Possible serine/threonine kinase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Serine/threonine-protein kinase NEK10, putative

Species:

Leishmania major

UniProt:

Q9NE58_LEIMA

TriTrypDb:

LmjF.30.2130 , LMJLV39_300028200 , LMJSD75_300028000

Length:

983

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 9
NetGPI	no	yes: 0, no: 9

Could not find GO cellular_component term for this entry.

Expansion

Sequence features

Q9NE58

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q9NE58

Function

Biological processes
Term	Name	Level	Count
GO:0006468	protein phosphorylation	5	10
GO:0006508	proteolysis	4	2
GO:0006511	ubiquitin-dependent protein catabolic process	7	2
GO:0006793	phosphorus metabolic process	3	10
GO:0006796	phosphate-containing compound metabolic process	4	10
GO:0006807	nitrogen compound metabolic process	2	10
GO:0008152	metabolic process	1	10
GO:0009056	catabolic process	2	2
GO:0009057	macromolecule catabolic process	4	2
GO:0009987	cellular process	1	10
GO:0010941	regulation of cell death	4	2
GO:0016310	phosphorylation	5	10
GO:0016567	protein ubiquitination	7	2
GO:0019538	protein metabolic process	3	10
GO:0019941	modification-dependent protein catabolic process	6	2
GO:0032446	protein modification by small protein conjugation	6	2
GO:0036211	protein modification process	4	10
GO:0042981	regulation of apoptotic process	6	2
GO:0043067	regulation of programmed cell death	5	2
GO:0043170	macromolecule metabolic process	3	10
GO:0043412	macromolecule modification	4	10
GO:0043632	modification-dependent macromolecule catabolic process	5	2
GO:0044237	cellular metabolic process	2	10
GO:0044238	primary metabolic process	2	10
GO:0044248	cellular catabolic process	3	2
GO:0044260	obsolete cellular macromolecule metabolic process	3	2
GO:0044265	obsolete cellular macromolecule catabolic process	4	2
GO:0050789	regulation of biological process	2	2
GO:0050794	regulation of cellular process	3	2
GO:0051603	proteolysis involved in protein catabolic process	5	2
GO:0065007	biological regulation	1	2
GO:0070647	protein modification by small protein conjugation or removal	5	2
GO:0071704	organic substance metabolic process	2	10
GO:1901564	organonitrogen compound metabolic process	3	10
GO:1901575	organic substance catabolic process	3	2

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	10
GO:0003824	catalytic activity	1	10
GO:0004672	protein kinase activity	3	10
GO:0005488	binding	1	10
GO:0005524	ATP binding	5	10
GO:0008270	zinc ion binding	6	9
GO:0016301	kinase activity	4	10
GO:0016740	transferase activity	2	10
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	10
GO:0016773	phosphotransferase activity, alcohol group as acceptor	4	10
GO:0017076	purine nucleotide binding	4	10
GO:0030554	adenyl nucleotide binding	5	10
GO:0032553	ribonucleotide binding	3	10
GO:0032555	purine ribonucleotide binding	4	10
GO:0032559	adenyl ribonucleotide binding	5	10
GO:0035639	purine ribonucleoside triphosphate binding	4	10
GO:0036094	small molecule binding	2	10
GO:0043167	ion binding	2	10
GO:0043168	anion binding	3	10
GO:0043169	cation binding	3	9
GO:0046872	metal ion binding	4	9
GO:0046914	transition metal ion binding	5	9
GO:0097159	organic cyclic compound binding	2	10
GO:0097367	carbohydrate derivative binding	2	10
GO:0140096	catalytic activity, acting on a protein	2	10
GO:1901265	nucleoside phosphate binding	3	10
GO:1901363	heterocyclic compound binding	2	10

