Phosphatidylinositol:ceramide inositolphosphotransferase

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

sphingomyelin/ceramide phosphorylethanolamine synthase, bifunctional

Species:

Leishmania major

UniProt:

SLS_LEIMA

TriTrypDb:

LmjF.35.4990 , LMJLV39_350057900 , LMJSD75_350057200

Length:

338

Annotations

LeishMANIAdb annotations

Publication identifier(s): 20457606
Bidirectional lipid inositolphosphotransferase (experimentally validated). Homologous to animal phosphatidylcholine:ceramide cholinephosphotransferases. Expanded on the Trypanosoma lineage.

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 15
NetGPI	no	yes: 0, no: 15

Cellular components
Term	Name	Level	Count
GO:0005768	endosome	7	2
GO:0005794	Golgi apparatus	5	2
GO:0005886	plasma membrane	3	2
GO:0016020	membrane	2	16
GO:0020016	ciliary pocket	2	2
GO:0031410	cytoplasmic vesicle	6	2
GO:0031982	vesicle	4	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0097708	intracellular vesicle	5	2
GO:0110165	cellular anatomical entity	1	16

Expansion

Sequence features

E9AFX2

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AFX2

Function

Biological processes
Term	Name	Level	Count
GO:0006066	alcohol metabolic process	3	2
GO:0006576	biogenic amine metabolic process	5	2
GO:0006580	ethanolamine metabolic process	5	2
GO:0006629	lipid metabolic process	3	16
GO:0006643	membrane lipid metabolic process	4	16
GO:0006665	sphingolipid metabolic process	4	16
GO:0006672	ceramide metabolic process	4	2
GO:0006793	phosphorus metabolic process	3	16
GO:0006796	phosphate-containing compound metabolic process	4	16
GO:0006807	nitrogen compound metabolic process	2	16
GO:0008152	metabolic process	1	16
GO:0008610	lipid biosynthetic process	4	2
GO:0009058	biosynthetic process	2	2
GO:0009308	amine metabolic process	4	2
GO:0009309	amine biosynthetic process	5	2
GO:0009987	cellular process	1	16
GO:0016310	phosphorylation	5	16
GO:0030148	sphingolipid biosynthetic process	5	2
GO:0034308	primary alcohol metabolic process	4	2
GO:0034309	primary alcohol biosynthetic process	5	2
GO:0034641	cellular nitrogen compound metabolic process	3	2
GO:0042401	biogenic amine biosynthetic process	6	2
GO:0042439	ethanolamine-containing compound metabolic process	4	2
GO:0043603	amide metabolic process	3	2
GO:0043604	amide biosynthetic process	4	2
GO:0044237	cellular metabolic process	2	16
GO:0044238	primary metabolic process	2	16
GO:0044249	cellular biosynthetic process	3	2
GO:0044255	cellular lipid metabolic process	3	16
GO:0044271	cellular nitrogen compound biosynthetic process	4	2
GO:0044281	small molecule metabolic process	2	2
GO:0044283	small molecule biosynthetic process	3	2
GO:0046165	alcohol biosynthetic process	4	2
GO:0046335	ethanolamine biosynthetic process	6	2
GO:0046467	membrane lipid biosynthetic process	4	2
GO:0046513	ceramide biosynthetic process	5	2
GO:0071704	organic substance metabolic process	2	16
GO:1901160	primary amino compound metabolic process	4	2
GO:1901162	primary amino compound biosynthetic process	5	2
GO:1901564	organonitrogen compound metabolic process	3	16
GO:1901566	organonitrogen compound biosynthetic process	4	2
GO:1901576	organic substance biosynthetic process	3	2
GO:1901615	organic hydroxy compound metabolic process	3	2
GO:1901617	organic hydroxy compound biosynthetic process	4	2

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	16
GO:0016301	kinase activity	4	16
GO:0016740	transferase activity	2	16
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	16
GO:0016780	phosphotransferase activity, for other substituted phosphate groups	4	16
GO:0033188	sphingomyelin synthase activity	5	6
GO:0047493	ceramide cholinephosphotransferase activity	5	2

