tRNA (guanine(37)-N1)-methyltransferase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Met-10+ like-protein, putative

Species:

Leishmania braziliensis

UniProt:

TRM5_LEIBR

TriTrypDb:

LbrM.16.0320 , LBRM2903_160008800

Length:

698

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 6
NetGPI	no	yes: 0, no: 6

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	7
GO:0005759	mitochondrial matrix	5	7
GO:0031974	membrane-enclosed lumen	2	7
GO:0043226	organelle	2	7
GO:0043227	membrane-bounded organelle	3	7
GO:0043229	intracellular organelle	3	7
GO:0043231	intracellular membrane-bounded organelle	4	7
GO:0043233	organelle lumen	3	7
GO:0070013	intracellular organelle lumen	4	7
GO:0110165	cellular anatomical entity	1	7
GO:0005737	cytoplasm	2	1

Expansion

Sequence features

A4H8F7

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4H8F7

Function

Biological processes
Term	Name	Level	Count
GO:0001510	RNA methylation	4	1
GO:0002939	tRNA N1-guanine methylation	6	1
GO:0006139	nucleobase-containing compound metabolic process	3	1
GO:0006396	RNA processing	6	1
GO:0006399	tRNA metabolic process	7	1
GO:0006400	tRNA modification	6	1
GO:0006725	cellular aromatic compound metabolic process	3	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008033	tRNA processing	8	1
GO:0008152	metabolic process	1	1
GO:0009451	RNA modification	5	1
GO:0009987	cellular process	1	1
GO:0016070	RNA metabolic process	5	1
GO:0030488	tRNA methylation	5	1
GO:0032259	methylation	2	1
GO:0034470	ncRNA processing	7	1
GO:0034641	cellular nitrogen compound metabolic process	3	1
GO:0034660	ncRNA metabolic process	6	1
GO:0043170	macromolecule metabolic process	3	1
GO:0043412	macromolecule modification	4	1
GO:0043414	macromolecule methylation	3	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0044260	obsolete cellular macromolecule metabolic process	3	1
GO:0046483	heterocycle metabolic process	3	1
GO:0071704	organic substance metabolic process	2	1
GO:0090304	nucleic acid metabolic process	4	1
GO:1901360	organic cyclic compound metabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	7
GO:0008168	methyltransferase activity	4	7
GO:0008173	RNA methyltransferase activity	4	7
GO:0008175	tRNA methyltransferase activity	5	7
GO:0008757	S-adenosylmethionine-dependent methyltransferase activity	5	7
GO:0009019	tRNA (guanine-N1-)-methyltransferase activity	7	7
GO:0016423	tRNA (guanine) methyltransferase activity	6	7
GO:0016740	transferase activity	2	7
GO:0016741	transferase activity, transferring one-carbon groups	3	7
GO:0052906	tRNA (guanine(37)-N(1))-methyltransferase activity	8	7
GO:0140098	catalytic activity, acting on RNA	3	7
GO:0140101	catalytic activity, acting on a tRNA	4	7
GO:0140640	catalytic activity, acting on a nucleic acid	2	7