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	604	608	PF00656	0.565
CLV_NRD_NRD_1	567	569	PF00675	0.447
CLV_NRD_NRD_1	7	9	PF00675	0.444
CLV_NRD_NRD_1	77	79	PF00675	0.408
CLV_NRD_NRD_1	785	787	PF00675	0.408
CLV_NRD_NRD_1	839	841	PF00675	0.444
CLV_PCSK_KEX2_1	7	9	PF00082	0.444
CLV_PCSK_KEX2_1	77	79	PF00082	0.408
CLV_PCSK_PC1ET2_1	7	9	PF00082	0.444
CLV_PCSK_SKI1_1	225	229	PF00082	0.356
CLV_PCSK_SKI1_1	230	234	PF00082	0.331
CLV_PCSK_SKI1_1	243	247	PF00082	0.275
CLV_PCSK_SKI1_1	330	334	PF00082	0.365
CLV_PCSK_SKI1_1	336	340	PF00082	0.326
CLV_PCSK_SKI1_1	402	406	PF00082	0.657
CLV_PCSK_SKI1_1	48	52	PF00082	0.348
CLV_PCSK_SKI1_1	502	506	PF00082	0.762
CLV_PCSK_SKI1_1	568	572	PF00082	0.579
CLV_PCSK_SKI1_1	635	639	PF00082	0.662
CLV_PCSK_SKI1_1	787	791	PF00082	0.485
CLV_PCSK_SKI1_1	964	968	PF00082	0.444
DEG_Nend_UBRbox_2	1	3	PF02207	0.509
DOC_ANK_TNKS_1	7	14	PF00023	0.444
DOC_CKS1_1	264	269	PF01111	0.344
DOC_CYCLIN_RxL_1	452	460	PF00134	0.727
DOC_CYCLIN_RxL_1	631	641	PF00134	0.601
DOC_MAPK_gen_1	230	239	PF00069	0.344
DOC_MAPK_gen_1	24	34	PF00069	0.408
DOC_MAPK_gen_1	48	56	PF00069	0.351
DOC_MAPK_gen_1	657	667	PF00069	0.522
DOC_MAPK_MEF2A_6	48	56	PF00069	0.348
DOC_PP1_RVXF_1	453	460	PF00149	0.729
DOC_PP1_RVXF_1	972	978	PF00149	0.565
DOC_PP2B_LxvP_1	509	512	PF13499	0.728
DOC_PP2B_LxvP_1	545	548	PF13499	0.521
DOC_USP7_MATH_1	119	123	PF00917	0.731
DOC_USP7_MATH_1	129	133	PF00917	0.672
DOC_USP7_MATH_1	303	307	PF00917	0.344
DOC_USP7_MATH_1	347	351	PF00917	0.485
DOC_USP7_MATH_1	366	370	PF00917	0.658
DOC_USP7_MATH_1	374	378	PF00917	0.639
DOC_USP7_MATH_1	466	470	PF00917	0.810
DOC_USP7_MATH_1	512	516	PF00917	0.674
DOC_USP7_MATH_1	650	654	PF00917	0.742
DOC_USP7_MATH_1	710	714	PF00917	0.407
DOC_USP7_MATH_1	723	727	PF00917	0.449
DOC_USP7_MATH_1	81	85	PF00917	0.683
DOC_USP7_MATH_1	860	864	PF00917	0.444
DOC_USP7_MATH_1	87	91	PF00917	0.656
DOC_WW_Pin1_4	263	268	PF00397	0.344
DOC_WW_Pin1_4	345	350	PF00397	0.343
DOC_WW_Pin1_4	360	365	PF00397	0.577
DOC_WW_Pin1_4	442	447	PF00397	0.757
DOC_WW_Pin1_4	461	466	PF00397	0.573
DOC_WW_Pin1_4	595	600	PF00397	0.480
DOC_WW_Pin1_4	605	610	PF00397	0.573
DOC_WW_Pin1_4	692	697	PF00397	0.291
DOC_WW_Pin1_4	715	720	PF00397	0.485
DOC_WW_Pin1_4	787	792	PF00397	0.444
LIG_14-3-3_CanoR_1	24	34	PF00244	0.498
LIG_14-3-3_CanoR_1	260	268	PF00244	0.444
LIG_14-3-3_CanoR_1	419	427	PF00244	0.691
LIG_14-3-3_CanoR_1	473	477	PF00244	0.793
