Putative calpain-like cysteine peptidase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

cysteine peptidase, Clan CA, family C2, putative

Species:

Leishmania major

UniProt:

Q9U0T9_LEIMA

TriTrypDb:

LmjF.04.0450 , LMJLV39_040009300 , LMJSD75_040009600

Length:

854

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 1
Forrest at al. (metacyclic)	no	yes: 3
Forrest at al. (procyclic)	no	yes: 4
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 36

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	yes	yes: 16

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 20

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 2, no: 102
NetGPI	no	yes: 0, no: 104

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	3
GO:0110165	cellular anatomical entity	1	5
GO:0005815	microtubule organizing center	2	1
GO:0005856	cytoskeleton	5	1
GO:0020016	ciliary pocket	2	1
GO:0020038	subpellicular network	2	1
GO:0030863	cortical cytoskeleton	6	1
GO:0036064	ciliary basal body	3	1
GO:0043226	organelle	2	1
GO:0043228	non-membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043232	intracellular non-membrane-bounded organelle	4	1
GO:0005930	axoneme	2	1
GO:0016020	membrane	2	1

Expansion

Sequence features

Q9U0T9

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q9U0T9

Function

Biological processes
Term	Name	Level	Count
GO:0006508	proteolysis	4	105
GO:0006807	nitrogen compound metabolic process	2	105
GO:0008152	metabolic process	1	105
GO:0019538	protein metabolic process	3	105
GO:0043170	macromolecule metabolic process	3	105
GO:0044238	primary metabolic process	2	105
GO:0071704	organic substance metabolic process	2	105
GO:1901564	organonitrogen compound metabolic process	3	105

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	105
GO:0004175	endopeptidase activity	4	105
GO:0004197	cysteine-type endopeptidase activity	5	105
GO:0004198	calcium-dependent cysteine-type endopeptidase activity	6	105
GO:0008233	peptidase activity	3	105
GO:0008234	cysteine-type peptidase activity	4	105
GO:0016787	hydrolase activity	2	105
GO:0140096	catalytic activity, acting on a protein	2	105

