Putative 3'-nucleotidase/nuclease

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

3'-nucleotidase/nuclease, putative

Species:

Leishmania major

UniProt:

Q66VY6_LEIMA

TriTrypDb:

LmjF.31.2300 , LMJLV39_310032400 , LMJSD75_310032400

Length:

382

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 15

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 7

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 7

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 22, no: 6
NetGPI	no	yes: 0, no: 28

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	11
GO:0110165	cellular anatomical entity	1	11
GO:0009986	cell surface	2	1

Expansion

Sequence features

Q66VY6

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q66VY6

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	29
GO:0006259	DNA metabolic process	4	29
GO:0006308	DNA catabolic process	5	29
GO:0006725	cellular aromatic compound metabolic process	3	29
GO:0006807	nitrogen compound metabolic process	2	29
GO:0008152	metabolic process	1	29
GO:0009056	catabolic process	2	29
GO:0009057	macromolecule catabolic process	4	29
GO:0009987	cellular process	1	29
GO:0019439	aromatic compound catabolic process	4	29
GO:0034641	cellular nitrogen compound metabolic process	3	29
GO:0034655	nucleobase-containing compound catabolic process	4	29
GO:0043170	macromolecule metabolic process	3	29
GO:0044237	cellular metabolic process	2	29
GO:0044238	primary metabolic process	2	29
GO:0044248	cellular catabolic process	3	29
GO:0044260	obsolete cellular macromolecule metabolic process	3	29
GO:0044265	obsolete cellular macromolecule catabolic process	4	29
GO:0044270	cellular nitrogen compound catabolic process	4	29
GO:0046483	heterocycle metabolic process	3	29
GO:0046700	heterocycle catabolic process	4	29
GO:0071704	organic substance metabolic process	2	29
GO:0090304	nucleic acid metabolic process	4	29
GO:1901360	organic cyclic compound metabolic process	3	29
GO:1901361	organic cyclic compound catabolic process	4	29
GO:1901575	organic substance catabolic process	3	29
GO:0006163	purine nucleotide metabolic process	5	1
GO:0006195	purine nucleotide catabolic process	6	1
GO:0006196	AMP catabolic process	7	1
GO:0006753	nucleoside phosphate metabolic process	4	1
GO:0006793	phosphorus metabolic process	3	1
GO:0006796	phosphate-containing compound metabolic process	4	1
GO:0009117	nucleotide metabolic process	5	1
GO:0009123	nucleoside monophosphate metabolic process	5	1
GO:0009125	nucleoside monophosphate catabolic process	6	1
GO:0009126	purine nucleoside monophosphate metabolic process	6	1
GO:0009128	purine nucleoside monophosphate catabolic process	7	1
GO:0009150	purine ribonucleotide metabolic process	6	1
GO:0009154	purine ribonucleotide catabolic process	6	1
GO:0009158	ribonucleoside monophosphate catabolic process	7	1
GO:0009161	ribonucleoside monophosphate metabolic process	6	1
GO:0009166	nucleotide catabolic process	6	1
GO:0009167	purine ribonucleoside monophosphate metabolic process	7	1
GO:0009169	purine ribonucleoside monophosphate catabolic process	8	1
GO:0009259	ribonucleotide metabolic process	5	1
GO:0009261	ribonucleotide catabolic process	5	1
GO:0019637	organophosphate metabolic process	3	1
GO:0019693	ribose phosphate metabolic process	4	1
GO:0044281	small molecule metabolic process	2	1
GO:0046033	AMP metabolic process	7	1
GO:0046434	organophosphate catabolic process	4	1
GO:0055086	nucleobase-containing small molecule metabolic process	3	1
GO:0072521	purine-containing compound metabolic process	4	1
GO:0072523	purine-containing compound catabolic process	5	1
GO:1901135	carbohydrate derivative metabolic process	3	1
GO:1901136	carbohydrate derivative catabolic process	4	1
GO:1901292	nucleoside phosphate catabolic process	5	1
GO:1901564	organonitrogen compound metabolic process	3	1
GO:1901565	organonitrogen compound catabolic process	4	1