LIG_14-3-3_CanoR_1	514	519	PF00244	0.720
LIG_14-3-3_CanoR_1	724	728	PF00244	0.460
LIG_14-3-3_CanoR_1	974	978	PF00244	0.547
LIG_Actin_WH2_2	544	561	PF00022	0.389
LIG_APCC_ABBAyCdc20_2	225	231	PF00400	0.401
LIG_BIR_III_2	788	792	PF00653	0.408
LIG_BIR_III_4	810	814	PF00653	0.344
LIG_BRCT_BRCA1_1	500	504	PF00533	0.676
LIG_Clathr_ClatBox_1	636	640	PF01394	0.627
LIG_eIF4E_1	64	70	PF01652	0.344
LIG_FHA_1	143	149	PF00498	0.754
LIG_FHA_1	253	259	PF00498	0.401
LIG_FHA_1	380	386	PF00498	0.660
LIG_FHA_1	522	528	PF00498	0.701
LIG_FHA_1	575	581	PF00498	0.552
LIG_FHA_1	583	589	PF00498	0.483
LIG_FHA_1	788	794	PF00498	0.444
LIG_FHA_1	884	890	PF00498	0.408
LIG_FHA_2	189	195	PF00498	0.362
LIG_FHA_2	296	302	PF00498	0.344
LIG_FHA_2	36	42	PF00498	0.477
LIG_FHA_2	666	672	PF00498	0.410
LIG_FHA_2	951	957	PF00498	0.401
LIG_GBD_Chelix_1	170	178	PF00786	0.444
LIG_GBD_Chelix_1	331	339	PF00786	0.485
LIG_LIR_Apic_2	266	272	PF02991	0.344
LIG_LIR_Gen_1	501	512	PF02991	0.701
LIG_LIR_Gen_1	63	71	PF02991	0.344
LIG_LIR_Gen_1	751	760	PF02991	0.444
LIG_LIR_Gen_1	816	824	PF02991	0.444
LIG_LIR_Nem_3	240	245	PF02991	0.401
LIG_LIR_Nem_3	501	507	PF02991	0.706
LIG_LIR_Nem_3	63	67	PF02991	0.344
LIG_LIR_Nem_3	751	755	PF02991	0.444
LIG_LIR_Nem_3	816	820	PF02991	0.444
LIG_LRP6_Inhibitor_1	862	868	PF00058	0.344
LIG_LRP6_Inhibitor_1	908	914	PF00058	0.444
LIG_MAD2	51	59	PF02301	0.401
LIG_NRBOX	292	298	PF00104	0.444
LIG_NRBOX	330	336	PF00104	0.369
LIG_PCNA_yPIPBox_3	181	192	PF02747	0.446
LIG_Pex14_2	67	71	PF04695	0.344
LIG_PTB_Apo_2	221	228	PF02174	0.252
LIG_SH2_CRK	242	246	PF00017	0.485
LIG_SH2_CRK	817	821	PF00017	0.444
LIG_SH2_NCK_1	817	821	PF00017	0.444
LIG_SH2_STAP1	30	34	PF00017	0.444
LIG_SH2_STAP1	327	331	PF00017	0.485
LIG_SH2_STAT5	269	272	PF00017	0.344
LIG_SH2_STAT5	4	7	PF00017	0.289
LIG_SH2_STAT5	64	67	PF00017	0.344
LIG_SH2_STAT5	899	902	PF00017	0.327
LIG_SH3_3	261	267	PF00018	0.344
LIG_SH3_3	477	483	PF00018	0.715
LIG_SH3_3	656	662	PF00018	0.661
LIG_SH3_3	713	719	PF00018	0.441
LIG_SUMO_SIM_par_1	290	295	PF11976	0.344
LIG_SUMO_SIM_par_1	579	586	PF11976	0.463
LIG_SUMO_SIM_par_1	601	608	PF11976	0.479
LIG_TRAF2_1	191	194	PF00917	0.408
LIG_TRAF2_1	38	41	PF00917	0.416
LIG_TRFH_1	268	272	PF08558	0.401
LIG_UBA3_1	372	380	PF00899	0.711
MOD_CDK_SPK_2	787	792	PF00069	0.444
MOD_CDK_SPxxK_3	787	794	PF00069	0.444
MOD_CK1_1	142	148	PF00069	0.691
MOD_CK1_1	151	157	PF00069	0.721
MOD_CK1_1	26	32	PF00069	0.485