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	273	277	PF00656	0.523
CLV_C14_Caspase3-7	796	800	PF00656	0.596
CLV_NRD_NRD_1	488	490	PF00675	0.440
CLV_NRD_NRD_1	84	86	PF00675	0.555
CLV_PCSK_KEX2_1	312	314	PF00082	0.369
CLV_PCSK_PC1ET2_1	312	314	PF00082	0.394
CLV_PCSK_SKI1_1	309	313	PF00082	0.353
CLV_PCSK_SKI1_1	400	404	PF00082	0.390
CLV_PCSK_SKI1_1	86	90	PF00082	0.657
DEG_SCF_FBW7_1	124	130	PF00400	0.539
DOC_CDC14_PxL_1	734	742	PF14671	0.464
DOC_CKS1_1	124	129	PF01111	0.841
DOC_CKS1_1	496	501	PF01111	0.537
DOC_CYCLIN_yClb1_LxF_4	286	292	PF00134	0.501
DOC_CYCLIN_yCln2_LP_2	554	560	PF00134	0.614
DOC_MAPK_gen_1	781	789	PF00069	0.510
DOC_MAPK_gen_1	90	100	PF00069	0.421
DOC_MAPK_MEF2A_6	643	650	PF00069	0.458
DOC_MAPK_MEF2A_6	781	789	PF00069	0.506
DOC_MAPK_MEF2A_6	93	102	PF00069	0.693
DOC_PP1_RVXF_1	135	141	PF00149	0.707
DOC_PP1_RVXF_1	388	394	PF00149	0.545
DOC_PP1_RVXF_1	481	488	PF00149	0.599
DOC_PP2B_LxvP_1	100	103	PF13499	0.585
DOC_PP2B_LxvP_1	684	687	PF13499	0.317
DOC_PP2B_LxvP_1	735	738	PF13499	0.447
DOC_PP4_FxxP_1	155	158	PF00568	0.364
DOC_PP4_FxxP_1	371	374	PF00568	0.548
DOC_PP4_FxxP_1	760	763	PF00568	0.394
DOC_PP4_FxxP_1	81	84	PF00568	0.616
DOC_USP7_MATH_1	298	302	PF00917	0.518
DOC_USP7_MATH_1	667	671	PF00917	0.602
DOC_USP7_MATH_2	506	512	PF00917	0.434
DOC_USP7_UBL2_3	433	437	PF12436	0.542
DOC_USP7_UBL2_3	542	546	PF12436	0.611
DOC_USP7_UBL2_3	86	90	PF12436	0.596
DOC_WW_Pin1_4	120	125	PF00397	0.695
DOC_WW_Pin1_4	193	198	PF00397	0.425
DOC_WW_Pin1_4	495	500	PF00397	0.550
DOC_WW_Pin1_4	509	514	PF00397	0.544
LIG_14-3-3_CanoR_1	167	173	PF00244	0.469
LIG_14-3-3_CanoR_1	390	394	PF00244	0.522
LIG_14-3-3_CanoR_1	624	630	PF00244	0.481
LIG_14-3-3_CanoR_1	775	781	PF00244	0.700
LIG_14-3-3_CanoR_1	85	92	PF00244	0.628
LIG_APCC_ABBA_1	826	831	PF00400	0.401
LIG_BIR_II_1	1	5	PF00653	0.819
LIG_BRCT_BRCA1_1	177	181	PF00533	0.380
LIG_BRCT_BRCA1_1	19	23	PF00533	0.765
LIG_BRCT_BRCA1_1	511	515	PF00533	0.654
LIG_deltaCOP1_diTrp_1	710	715	PF00928	0.508
LIG_FHA_1	146	152	PF00498	0.412
LIG_FHA_1	526	532	PF00498	0.547
LIG_FHA_1	592	598	PF00498	0.580
LIG_FHA_1	647	653	PF00498	0.526
LIG_FHA_1	722	728	PF00498	0.532
LIG_FHA_1	821	827	PF00498	0.531
LIG_FHA_2	194	200	PF00498	0.371
LIG_FHA_2	202	208	PF00498	0.283
LIG_FHA_2	273	279	PF00498	0.514
LIG_FHA_2	357	363	PF00498	0.499
LIG_FHA_2	4	10	PF00498	0.706
LIG_FHA_2	598	604	PF00498	0.582
LIG_FHA_2	626	632	PF00498	0.494