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	327	331	PF00656	0.698
CLV_NRD_NRD_1	142	144	PF00675	0.394
CLV_PCSK_KEX2_1	142	144	PF00082	0.376
CLV_PCSK_SKI1_1	103	107	PF00082	0.337
CLV_PCSK_SKI1_1	132	136	PF00082	0.308
CLV_PCSK_SKI1_1	148	152	PF00082	0.326
CLV_PCSK_SKI1_1	157	161	PF00082	0.267
CLV_PCSK_SKI1_1	36	40	PF00082	0.403
CLV_PCSK_SKI1_1	78	82	PF00082	0.441
DEG_APCC_DBOX_1	35	43	PF00400	0.325
DOC_CKS1_1	321	326	PF01111	0.692
DOC_MAPK_DCC_7	109	119	PF00069	0.534
DOC_MAPK_DCC_7	64	73	PF00069	0.320
DOC_MAPK_gen_1	64	73	PF00069	0.301
DOC_MAPK_MEF2A_6	244	253	PF00069	0.349
DOC_MAPK_MEF2A_6	36	45	PF00069	0.298
DOC_MAPK_MEF2A_6	64	73	PF00069	0.316
DOC_MAPK_NFAT4_5	36	44	PF00069	0.403
DOC_PP1_RVXF_1	114	120	PF00149	0.630
DOC_PP4_FxxP_1	237	240	PF00568	0.547
DOC_USP7_MATH_1	260	264	PF00917	0.292
DOC_USP7_MATH_1	325	329	PF00917	0.629
DOC_WW_Pin1_4	232	237	PF00397	0.409
DOC_WW_Pin1_4	320	325	PF00397	0.692
LIG_14-3-3_CanoR_1	116	120	PF00244	0.564
LIG_14-3-3_CanoR_1	157	163	PF00244	0.298
LIG_14-3-3_CanoR_1	244	250	PF00244	0.317
LIG_14-3-3_CanoR_1	262	271	PF00244	0.166
LIG_BRCT_BRCA1_1	224	228	PF00533	0.364
LIG_eIF4E_1	265	271	PF01652	0.245
LIG_FHA_1	116	122	PF00498	0.639
LIG_FHA_1	16	22	PF00498	0.630
LIG_FHA_1	219	225	PF00498	0.298
LIG_FHA_1	264	270	PF00498	0.238
LIG_FHA_1	277	283	PF00498	0.289
LIG_FHA_1	84	90	PF00498	0.296
LIG_FHA_1	94	100	PF00498	0.399
LIG_FHA_2	311	317	PF00498	0.698
LIG_FHA_2	321	327	PF00498	0.714
LIG_FHA_2	4	10	PF00498	0.678
LIG_IRF3_LxIS_1	247	254	PF10401	0.360
LIG_LIR_Apic_2	235	240	PF02991	0.539
LIG_LIR_Gen_1	160	170	PF02991	0.329
LIG_LIR_Gen_1	254	260	PF02991	0.298
LIG_LIR_Nem_3	160	165	PF02991	0.343
LIG_LIR_Nem_3	233	237	PF02991	0.473
LIG_LIR_Nem_3	254	259	PF02991	0.327
LIG_LIR_Nem_3	279	283	PF02991	0.290
LIG_LIR_Nem_3	44	50	PF02991	0.358
LIG_MYND_1	236	240	PF01753	0.539
LIG_NRBOX	38	44	PF00104	0.434
LIG_Pex14_2	228	232	PF04695	0.404
LIG_Pex14_2	237	241	PF04695	0.521
LIG_SH2_CRK	162	166	PF00017	0.308
LIG_SH2_CRK	24	28	PF00017	0.576
LIG_SH2_CRK	256	260	PF00017	0.308
LIG_SH2_PTP2	274	277	PF00017	0.441
LIG_SH2_STAP1	162	166	PF00017	0.308
LIG_SH2_STAP1	265	269	PF00017	0.238
LIG_SH2_STAT5	256	259	PF00017	0.303
LIG_SH2_STAT5	265	268	PF00017	0.233
LIG_SH2_STAT5	274	277	PF00017	0.257
LIG_SH3_3	118	124	PF00018	0.538
LIG_SH3_3	246	252	PF00018	0.451
LIG_SH3_3	318	324	PF00018	0.687
LIG_SUMO_SIM_anti_2	315	323	PF11976	0.704
LIG_SUMO_SIM_par_1	198	204	PF11976	0.292
LIG_SUMO_SIM_par_1	315	323	PF11976	0.664
LIG_SUMO_SIM_par_1	38	44	PF11976	0.381
LIG_TYR_ITIM	253	258	PF00017	0.364
LIG_TYR_ITSM	158	165	PF00017	0.404
LIG_UBA3_1	72	78	PF00899	0.397
LIG_WRC_WIRS_1	126	131	PF05994	0.603
MOD_CK1_1	218	224	PF00069	0.289
MOD_CK1_1	263	269	PF00069	0.281
MOD_CK1_1	3	9	PF00069	0.677
MOD_CK2_1	310	316	PF00069	0.600
MOD_CK2_1	320	326	PF00069	0.593
MOD_CMANNOS	300	303	PF00535	0.408
MOD_Cter_Amidation	140	143	PF01082	0.400
MOD_GlcNHglycan	139	142	PF01048	0.385
MOD_GlcNHglycan	206	209	PF01048	0.533
MOD_GlcNHglycan	217	220	PF01048	0.235
MOD_GlcNHglycan	330	334	PF01048	0.515
MOD_GSK3_1	174	181	PF00069	0.339
MOD_GSK3_1	218	225	PF00069	0.268
MOD_GSK3_1	325	332	PF00069	0.694
MOD_N-GLC_1	83	88	PF02516	0.524
MOD_N-GLC_1	93	98	PF02516	0.399
MOD_N-GLC_2	183	185	PF02516	0.555
MOD_NEK2_1	158	163	PF00069	0.286
MOD_NEK2_1	174	179	PF00069	0.378
MOD_NEK2_1	215	220	PF00069	0.289
MOD_NEK2_1	222	227	PF00069	0.289
MOD_NEK2_1	276	281	PF00069	0.389
MOD_NEK2_1	41	46	PF00069	0.355
MOD_NEK2_1	72	77	PF00069	0.389
MOD_NEK2_1	93	98	PF00069	0.417
MOD_PIKK_1	29	35	PF00454	0.468
MOD_PKA_2	115	121	PF00069	0.595
MOD_Plk_1	93	99	PF00069	0.430
MOD_Plk_4	115	121	PF00069	0.617
MOD_Plk_4	125	131	PF00069	0.525
MOD_Plk_4	198	204	PF00069	0.298
MOD_Plk_4	218	224	PF00069	0.111
MOD_Plk_4	245	251	PF00069	0.260
MOD_Plk_4	41	47	PF00069	0.313
MOD_Plk_4	94	100	PF00069	0.270
MOD_ProDKin_1	232	238	PF00069	0.409
MOD_ProDKin_1	320	326	PF00069	0.694
TRG_ENDOCYTIC_2	162	165	PF00928	0.303
TRG_ENDOCYTIC_2	234	237	PF00928	0.440
TRG_ENDOCYTIC_2	255	258	PF00928	0.298
TRG_ENDOCYTIC_2	274	277	PF00928	0.211
TRG_ENDOCYTIC_2	280	283	PF00928	0.298
TRG_ER_diArg_1	142	144	PF00400	0.607
TRG_Pf-PMV_PEXEL_1	66	70	PF00026	0.554