MOD_CK1_1	418	424	PF00069	0.745
MOD_CK1_1	469	475	PF00069	0.751
MOD_CK1_1	484	490	PF00069	0.736
MOD_CK1_1	519	525	PF00069	0.723
MOD_CK1_1	60	66	PF00069	0.444
MOD_CK1_1	660	666	PF00069	0.580
MOD_CK1_1	685	691	PF00069	0.344
MOD_CK1_1	692	698	PF00069	0.333
MOD_CK1_1	973	979	PF00069	0.585
MOD_CK2_1	107	113	PF00069	0.668
MOD_CK2_1	188	194	PF00069	0.425
MOD_CK2_1	199	205	PF00069	0.379
MOD_CK2_1	207	213	PF00069	0.262
MOD_CK2_1	295	301	PF00069	0.441
MOD_CK2_1	35	41	PF00069	0.477
MOD_CK2_1	665	671	PF00069	0.436
MOD_CK2_1	677	683	PF00069	0.342
MOD_CK2_1	860	866	PF00069	0.369
MOD_CK2_1	889	895	PF00069	0.419
MOD_GlcNHglycan	121	124	PF01048	0.702
MOD_GlcNHglycan	141	144	PF01048	0.471
MOD_GlcNHglycan	261	264	PF01048	0.444
MOD_GlcNHglycan	368	371	PF01048	0.729
MOD_GlcNHglycan	442	445	PF01048	0.740
MOD_GlcNHglycan	532	536	PF01048	0.742
MOD_GlcNHglycan	615	618	PF01048	0.461
MOD_GlcNHglycan	652	655	PF01048	0.781
MOD_GlcNHglycan	683	687	PF01048	0.339
MOD_GlcNHglycan	697	700	PF01048	0.356
MOD_GlcNHglycan	705	708	PF01048	0.364
MOD_GlcNHglycan	775	778	PF01048	0.405
MOD_GlcNHglycan	810	814	PF01048	0.344
MOD_GlcNHglycan	833	836	PF01048	0.444
MOD_GlcNHglycan	89	92	PF01048	0.778
MOD_GlcNHglycan	891	894	PF01048	0.405
MOD_GSK3_1	105	112	PF00069	0.700
MOD_GSK3_1	130	137	PF00069	0.814
MOD_GSK3_1	147	154	PF00069	0.640
MOD_GSK3_1	195	202	PF00069	0.408
MOD_GSK3_1	22	29	PF00069	0.477
MOD_GSK3_1	259	266	PF00069	0.344
MOD_GSK3_1	326	333	PF00069	0.331
MOD_GSK3_1	355	362	PF00069	0.524
MOD_GSK3_1	418	425	PF00069	0.705
MOD_GSK3_1	426	433	PF00069	0.659
MOD_GSK3_1	436	443	PF00069	0.587
MOD_GSK3_1	453	460	PF00069	0.745
MOD_GSK3_1	461	468	PF00069	0.768
MOD_GSK3_1	481	488	PF00069	0.460
MOD_GSK3_1	498	505	PF00069	0.609
MOD_GSK3_1	512	519	PF00069	0.668
MOD_GSK3_1	613	620	PF00069	0.440
MOD_GSK3_1	685	692	PF00069	0.464
MOD_GSK3_1	705	712	PF00069	0.462
MOD_N-GLC_1	425	430	PF02516	0.804
MOD_N-GLC_1	466	471	PF02516	0.754
MOD_N-GLC_1	485	490	PF02516	0.648
MOD_N-GLC_1	574	579	PF02516	0.625
MOD_NEK2_1	105	110	PF00069	0.653
MOD_NEK2_1	195	200	PF00069	0.474
MOD_NEK2_1	252	257	PF00069	0.369
MOD_NEK2_1	325	330	PF00069	0.344
MOD_NEK2_1	368	373	PF00069	0.733
MOD_NEK2_1	397	402	PF00069	0.654
MOD_NEK2_1	457	462	PF00069	0.781
MOD_NEK2_1	521	526	PF00069	0.602
MOD_NEK2_1	582	587	PF00069	0.556
MOD_NEK2_1	763	768	PF00069	0.444
MOD_NEK2_1	876	881	PF00069	0.344
MOD_NEK2_1	921	926	PF00069	0.348
MOD_PIKK_1	521	527	PF00454	0.691