LIG_LIR_Apic_2	152	158	PF02991	0.360
LIG_LIR_Apic_2	759	763	PF02991	0.402
LIG_LIR_Apic_2	78	84	PF02991	0.734
LIG_LIR_Gen_1	432	443	PF02991	0.547
LIG_LIR_Gen_1	595	605	PF02991	0.550
LIG_LIR_Gen_1	710	721	PF02991	0.492
LIG_LIR_Gen_1	731	741	PF02991	0.440
LIG_LIR_Gen_1	769	777	PF02991	0.704
LIG_LIR_Nem_3	171	175	PF02991	0.374
LIG_LIR_Nem_3	293	299	PF02991	0.504
LIG_LIR_Nem_3	405	411	PF02991	0.568
LIG_LIR_Nem_3	425	430	PF02991	0.539
LIG_LIR_Nem_3	441	445	PF02991	0.534
LIG_LIR_Nem_3	595	601	PF02991	0.536
LIG_LIR_Nem_3	676	680	PF02991	0.483
LIG_LIR_Nem_3	692	698	PF02991	0.425
LIG_LIR_Nem_3	710	716	PF02991	0.514
LIG_LIR_Nem_3	731	737	PF02991	0.414
LIG_LIR_Nem_3	769	773	PF02991	0.690
LIG_LIR_Nem_3	816	821	PF02991	0.584
LIG_LIR_Nem_3	94	98	PF02991	0.560
LIG_LYPXL_yS_3	677	680	PF13949	0.419
LIG_Pex14_2	226	230	PF04695	0.427
LIG_RPA_C_Fungi	770	782	PF08784	0.445
LIG_SH2_CRK	442	446	PF00017	0.382
LIG_SH2_CRK	492	496	PF00017	0.414
LIG_SH2_CRK	695	699	PF00017	0.603
LIG_SH2_GRB2like	766	769	PF00017	0.393
LIG_SH2_NCK_1	121	125	PF00017	0.744
LIG_SH2_NCK_1	442	446	PF00017	0.413
LIG_SH2_PTP2	322	325	PF00017	0.439
LIG_SH2_STAT5	212	215	PF00017	0.427
LIG_SH2_STAT5	247	250	PF00017	0.502
LIG_SH2_STAT5	322	325	PF00017	0.371
LIG_SH2_STAT5	407	410	PF00017	0.392
LIG_SH2_STAT5	607	610	PF00017	0.420
LIG_SH2_STAT5	734	737	PF00017	0.426
LIG_SH2_STAT5	766	769	PF00017	0.620
LIG_SH2_STAT5	834	837	PF00017	0.543
LIG_SH3_1	121	127	PF00018	0.511
LIG_SH3_3	121	127	PF00018	0.742
LIG_SH3_3	162	168	PF00018	0.254
LIG_SH3_3	35	41	PF00018	0.744
LIG_SH3_3	500	506	PF00018	0.388
LIG_SH3_3	639	645	PF00018	0.448
LIG_SH3_3	68	74	PF00018	0.779
LIG_SH3_3	816	822	PF00018	0.575
LIG_SUMO_SIM_anti_2	6	12	PF11976	0.501
LIG_SUMO_SIM_par_1	537	543	PF11976	0.522
LIG_TRAF2_1	67	70	PF00917	0.591
LIG_TRAF2_1	828	831	PF00917	0.621
LIG_TRFH_1	734	738	PF08558	0.438
LIG_TYR_ITIM	160	165	PF00017	0.405
LIG_WRC_WIRS_1	186	191	PF05994	0.481
LIG_WW_3	90	94	PF00397	0.654
MOD_CDK_SPxK_1	495	501	PF00069	0.408
MOD_CK1_1	12	18	PF00069	0.733
MOD_CK1_1	123	129	PF00069	0.708
MOD_CK1_1	193	199	PF00069	0.378
MOD_CK1_1	236	242	PF00069	0.399
MOD_CK1_1	302	308	PF00069	0.387
MOD_CK1_1	470	476	PF00069	0.364
MOD_CK1_1	562	568	PF00069	0.438
MOD_CK1_1	776	782	PF00069	0.717
MOD_CK2_1	193	199	PF00069	0.370
MOD_CK2_1	201	207	PF00069	0.348
MOD_CK2_1	272	278	PF00069	0.512
MOD_CK2_1	298	304	PF00069	0.423
MOD_CK2_1	356	362	PF00069	0.375
