Acyltransferase-like protein, copy 2

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

acyltransferase-like protein, copy 2

Species:

Leishmania major

UniProt:

Q9NF90_LEIMA

TriTrypDb:

LmjF.04.1050 , LMJLV39_040016100 , LMJSD75_040016400

Length:

356

Annotations

LeishMANIAdb annotations

This conserved lipid acyltransferase has many re-entrant segments but only one true TM helix. Most closely related to bacterial acyltransferases.. Heavily expanded in kinetoplastids for unknown reasons. Localization: ER (by homology)

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 27

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 27
NetGPI	no	yes: 0, no: 27

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	23
GO:0110165	cellular anatomical entity	1	23

Expansion

Sequence features

Q9NF90

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q9NF90

Function

Biological processes
Term	Name	Level	Count
GO:0006629	lipid metabolic process	3	5
GO:0006644	phospholipid metabolic process	4	5
GO:0006650	glycerophospholipid metabolic process	5	5
GO:0006654	phosphatidic acid biosynthetic process	6	5
GO:0006793	phosphorus metabolic process	3	5
GO:0006796	phosphate-containing compound metabolic process	4	5
GO:0008152	metabolic process	1	5
GO:0008610	lipid biosynthetic process	4	5
GO:0008654	phospholipid biosynthetic process	5	5
GO:0009058	biosynthetic process	2	5
GO:0009987	cellular process	1	5
GO:0019637	organophosphate metabolic process	3	5
GO:0044237	cellular metabolic process	2	5
GO:0044238	primary metabolic process	2	5
GO:0044249	cellular biosynthetic process	3	5
GO:0044255	cellular lipid metabolic process	3	5
GO:0045017	glycerolipid biosynthetic process	4	5
GO:0046473	phosphatidic acid metabolic process	6	5
GO:0046474	glycerophospholipid biosynthetic process	5	5
GO:0046486	glycerolipid metabolic process	4	5
GO:0071704	organic substance metabolic process	2	5
GO:0090407	organophosphate biosynthetic process	4	5
GO:1901576	organic substance biosynthetic process	3	5

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	28
GO:0003841	1-acylglycerol-3-phosphate O-acyltransferase activity	7	6
GO:0008374	O-acyltransferase activity	5	6
GO:0016411	acylglycerol O-acyltransferase activity	6	6
GO:0016740	transferase activity	2	28
GO:0016746	acyltransferase activity	3	28
GO:0016747	acyltransferase activity, transferring groups other than amino-acyl groups	4	6
GO:0042171	lysophosphatidic acid acyltransferase activity	6	6
GO:0071617	lysophospholipid acyltransferase activity	5	6

