Putative ubiquitin-conjugating enzyme

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

ubiquitin-conjugating enzyme E2, putative

Species:

Leishmania major

UniProt:

Q4QJA9_LEIMA

TriTrypDb:

LmjF.05.0930 , LMJLV39_050014200 , LMJSD75_050014500

Length:

464

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 6
NetGPI	no	yes: 0, no: 6

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	2
GO:0016020	membrane	2	4
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	4

Expansion

Sequence features

Q4QJA9

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QJA9

Function

Biological processes
Term	Name	Level	Count
GO:0000209	protein polyubiquitination	8	2
GO:0006807	nitrogen compound metabolic process	2	2
GO:0008152	metabolic process	1	2
GO:0016567	protein ubiquitination	7	2
GO:0019538	protein metabolic process	3	2
GO:0032446	protein modification by small protein conjugation	6	2
GO:0036211	protein modification process	4	2
GO:0043170	macromolecule metabolic process	3	2
GO:0043412	macromolecule modification	4	2
GO:0044238	primary metabolic process	2	2
GO:0070647	protein modification by small protein conjugation or removal	5	2
GO:0071704	organic substance metabolic process	2	2
GO:1901564	organonitrogen compound metabolic process	3	2

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	4
GO:0004842	ubiquitin-protein transferase activity	4	2
GO:0016740	transferase activity	2	2
GO:0016874	ligase activity	2	4
GO:0019787	ubiquitin-like protein transferase activity	3	2
GO:0061631	ubiquitin conjugating enzyme activity	5	2
GO:0061650	ubiquitin-like protein conjugating enzyme activity	4	2
GO:0140096	catalytic activity, acting on a protein	2	2