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	215	219	PF00656	0.775
CLV_C14_Caspase3-7	367	371	PF00656	0.467
CLV_NRD_NRD_1	19	21	PF00675	0.416
CLV_NRD_NRD_1	190	192	PF00675	0.617
CLV_NRD_NRD_1	238	240	PF00675	0.734
CLV_NRD_NRD_1	347	349	PF00675	0.552
CLV_NRD_NRD_1	530	532	PF00675	0.293
CLV_PCSK_KEX2_1	21	23	PF00082	0.372
CLV_PCSK_KEX2_1	240	242	PF00082	0.632
CLV_PCSK_KEX2_1	346	348	PF00082	0.518
CLV_PCSK_KEX2_1	530	532	PF00082	0.302
CLV_PCSK_KEX2_1	638	640	PF00082	0.445
CLV_PCSK_KEX2_1	686	688	PF00082	0.607
CLV_PCSK_PC1ET2_1	21	23	PF00082	0.372
CLV_PCSK_PC1ET2_1	240	242	PF00082	0.632
CLV_PCSK_PC1ET2_1	638	640	PF00082	0.445
CLV_PCSK_PC1ET2_1	686	688	PF00082	0.607
CLV_PCSK_SKI1_1	336	340	PF00082	0.275
CLV_PCSK_SKI1_1	397	401	PF00082	0.450
CLV_PCSK_SKI1_1	457	461	PF00082	0.210
CLV_PCSK_SKI1_1	496	500	PF00082	0.390
CLV_PCSK_SKI1_1	531	535	PF00082	0.317
CLV_PCSK_SKI1_1	58	62	PF00082	0.540
CLV_PCSK_SKI1_1	686	690	PF00082	0.667
DEG_APCC_DBOX_1	495	503	PF00400	0.590
DEG_APCC_DBOX_1	57	65	PF00400	0.552
DOC_AGCK_PIF_1	593	598	PF00069	0.261
DOC_CKS1_1	124	129	PF01111	0.650
DOC_CYCLIN_yClb3_PxF_3	124	130	PF00134	0.446
DOC_CYCLIN_yCln2_LP_2	387	393	PF00134	0.359
DOC_MAPK_DCC_7	203	213	PF00069	0.459
DOC_MAPK_gen_1	20	29	PF00069	0.602
DOC_MAPK_gen_1	530	537	PF00069	0.530
DOC_MAPK_gen_1	55	63	PF00069	0.535
DOC_MAPK_gen_1	638	645	PF00069	0.422
DOC_MAPK_MEF2A_6	638	645	PF00069	0.422
DOC_PP1_RVXF_1	414	420	PF00149	0.517
DOC_PP1_SILK_1	44	49	PF00149	0.585
DOC_PP2B_LxvP_1	86	89	PF13499	0.496
DOC_PP4_FxxP_1	410	413	PF00568	0.517
DOC_PP4_FxxP_1	450	453	PF00568	0.517
DOC_PP4_FxxP_1	645	648	PF00568	0.405
DOC_USP7_MATH_1	209	213	PF00917	0.579
DOC_USP7_MATH_1	223	227	PF00917	0.566
DOC_USP7_MATH_1	234	238	PF00917	0.717
DOC_USP7_MATH_1	330	334	PF00917	0.517
DOC_USP7_MATH_1	360	364	PF00917	0.662
DOC_USP7_MATH_1	484	488	PF00917	0.541
DOC_USP7_MATH_1	510	514	PF00917	0.560
DOC_USP7_UBL2_3	456	460	PF12436	0.396
DOC_WW_Pin1_4	120	125	PF00397	0.747
DOC_WW_Pin1_4	159	164	PF00397	0.518
DOC_WW_Pin1_4	204	209	PF00397	0.604
DOC_WW_Pin1_4	232	237	PF00397	0.597
DOC_WW_Pin1_4	386	391	PF00397	0.503
LIG_14-3-3_CanoR_1	114	124	PF00244	0.461
LIG_14-3-3_CanoR_1	22	28	PF00244	0.575
LIG_14-3-3_CanoR_1	262	267	PF00244	0.374
LIG_14-3-3_CanoR_1	422	426	PF00244	0.517
LIG_14-3-3_CanoR_1	530	536	PF00244	0.517
LIG_14-3-3_CanoR_1	55	61	PF00244	0.342
LIG_14-3-3_CanoR_1	95	101	PF00244	0.529
LIG_AP2alpha_1	544	548	PF02296	0.590
LIG_BIR_II_1	1	5	PF00653	0.759
LIG_BRCT_BRCA1_1	331	335	PF00533	0.517
LIG_BRCT_BRCA1_1	500	504	PF00533	0.590
LIG_EH1_1	263	271	PF00400	0.555
LIG_eIF4E_1	264	270	PF01652	0.452
LIG_FHA_1	175	181	PF00498	0.531
LIG_FHA_1	184	190	PF00498	0.472
LIG_FHA_1	24	30	PF00498	0.481
LIG_FHA_1	254	260	PF00498	0.427
LIG_FHA_1	318	324	PF00498	0.512
LIG_FHA_1	398	404	PF00498	0.234
LIG_FHA_1	532	538	PF00498	0.517
LIG_FHA_1	552	558	PF00498	0.469
LIG_FHA_2	141	147	PF00498	0.472
