Putative glutamine synthetase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

glutamine synthetase, putative

Species:

Leishmania major

UniProt:

Q4QJ42_LEIMA

TriTrypDb:

LmjF.06.0370 , LMJLV39_060009200 , LMJSD75_060009200

Length:

536

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 1
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	yes	yes: 4
Pissara et al.	yes	yes: 12

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 7

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 12
NetGPI	no	yes: 0, no: 12

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

Q4QJ42

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QJ42

Function

Biological processes
Term	Name	Level	Count
GO:0006082	organic acid metabolic process	3	13
GO:0006520	amino acid metabolic process	3	13
GO:0006541	glutamine metabolic process	6	13
GO:0006542	glutamine biosynthetic process	7	13
GO:0006807	nitrogen compound metabolic process	2	13
GO:0008152	metabolic process	1	13
GO:0008652	amino acid biosynthetic process	4	13
GO:0009058	biosynthetic process	2	13
GO:0009064	glutamine family amino acid metabolic process	5	13
GO:0009084	glutamine family amino acid biosynthetic process	6	13
GO:0009987	cellular process	1	13
GO:0016053	organic acid biosynthetic process	4	13
GO:0019752	carboxylic acid metabolic process	5	13
GO:0043436	oxoacid metabolic process	4	13
GO:0044237	cellular metabolic process	2	13
GO:0044238	primary metabolic process	2	13
GO:0044249	cellular biosynthetic process	3	13
GO:0044281	small molecule metabolic process	2	13
GO:0044283	small molecule biosynthetic process	3	13
GO:0046394	carboxylic acid biosynthetic process	5	13
GO:0071704	organic substance metabolic process	2	13
GO:1901564	organonitrogen compound metabolic process	3	13
GO:1901566	organonitrogen compound biosynthetic process	4	13
GO:1901576	organic substance biosynthetic process	3	13
GO:1901605	alpha-amino acid metabolic process	4	13
GO:1901607	alpha-amino acid biosynthetic process	5	13

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	13
GO:0004356	glutamate-ammonia ligase activity	6	13
GO:0016211	ammonia ligase activity	5	13
GO:0016874	ligase activity	2	13
GO:0016879	ligase activity, forming carbon-nitrogen bonds	3	13
GO:0016880	acid-ammonia (or amide) ligase activity	4	13