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	29
GO:0003824	catalytic activity	1	29
GO:0004518	nuclease activity	4	29
GO:0004519	endonuclease activity	5	29
GO:0005488	binding	1	29
GO:0016787	hydrolase activity	2	29
GO:0016788	hydrolase activity, acting on ester bonds	3	29
GO:0043167	ion binding	2	29
GO:0043169	cation binding	3	29
GO:0046872	metal ion binding	4	29
GO:0097159	organic cyclic compound binding	2	29
GO:1901363	heterocyclic compound binding	2	29
GO:0008252	nucleotidase activity	6	1
GO:0008254	3'-nucleotidase activity	7	1
GO:0016791	phosphatase activity	5	1
GO:0042578	phosphoric ester hydrolase activity	4	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	282	284	PF00675	0.475
CLV_PCSK_KEX2_1	282	284	PF00082	0.470
CLV_PCSK_SKI1_1	187	191	PF00082	0.543
CLV_PCSK_SKI1_1	256	260	PF00082	0.374
CLV_PCSK_SKI1_1	277	281	PF00082	0.432
CLV_PCSK_SKI1_1	282	286	PF00082	0.423
CLV_PCSK_SKI1_1	290	294	PF00082	0.443
CLV_PCSK_SKI1_1	48	52	PF00082	0.409
DOC_CKS1_1	96	101	PF01111	0.264
DOC_MAPK_MEF2A_6	32	39	PF00069	0.372
DOC_MAPK_MEF2A_6	6	14	PF00069	0.454
DOC_PP2B_LxvP_1	233	236	PF13499	0.163
DOC_USP7_MATH_1	23	27	PF00917	0.342
DOC_USP7_UBL2_3	273	277	PF12436	0.292
DOC_USP7_UBL2_3	48	52	PF12436	0.203
DOC_WW_Pin1_4	108	113	PF00397	0.281
DOC_WW_Pin1_4	66	71	PF00397	0.315
DOC_WW_Pin1_4	95	100	PF00397	0.266
LIG_14-3-3_CanoR_1	333	341	PF00244	0.285
LIG_14-3-3_CanoR_1	366	374	PF00244	0.601
LIG_Actin_WH2_2	287	302	PF00022	0.235
LIG_BIR_II_1	1	5	PF00653	0.622
LIG_BIR_III_3	1	5	PF00653	0.551
LIG_eIF4E_1	11	17	PF01652	0.170
LIG_FHA_1	113	119	PF00498	0.314
LIG_FHA_1	133	139	PF00498	0.320
LIG_FHA_1	184	190	PF00498	0.281
LIG_FHA_1	241	247	PF00498	0.281
LIG_FHA_1	322	328	PF00498	0.530
LIG_FHA_1	338	344	PF00498	0.350
LIG_FHA_1	354	360	PF00498	0.327
LIG_FHA_1	5	11	PF00498	0.404
LIG_FHA_2	232	238	PF00498	0.359
LIG_FHA_2	327	333	PF00498	0.422
LIG_GBD_Chelix_1	291	299	PF00786	0.488
LIG_LIR_Apic_2	203	209	PF02991	0.275
LIG_LIR_Gen_1	213	222	PF02991	0.277
LIG_LIR_Nem_3	107	113	PF02991	0.338
LIG_LIR_Nem_3	213	218	PF02991	0.277
LIG_PDZ_Class_2	377	382	PF00595	0.578
LIG_Pex14_2	106	110	PF04695	0.153
LIG_Pex14_2	145	149	PF04695	0.163
LIG_Pex14_2	259	263	PF04695	0.160
LIG_Pex14_2	91	95	PF04695	0.174
LIG_SH2_CRK	206	210	PF00017	0.288
LIG_SH2_CRK	257	261	PF00017	0.257
LIG_SH2_PTP2	11	14	PF00017	0.170
LIG_SH2_SRC	224	227	PF00017	0.359
LIG_SH2_STAT5	11	14	PF00017	0.421
LIG_SH2_STAT5	224	227	PF00017	0.273
LIG_SH3_3	19	25	PF00018	0.186
LIG_SH3_3	371	377	PF00018	0.534
LIG_SH3_3	64	70	PF00018	0.253
LIG_SH3_3	96	102	PF00018	0.259
LIG_SH3_5	168	172	PF00018	0.174
LIG_SUMO_SIM_par_1	177	183	PF11976	0.253
LIG_SUMO_SIM_par_1	376	381	PF11976	0.530
LIG_TRAF2_1	329	332	PF00917	0.351
LIG_WRC_WIRS_1	327	332	PF05994	0.345
MOD_CK1_1	104	110	PF00069	0.296
MOD_CK1_1	127	133	PF00069	0.324
MOD_CK1_1	183	189	PF00069	0.392
MOD_CK1_1	303	309	PF00069	0.352
MOD_CK2_1	193	199	PF00069	0.333
MOD_CK2_1	231	237	PF00069	0.310
MOD_CK2_1	326	332	PF00069	0.479
MOD_GlcNHglycan	126	129	PF01048	0.546
MOD_GlcNHglycan	84	87	PF01048	0.433
MOD_GSK3_1	104	111	PF00069	0.258
MOD_GSK3_1	129	136	PF00069	0.325
MOD_GSK3_1	200	207	PF00069	0.206
MOD_GSK3_1	240	247	PF00069	0.302
MOD_GSK3_1	267	274	PF00069	0.163
MOD_GSK3_1	294	301	PF00069	0.282
MOD_GSK3_1	322	329	PF00069	0.461
MOD_GSK3_1	333	340	PF00069	0.446
MOD_GSK3_1	82	89	PF00069	0.382
MOD_N-GLC_1	183	188	PF02516	0.467
MOD_N-GLC_1	231	236	PF02516	0.474
MOD_N-GLC_1	241	246	PF02516	0.457
MOD_N-GLC_1	295	300	PF02516	0.560
MOD_N-GLC_1	322	327	PF02516	0.568
MOD_NEK2_1	124	129	PF00069	0.330
MOD_NEK2_1	225	230	PF00069	0.288
MOD_NEK2_1	240	245	PF00069	0.290
MOD_NEK2_1	294	299	PF00069	0.310
MOD_NEK2_1	321	326	PF00069	0.425
MOD_NEK2_1	353	358	PF00069	0.207
MOD_NEK2_1	360	365	PF00069	0.360
MOD_NEK2_1	82	87	PF00069	0.280
MOD_PIKK_1	137	143	PF00454	0.174
MOD_Plk_1	104	110	PF00069	0.257
MOD_Plk_1	225	231	PF00069	0.267
MOD_Plk_1	241	247	PF00069	0.291
MOD_Plk_1	295	301	PF00069	0.332
MOD_Plk_1	321	327	PF00069	0.518
MOD_Plk_4	117	123	PF00069	0.255
MOD_Plk_4	303	309	PF00069	0.338
MOD_Plk_4	322	328	PF00069	0.329
MOD_ProDKin_1	108	114	PF00069	0.281
MOD_ProDKin_1	66	72	PF00069	0.315
MOD_ProDKin_1	95	101	PF00069	0.266
MOD_SUMO_rev_2	269	274	PF00179	0.326
TRG_DiLeu_BaEn_1	281	286	PF01217	0.235
TRG_ENDOCYTIC_2	11	14	PF00928	0.386
TRG_ENDOCYTIC_2	215	218	PF00928	0.274
TRG_ER_diArg_1	282	284	PF00400	0.275
TRG_Pf-PMV_PEXEL_1	208	213	PF00026	0.523

