Ribonuclease

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Ribonuclease

Gene product:

ribonuclease H, putative

Species:

Leishmania major

UniProt:

Q8ISS8_LEIMA

TriTrypDb:

LmjF.36.0640 , LMJLV39_360012100 , LMJSD75_360012000

Length:

301

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	2
GO:0032299	ribonuclease H2 complex	3	2
GO:0032991	protein-containing complex	1	2
GO:0110165	cellular anatomical entity	1	2
GO:0140535	intracellular protein-containing complex	2	2
GO:1902494	catalytic complex	2	2

Expansion

Sequence features

Q8ISS8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q8ISS8

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	2
GO:0006259	DNA metabolic process	4	2
GO:0006281	DNA repair	5	2
GO:0006298	mismatch repair	6	2
GO:0006401	RNA catabolic process	5	2
GO:0006725	cellular aromatic compound metabolic process	3	2
GO:0006807	nitrogen compound metabolic process	2	2
GO:0006950	response to stress	2	2
GO:0006974	DNA damage response	4	2
GO:0008152	metabolic process	1	2
GO:0009056	catabolic process	2	2
GO:0009057	macromolecule catabolic process	4	2
GO:0009987	cellular process	1	2
GO:0016070	RNA metabolic process	5	2
GO:0019439	aromatic compound catabolic process	4	2
GO:0033554	cellular response to stress	3	2
GO:0034641	cellular nitrogen compound metabolic process	3	2
GO:0034655	nucleobase-containing compound catabolic process	4	2
GO:0043137	DNA replication, removal of RNA primer	6	2
GO:0043170	macromolecule metabolic process	3	2
GO:0044237	cellular metabolic process	2	2
GO:0044238	primary metabolic process	2	2
GO:0044248	cellular catabolic process	3	2
GO:0044260	obsolete cellular macromolecule metabolic process	3	2
GO:0044265	obsolete cellular macromolecule catabolic process	4	2
GO:0044270	cellular nitrogen compound catabolic process	4	2
GO:0046483	heterocycle metabolic process	3	2
GO:0046700	heterocycle catabolic process	4	2
GO:0050896	response to stimulus	1	2
GO:0051716	cellular response to stimulus	2	2
GO:0071704	organic substance metabolic process	2	2
GO:0090304	nucleic acid metabolic process	4	2
GO:1901360	organic cyclic compound metabolic process	3	2
GO:1901361	organic cyclic compound catabolic process	4	2
GO:1901575	organic substance catabolic process	3	2

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	11
GO:0003723	RNA binding	4	11
GO:0003824	catalytic activity	1	11
GO:0004518	nuclease activity	4	11
GO:0004519	endonuclease activity	5	11
GO:0004521	RNA endonuclease activity	5	11
GO:0004523	RNA-DNA hybrid ribonuclease activity	7	11
GO:0004540	RNA nuclease activity	4	11
GO:0005488	binding	1	11
GO:0016787	hydrolase activity	2	11
GO:0016788	hydrolase activity, acting on ester bonds	3	11
GO:0016891	RNA endonuclease activity, producing 5'-phosphomonoesters	6	11
GO:0016893	endonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters	6	11
GO:0043167	ion binding	2	11
GO:0043169	cation binding	3	11
GO:0046872	metal ion binding	4	11
GO:0097159	organic cyclic compound binding	2	11
GO:0140098	catalytic activity, acting on RNA	3	11
GO:0140640	catalytic activity, acting on a nucleic acid	2	11
GO:1901363	heterocyclic compound binding	2	11