MOD_PK_1	355	361	PF00069	0.624
MOD_PK_1	57	63	PF00069	0.444
MOD_PKA_1	657	663	PF00069	0.599
MOD_PKA_2	188	194	PF00069	0.386
MOD_PKA_2	23	29	PF00069	0.485
MOD_PKA_2	259	265	PF00069	0.344
MOD_PKA_2	418	424	PF00069	0.693
MOD_PKA_2	472	478	PF00069	0.794
MOD_PKA_2	617	623	PF00069	0.401
MOD_PKA_2	660	666	PF00069	0.580
MOD_PKA_2	677	683	PF00069	0.344
MOD_PKA_2	710	716	PF00069	0.431
MOD_PKA_2	723	729	PF00069	0.460
MOD_PKA_2	973	979	PF00069	0.546
MOD_PKB_1	413	421	PF00069	0.723
MOD_Plk_1	2	8	PF00069	0.289
MOD_Plk_1	425	431	PF00069	0.728
MOD_Plk_1	519	525	PF00069	0.723
MOD_Plk_2-3	295	301	PF00069	0.344
MOD_Plk_2-3	739	745	PF00069	0.408
MOD_Plk_4	100	106	PF00069	0.750
MOD_Plk_4	148	154	PF00069	0.772
MOD_Plk_4	207	213	PF00069	0.344
MOD_Plk_4	303	309	PF00069	0.405
MOD_Plk_4	314	320	PF00069	0.317
MOD_Plk_4	330	336	PF00069	0.442
MOD_Plk_4	348	354	PF00069	0.293
MOD_Plk_4	368	374	PF00069	0.709
MOD_Plk_4	426	432	PF00069	0.722
MOD_Plk_4	540	546	PF00069	0.531
MOD_Plk_4	57	63	PF00069	0.355
MOD_Plk_4	717	723	PF00069	0.466
MOD_Plk_4	748	754	PF00069	0.401
MOD_Plk_4	973	979	PF00069	0.516
MOD_ProDKin_1	263	269	PF00069	0.344
MOD_ProDKin_1	345	351	PF00069	0.343
MOD_ProDKin_1	360	366	PF00069	0.582
MOD_ProDKin_1	442	448	PF00069	0.754
MOD_ProDKin_1	461	467	PF00069	0.573
MOD_ProDKin_1	595	601	PF00069	0.473
MOD_ProDKin_1	605	611	PF00069	0.566
MOD_ProDKin_1	692	698	PF00069	0.291
MOD_ProDKin_1	715	721	PF00069	0.485
MOD_ProDKin_1	787	793	PF00069	0.444
MOD_SUMO_rev_2	240	245	PF00179	0.344
TRG_DiLeu_BaEn_1	49	54	PF01217	0.344
TRG_ENDOCYTIC_2	242	245	PF00928	0.401
TRG_ENDOCYTIC_2	64	67	PF00928	0.344
TRG_ENDOCYTIC_2	817	820	PF00928	0.444
TRG_ENDOCYTIC_2	941	944	PF00928	0.344
TRG_ER_diArg_1	76	78	PF00400	0.408
TRG_ER_diArg_1	791	794	PF00400	0.444
TRG_NES_CRM1_1	285	295	PF08389	0.478
TRG_NLS_MonoExtC_3	6	12	PF00514	0.444
TRG_NLS_MonoExtN_4	837	844	PF00514	0.444
TRG_Pf-PMV_PEXEL_1	274	278	PF00026	0.344
TRG_Pf-PMV_PEXEL_1	491	496	PF00026	0.755
TRG_Pf-PMV_PEXEL_1	767	771	PF00026	0.401
TRG_Pf-PMV_PEXEL_1	8	12	PF00026	0.444

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PFF2	Leptomonas seymouri	72%	99%
A0A1X0P1U2	Trypanosomatidae	46%	100%
A0A3Q8IEB1	Leishmania donovani	95%	99%
A0A422N709	Trypanosoma rangeli	47%	100%
A4HIE5	Leishmania braziliensis	84%	100%
A4I5P2	Leishmania infantum	95%	99%
E9B0Y6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	94%	99%
V5DCX1	Trypanosoma cruzi	47%	100%