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	238	242	PF00656	0.457
CLV_C14_Caspase3-7	271	275	PF00656	0.484
CLV_NRD_NRD_1	16	18	PF00675	0.469
CLV_NRD_NRD_1	455	457	PF00675	0.304
CLV_PCSK_KEX2_1	16	18	PF00082	0.469
CLV_PCSK_KEX2_1	455	457	PF00082	0.304
CLV_PCSK_KEX2_1	9	11	PF00082	0.469
CLV_PCSK_PC1ET2_1	9	11	PF00082	0.469
CLV_PCSK_SKI1_1	421	425	PF00082	0.660
DEG_APCC_DBOX_1	454	462	PF00400	0.440
DEG_SCF_FBW7_1	193	200	PF00400	0.451
DEG_SCF_FBW7_1	225	232	PF00400	0.475
DEG_SCF_FBW7_2	360	367	PF00400	0.466
DOC_CKS1_1	194	199	PF01111	0.452
DOC_CKS1_1	289	294	PF01111	0.515
DOC_CYCLIN_yCln2_LP_2	233	236	PF00134	0.453
DOC_MAPK_RevD_3	42	58	PF00069	0.222
DOC_MAPK_RevD_3	442	456	PF00069	0.366
DOC_PP1_RVXF_1	419	425	PF00149	0.430
DOC_PP2B_LxvP_1	187	190	PF13499	0.458
DOC_PP2B_LxvP_1	233	236	PF13499	0.446
DOC_PP2B_LxvP_1	73	76	PF13499	0.269
DOC_SPAK_OSR1_1	86	90	PF12202	0.316
DOC_USP7_MATH_1	197	201	PF00917	0.503
DOC_USP7_MATH_1	227	231	PF00917	0.511
DOC_USP7_MATH_1	240	244	PF00917	0.615
DOC_USP7_MATH_1	267	271	PF00917	0.515
DOC_USP7_MATH_1	384	388	PF00917	0.463
DOC_USP7_MATH_1	399	403	PF00917	0.464
DOC_WW_Pin1_4	174	179	PF00397	0.439
DOC_WW_Pin1_4	193	198	PF00397	0.420
DOC_WW_Pin1_4	217	222	PF00397	0.641
DOC_WW_Pin1_4	225	230	PF00397	0.489
DOC_WW_Pin1_4	259	264	PF00397	0.524
DOC_WW_Pin1_4	277	282	PF00397	0.531
DOC_WW_Pin1_4	285	290	PF00397	0.495
DOC_WW_Pin1_4	324	329	PF00397	0.532
DOC_WW_Pin1_4	360	365	PF00397	0.467
DOC_WW_Pin1_4	379	384	PF00397	0.447
DOC_WW_Pin1_4	401	406	PF00397	0.430
LIG_14-3-3_CanoR_1	456	462	PF00244	0.237
LIG_Actin_WH2_2	442	457	PF00022	0.396
LIG_APCC_ABBA_1	170	175	PF00400	0.376
LIG_BIR_II_1	1	5	PF00653	0.372
LIG_BIR_III_2	380	384	PF00653	0.460
LIG_BRCT_BRCA1_1	444	448	PF00533	0.279
LIG_BRCT_BRCA1_1	83	87	PF00533	0.316
LIG_CtBP_PxDLS_1	356	360	PF00389	0.418
LIG_deltaCOP1_diTrp_1	144	153	PF00928	0.349
LIG_deltaCOP1_diTrp_1	39	42	PF00928	0.269
LIG_EH1_1	447	455	PF00400	0.272
LIG_EVH1_1	187	191	PF00568	0.492
LIG_FHA_1	106	112	PF00498	0.269
LIG_FHA_1	160	166	PF00498	0.377
LIG_FHA_1	280	286	PF00498	0.546
LIG_FHA_1	380	386	PF00498	0.494
LIG_FHA_2	236	242	PF00498	0.451
LIG_FHA_2	269	275	PF00498	0.554
LIG_GBD_Chelix_1	443	451	PF00786	0.323
LIG_LIR_Apic_2	27	32	PF02991	0.316
LIG_LIR_Gen_1	445	454	PF02991	0.297
LIG_LIR_Nem_3	12	18	PF02991	0.269
LIG_LIR_Nem_3	445	451	PF02991	0.305
LIG_MYND_1	185	189	PF01753	0.441
LIG_NRBOX	457	463	PF00104	0.351
LIG_Pex14_1	105	109	PF04695	0.269
LIG_Pex14_2	38	42	PF04695	0.269
LIG_PROFILIN_1	188	194	PF00235	0.455
LIG_REV1ctd_RIR_1	78	87	PF16727	0.269
LIG_SH2_CRK	15	19	PF00017	0.268
LIG_SH2_PTP2	29	32	PF00017	0.284
LIG_SH2_SRC	109	112	PF00017	0.350
LIG_SH2_STAP1	109	113	PF00017	0.269
LIG_SH2_STAP1	3	7	PF00017	0.322
LIG_SH2_STAT5	29	32	PF00017	0.284
LIG_SH3_1	186	192	PF00018	0.461
LIG_SH3_1	260	266	PF00018	0.493
LIG_SH3_1	69	75	PF00018	0.294
LIG_SH3_3	182	188	PF00018	0.480
LIG_SH3_3	191	197	PF00018	0.469
LIG_SH3_3	218	224	PF00018	0.504
LIG_SH3_3	247	253	PF00018	0.481
LIG_SH3_3	260	266	PF00018	0.465
LIG_SH3_3	286	292	PF00018	0.481
LIG_SH3_3	329	335	PF00018	0.471
LIG_SH3_3	337	343	PF00018	0.476
LIG_SH3_3	402	408	PF00018	0.426
LIG_SH3_3	409	415	PF00018	0.460
LIG_SH3_3	428	434	PF00018	0.433
LIG_SH3_3	69	75	PF00018	0.294
LIG_TRFH_1	71	75	PF08558	0.304
LIG_WRC_WIRS_1	443	448	PF05994	0.325
MOD_CDC14_SPxK_1	177	180	PF00782	0.553
MOD_CDC14_SPxK_1	327	330	PF00782	0.638
MOD_CDK_SPxK_1	174	180	PF00069	0.539
MOD_CDK_SPxK_1	324	330	PF00069	0.636
MOD_CK1_1	2	8	PF00069	0.397
MOD_CK1_1	404	410	PF00069	0.546
MOD_CK1_1	95	101	PF00069	0.304
MOD_CK2_1	424	430	PF00069	0.451
MOD_GlcNHglycan	199	202	PF01048	0.644
MOD_GlcNHglycan	225	228	PF01048	0.624
MOD_GlcNHglycan	302	305	PF01048	0.693
MOD_GlcNHglycan	386	389	PF01048	0.610
MOD_GlcNHglycan	401	404	PF01048	0.447
MOD_GlcNHglycan	5	8	PF01048	0.339
MOD_GlcNHglycan	83	86	PF01048	0.496
MOD_GSK3_1	145	152	PF00069	0.393
MOD_GSK3_1	193	200	PF00069	0.609
MOD_GSK3_1	223	230	PF00069	0.670
MOD_GSK3_1	379	386	PF00069	0.560
MOD_GSK3_1	457	464	PF00069	0.237
MOD_GSK3_1	92	99	PF00069	0.324
MOD_NEK2_1	113	118	PF00069	0.304
MOD_NEK2_1	153	158	PF00069	0.553
MOD_NEK2_1	268	273	PF00069	0.608
MOD_NEK2_1	424	429	PF00069	0.551
MOD_NEK2_1	65	70	PF00069	0.330
MOD_NEK2_1	79	84	PF00069	0.307
MOD_NEK2_2	40	45	PF00069	0.329
MOD_PIKK_1	165	171	PF00454	0.418
MOD_PIKK_1	208	214	PF00454	0.579
MOD_PKA_1	9	15	PF00069	0.304
MOD_PKA_2	139	145	PF00069	0.341
MOD_PKA_2	9	15	PF00069	0.304
MOD_Plk_1	429	435	PF00069	0.545
MOD_Plk_1	50	56	PF00069	0.316
MOD_Plk_4	229	235	PF00069	0.617
MOD_Plk_4	268	274	PF00069	0.554
MOD_Plk_4	355	361	PF00069	0.620
MOD_Plk_4	424	430	PF00069	0.571
MOD_Plk_4	442	448	PF00069	0.369
MOD_Plk_4	457	463	PF00069	0.210
MOD_ProDKin_1	174	180	PF00069	0.543
MOD_ProDKin_1	193	199	PF00069	0.516
MOD_ProDKin_1	217	223	PF00069	0.822
MOD_ProDKin_1	225	231	PF00069	0.614
MOD_ProDKin_1	259	265	PF00069	0.658
MOD_ProDKin_1	277	283	PF00069	0.668
MOD_ProDKin_1	285	291	PF00069	0.619
MOD_ProDKin_1	324	330	PF00069	0.674
MOD_ProDKin_1	360	366	PF00069	0.578
MOD_ProDKin_1	379	385	PF00069	0.555
MOD_ProDKin_1	401	407	PF00069	0.528
MOD_SUMO_for_1	203	206	PF00179	0.630
TRG_DiLeu_BaLyEn_6	433	438	PF01217	0.453
TRG_ENDOCYTIC_2	15	18	PF00928	0.326
TRG_ER_diArg_1	15	17	PF00400	0.303
TRG_ER_diArg_1	454	456	PF00400	0.304

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I1C5	Leptomonas seymouri	43%	95%
A0A3S5H5K9	Leishmania donovani	81%	100%
A4H4D6	Leishmania braziliensis	60%	100%
A4HSL5	Leishmania infantum	81%	100%
E9AKJ9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	69%	100%