NAD-dependent protein deacetylase SIR2rp1

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Silent information regulator 2 related protein 1

Species:

Leishmania major

UniProt:

SIR2_LEIMA

TriTrypDb:

LmjF.26.0210 , LMJLV39_260007000 , LMJSD75_260006900

Length:

373

Annotations

LeishMANIAdb interaction annotations

by homology
Contact email: ipemberton@photeomix.com
Publication title: The crystal structure of the Leishmania infantum Silent Information Regulator 2 related protein 1: Implications to protein function and drug design
Publication 1st author(s): Ronin
Publication Identifier(s): 29543820
Host organism: -1
Interaction detection method(s): x-ray crystallography
Interaction type: direct interaction
Identification method participant A: predetermined participant
Identification method participant B: predetermined participant
ID(s) interactor A: A0A6L0XH39
ID(s) interactor B: P04637
Taxid interactor A: Leishmania infantum
Taxid interactor B: Homo sapiens
Biological role(s) interactor A: unspecified role
Biological role(s) interactor B: unspecified role
Experimental role(s) interactor A: neutral component

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	2
GO:0005829	cytosol	2	2
GO:0005856	cytoskeleton	5	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043228	non-membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0043232	intracellular non-membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

Q25337

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q25337

PDB structure(s): 5ol0_A , 5ol0_B

Function

Biological processes
Term	Name	Level	Count
GO:0001775	cell activation	2	2
GO:0002274	myeloid leukocyte activation	3	2
GO:0002376	immune system process	1	2
GO:0006139	nucleobase-containing compound metabolic process	3	2
GO:0006259	DNA metabolic process	4	2
GO:0006281	DNA repair	5	2
GO:0006725	cellular aromatic compound metabolic process	3	2
GO:0006807	nitrogen compound metabolic process	2	2
GO:0006950	response to stress	2	2
GO:0006974	DNA damage response	4	2
GO:0008152	metabolic process	1	2
GO:0009605	response to external stimulus	2	2
GO:0009607	response to biotic stimulus	2	2
GO:0009987	cellular process	1	2
GO:0033554	cellular response to stress	3	2
GO:0034641	cellular nitrogen compound metabolic process	3	2
GO:0042113	B cell activation	4	2
GO:0042116	macrophage activation	4	2
GO:0043170	macromolecule metabolic process	3	2
GO:0043207	response to external biotic stimulus	3	2
GO:0044237	cellular metabolic process	2	2
GO:0044238	primary metabolic process	2	2
GO:0044260	obsolete cellular macromolecule metabolic process	3	2
GO:0044403	biological process involved in symbiotic interaction	2	2
GO:0044419	biological process involved in interspecies interaction between organisms	1	2
GO:0045321	leukocyte activation	2	2
GO:0046483	heterocycle metabolic process	3	2
GO:0046649	lymphocyte activation	3	2
GO:0050896	response to stimulus	1	2
GO:0051701	biological process involved in interaction with host	3	2
GO:0051707	response to other organism	2	2
GO:0051716	cellular response to stimulus	2	2
GO:0052167	modulation by symbiont of host innate immune response	7	2
GO:0052173	response to defenses of other organism	3	2
GO:0052200	response to host defenses	4	2
GO:0052553	modulation by symbiont of host immune response	6	2
GO:0052572	response to host immune response	5	2
GO:0071704	organic substance metabolic process	2	2
GO:0075136	response to host	3	2
GO:0090304	nucleic acid metabolic process	4	2
GO:1901360	organic cyclic compound metabolic process	3	2

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	11
GO:0003824	catalytic activity	1	11
GO:0003950	NAD+ ADP-ribosyltransferase activity	5	2
GO:0004407	histone deacetylase activity	4	9
GO:0005488	binding	1	11
GO:0016740	transferase activity	2	11
GO:0016757	glycosyltransferase activity	3	5
GO:0016763	pentosyltransferase activity	4	2
GO:0016787	hydrolase activity	2	10
GO:0016810	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds	3	9
GO:0016811	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides	4	9
GO:0017136	NAD-dependent histone deacetylase activity	5	9
GO:0019213	deacetylase activity	3	9
GO:0033558	protein lysine deacetylase activity	3	9
GO:0034979	NAD-dependent protein deacetylase activity	4	9
GO:0036094	small molecule binding	2	11
GO:0042903	tubulin deacetylase activity	4	2
GO:0043167	ion binding	2	11
GO:0043168	anion binding	3	11
GO:0043169	cation binding	3	9
GO:0046872	metal ion binding	4	9
GO:0051287	NAD binding	4	11
GO:0070403	NAD+ binding	4	11
GO:0097159	organic cyclic compound binding	2	11
GO:0140096	catalytic activity, acting on a protein	2	9
GO:1901265	nucleoside phosphate binding	3	11
GO:1901363	heterocyclic compound binding	2	11
GO:0008270	zinc ion binding	6	7
GO:0046914	transition metal ion binding	5	7