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	188	192	PF00656	0.294
CLV_MEL_PAP_1	175	181	PF00089	0.524
CLV_NRD_NRD_1	166	168	PF00675	0.512
CLV_NRD_NRD_1	324	326	PF00675	0.626
CLV_NRD_NRD_1	81	83	PF00675	0.285
CLV_PCSK_FUR_1	321	325	PF00082	0.559
CLV_PCSK_KEX2_1	323	325	PF00082	0.664
CLV_PCSK_KEX2_1	81	83	PF00082	0.270
CLV_PCSK_SKI1_1	333	337	PF00082	0.531
CLV_PCSK_SKI1_1	51	55	PF00082	0.349
DOC_CYCLIN_yCln2_LP_2	70	76	PF00134	0.410
DOC_MAPK_gen_1	248	257	PF00069	0.312
DOC_MAPK_gen_1	81	89	PF00069	0.532
DOC_MAPK_MEF2A_6	293	301	PF00069	0.392
DOC_MAPK_MEF2A_6	82	91	PF00069	0.532
DOC_PP1_RVXF_1	95	102	PF00149	0.369
DOC_PP2B_LxvP_1	60	63	PF13499	0.419
DOC_PP2B_LxvP_1	77	80	PF13499	0.562
DOC_USP7_MATH_1	202	206	PF00917	0.200
DOC_WW_Pin1_4	147	152	PF00397	0.316
DOC_WW_Pin1_4	226	231	PF00397	0.265
LIG_14-3-3_CterR_2	353	356	PF00244	0.391
LIG_Actin_WH2_2	151	169	PF00022	0.343
LIG_AP2alpha_1	140	144	PF02296	0.259
LIG_BIR_III_4	189	193	PF00653	0.191
LIG_CSL_BTD_1	262	265	PF09270	0.365
LIG_FHA_1	335	341	PF00498	0.365
LIG_FHA_1	56	62	PF00498	0.316
LIG_FHA_2	241	247	PF00498	0.242
LIG_GBD_Chelix_1	295	303	PF00786	0.544
LIG_LIR_Gen_1	138	149	PF02991	0.283
LIG_LIR_Gen_1	240	249	PF02991	0.244
LIG_LIR_Gen_1	34	44	PF02991	0.465
LIG_LIR_Nem_3	138	144	PF02991	0.291
LIG_LIR_Nem_3	240	244	PF02991	0.261
LIG_LIR_Nem_3	34	39	PF02991	0.468
LIG_Pex14_2	140	144	PF04695	0.262
LIG_Pex14_2	172	176	PF04695	0.309
LIG_Pex14_2	182	186	PF04695	0.280
LIG_Pex14_2	92	96	PF04695	0.375
LIG_SH2_CRK	36	40	PF00017	0.309
LIG_SH2_STAT5	185	188	PF00017	0.249
LIG_SH2_STAT5	253	256	PF00017	0.271
LIG_SH2_STAT5	36	39	PF00017	0.288
LIG_SH2_STAT5	69	72	PF00017	0.361
LIG_SH2_STAT5	76	79	PF00017	0.570
LIG_SH3_3	216	222	PF00018	0.276
LIG_SUMO_SIM_par_1	253	260	PF11976	0.363
LIG_TRAF2_1	316	319	PF00917	0.319
LIG_TYR_ITIM	74	79	PF00017	0.464
LIG_UBA3_1	155	163	PF00899	0.200
LIG_UBA3_1	91	97	PF00899	0.299
LIG_WRC_WIRS_1	238	243	PF05994	0.277
LIG_WW_3	263	267	PF00397	0.274
MOD_CK1_1	256	262	PF00069	0.255
MOD_CK2_1	240	246	PF00069	0.269
MOD_CK2_1	325	331	PF00069	0.297
MOD_Cter_Amidation	321	324	PF01082	0.620
MOD_GlcNHglycan	64	67	PF01048	0.331
MOD_GSK3_1	157	164	PF00069	0.314
MOD_GSK3_1	349	356	PF00069	0.387
MOD_N-GLC_1	135	140	PF02516	0.473
MOD_N-GLC_1	226	231	PF02516	0.461
MOD_N-GLC_1	325	330	PF02516	0.619
MOD_NEK2_1	135	140	PF00069	0.257
MOD_NEK2_1	161	166	PF00069	0.295
MOD_NEK2_1	53	58	PF00069	0.341
MOD_NMyristoyl	1	7	PF02799	0.496
MOD_PIKK_1	202	208	PF00454	0.389
MOD_PK_1	325	331	PF00069	0.295
MOD_PKB_1	323	331	PF00069	0.296
MOD_Plk_1	135	141	PF00069	0.273
MOD_Plk_1	259	265	PF00069	0.349
MOD_Plk_4	136	142	PF00069	0.259
MOD_Plk_4	240	246	PF00069	0.311
MOD_Plk_4	325	331	PF00069	0.413
MOD_Plk_4	34	40	PF00069	0.458
MOD_ProDKin_1	147	153	PF00069	0.316
MOD_ProDKin_1	226	232	PF00069	0.265
MOD_SUMO_rev_2	328	335	PF00179	0.340
TRG_ENDOCYTIC_2	238	241	PF00928	0.324
TRG_ENDOCYTIC_2	252	255	PF00928	0.247
TRG_ENDOCYTIC_2	36	39	PF00928	0.351
TRG_ENDOCYTIC_2	76	79	PF00928	0.469
TRG_ER_diArg_1	248	251	PF00400	0.312
TRG_ER_diArg_1	321	324	PF00400	0.428
TRG_ER_diArg_1	39	42	PF00400	0.381
TRG_ER_diArg_1	80	82	PF00400	0.561

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P455	Leptomonas seymouri	64%	100%
A0A0N1HTP4	Leptomonas seymouri	54%	100%
A0A0N1I6V1	Leptomonas seymouri	35%	100%
A0A0N1IKX5	Leptomonas seymouri	33%	100%
A0A0S4IQ05	Bodo saltans	31%	100%
A0A0S4IR22	Bodo saltans	43%	100%
A0A1X0P9F0	Trypanosomatidae	35%	100%
A0A3R7NKA0	Trypanosoma rangeli	35%	100%
A0A3S5H5E4	Leishmania donovani	52%	82%
A0A3S5H7L8	Leishmania donovani	34%	100%
A0A3S7WNW6	Leishmania donovani	92%	100%
A4H419	Leishmania braziliensis	57%	100%
A4H420	Leishmania braziliensis	71%	100%
A4HHM7	Leishmania braziliensis	33%	100%
A4HKI5	Leishmania braziliensis	27%	100%
A4HSA1	Leishmania infantum	52%	100%
A4HSA2	Leishmania infantum	92%	100%
A4I4U1	Leishmania infantum	33%	100%
A4I4U2	Leishmania infantum	34%	100%
C9ZLI8	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	35%	100%
E9AE89	Leishmania major	33%	100%
E9AE90	Leishmania major	32%	100%
E9AK86	Leishmania mexicana (strain MHOM/GT/2001/U1103)	86%	100%
E9ALJ6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	32%	100%
Q9NF91	Leishmania major	53%	92%
V5C201	Trypanosoma cruzi	39%	100%