483
LIG_FHA_2	668	674	PF00498	0.739
LIG_FHA_2	85	91	PF00498	0.521
LIG_LIR_Apic_2	410	414	PF02991	0.538
LIG_LIR_Apic_2	638	642	PF02991	0.411
LIG_LIR_Apic_2	84	88	PF02991	0.705
LIG_LIR_Gen_1	286	295	PF02991	0.386
LIG_LIR_Gen_1	439	450	PF02991	0.481
LIG_LIR_Gen_1	502	512	PF02991	0.511
LIG_LIR_Gen_1	537	545	PF02991	0.521
LIG_LIR_Gen_1	567	576	PF02991	0.554
LIG_LIR_Nem_3	258	264	PF02991	0.460
LIG_LIR_Nem_3	286	291	PF02991	0.386
LIG_LIR_Nem_3	332	338	PF02991	0.488
LIG_LIR_Nem_3	502	508	PF02991	0.511
LIG_LIR_Nem_3	537	541	PF02991	0.521
LIG_LIR_Nem_3	567	572	PF02991	0.548
LIG_LIR_Nem_3	585	590	PF02991	0.243
LIG_Pex14_2	538	542	PF04695	0.521
LIG_SH2_CRK	34	38	PF00017	0.495
LIG_SH2_CRK	415	419	PF00017	0.521
LIG_SH2_CRK	474	478	PF00017	0.521
LIG_SH2_CRK	612	616	PF00017	0.481
LIG_SH2_GRB2like	419	422	PF00017	0.521
LIG_SH2_NCK_1	389	393	PF00017	0.511
LIG_SH2_NCK_1	415	419	PF00017	0.521
LIG_SH2_NCK_1	612	616	PF00017	0.481
LIG_SH2_SRC	306	309	PF00017	0.594
LIG_SH2_STAP1	306	310	PF00017	0.521
LIG_SH2_STAT5	244	247	PF00017	0.542
LIG_SH2_STAT5	264	267	PF00017	0.451
LIG_SH2_STAT5	590	593	PF00017	0.386
LIG_SH2_STAT5	612	615	PF00017	0.572
LIG_SH2_STAT5	85	88	PF00017	0.736
LIG_SH3_3	119	125	PF00018	0.781
LIG_SH3_3	230	236	PF00018	0.787
LIG_SH3_3	30	36	PF00018	0.445
LIG_SH3_3	538	544	PF00018	0.521
LIG_SUMO_SIM_anti_2	319	325	PF11976	0.521
LIG_SUMO_SIM_par_1	281	286	PF11976	0.506
LIG_SUMO_SIM_par_1	62	68	PF11976	0.608
LIG_SUMO_SIM_par_1	620	627	PF11976	0.490
LIG_SUMO_SIM_par_1	669	675	PF11976	0.555
LIG_TRFH_1	612	616	PF08558	0.481
LIG_TRFH_1	639	643	PF08558	0.525
LIG_TYR_ITIM	304	309	PF00017	0.386
LIG_TYR_ITIM	32	37	PF00017	0.453
LIG_TYR_ITIM	413	418	PF00017	0.386
LIG_UBA3_1	43	50	PF00899	0.575
MOD_CDK_SPK_2	250	255	PF00069	0.443
MOD_CK1_1	112	118	PF00069	0.727
MOD_CK1_1	120	126	PF00069	0.735
MOD_CK1_1	174	180	PF00069	0.595
MOD_CK1_1	209	215	PF00069	0.799
MOD_CK1_1	422	428	PF00069	0.386
MOD_CK1_1	76	82	PF00069	0.724
MOD_CK1_1	99	105	PF00069	0.647
MOD_CK2_1	126	132	PF00069	0.565
MOD_CK2_1	15	21	PF00069	0.531
MOD_CK2_1	209	215	PF00069	0.806
MOD_CK2_1	22	28	PF00069	0.374
MOD_CK2_1	262	268	PF00069	0.395
MOD_CK2_1	283	289	PF00069	0.488
MOD_CK2_1	370	376	PF00069	0.496
MOD_CK2_1	387	393	PF00069	0.498
MOD_CK2_1	5	11	PF00069	0.757
MOD_CK2_1	590	596	PF00069	0.438
MOD_CK2_1	667	673	PF00069	0.709
MOD_CK2_1	84	90	PF00069	0.509
MOD_Cter_Amidation	522	525	PF01082	0.386
MOD_GlcNHglycan	1	4	PF01048	0.758
MOD_GlcNHglycan	101	104	PF01048	0.670
MOD_GlcNHglycan	155	158	PF01048	0.526
MOD_GlcNHglycan	285	288	PF01048	0.489
MOD_GlcNHglycan	299	302	PF01048	0.386
MOD_GlcNHglycan	39	42	PF01048	0.442
MOD_GlcNHglycan	559	562	PF01048	0.614
MOD_GlcNHglycan	592	595	PF01048	0.440
MOD_GlcNHglycan	69	72	PF01048	0.703
MOD_GlcNHglycan	75	78	PF01048	0.694
MOD_GSK3_1	112	119	PF00069	0.651
MOD_GSK3_1	207	214	PF00069	0.757