MOD_GlcNHglycan	238	241	PF01048	0.473
MOD_GlcNHglycan	304	307	PF01048	0.404
MOD_GlcNHglycan	378	381	PF01048	0.397
MOD_GlcNHglycan	446	449	PF01048	0.390
MOD_GlcNHglycan	475	478	PF01048	0.367
MOD_GlcNHglycan	561	564	PF01048	0.459
MOD_GlcNHglycan	63	66	PF01048	0.791
MOD_GlcNHglycan	775	778	PF01048	0.748
MOD_GSK3_1	123	130	PF00069	0.700
MOD_GSK3_1	145	152	PF00069	0.414
MOD_GSK3_1	298	305	PF00069	0.378
MOD_GSK3_1	509	516	PF00069	0.416
MOD_GSK3_1	646	653	PF00069	0.508
MOD_GSK3_1	699	706	PF00069	0.467
MOD_GSK3_1	771	778	PF00069	0.623
MOD_N-GLC_1	12	17	PF02516	0.667
MOD_N-GLC_1	168	173	PF02516	0.515
MOD_N-GLC_1	175	180	PF02516	0.388
MOD_N-GLC_1	699	704	PF02516	0.488
MOD_NEK2_1	198	203	PF00069	0.351
MOD_NEK2_1	214	219	PF00069	0.458
MOD_NEK2_1	398	403	PF00069	0.423
MOD_NEK2_1	438	443	PF00069	0.397
MOD_NEK2_1	525	530	PF00069	0.388
MOD_NEK2_1	638	643	PF00069	0.457
MOD_NEK2_1	646	651	PF00069	0.419
MOD_NEK2_1	773	778	PF00069	0.650
MOD_PIKK_1	241	247	PF00454	0.521
MOD_PIKK_1	703	709	PF00454	0.555
MOD_PKA_2	389	395	PF00069	0.359
MOD_PKA_2	84	90	PF00069	0.561
MOD_Plk_1	12	18	PF00069	0.704
MOD_Plk_1	168	174	PF00069	0.406
MOD_Plk_1	214	220	PF00069	0.420
MOD_Plk_1	292	298	PF00069	0.389
MOD_Plk_1	592	598	PF00069	0.366
MOD_Plk_1	646	652	PF00069	0.448
MOD_Plk_1	743	749	PF00069	0.536
MOD_Plk_2-3	177	183	PF00069	0.343
MOD_Plk_4	12	18	PF00069	0.724
MOD_Plk_4	214	220	PF00069	0.398
MOD_Plk_4	362	368	PF00069	0.426
MOD_Plk_4	534	540	PF00069	0.418
MOD_Plk_4	592	598	PF00069	0.396
MOD_Plk_4	646	652	PF00069	0.436
MOD_ProDKin_1	120	126	PF00069	0.705
MOD_ProDKin_1	193	199	PF00069	0.425
MOD_ProDKin_1	495	501	PF00069	0.399
MOD_ProDKin_1	509	515	PF00069	0.391
MOD_SUMO_for_1	323	326	PF00179	0.489
MOD_SUMO_rev_2	537	544	PF00179	0.455
TRG_DiLeu_BaEn_1	156	161	PF01217	0.401
TRG_DiLeu_BaEn_2	221	227	PF01217	0.321
TRG_DiLeu_BaEn_4	156	162	PF01217	0.401
TRG_DiLeu_BaLyEn_6	547	552	PF01217	0.463
TRG_ENDOCYTIC_2	162	165	PF00928	0.384
TRG_ENDOCYTIC_2	407	410	PF00928	0.419
TRG_ENDOCYTIC_2	442	445	PF00928	0.385
TRG_ENDOCYTIC_2	492	495	PF00928	0.392
TRG_ENDOCYTIC_2	536	539	PF00928	0.422
TRG_ENDOCYTIC_2	677	680	PF00928	0.453
TRG_ENDOCYTIC_2	695	698	PF00928	0.339
TRG_ENDOCYTIC_2	734	737	PF00928	0.414
TRG_ENDOCYTIC_2	770	773	PF00928	0.462
TRG_ER_diArg_1	318	321	PF00400	0.423
TRG_ER_diArg_1	334	337	PF00400	0.405
TRG_Pf-PMV_PEXEL_1	421	425	PF00026	0.407
TRG_Pf-PMV_PEXEL_1	51	56	PF00026	0.799