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HRL7	Leptomonas seymouri	71%	88%
A0A0S4J1W3	Bodo saltans	45%	97%
A0A1X0P4U9	Trypanosomatidae	50%	96%
A0A3S7XA07	Leishmania donovani	92%	88%
A0A422NCN9	Trypanosoma rangeli	45%	100%
A4HNG1	Leishmania braziliensis	71%	88%
A4IC70	Leishmania infantum	93%	88%
B3A0L9	Trypanosoma brucei brucei	43%	95%
B3A0M0	Trypanosoma brucei brucei	43%	100%
B3A0M1	Trypanosoma brucei brucei	44%	100%
B3A0M2	Trypanosoma brucei brucei	43%	93%
C9ZYF5	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	44%	97%
C9ZYF7	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	43%	100%
C9ZYF8	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	44%	100%
C9ZYF9	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	44%	100%
C9ZYG0	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	44%	100%
C9ZYG2	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	43%	100%
E9AFX2	Leishmania major	100%	100%
E9B725	Leishmania mexicana (strain MHOM/GT/2001/U1103)	87%	88%
Q20696	Caenorhabditis elegans	22%	70%
Q20735	Caenorhabditis elegans	27%	100%
Q38E53	Trypanosoma brucei brucei (strain 927/4 GUTat10.1)	43%	95%
Q38E54	Trypanosoma brucei brucei (strain 927/4 GUTat10.1)	43%	100%
Q38E55	Trypanosoma brucei brucei (strain 927/4 GUTat10.1)	45%	100%
Q38E56	Trypanosoma brucei brucei (strain 927/4 GUTat10.1)	44%	93%
Q4E4I4	Trypanosoma cruzi (strain CL Brener)	52%	100%
Q96LT4	Homo sapiens	25%	81%
Q9DA37	Mus musculus	25%	71%