Topoisomerase I subunit B

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

DNA topoisomerase type IB small subunit, putative

Species:

Leishmania major

UniProt:

Q95Z89_LEIMA

TriTrypDb:

LmjF.04.0060 , LMJLV39_040005700 , LMJSD75_040005700

Length:

262

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 6
NetGPI	no	yes: 0, no: 6

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	2
GO:0005694	chromosome	5	7
GO:0005730	nucleolus	5	2
GO:0043226	organelle	2	7
GO:0043227	membrane-bounded organelle	3	2
GO:0043228	non-membrane-bounded organelle	3	7
GO:0043229	intracellular organelle	3	7
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0043232	intracellular non-membrane-bounded organelle	4	7
GO:0110165	cellular anatomical entity	1	7

Expansion

Sequence features

Q95Z89

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q95Z89

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	7
GO:0006259	DNA metabolic process	4	7
GO:0006260	DNA replication	5	2
GO:0006265	DNA topological change	5	7
GO:0006725	cellular aromatic compound metabolic process	3	7
GO:0006807	nitrogen compound metabolic process	2	7
GO:0006996	organelle organization	4	7
GO:0007059	chromosome segregation	2	2
GO:0008152	metabolic process	1	7
GO:0009987	cellular process	1	7
GO:0016043	cellular component organization	3	7
GO:0034641	cellular nitrogen compound metabolic process	3	7
GO:0043170	macromolecule metabolic process	3	7
GO:0044237	cellular metabolic process	2	7
GO:0044238	primary metabolic process	2	7
GO:0044260	obsolete cellular macromolecule metabolic process	3	7
GO:0046483	heterocycle metabolic process	3	7
GO:0051276	chromosome organization	5	7
GO:0071103	DNA conformation change	6	7
GO:0071704	organic substance metabolic process	2	7
GO:0071840	cellular component organization or biogenesis	2	7
GO:0090304	nucleic acid metabolic process	4	7
GO:1901360	organic cyclic compound metabolic process	3	7

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	7
GO:0003677	DNA binding	4	7
GO:0003824	catalytic activity	1	7
GO:0003916	DNA topoisomerase activity	3	7
GO:0003917	DNA topoisomerase type I (single strand cut, ATP-independent) activity	4	7
GO:0005488	binding	1	7
GO:0016853	isomerase activity	2	7
GO:0097159	organic cyclic compound binding	2	7
GO:0140097	catalytic activity, acting on DNA	3	7
GO:0140640	catalytic activity, acting on a nucleic acid	2	7
GO:1901363	heterocyclic compound binding	2	7

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	117	121	PF00656	0.602
CLV_NRD_NRD_1	92	94	PF00675	0.625
CLV_PCSK_KEX2_1	92	94	PF00082	0.625
CLV_PCSK_SKI1_1	158	162	PF00082	0.538
CLV_PCSK_SKI1_1	66	70	PF00082	0.653
DEG_COP1_1	162	171	PF00400	0.574
DEG_Nend_UBRbox_3	1	3	PF02207	0.608
DEG_SPOP_SBC_1	94	98	PF00917	0.634
DOC_CYCLIN_yCln2_LP_2	171	174	PF00134	0.592
DOC_MAPK_gen_1	196	206	PF00069	0.369
DOC_MAPK_gen_1	20	26	PF00069	0.510
DOC_PP2B_LxvP_1	171	174	PF13499	0.592
DOC_PP4_MxPP_1	71	74	PF00568	0.539
DOC_USP7_UBL2_3	207	211	PF12436	0.304
DOC_WW_Pin1_4	19	24	PF00397	0.563
DOC_WW_Pin1_4	3	8	PF00397	0.705
DOC_WW_Pin1_4	31	36	PF00397	0.524
LIG_14-3-3_CanoR_1	131	140	PF00244	0.590
LIG_14-3-3_CanoR_1	226	232	PF00244	0.369
LIG_14-3-3_CanoR_1	38	42	PF00244	0.550
LIG_FHA_1	215	221	PF00498	0.304
LIG_FHA_2	160	166	PF00498	0.672
LIG_FHA_2	20	26	PF00498	0.595
LIG_MYND_1	169	173	PF01753	0.604
LIG_PDZ_Class_2	257	262	PF00595	0.304
LIG_SH2_PTP2	222	225	PF00017	0.304
LIG_SH2_SRC	222	225	PF00017	0.304
LIG_SH2_STAT5	222	225	PF00017	0.304
LIG_SH3_3	170	176	PF00018	0.528
LIG_SH3_3	5	11	PF00018	0.627
LIG_SUMO_SIM_par_1	22	27	PF11976	0.543
LIG_TRAF2_1	162	165	PF00917	0.577
LIG_UBA3_1	203	211	PF00899	0.284
LIG_UBA3_1	62	69	PF00899	0.590
LIG_WW_3	172	176	PF00397	0.516
MOD_CDK_SPxxK_3	31	38	PF00069	0.531
MOD_CK1_1	104	110	PF00069	0.620
MOD_CK1_1	118	124	PF00069	0.690
MOD_CK1_1	125	131	PF00069	0.654
MOD_CK1_1	132	138	PF00069	0.711
MOD_CK1_1	214	220	PF00069	0.304
MOD_CK1_1	96	102	PF00069	0.670
MOD_CK2_1	131	137	PF00069	0.607
MOD_CK2_1	159	165	PF00069	0.516
MOD_GlcNHglycan	101	104	PF01048	0.592
MOD_GlcNHglycan	134	137	PF01048	0.592
MOD_GlcNHglycan	140	143	PF01048	0.548
MOD_GlcNHglycan	243	246	PF01048	0.369
MOD_GlcNHglycan	25	29	PF01048	0.504
MOD_GSK3_1	101	108	PF00069	0.622
MOD_GSK3_1	111	118	PF00069	0.619
MOD_GSK3_1	120	127	PF00069	0.684
MOD_GSK3_1	131	138	PF00069	0.673
MOD_GSK3_1	211	218	PF00069	0.304
MOD_GSK3_1	33	40	PF00069	0.492
MOD_GSK3_1	93	100	PF00069	0.679
MOD_NEK2_1	215	220	PF00069	0.304
MOD_NEK2_1	243	248	PF00069	0.304
MOD_NEK2_2	142	147	PF00069	0.514
MOD_PIKK_1	149	155	PF00454	0.457
MOD_PIKK_1	159	165	PF00454	0.548
MOD_PKA_2	130	136	PF00069	0.594
MOD_PKA_2	37	43	PF00069	0.639
MOD_Plk_1	121	127	PF00069	0.661
MOD_Plk_1	142	148	PF00069	0.473
MOD_Plk_1	24	30	PF00069	0.524
MOD_Plk_2-3	116	122	PF00069	0.641
MOD_Plk_4	135	141	PF00069	0.629
MOD_Plk_4	142	148	PF00069	0.467
MOD_Plk_4	167	173	PF00069	0.609
MOD_ProDKin_1	19	25	PF00069	0.559
MOD_ProDKin_1	3	9	PF00069	0.704
MOD_ProDKin_1	31	37	PF00069	0.519
MOD_SUMO_rev_2	208	213	PF00179	0.304
TRG_DiLeu_BaEn_1	167	172	PF01217	0.571
TRG_ER_diArg_1	91	93	PF00400	0.628

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IID6	Leptomonas seymouri	63%	100%
A0A451EJV3	Leishmania donovani	96%	100%
A4H3S7	Leishmania braziliensis	80%	97%
A4HS02	Leishmania infantum	95%	100%
E9AJZ0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	95%	100%