LIG_FHA_2	263	269	PF00498	0.452
LIG_FHA_2	371	377	PF00498	0.433
LIG_FHA_2	666	672	PF00498	0.643
LIG_LIR_Apic_2	149	154	PF02991	0.590
LIG_LIR_Apic_2	644	648	PF02991	0.405
LIG_LIR_Apic_2	84	88	PF02991	0.703
LIG_LIR_Gen_1	286	295	PF02991	0.383
LIG_LIR_Gen_1	438	449	PF02991	0.517
LIG_LIR_Gen_1	487	493	PF02991	0.540
LIG_LIR_Gen_1	501	512	PF02991	0.507
LIG_LIR_Gen_1	543	551	PF02991	0.517
LIG_LIR_Gen_1	573	582	PF02991	0.554
LIG_LIR_Nem_3	258	264	PF02991	0.456
LIG_LIR_Nem_3	286	291	PF02991	0.331
LIG_LIR_Nem_3	332	338	PF02991	0.517
LIG_LIR_Nem_3	487	491	PF02991	0.457
LIG_LIR_Nem_3	501	507	PF02991	0.493
LIG_LIR_Nem_3	543	547	PF02991	0.517
LIG_LIR_Nem_3	573	578	PF02991	0.547
LIG_LIR_Nem_3	591	596	PF02991	0.250
LIG_PCNA_yPIPBox_3	262	270	PF02747	0.303
LIG_PCNA_yPIPBox_3	48	61	PF02747	0.335
LIG_Pex14_2	544	548	PF04695	0.517
LIG_SH2_CRK	414	418	PF00017	0.517
LIG_SH2_CRK	473	477	PF00017	0.517
LIG_SH2_CRK	618	622	PF00017	0.468
LIG_SH2_GRB2like	418	421	PF00017	0.517
LIG_SH2_NCK_1	414	418	PF00017	0.517
LIG_SH2_NCK_1	618	622	PF00017	0.468
LIG_SH2_SRC	306	309	PF00017	0.590
LIG_SH2_STAP1	306	310	PF00017	0.517
LIG_SH2_STAP1	596	600	PF00017	0.272
LIG_SH2_STAT5	244	247	PF00017	0.601
LIG_SH2_STAT5	264	267	PF00017	0.459
LIG_SH2_STAT5	618	621	PF00017	0.560
LIG_SH2_STAT5	85	88	PF00017	0.732
LIG_SH3_3	121	127	PF00018	0.728
LIG_SH3_3	227	233	PF00018	0.766
LIG_SH3_3	30	36	PF00018	0.440
LIG_SH3_3	544	550	PF00018	0.517
LIG_SH3_3	61	67	PF00018	0.386
LIG_SUMO_SIM_anti_2	319	325	PF11976	0.517
LIG_SUMO_SIM_anti_2	59	65	PF11976	0.337
LIG_SUMO_SIM_par_1	254	260	PF11976	0.313
LIG_SUMO_SIM_par_1	281	286	PF11976	0.507
LIG_SUMO_SIM_par_1	378	383	PF11976	0.371
LIG_SUMO_SIM_par_1	62	70	PF11976	0.646
LIG_SUMO_SIM_par_1	626	633	PF11976	0.431
LIG_TRAF2_1	373	376	PF00917	0.323
LIG_TRAF2_1	624	627	PF00917	0.313
LIG_TRFH_1	618	622	PF08558	0.468
LIG_TRFH_1	645	649	PF08558	0.519
LIG_TYR_ITIM	304	309	PF00017	0.517
LIG_TYR_ITIM	32	37	PF00017	0.446
LIG_TYR_ITIM	412	417	PF00017	0.517
LIG_UBA3_1	43	50	PF00899	0.574
LIG_WRC_WIRS_1	595	600	PF05994	0.270
MOD_CDK_SPxxK_3	232	239	PF00069	0.519
MOD_CK1_1	123	129	PF00069	0.594
MOD_CK1_1	207	213	PF00069	0.745
MOD_CK1_1	235	241	PF00069	0.507
MOD_CK1_1	386	392	PF00069	0.365
MOD_CK1_1	421	427	PF00069	0.517
MOD_CK1_1	552	558	PF00069	0.498
MOD_CK1_1	76	82	PF00069	0.725
MOD_CK1_1	99	105	PF00069	0.637
MOD_CK2_1	140	146	PF00069	0.500
MOD_CK2_1	209	215	PF00069	0.799
MOD_CK2_1	22	28	PF00069	0.369
MOD_CK2_1	262	268	PF00069	0.463
MOD_CK2_1	283	289	PF00069	0.487
MOD_CK2_1	370	376	PF00069	0.447
MOD_CK2_1	386	392	PF00069	0.606
MOD_CK2_1	510	516	PF00069	0.410
MOD_CK2_1	596	602	PF00069	0.439
MOD_CK2_1	621	627	PF00069	0.324
MOD_CK2_1	665	671	PF00069	0.615
MOD_Cter_Amidation	528	531	PF01082	0.317
MOD_GlcNHglycan	1	4	PF01048	0.761
MOD_GlcNHglycan	285	288	PF01048	0.487
MOD_GlcNHglycan	299	302	PF01048	0.317
MOD_GlcNHglycan	364	367	PF01048	0.648
MOD_GlcNHglycan	38	42	PF01048	0.447