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P8T6	Leptomonas seymouri	31%	100%
A0A0N1I8N2	Leptomonas seymouri	28%	87%
A0A0N1IGQ2	Leptomonas seymouri	31%	100%
A0A0N1ILF1	Leptomonas seymouri	35%	100%
A0A0N1IMH1	Leptomonas seymouri	31%	100%
A0A0N1P9P1	Leptomonas seymouri	72%	100%
A0A0N1PCA9	Leptomonas seymouri	33%	100%
A0A0N1PE91	Leptomonas seymouri	27%	96%
A0A0N1PFI4	Leptomonas seymouri	35%	100%
A0A0S4JLK6	Bodo saltans	28%	100%
A0A0S4JS70	Bodo saltans	25%	100%
A0A0S4KGT2	Bodo saltans	35%	100%
A0A1X0NJ19	Trypanosomatidae	28%	70%
A0A1X0NJK2	Trypanosomatidae	34%	100%
A0A1X0NJX8	Trypanosomatidae	31%	100%
A0A1X0NKT7	Trypanosomatidae	31%	100%
A0A1X0NKX8	Trypanosomatidae	32%	100%
A0A1X0NMT3	Trypanosomatidae	48%	100%
A0A1X0NW84	Trypanosomatidae	35%	100%
A0A1X0NW85	Trypanosomatidae	34%	100%
A0A1X0NW89	Trypanosomatidae	44%	99%
A0A1X0NWA6	Trypanosomatidae	29%	98%
A0A1X0NWW1	Trypanosomatidae	40%	100%
A0A3Q8IBS3	Leishmania donovani	41%	94%
A0A3Q8IDD4	Leishmania donovani	30%	100%
A0A3Q8IJT4	Leishmania donovani	27%	100%
A0A3S5H5A5	Leishmania donovani	96%	100%
A0A3S5ISG2	Trypanosoma rangeli	32%	100%
A0A3S7WW13	Leishmania donovani	28%	77%
A0A3S7WW18	Leishmania donovani	41%	100%
A0A3S7WW41	Leishmania donovani	31%	100%
A0A3S7WW71	Leishmania donovani	34%	100%
A0A3S7X430	Leishmania donovani	33%	100%
A0A3S7X438	Leishmania donovani	33%	91%
A0A3S7X460	Leishmania donovani	30%	100%
A0A3S7X463	Leishmania donovani	29%	91%
A0A3S7X470	Leishmania donovani	33%	100%
A0A422MYU1	Trypanosoma rangeli	44%	93%
A0A422MYX0	Trypanosoma rangeli	35%	100%
A4H3W4	Leishmania braziliensis	84%	100%
A4HE81	Leishmania braziliensis	31%	100%
A4HJ14	Leishmania braziliensis	33%	100%
A4HJ21	Leishmania braziliensis	28%	96%
A4HJ22	Leishmania braziliensis	31%	90%
A4HJ24	Leishmania braziliensis	31%	100%
A4HS39	Leishmania infantum	96%	100%
A4HYN0	Leishmania infantum	41%	100%
A4HYW1	Leishmania infantum	41%	84%
A4HYW2	Leishmania infantum	34%	100%
A4HYW3	Leishmania infantum	31%	100%
A4HYW4	Leishmania infantum	28%	85%
A4I1J4	Leishmania infantum	30%	100%
A4I6E4	Leishmania infantum	33%	100%
A4I6E6	Leishmania infantum	30%	100%
A4I6F0	Leishmania infantum	33%	91%
A4I6K4	Leishmania infantum	33%	100%
A4I6K5	Leishmania infantum	28%	91%
A4I6K6	Leishmania infantum	27%	100%
C9ZIE7	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	42%	79%
C9ZIE8	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	35%	100%
C9ZIE9	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	35%	100%
C9ZN52	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	32%	100%
C9ZN53	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	30%	100%
C9ZWY4	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	29%	100%
C9ZY36	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	42%	100%
E8NHG6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	33%	100%
E8NHG7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	34%	91%
E8NHG8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	31%	100%
E8NHM2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	31%	100%
E8NHM4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	31%	100%
E8NHM8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	31%	100%
E9AIH1	Leishmania braziliensis	41%	100%
E9AIH3	Leishmania braziliensis	35%	100%
E9AIH4	Leishmania braziliensis	33%	100%
E9AK26	Leishmania mexicana (strain MHOM/GT/2001/U1103)	93%	100%
E9AUQ7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	41%	91%
E9AUQ9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	34%	100%
E9AUR0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	30%	100%
E9AXM9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	31%	100%
E9B1J0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	34%	100%
E9B1J1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	28%	91%
E9B1J2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	27%	100%
E9B1J6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	31%	100%
Q4Q6L7	Leishmania major	30%	100%
Q4Q6L9	Leishmania major	34%	100%
Q4Q6M0	Leishmania major	33%	100%
Q4Q6M2	Leishmania major	27%	100%
Q4Q6M3	Leishmania major	28%	100%
Q4Q6M4	Leishmania major	34%	100%
Q4Q9U3	Leishmania major	30%	98%
Q4QCS6	Leishmania major	31%	99%
Q4QCS7	Leishmania major	34%	95%
Q4QCS8	Leishmania major	41%	100%
Q4QCS9	Leishmania major	40%	100%
V5AYJ1	Trypanosoma cruzi	30%	100%
V5B5I4	Trypanosoma cruzi	43%	100%
V5BA05	Trypanosoma cruzi	40%	100%
V5BEL3	Trypanosoma cruzi	34%	100%
V5BII7	Trypanosoma cruzi	28%	69%
V5BN20	Trypanosoma cruzi	32%	100%
V5D5V8	Trypanosoma cruzi	29%	100%
V5D9Y2	Trypanosoma cruzi	46%	100%
V5DES7	Trypanosoma cruzi	33%	100%