MOD_GSK3_1	264	271	PF00069	0.548
MOD_GSK3_1	383	390	PF00069	0.588
MOD_GSK3_1	422	429	PF00069	0.364
MOD_GSK3_1	499	506	PF00069	0.255
MOD_GSK3_1	5	12	PF00069	0.757
MOD_N-GLC_1	23	28	PF02516	0.521
MOD_N-GLC_1	297	302	PF02516	0.328
MOD_N-GLC_1	317	322	PF02516	0.158
MOD_N-GLC_1	557	562	PF02516	0.607
MOD_N-GLC_1	99	104	PF02516	0.638
MOD_NEK2_1	37	42	PF00069	0.455
MOD_NEK2_1	400	405	PF00069	0.367
MOD_NEK2_1	426	431	PF00069	0.411
MOD_NEK2_1	499	504	PF00069	0.386
MOD_NEK2_1	545	550	PF00069	0.644
MOD_NEK2_1	610	615	PF00069	0.570
MOD_NEK2_1	635	640	PF00069	0.463
MOD_NEK2_1	649	654	PF00069	0.444
MOD_NEK2_2	330	335	PF00069	0.386
MOD_PK_1	348	354	PF00069	0.491
MOD_PK_1	53	59	PF00069	0.520
MOD_PKA_1	240	246	PF00069	0.585
MOD_PKA_1	346	352	PF00069	0.488
MOD_PKA_1	53	59	PF00069	0.520
MOD_PKA_2	109	115	PF00069	0.619
MOD_PKA_2	240	246	PF00069	0.585
MOD_PKA_2	346	352	PF00069	0.488
MOD_PKA_2	422	428	PF00069	0.375
MOD_PKA_2	53	59	PF00069	0.503
MOD_PKA_2	582	588	PF00069	0.451
MOD_PKB_1	20	28	PF00069	0.573
MOD_PKB_1	346	354	PF00069	0.465
MOD_Plk_1	23	29	PF00069	0.525
MOD_Plk_1	248	254	PF00069	0.554
MOD_Plk_1	317	323	PF00069	0.386
MOD_Plk_1	426	432	PF00069	0.364
MOD_Plk_1	512	518	PF00069	0.255
MOD_Plk_1	619	625	PF00069	0.509
MOD_Plk_4	126	132	PF00069	0.630
MOD_Plk_4	140	146	PF00069	0.430
MOD_Plk_4	268	274	PF00069	0.449
MOD_Plk_4	319	325	PF00069	0.488
MOD_Plk_4	330	336	PF00069	0.386
MOD_Plk_4	42	48	PF00069	0.563
MOD_Plk_4	499	505	PF00069	0.386
MOD_Plk_4	619	625	PF00069	0.509
MOD_Plk_4	667	673	PF00069	0.674
MOD_ProDKin_1	118	124	PF00069	0.778
MOD_ProDKin_1	159	165	PF00069	0.490
MOD_ProDKin_1	250	256	PF00069	0.448
MOD_ProDKin_1	383	389	PF00069	0.500
MOD_ProDKin_1	84	90	PF00069	0.670
MOD_SUMO_rev_2	332	340	PF00179	0.386
MOD_SUMO_rev_2	76	81	PF00179	0.647
TRG_DiLeu_BaEn_1	278	283	PF01217	0.602
TRG_DiLeu_BaEn_1	28	33	PF01217	0.550
TRG_DiLeu_BaEn_1	427	432	PF01217	0.386
TRG_DiLeu_BaLyEn_6	395	400	PF01217	0.483
TRG_ENDOCYTIC_2	306	309	PF00928	0.386
TRG_ENDOCYTIC_2	34	37	PF00928	0.463
TRG_ENDOCYTIC_2	415	418	PF00928	0.386
TRG_ENDOCYTIC_2	474	477	PF00928	0.386
TRG_ER_diArg_1	345	348	PF00400	0.609
TRG_ER_diArg_1	434	437	PF00400	0.294
TRG_ER_diArg_1	52	55	PF00400	0.508
TRG_NES_CRM1_1	329	341	PF08389	0.364
TRG_NLS_MonoExtC_3	678	683	PF00514	0.683
TRG_NLS_MonoExtN_4	236	243	PF00514	0.757

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I219	Leptomonas seymouri	53%	100%
A0A3S7WTS8	Leishmania donovani	93%	100%
A4H8F7	Leishmania braziliensis	73%	98%
A4HWT0	Leishmania infantum	92%	100%
A9T6G5	Physcomitrium patens	28%	100%
B7G5J1	Phaeodactylum tricornutum (strain CCAP 1055/1)	25%	100%
E9AQI8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	91%	100%
F4NUJ6	Batrachochytrium dendrobatidis (strain JAM81 / FGSC 10211)	32%	100%
F6VSS6	Xenopus tropicalis	31%	100%
Q4QEY9	Leishmania major	100%	100%