Lactoylglutathione lyase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

trypanothione-dependent glyoxalase I

Species:

Leishmania major

UniProt:

Q68RJ8_LEIMA

TriTrypDb:

LmjF.35.3010 , LMJLV39_350037400 , LMJSD75_350036800

Length:

141

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 9

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

Q68RJ8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q68RJ8

PDB structure(s): 2c21_A , 2c21_B , 2c21_C , 2c21_D , 2c21_E , 2c21_F

Function

Biological processes
Term	Name	Level	Count
GO:0006081	cellular aldehyde metabolic process	3	2
GO:0006082	organic acid metabolic process	3	2
GO:0006089	lactate metabolic process	4	2
GO:0008152	metabolic process	1	2
GO:0009056	catabolic process	2	2
GO:0009438	methylglyoxal metabolic process	4	2
GO:0009987	cellular process	1	2
GO:0019243	methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione	6	2
GO:0019752	carboxylic acid metabolic process	5	2
GO:0032787	monocarboxylic acid metabolic process	6	2
GO:0042180	cellular ketone metabolic process	3	2
GO:0042182	ketone catabolic process	4	2
GO:0043436	oxoacid metabolic process	4	2
GO:0044237	cellular metabolic process	2	2
GO:0044248	cellular catabolic process	3	2
GO:0044281	small molecule metabolic process	2	2
GO:0044282	small molecule catabolic process	3	2
GO:0046185	aldehyde catabolic process	4	2
GO:0051596	methylglyoxal catabolic process	5	2
GO:0061727	methylglyoxal catabolic process to lactate	5	2
GO:0071704	organic substance metabolic process	2	2
GO:1901575	organic substance catabolic process	3	2
GO:1901615	organic hydroxy compound metabolic process	3	2

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	11
GO:0004462	lactoylglutathione lyase activity	4	11
GO:0005488	binding	1	11
GO:0016829	lyase activity	2	11
GO:0016846	carbon-sulfur lyase activity	3	11
GO:0043167	ion binding	2	11
GO:0043169	cation binding	3	11
GO:0046872	metal ion binding	4	11

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	32	34	PF00675	0.324
CLV_NRD_NRD_1	4	6	PF00675	0.431
CLV_NRD_NRD_1	92	94	PF00675	0.386
CLV_PCSK_KEX2_1	4	6	PF00082	0.431
CLV_PCSK_SKI1_1	18	22	PF00082	0.330
CLV_PCSK_SKI1_1	4	8	PF00082	0.357
DEG_APCC_DBOX_1	3	11	PF00400	0.362
DOC_MAPK_gen_1	93	99	PF00069	0.315
DOC_PP1_RVXF_1	16	23	PF00149	0.304
DOC_USP7_MATH_1	51	55	PF00917	0.451
DOC_USP7_UBL2_3	30	34	PF12436	0.416
LIG_14-3-3_CanoR_1	5	11	PF00244	0.297
LIG_BRCT_BRCA1_1	18	22	PF00533	0.304
LIG_FHA_1	22	28	PF00498	0.304
LIG_FHA_2	124	130	PF00498	0.326
LIG_FHA_2	54	60	PF00498	0.345
LIG_KLC1_Yacidic_2	115	120	PF13176	0.304
LIG_LIR_Gen_1	104	114	PF02991	0.305
LIG_LIR_Gen_1	115	122	PF02991	0.302
LIG_LIR_Gen_1	39	49	PF02991	0.338
LIG_LIR_Nem_3	104	110	PF02991	0.305
LIG_LIR_Nem_3	115	121	PF02991	0.302
LIG_LIR_Nem_3	39	45	PF02991	0.316
LIG_LIR_Nem_3	72	78	PF02991	0.419
LIG_MAD2	30	38	PF02301	0.386
LIG_SH2_CRK	42	46	PF00017	0.448
LIG_SH2_NCK_1	42	46	PF00017	0.416
LIG_SH2_PTP2	118	121	PF00017	0.304
LIG_SH2_STAP1	23	27	PF00017	0.304
LIG_SH2_STAP1	42	46	PF00017	0.304
LIG_SH2_STAT5	118	121	PF00017	0.304
LIG_SH2_STAT5	23	26	PF00017	0.304
LIG_SH2_STAT5	42	45	PF00017	0.231
LIG_SH3_3	108	114	PF00018	0.416
LIG_SUMO_SIM_par_1	54	61	PF11976	0.318
MOD_CK2_1	123	129	PF00069	0.317
MOD_CK2_1	53	59	PF00069	0.331
MOD_GlcNHglycan	102	107	PF01048	0.401
MOD_NEK2_1	16	21	PF00069	0.268
MOD_NEK2_1	53	58	PF00069	0.309
MOD_NEK2_1	6	11	PF00069	0.308
MOD_Plk_1	123	129	PF00069	0.317
MOD_Plk_1	16	22	PF00069	0.304
MOD_Plk_1	58	64	PF00069	0.304
MOD_Plk_4	123	129	PF00069	0.446
MOD_Plk_4	40	46	PF00069	0.448
MOD_Plk_4	53	59	PF00069	0.119
MOD_Plk_4	6	12	PF00069	0.324
MOD_SUMO_rev_2	36	43	PF00179	0.210
MOD_SUMO_rev_2	83	88	PF00179	0.416
TRG_ENDOCYTIC_2	118	121	PF00928	0.289
TRG_ENDOCYTIC_2	42	45	PF00928	0.448
TRG_ENDOCYTIC_2	75	78	PF00928	0.288
TRG_ER_diArg_1	4	6	PF00400	0.433

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P4M0	Leptomonas seymouri	77%	100%
A0A0S4KKP2	Bodo saltans	64%	100%
A0A1X0P5K6	Trypanosomatidae	73%	100%
A0A3S7X9J4	Leishmania donovani	98%	100%
A0A422P2U3	Trypanosoma rangeli	74%	100%
A4HMW8	Leishmania braziliensis	93%	100%
A4IBI9	Leishmania infantum	98%	100%
E9B6I0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	97%	100%
O04885	Brassica juncea	33%	76%
O49818	Cicer arietinum	32%	76%
P0A0T2	Neisseria meningitidis serogroup A / serotype 4A (strain DSM 15465 / Z2491)	46%	100%
P0A0T3	Neisseria meningitidis serogroup B (strain MC58)	46%	100%
P0A1Q2	Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)	52%	100%
P0A1Q3	Salmonella typhi	52%	100%
P0AC81	Escherichia coli (strain K12)	52%	100%
P0AC82	Escherichia coli O157:H7	52%	100%
P0AC83	Shigella flexneri	52%	100%
P16635	Pseudomonas putida	32%	82%
P44638	Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)	51%	100%
P46235	Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)	48%	100%
Q04760	Homo sapiens	34%	77%
Q42891	Solanum lycopersicum	31%	76%
Q4R5F2	Macaca fascicularis	34%	77%
Q55595	Synechocystis sp. (strain PCC 6803 / Kazusa)	45%	100%
Q6P7Q4	Rattus norvegicus	34%	77%
Q8H0V3	Arabidopsis thaliana	31%	76%
Q9CPU0	Mus musculus	34%	77%
Q9HU72	Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)	33%	80%
Q9KT93	Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)	50%	100%
Q9ZS21	Glycine max	31%	76%
V5DUX3	Trypanosoma cruzi	72%	100%