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	308	312	PF00656	0.567
CLV_NRD_NRD_1	284	286	PF00675	0.714
CLV_NRD_NRD_1	32	34	PF00675	0.417
CLV_NRD_NRD_1	366	368	PF00675	0.526
CLV_PCSK_KEX2_1	284	286	PF00082	0.644
CLV_PCSK_KEX2_1	32	34	PF00082	0.371
CLV_PCSK_KEX2_1	354	356	PF00082	0.433
CLV_PCSK_PC1ET2_1	354	356	PF00082	0.430
CLV_PCSK_SKI1_1	32	36	PF00082	0.438
CLV_PCSK_SKI1_1	327	331	PF00082	0.340
CLV_PCSK_SKI1_1	349	353	PF00082	0.462
DEG_APCC_DBOX_1	31	39	PF00400	0.394
DEG_SPOP_SBC_1	304	308	PF00917	0.666
DOC_MAPK_FxFP_2	250	253	PF00069	0.362
DOC_MAPK_gen_1	242	250	PF00069	0.366
DOC_MAPK_gen_1	27	36	PF00069	0.443
DOC_MAPK_HePTP_8	26	38	PF00069	0.330
DOC_MAPK_MEF2A_6	233	240	PF00069	0.319
DOC_MAPK_MEF2A_6	29	38	PF00069	0.396
DOC_PP1_RVXF_1	325	332	PF00149	0.361
DOC_PP4_FxxP_1	250	253	PF00568	0.362
DOC_USP7_MATH_1	143	147	PF00917	0.414
DOC_USP7_MATH_1	279	283	PF00917	0.672
DOC_USP7_MATH_1	304	308	PF00917	0.602
DOC_USP7_MATH_1	40	44	PF00917	0.498
DOC_WW_Pin1_4	1	6	PF00397	0.688
DOC_WW_Pin1_4	133	138	PF00397	0.429
DOC_WW_Pin1_4	154	159	PF00397	0.469
DOC_WW_Pin1_4	275	280	PF00397	0.717
DOC_WW_Pin1_4	293	298	PF00397	0.624
LIG_14-3-3_CanoR_1	120	129	PF00244	0.444
LIG_14-3-3_CanoR_1	175	183	PF00244	0.462
LIG_14-3-3_CanoR_1	270	279	PF00244	0.740
LIG_14-3-3_CanoR_1	52	59	PF00244	0.365
LIG_Actin_WH2_2	335	351	PF00022	0.255
LIG_FHA_1	121	127	PF00498	0.413
LIG_FHA_2	257	263	PF00498	0.583
LIG_FHA_2	367	373	PF00498	0.718
LIG_GBD_Chelix_1	211	219	PF00786	0.301
LIG_HP1_1	236	240	PF01393	0.302
LIG_Integrin_RGD_1	332	334	PF01839	0.477
LIG_LIR_Apic_2	318	323	PF02991	0.330
LIG_LIR_Gen_1	196	207	PF02991	0.444
LIG_LIR_Gen_1	56	67	PF02991	0.402
LIG_LIR_Nem_3	196	202	PF02991	0.426
LIG_LIR_Nem_3	310	316	PF02991	0.491
LIG_LIR_Nem_3	56	62	PF02991	0.402
LIG_LIR_Nem_3	84	89	PF02991	0.413
LIG_Pex14_1	96	100	PF04695	0.413
LIG_REV1ctd_RIR_1	328	336	PF16727	0.242
LIG_RPA_C_Fungi	237	249	PF08784	0.345
LIG_SH2_CRK	59	63	PF00017	0.233
LIG_SH2_NCK_1	320	324	PF00017	0.322
LIG_SH2_SRC	165	168	PF00017	0.316
LIG_SH2_SRC	320	323	PF00017	0.313
LIG_SH2_STAT5	165	168	PF00017	0.438
LIG_SH2_STAT5	25	28	PF00017	0.319
LIG_SH3_3	2	8	PF00018	0.600
LIG_SH3_3	250	256	PF00018	0.522
LIG_SUMO_SIM_anti_2	210	218	PF11976	0.253
LIG_SUMO_SIM_anti_2	75	81	PF11976	0.275
LIG_SUMO_SIM_par_1	210	218	PF11976	0.334
LIG_TRAF2_1	297	300	PF00917	0.609
LIG_TRAF2_1	8	11	PF00917	0.512
LIG_TRFH_1	224	228	PF08558	0.303
MOD_CDK_SPK_2	1	6	PF00069	0.568
MOD_CK1_1	174	180	PF00069	0.427
MOD_CK1_1	271	277	PF00069	0.779
MOD_CK1_1	305	311	PF00069	0.548
MOD_CK1_1	53	59	PF00069	0.233
MOD_CK2_1	293	299	PF00069	0.704
MOD_CK2_1	366	372	PF00069	0.563
MOD_GlcNHglycan	169	172	PF01048	0.316
MOD_GlcNHglycan	279	282	PF01048	0.693
MOD_GlcNHglycan	358	361	PF01048	0.595
MOD_GSK3_1	167	174	PF00069	0.391
MOD_GSK3_1	269	276	PF00069	0.632
MOD_GSK3_1	287	294	PF00069	0.534
MOD_GSK3_1	302	309	PF00069	0.608
MOD_GSK3_1	362	369	PF00069	0.725
MOD_GSK3_1	68	75	PF00069	0.275
MOD_NEK2_1	169	174	PF00069	0.286
MOD_NEK2_1	356	361	PF00069	0.462
MOD_NEK2_1	74	79	PF00069	0.296
MOD_NEK2_2	256	261	PF00069	0.469
MOD_PIKK_1	120	126	PF00454	0.249
MOD_PIKK_1	271	277	PF00454	0.722
MOD_PKA_1	367	373	PF00069	0.526
MOD_PKA_2	174	180	PF00069	0.416
MOD_PKA_2	269	275	PF00069	0.724
MOD_PKA_2	356	362	PF00069	0.474
MOD_PKA_2	366	372	PF00069	0.559
MOD_PKA_2	51	57	PF00069	0.181
MOD_Plk_2-3	287	293	PF00069	0.569
MOD_Plk_4	74	80	PF00069	0.285
MOD_ProDKin_1	1	7	PF00069	0.676
MOD_ProDKin_1	133	139	PF00069	0.271
MOD_ProDKin_1	154	160	PF00069	0.327
MOD_ProDKin_1	275	281	PF00069	0.716
MOD_ProDKin_1	293	299	PF00069	0.627
TRG_DiLeu_BaLyEn_6	107	112	PF01217	0.293
TRG_DiLeu_BaLyEn_6	114	119	PF01217	0.239
TRG_ENDOCYTIC_2	59	62	PF00928	0.233
TRG_ER_diArg_1	31	33	PF00400	0.416
TRG_ER_diArg_1	355	358	PF00400	0.517
TRG_Pf-PMV_PEXEL_1	110	114	PF00026	0.381
TRG_Pf-PMV_PEXEL_1	24	28	PF00026	0.354