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	92	94	PF00675	0.248
CLV_PCSK_KEX2_1	20	22	PF00082	0.617
CLV_PCSK_KEX2_1	297	299	PF00082	0.581
CLV_PCSK_KEX2_1	91	93	PF00082	0.274
CLV_PCSK_PC1ET2_1	20	22	PF00082	0.600
CLV_PCSK_PC1ET2_1	297	299	PF00082	0.693
CLV_PCSK_SKI1_1	101	105	PF00082	0.270
CLV_PCSK_SKI1_1	107	111	PF00082	0.270
CLV_PCSK_SKI1_1	120	124	PF00082	0.237
DEG_APCC_DBOX_1	90	98	PF00400	0.558
DOC_ANK_TNKS_1	53	60	PF00023	0.506
DOC_MAPK_FxFP_2	237	240	PF00069	0.474
DOC_MAPK_gen_1	104	113	PF00069	0.473
DOC_MAPK_gen_1	20	29	PF00069	0.541
DOC_MAPK_MEF2A_6	174	182	PF00069	0.454
DOC_MAPK_MEF2A_6	247	256	PF00069	0.541
DOC_MAPK_RevD_3	77	92	PF00069	0.474
DOC_PP1_RVXF_1	286	292	PF00149	0.560
DOC_PP4_FxxP_1	237	240	PF00568	0.441
DOC_PP4_FxxP_1	291	294	PF00568	0.542
DOC_USP7_MATH_1	191	195	PF00917	0.465
DOC_USP7_MATH_1	22	26	PF00917	0.627
DOC_USP7_MATH_1	246	250	PF00917	0.499
DOC_USP7_UBL2_3	293	297	PF12436	0.682
DOC_WW_Pin1_4	1	6	PF00397	0.696
DOC_WW_Pin1_4	111	116	PF00397	0.447
DOC_WW_Pin1_4	292	297	PF00397	0.473
LIG_14-3-3_CanoR_1	101	107	PF00244	0.426
LIG_14-3-3_CanoR_1	128	134	PF00244	0.530
LIG_14-3-3_CanoR_1	179	183	PF00244	0.454
LIG_14-3-3_CanoR_1	235	240	PF00244	0.478
LIG_14-3-3_CanoR_1	247	251	PF00244	0.411
LIG_14-3-3_CanoR_1	92	98	PF00244	0.494
LIG_DLG_GKlike_1	93	100	PF00625	0.534
LIG_EH1_1	28	36	PF00400	0.572
LIG_FHA_1	108	114	PF00498	0.458
LIG_FHA_1	62	68	PF00498	0.534
LIG_FHA_2	200	206	PF00498	0.534
LIG_LIR_Apic_2	290	294	PF02991	0.540
LIG_LIR_Gen_1	41	52	PF02991	0.467
LIG_LIR_Nem_3	105	109	PF02991	0.454
LIG_LIR_Nem_3	187	193	PF02991	0.526
LIG_LIR_Nem_3	249	253	PF02991	0.491
LIG_LIR_Nem_3	41	47	PF02991	0.479
LIG_SH2_STAT5	157	160	PF00017	0.443
LIG_SH2_STAT5	44	47	PF00017	0.449
LIG_SH2_STAT5	63	66	PF00017	0.322
LIG_SH3_3	158	164	PF00018	0.427
LIG_SH3_3	217	223	PF00018	0.447
LIG_SH3_3	259	265	PF00018	0.497
LIG_SH3_3	54	60	PF00018	0.468
LIG_SH3_4	293	300	PF00018	0.687
LIG_SUMO_SIM_par_1	66	72	PF11976	0.454
LIG_TRAF2_1	114	117	PF00917	0.534
LIG_Vh1_VBS_1	192	210	PF01044	0.335
LIG_WRC_WIRS_1	103	108	PF05994	0.291
MOD_CDC14_SPxK_1	295	298	PF00782	0.486
MOD_CDK_SPK_2	292	297	PF00069	0.473
MOD_CDK_SPxK_1	292	298	PF00069	0.477
MOD_CK1_1	134	140	PF00069	0.294
MOD_CK1_1	143	149	PF00069	0.280
MOD_CK1_1	194	200	PF00069	0.419
MOD_CK1_1	6	12	PF00069	0.576
MOD_CK2_1	111	117	PF00069	0.302
MOD_CK2_1	203	209	PF00069	0.289
MOD_CK2_1	22	28	PF00069	0.569
MOD_CK2_1	66	72	PF00069	0.419
MOD_GlcNHglycan	125	128	PF01048	0.413
MOD_GlcNHglycan	148	151	PF01048	0.357
MOD_GlcNHglycan	16	19	PF01048	0.552
MOD_GlcNHglycan	193	196	PF01048	0.419
MOD_GlcNHglycan	216	219	PF01048	0.270
MOD_GlcNHglycan	5	8	PF01048	0.589
MOD_GSK3_1	107	114	PF00069	0.307
MOD_GSK3_1	116	123	PF00069	0.307
MOD_GSK3_1	127	134	PF00069	0.122
MOD_GSK3_1	199	206	PF00069	0.347
MOD_GSK3_1	214	221	PF00069	0.201
MOD_GSK3_1	34	41	PF00069	0.394
MOD_GSK3_1	6	13	PF00069	0.585
MOD_NEK2_1	16	21	PF00069	0.529
MOD_NEK2_1	178	183	PF00069	0.307
MOD_NEK2_1	61	66	PF00069	0.267
MOD_PK_1	185	191	PF00069	0.219
MOD_PKA_2	10	16	PF00069	0.645
MOD_PKA_2	127	133	PF00069	0.219
MOD_PKA_2	178	184	PF00069	0.334
MOD_PKA_2	203	209	PF00069	0.316
MOD_PKA_2	22	28	PF00069	0.566
MOD_PKA_2	246	252	PF00069	0.307
MOD_PKB_1	91	99	PF00069	0.419
MOD_Plk_1	143	149	PF00069	0.413
MOD_Plk_2-3	222	228	PF00069	0.335
MOD_Plk_4	185	191	PF00069	0.314
MOD_Plk_4	194	200	PF00069	0.278
MOD_Plk_4	22	28	PF00069	0.691
MOD_ProDKin_1	1	7	PF00069	0.695
MOD_ProDKin_1	111	117	PF00069	0.297
MOD_ProDKin_1	292	298	PF00069	0.477
TRG_DiLeu_BaEn_1	72	77	PF01217	0.307
TRG_ENDOCYTIC_2	44	47	PF00928	0.470
TRG_ER_diArg_1	91	93	PF00400	0.419
TRG_Pf-PMV_PEXEL_1	86	90	PF00026	0.297