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I8Q7	Leptomonas seymouri	37%	100%
A0A0S4JAL3	Bodo saltans	42%	100%
A0A0S4JML7	Bodo saltans	26%	100%
A0A1X0P2K7	Trypanosomatidae	44%	100%
A0A3Q8IBF6	Leishmania donovani	48%	100%
A0A3Q8IEW3	Leishmania donovani	38%	100%
A0A3Q8IFH4	Leishmania donovani	65%	100%
A0A3Q8IK90	Leishmania donovani	90%	100%
A0A3S7X3A6	Leishmania donovani	38%	100%
A0A422N7B0	Trypanosoma rangeli	40%	100%
A4H6V5	Leishmania braziliensis	47%	100%
A4HI96	Leishmania braziliensis	33%	100%
A4HJL3	Leishmania braziliensis	62%	100%
A4I5I0	Leishmania infantum	38%	100%
A4I736	Leishmania infantum	65%	100%
C9ZQH5	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	40%	100%
C9ZQH6	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	40%	100%
C9ZQW0	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	37%	100%
E9AGC7	Leishmania infantum	48%	100%
E9ANW7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	49%	100%
E9B0S4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	36%	100%
E9B0S5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	35%	100%
E9B231	Leishmania mexicana (strain MHOM/GT/2001/U1103)	64%	100%
F4JJL0	Arabidopsis thaliana	27%	100%
F4JJL3	Arabidopsis thaliana	25%	100%
Q4Q630	Leishmania major	61%	100%
Q4Q7F3	Leishmania major	37%	100%
Q4Q7F4	Leishmania major	37%	100%
Q4QGQ3	Leishmania major	49%	100%
Q8LDW6	Arabidopsis thaliana	27%	100%
Q9C9G4	Arabidopsis thaliana	29%	100%
Q9SXA6	Arabidopsis thaliana	29%	100%
V5B8Z9	Trypanosoma cruzi	39%	98%