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HVQ5	Leptomonas seymouri	67%	98%
A0A1X0NT02	Trypanosomatidae	55%	100%
A0A3Q8IDH7	Leishmania donovani	92%	100%
A0A422NVE3	Trypanosoma rangeli	56%	100%
A0A6L0XH39	Leishmania infantum	92%	100%
A8NWP2	Coprinopsis cinerea (strain Okayama-7 / 130 / ATCC MYA-4618 / FGSC 9003)	28%	100%
B0RM75	Xanthomonas campestris pv. campestris (strain B100)	29%	100%
B2RZ55	Rattus norvegicus	25%	93%
C8V3W5	Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139)	39%	76%
C9ZS62	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	51%	100%
E1BRE2	Gallus gallus	29%	100%
E2RDZ6	Canis lupus familiaris	31%	100%
E9AIR9	Leishmania braziliensis	78%	100%
E9AY03	Leishmania mexicana (strain MHOM/GT/2001/U1103)	91%	100%
F7DKV7	Xenopus tropicalis	30%	100%
F7EZ75	Macaca mulatta	31%	100%
O94640	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	37%	79%
P06700	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	37%	66%
P0CS88	Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139)	38%	100%
P53686	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	39%	100%
P53687	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	30%	83%
Q0P595	Bos taurus	25%	93%
Q1JQC6	Bos taurus	28%	100%
Q25337	Leishmania major	100%	100%
Q3ZBQ0	Bos taurus	30%	100%
Q4UZX0	Xanthomonas campestris pv. campestris (strain 8004)	29%	100%
Q54QE6	Dictyostelium discoideum	46%	73%
Q57V41	Trypanosoma brucei brucei (strain 927/4 GUTat10.1)	51%	100%
Q5A1W9	Candida albicans (strain SC5314 / ATCC MYA-2876)	28%	77%
Q5R6G3	Pongo abelii	31%	100%
Q68F47	Xenopus laevis	29%	100%
Q68FX9	Rattus norvegicus	28%	100%
Q6FWI7	Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65)	37%	73%
Q757M7	Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)	37%	67%
Q882K4	Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)	27%	100%
Q89EA6	Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110)	30%	100%
Q8BKJ9	Mus musculus	25%	93%
Q8K2C6	Mus musculus	30%	100%
Q8PDM9	Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25)	29%	100%
Q8PQK3	Xanthomonas axonopodis pv. citri (strain 306)	31%	100%
Q9NXA8	Homo sapiens	31%	100%
Q9USN7	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	39%	100%
Q9Y6E7	Homo sapiens	28%	100%
V5BZF3	Trypanosoma cruzi	55%	100%