NAD(P)-dependent steroid dehydrogenase-like protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

NAD(P)-dependent steroid dehydrogenase protein, putative

Species:

Leishmania major

UniProt:

Q4QJ44_LEIMA

TriTrypDb:

LmjF.06.0350 , LMJLV39_060008900 , LMJSD75_060009000

Length:

378

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 9

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	10
GO:0110165	cellular anatomical entity	1	10

Expansion

Sequence features

Q4QJ44

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QJ44

Function

Biological processes
Term	Name	Level	Count
GO:0006629	lipid metabolic process	3	12
GO:0006694	steroid biosynthetic process	5	12
GO:0008152	metabolic process	1	12
GO:0008202	steroid metabolic process	4	12
GO:0008610	lipid biosynthetic process	4	12
GO:0009058	biosynthetic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0071704	organic substance metabolic process	2	12
GO:1901360	organic cyclic compound metabolic process	3	12
GO:1901362	organic cyclic compound biosynthetic process	4	12
GO:1901576	organic substance biosynthetic process	3	12

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	12
GO:0003854	3-beta-hydroxy-delta5-steroid dehydrogenase activity	5	12
GO:0016229	steroid dehydrogenase activity	3	12
GO:0016491	oxidoreductase activity	2	12
GO:0016614	oxidoreductase activity, acting on CH-OH group of donors	3	12
GO:0016616	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor	4	12
GO:0033764	steroid dehydrogenase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor	4	12
GO:0000252	C-3 sterol dehydrogenase (C-4 sterol decarboxylase) activity	5	1
GO:0047012	sterol-4-alpha-carboxylate 3-dehydrogenase (decarboxylating) activity	5	1
GO:0103066	4alpha-carboxy-4beta-methyl-5alpha-cholesta-8-en-3beta-ol:NAD(P)+ 3-oxidoreductase (decarboxylating) activity	5	1
GO:0103067	4alpha-carboxy-5alpha-cholesta-8-en-3beta-ol:NAD(P)+ 3-dehydrogenase (decarboxylating) activity	5	1
GO:0102294	cholesterol dehydrogenase activity	5	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	210	214	PF00656	0.455
CLV_NRD_NRD_1	374	376	PF00675	0.702
CLV_NRD_NRD_1	85	87	PF00675	0.305
CLV_PCSK_KEX2_1	321	323	PF00082	0.460
CLV_PCSK_KEX2_1	85	87	PF00082	0.341
CLV_PCSK_PC1ET2_1	321	323	PF00082	0.460
CLV_PCSK_SKI1_1	106	110	PF00082	0.316
DEG_MDM2_SWIB_1	332	340	PF02201	0.283
DEG_Nend_Nbox_1	1	3	PF02207	0.352
DOC_ANK_TNKS_1	123	130	PF00023	0.516
DOC_PP1_RVXF_1	204	210	PF00149	0.455
DOC_PP1_RVXF_1	22	29	PF00149	0.455
DOC_PP1_RVXF_1	320	327	PF00149	0.257
DOC_PP1_RVXF_1	342	348	PF00149	0.337
DOC_PP2B_LxvP_1	183	186	PF13499	0.455
DOC_PP4_FxxP_1	122	125	PF00568	0.450
DOC_SPAK_OSR1_1	235	239	PF12202	0.541
DOC_USP7_UBL2_3	224	228	PF12436	0.541
DOC_USP7_UBL2_3	256	260	PF12436	0.493
DOC_WW_Pin1_4	109	114	PF00397	0.449
DOC_WW_Pin1_4	227	232	PF00397	0.455
LIG_14-3-3_CanoR_1	206	215	PF00244	0.455
LIG_APCC_ABBA_1	329	334	PF00400	0.324
LIG_BRCT_BRCA1_1	113	117	PF00533	0.467
LIG_deltaCOP1_diTrp_1	334	343	PF00928	0.283
LIG_EH1_1	101	109	PF00400	0.516
LIG_FHA_1	192	198	PF00498	0.455
LIG_FHA_1	296	302	PF00498	0.388
LIG_FHA_1	4	10	PF00498	0.424
LIG_FHA_1	90	96	PF00498	0.449
LIG_FHA_2	129	135	PF00498	0.455
LIG_FHA_2	148	154	PF00498	0.516
LIG_FHA_2	208	214	PF00498	0.455
LIG_FHA_2	238	244	PF00498	0.472
LIG_LIR_Apic_2	120	125	PF02991	0.473
LIG_LIR_Gen_1	141	148	PF02991	0.457
LIG_LIR_Gen_1	53	62	PF02991	0.481
LIG_LIR_Nem_3	163	169	PF02991	0.515
LIG_LIR_Nem_3	323	329	PF02991	0.293
LIG_LIR_Nem_3	339	345	PF02991	0.286
LIG_LIR_Nem_3	53	58	PF02991	0.481
LIG_LIR_Nem_3	82	87	PF02991	0.464
LIG_MAD2	24	32	PF02301	0.455
LIG_Pex14_1	347	351	PF04695	0.298
LIG_Pex14_2	332	336	PF04695	0.282
LIG_Pex14_2	343	347	PF04695	0.296
LIG_PTB_Apo_2	226	233	PF02174	0.505
LIG_PTB_Phospho_1	226	232	PF10480	0.505
LIG_SH2_CRK	84	88	PF00017	0.516
LIG_SH2_NCK_1	55	59	PF00017	0.480
LIG_SH2_STAT5	215	218	PF00017	0.541
LIG_SH2_STAT5	232	235	PF00017	0.375
LIG_SH2_STAT5	237	240	PF00017	0.440
LIG_SH2_STAT5	251	254	PF00017	0.348
LIG_SH2_STAT5	282	285	PF00017	0.324
LIG_SH2_STAT5	47	50	PF00017	0.466
LIG_SH3_3	133	139	PF00018	0.448
LIG_SH3_3	173	179	PF00018	0.455
LIG_SH3_3	271	277	PF00018	0.590
LIG_SH3_3	325	331	PF00018	0.328
LIG_SH3_3	74	80	PF00018	0.452
LIG_TYR_ITSM	51	58	PF00017	0.516
MOD_CDK_SPxxK_3	109	116	PF00069	0.455
MOD_CK1_1	111	117	PF00069	0.455
MOD_CK1_1	363	369	PF00069	0.429
MOD_CK1_1	56	62	PF00069	0.560
MOD_CK2_1	128	134	PF00069	0.455
MOD_CK2_1	147	153	PF00069	0.516
MOD_CK2_1	241	247	PF00069	0.490
MOD_GlcNHglycan	363	366	PF01048	0.664
MOD_GlcNHglycan	57	61	PF01048	0.360
MOD_GSK3_1	107	114	PF00069	0.447
MOD_GSK3_1	237	244	PF00069	0.446
MOD_N-GLC_1	117	122	PF02516	0.372
MOD_N-GLC_1	336	341	PF02516	0.598
MOD_N-GLC_2	214	216	PF02516	0.341
MOD_NEK2_1	107	112	PF00069	0.446
MOD_NEK2_1	117	122	PF00069	0.324
MOD_NEK2_1	295	300	PF00069	0.375
MOD_NEK2_1	307	312	PF00069	0.243
MOD_NEK2_1	8	13	PF00069	0.476
MOD_PIKK_1	363	369	PF00454	0.380
MOD_PKA_2	158	164	PF00069	0.533
MOD_Plk_1	336	342	PF00069	0.357
MOD_Plk_1	354	360	PF00069	0.290
MOD_Plk_1	50	56	PF00069	0.521
MOD_Plk_2-3	128	134	PF00069	0.455
MOD_Plk_2-3	51	57	PF00069	0.533
MOD_Plk_4	117	123	PF00069	0.467
MOD_Plk_4	266	272	PF00069	0.542
MOD_Plk_4	355	361	PF00069	0.429
MOD_ProDKin_1	109	115	PF00069	0.449
MOD_ProDKin_1	227	233	PF00069	0.455
TRG_ENDOCYTIC_2	143	146	PF00928	0.464
TRG_ENDOCYTIC_2	251	254	PF00928	0.469
TRG_ENDOCYTIC_2	55	58	PF00928	0.467
TRG_ENDOCYTIC_2	84	87	PF00928	0.452
TRG_ER_diArg_1	84	86	PF00400	0.533
TRG_Pf-PMV_PEXEL_1	322	327	PF00026	0.461