MOD_GlcNHglycan	598	601	PF01048	0.438
MOD_GlcNHglycan	673	676	PF01048	0.514
MOD_GlcNHglycan	75	78	PF01048	0.686
MOD_GSK3_1	115	122	PF00069	0.747
MOD_GSK3_1	155	162	PF00069	0.481
MOD_GSK3_1	207	214	PF00069	0.746
MOD_GSK3_1	253	260	PF00069	0.404
MOD_GSK3_1	264	271	PF00069	0.515
MOD_GSK3_1	382	389	PF00069	0.601
MOD_GSK3_1	549	556	PF00069	0.540
MOD_GSK3_1	560	567	PF00069	0.475
MOD_GSK3_1	665	672	PF00069	0.635
MOD_GSK3_1	95	102	PF00069	0.658
MOD_N-GLC_1	119	124	PF02516	0.735
MOD_N-GLC_1	159	164	PF02516	0.403
MOD_N-GLC_1	262	267	PF02516	0.309
MOD_N-GLC_1	297	302	PF02516	0.317
MOD_N-GLC_1	317	322	PF02516	0.317
MOD_N-GLC_1	360	365	PF02516	0.515
MOD_N-GLC_1	552	557	PF02516	0.640
MOD_N-GLC_1	95	100	PF02516	0.657
MOD_NEK2_1	37	42	PF00069	0.454
MOD_NEK2_1	382	387	PF00069	0.387
MOD_NEK2_1	498	503	PF00069	0.517
MOD_NEK2_1	551	556	PF00069	0.638
MOD_NEK2_1	616	621	PF00069	0.560
MOD_NEK2_1	641	646	PF00069	0.410
MOD_NEK2_1	655	660	PF00069	0.363
MOD_NEK2_2	223	228	PF00069	0.561
MOD_NEK2_2	330	335	PF00069	0.517
MOD_NEK2_2	484	489	PF00069	0.521
MOD_PIKK_1	155	161	PF00454	0.464
MOD_PIKK_1	174	180	PF00454	0.493
MOD_PIKK_1	655	661	PF00454	0.363
MOD_PKA_1	240	246	PF00069	0.568
MOD_PKA_2	110	116	PF00069	0.593
MOD_PKA_2	174	180	PF00069	0.336
MOD_PKA_2	240	246	PF00069	0.517
MOD_PKA_2	3	9	PF00069	0.658
MOD_PKA_2	421	427	PF00069	0.517
MOD_PKA_2	94	100	PF00069	0.564
MOD_PKB_1	20	28	PF00069	0.570
MOD_Plk_1	248	254	PF00069	0.540
MOD_Plk_1	257	263	PF00069	0.376
MOD_Plk_1	317	323	PF00069	0.487
MOD_Plk_1	382	388	PF00069	0.364
MOD_Plk_1	552	558	PF00069	0.562
MOD_Plk_1	6	12	PF00069	0.576
MOD_Plk_4	140	146	PF00069	0.405
MOD_Plk_4	240	246	PF00069	0.418
MOD_Plk_4	268	274	PF00069	0.453
MOD_Plk_4	319	325	PF00069	0.517
MOD_Plk_4	330	336	PF00069	0.469
MOD_Plk_4	42	48	PF00069	0.472
MOD_Plk_4	498	504	PF00069	0.517
MOD_Plk_4	588	594	PF00069	0.403
MOD_ProDKin_1	120	126	PF00069	0.742
MOD_ProDKin_1	159	165	PF00069	0.504
MOD_ProDKin_1	204	210	PF00069	0.621
MOD_ProDKin_1	232	238	PF00069	0.594
MOD_ProDKin_1	386	392	PF00069	0.501
MOD_SUMO_rev_2	332	340	PF00179	0.517
MOD_SUMO_rev_2	370	379	PF00179	0.347
TRG_DiLeu_BaEn_1	278	283	PF01217	0.593
TRG_DiLeu_BaEn_1	28	33	PF01217	0.549
TRG_DiLeu_BaEn_1	426	431	PF01217	0.517
TRG_ENDOCYTIC_2	306	309	PF00928	0.517
TRG_ENDOCYTIC_2	34	37	PF00928	0.454
TRG_ENDOCYTIC_2	414	417	PF00928	0.517
TRG_ENDOCYTIC_2	473	476	PF00928	0.475
TRG_ER_diArg_1	19	22	PF00400	0.371
TRG_ER_diArg_1	346	348	PF00400	0.571
TRG_NLS_MonoExtC_3	685	691	PF00514	0.663
TRG_NLS_MonoExtN_4	236	243	PF00514	0.740
TRG_NLS_MonoExtN_4	683	690	PF00514	0.541

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I219	Leptomonas seymouri	52%	100%
A0A3S7WTS8	Leishmania donovani	73%	100%
A4H8F7	Leishmania braziliensis	100%	100%
A4HWT0	Leishmania infantum	73%	100%
A9T6G5	Physcomitrium patens	29%	100%
E9AQI8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	74%	100%
Q4QEY9	Leishmania major	73%	99%