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P7W5	Leptomonas seymouri	80%	95%
A0A1X0P866	Trypanosomatidae	59%	91%
A0A3R7RS79	Trypanosoma rangeli	61%	93%
A0A3S7XAC0	Leishmania donovani	86%	95%
A0B796	Methanothrix thermoacetophila (strain DSM 6194 / JCM 14653 / NBRC 101360 / PT)	32%	100%
A0RV25	Cenarchaeum symbiosum (strain A)	32%	100%
A2BL34	Hyperthermus butylicus (strain DSM 5456 / JCM 9403 / PLM1-5)	40%	100%
A2STJ6	Methanocorpusculum labreanum (strain ATCC 43576 / DSM 4855 / Z)	33%	100%
A4FWE5	Methanococcus maripaludis (strain C5 / ATCC BAA-1333)	32%	100%
A4HNS4	Leishmania braziliensis	86%	95%
A4ICS0	Leishmania infantum	88%	95%
A5ULV6	Methanobrevibacter smithii (strain ATCC 35061 / DSM 861 / OCM 144 / PS)	34%	100%
A6UQ18	Methanococcus vannielii (strain ATCC 35089 / DSM 1224 / JCM 13029 / OCM 148 / SB)	34%	100%
A6UUM1	Methanococcus aeolicus (strain ATCC BAA-1280 / DSM 17508 / OCM 812 / Nankai-3)	28%	100%
A6VGW0	Methanococcus maripaludis (strain C7 / ATCC BAA-1331)	31%	100%
A7I9P7	Methanoregula boonei (strain DSM 21154 / JCM 14090 / 6A8)	35%	100%
A8MAH5	Caldivirga maquilingensis (strain ATCC 700844 / DSM 13496 / JCM 10307 / IC-167)	37%	100%
A9A9T9	Methanococcus maripaludis (strain C6 / ATCC BAA-1332)	31%	100%
B0R6T4	Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1)	33%	100%
B1YS60	Burkholderia ambifaria (strain MC40-6)	28%	100%
B6YVT5	Thermococcus onnurineus (strain NA1)	35%	100%
B8GQ66	Thioalkalivibrio sulfidiphilus (strain HL-EbGR7)	30%	100%
C6A1I4	Thermococcus sibiricus (strain DSM 12597 / MM 739)	35%	100%
D0A2N4	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	60%	92%
E9ASI4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	83%	95%
O27102	Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H)	39%	100%
O59351	Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)	34%	100%
O74035	Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1)	37%	100%
O75792	Homo sapiens	46%	100%
P53942	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	37%	98%
Q0BE29	Burkholderia ambifaria (strain ATCC BAA-244 / AMMD)	28%	100%
Q0W5R8	Methanocella arvoryzae (strain DSM 22066 / NBRC 105507 / MRE50)	33%	100%
Q10236	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	43%	92%
Q12YN9	Methanococcoides burtonii (strain DSM 6242 / NBRC 107633 / OCM 468 / ACE-M)	36%	100%
Q2FP80	Methanospirillum hungatei JF-1 (strain ATCC 27890 / DSM 864 / NBRC 100397 / JF-1)	35%	100%
Q2NEI6	Methanosphaera stadtmanae (strain ATCC 43021 / DSM 3091 / JCM 11832 / MCB-3)	31%	100%
Q2TBT5	Bos taurus	47%	100%
Q3IRF6	Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)	33%	100%
Q468N6	Methanosarcina barkeri (strain Fusaro / DSM 804)	31%	100%
Q4JA60	Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)	32%	100%
Q54D13	Dictyostelium discoideum	34%	100%
Q57599	Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)	30%	100%
Q5U209	Rattus norvegicus	47%	100%
Q5UXT4	Haloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809)	32%	100%
Q6LXH7	Methanococcus maripaludis (strain S2 / LL)	32%	100%
Q8PTA3	Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88)	32%	100%
Q8TPF4	Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)	31%	100%
Q8U036	Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)	36%	100%
Q974Z3	Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)	33%	100%
Q97CG6	Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)	35%	100%
Q97W56	Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)	37%	100%
Q9CWY8	Mus musculus	48%	100%
Q9HNR3	Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1)	33%	100%
Q9P5X8	Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)	36%	95%
Q9SEZ6	Arabidopsis thaliana	41%	100%
Q9U6P6	Caenorhabditis elegans	39%	100%
Q9V1A9	Pyrococcus abyssi (strain GE5 / Orsay)	36%	100%
Q9VPP5	Drosophila melanogaster	39%	87%
Q9YET5	Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)	34%	100%
V5BQG7	Trypanosoma cruzi	58%	93%