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P6H3	Leptomonas seymouri	89%	93%
A0A0S4J083	Bodo saltans	67%	92%
A0A1X0NKS1	Trypanosomatidae	73%	93%
A0A3R7NBA3	Trypanosoma rangeli	72%	93%
A0A3S5H5P6	Leishmania donovani	98%	93%
A4H4K0	Leishmania braziliensis	92%	100%
A4HSS6	Leishmania infantum	98%	93%
A6NKP2	Homo sapiens	26%	90%
A8DZE7	Danio rerio	27%	98%
A9X4U2	Arabidopsis thaliana	27%	67%
B2FI29	Stenotrophomonas maltophilia (strain K279a)	28%	100%
C9ZTC5	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	71%	94%
E9AKR3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	95%	100%
O35296	Mesocricetus auratus	22%	100%
O35469	Mus musculus	24%	100%
O43050	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	26%	100%
O46516	Equus caballus	22%	100%
O57245	Vaccinia virus (strain Ankara)	21%	100%
P14893	Bos taurus	21%	100%
P21097	Vaccinia virus (strain Copenhagen)	21%	100%
P22071	Rattus norvegicus	25%	100%
P24815	Mus musculus	24%	100%
P26149	Mus musculus	25%	100%
P26150	Mus musculus	25%	100%
P26670	Vaccinia virus (strain Western Reserve)	21%	100%
P27364	Rattus norvegicus	23%	100%
P53199	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	27%	100%
P75821	Escherichia coli (strain K12)	23%	100%
P9WN64	Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)	25%	100%
P9WN65	Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)	25%	100%
P9WQP6	Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)	27%	100%
P9WQP7	Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)	27%	100%
Q0IH73	Xenopus laevis	27%	98%
Q15738	Homo sapiens	32%	100%
Q32L94	Bos taurus	25%	96%
Q3ZBE9	Bos taurus	31%	100%
Q4R7R1	Macaca fascicularis	25%	96%
Q5A1B0	Candida albicans (strain SC5314 / ATCC MYA-2876)	27%	100%
Q5PPL3	Rattus norvegicus	28%	100%
Q60555	Mesocricetus auratus	23%	100%
Q62878	Rattus norvegicus	24%	100%
Q64421	Mesocricetus auratus	23%	100%
Q67ZE1	Arabidopsis thaliana	31%	67%
Q8EG63	Shewanella oneidensis (strain MR-1)	25%	98%
Q8PDW5	Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25)	31%	100%
Q8WUS8	Homo sapiens	25%	96%
Q9D665	Mus musculus	27%	96%
Q9FX01	Arabidopsis thaliana	31%	86%
Q9H2F3	Homo sapiens	25%	100%
Q9N119	Sus scrofa	22%	100%
Q9R1J0	Mus musculus	28%	100%
Q9RR28	Streptomyces antibioticus	24%	100%
Q9XWF0	Caenorhabditis elegans	24%	82%
V5BEU6	Trypanosoma cruzi	71%	93%