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	174	178	PF00656	0.531
CLV_C14_Caspase3-7	388	392	PF00656	0.565
CLV_NRD_NRD_1	328	330	PF00675	0.298
CLV_NRD_NRD_1	38	40	PF00675	0.681
CLV_NRD_NRD_1	492	494	PF00675	0.314
CLV_PCSK_KEX2_1	492	494	PF00082	0.298
CLV_PCSK_SKI1_1	201	205	PF00082	0.307
CLV_PCSK_SKI1_1	378	382	PF00082	0.298
CLV_PCSK_SKI1_1	532	536	PF00082	0.629
DEG_APCC_DBOX_1	377	385	PF00400	0.507
DEG_SPOP_SBC_1	242	246	PF00917	0.584
DOC_CDC14_PxL_1	99	107	PF14671	0.428
DOC_CKS1_1	514	519	PF01111	0.532
DOC_CYCLIN_yCln2_LP_2	230	236	PF00134	0.531
DOC_MAPK_DCC_7	243	253	PF00069	0.507
DOC_MAPK_gen_1	183	191	PF00069	0.516
DOC_MAPK_MEF2A_6	70	78	PF00069	0.492
DOC_PP1_RVXF_1	437	444	PF00149	0.531
DOC_PP2B_PxIxI_1	248	254	PF00149	0.507
DOC_PP4_FxxP_1	310	313	PF00568	0.512
DOC_USP7_MATH_1	138	142	PF00917	0.508
DOC_USP7_MATH_1	152	156	PF00917	0.668
DOC_USP7_MATH_1	242	246	PF00917	0.547
DOC_USP7_MATH_1	480	484	PF00917	0.592
DOC_USP7_UBL2_3	435	439	PF12436	0.565
DOC_WW_Pin1_4	139	144	PF00397	0.745
DOC_WW_Pin1_4	351	356	PF00397	0.589
DOC_WW_Pin1_4	513	518	PF00397	0.595
DOC_WW_Pin1_4	520	525	PF00397	0.490
LIG_14-3-3_CanoR_1	167	171	PF00244	0.498
LIG_14-3-3_CanoR_1	243	251	PF00244	0.476
LIG_14-3-3_CanoR_1	270	277	PF00244	0.531
LIG_14-3-3_CanoR_1	432	438	PF00244	0.523
LIG_14-3-3_CanoR_1	471	478	PF00244	0.537
LIG_14-3-3_CanoR_1	506	514	PF00244	0.498
LIG_14-3-3_CanoR_1	89	93	PF00244	0.706
LIG_BIR_III_2	497	501	PF00653	0.518
LIG_BRCT_BRCA1_1	504	508	PF00533	0.518
LIG_deltaCOP1_diTrp_1	527	535	PF00928	0.479
LIG_deltaCOP1_diTrp_1	69	73	PF00928	0.425
LIG_FHA_1	151	157	PF00498	0.760
LIG_FHA_1	163	169	PF00498	0.533
LIG_FHA_1	186	192	PF00498	0.490
LIG_FHA_1	225	231	PF00498	0.511
LIG_FHA_1	456	462	PF00498	0.537
LIG_FHA_1	502	508	PF00498	0.589
LIG_FHA_1	514	520	PF00498	0.492
LIG_FHA_1	94	100	PF00498	0.692
LIG_FHA_2	270	276	PF00498	0.524
LIG_FHA_2	347	353	PF00498	0.505
LIG_FHA_2	415	421	PF00498	0.518
LIG_IRF3_LxIS_1	340	347	PF10401	0.565
LIG_LIR_Gen_1	108	116	PF02991	0.643
LIG_LIR_Gen_1	169	177	PF02991	0.498
LIG_LIR_Gen_1	69	79	PF02991	0.434
LIG_LIR_Nem_3	108	113	PF02991	0.644
LIG_LIR_Nem_3	169	173	PF02991	0.498
LIG_LIR_Nem_3	283	289	PF02991	0.436
LIG_LIR_Nem_3	69	74	PF02991	0.427
LIG_LYPXL_yS_3	127	130	PF13949	0.495
LIG_NRBOX	380	386	PF00104	0.589
LIG_PCNA_yPIPBox_3	373	383	PF02747	0.507
LIG_Pex14_1	327	331	PF04695	0.604
LIG_PTB_Apo_2	203	210	PF02174	0.604
LIG_REV1ctd_RIR_1	113	122	PF16727	0.491
LIG_SH2_CRK	121	125	PF00017	0.493
LIG_SH2_PTP2	170	173	PF00017	0.498
LIG_SH2_SRC	258	261	PF00017	0.589
LIG_SH2_STAP1	336	340	PF00017	0.498
LIG_SH2_STAT3	190	193	PF00017	0.589
LIG_SH2_STAT5	170	173	PF00017	0.498
LIG_SH2_STAT5	190	193	PF00017	0.368
LIG_SH2_STAT5	258	261	PF00017	0.580
LIG_SH2_STAT5	293	296	PF00017	0.547
LIG_SH2_STAT5	318	321	PF00017	0.494
LIG_SH2_STAT5	429	432	PF00017	0.498
LIG_SH2_STAT5	501	504	PF00017	0.498
LIG_SH3_3	122	128	PF00018	0.492
LIG_SUMO_SIM_par_1	250	256	PF11976	0.502
LIG_SxIP_EBH_1	243	254	PF03271	0.589
LIG_TYR_ITIM	124	129	PF00017	0.489
LIG_TYR_ITIM	168	173	PF00017	0.507
LIG_UBA3_1	129	137	PF00899	0.498
LIG_UBA3_1	46	54	PF00899	0.389
MOD_CK1_1	141	147	PF00069	0.513
MOD_CK1_1	269	275	PF00069	0.549
MOD_CK1_1	320	326	PF00069	0.587
MOD_CK1_1	413	419	PF00069	0.543
MOD_CK1_1	459	465	PF00069	0.589
MOD_CK1_1	88	94	PF00069	0.732
MOD_CK2_1	269	275	PF00069	0.527
MOD_CK2_1	346	352	PF00069	0.507
MOD_CK2_1	414	420	PF00069	0.506
MOD_Cter_Amidation	327	330	PF01082	0.298
MOD_GlcNHglycan	17	21	PF01048	0.626
MOD_GlcNHglycan	173	176	PF01048	0.316
MOD_GlcNHglycan	260	263	PF01048	0.515
MOD_GlcNHglycan	324	327	PF01048	0.348
MOD_GlcNHglycan	346	349	PF01048	0.302
MOD_GlcNHglycan	405	408	PF01048	0.292
MOD_GlcNHglycan	463	467	PF01048	0.372
MOD_GSK3_1	138	145	PF00069	0.729
MOD_GSK3_1	150	157	PF00069	0.701
MOD_GSK3_1	158	165	PF00069	0.561
MOD_GSK3_1	171	178	PF00069	0.455
MOD_GSK3_1	181	188	PF00069	0.495
MOD_GSK3_1	258	265	PF00069	0.571
MOD_GSK3_1	269	276	PF00069	0.441
MOD_GSK3_1	318	325	PF00069	0.581
MOD_GSK3_1	403	410	PF00069	0.498
MOD_GSK3_1	431	438	PF00069	0.578
MOD_GSK3_1	455	462	PF00069	0.537
MOD_GSK3_1	467	474	PF00069	0.446
MOD_N-GLC_1	344	349	PF02516	0.318
MOD_N-GLC_1	403	408	PF02516	0.298
MOD_N-GLC_1	410	415	PF02516	0.298
MOD_NEK2_1	105	110	PF00069	0.573
MOD_NEK2_1	158	163	PF00069	0.643
MOD_NEK2_1	16	21	PF00069	0.557
MOD_NEK2_1	214	219	PF00069	0.490
MOD_NEK2_1	23	28	PF00069	0.418
MOD_NEK2_1	344	349	PF00069	0.531
MOD_NEK2_1	433	438	PF00069	0.498
MOD_NEK2_1	502	507	PF00069	0.510
MOD_NEK2_1	93	98	PF00069	0.707
MOD_PIKK_1	189	195	PF00454	0.604
MOD_PIKK_1	58	64	PF00454	0.488
MOD_PK_1	28	34	PF00069	0.384
MOD_PK_1	471	477	PF00069	0.498
MOD_PKA_2	166	172	PF00069	0.350
MOD_PKA_2	23	29	PF00069	0.476
MOD_PKA_2	242	248	PF00069	0.507
MOD_PKA_2	269	275	PF00069	0.548
MOD_PKA_2	431	437	PF00069	0.591
MOD_PKA_2	450	456	PF00069	0.498
MOD_PKA_2	470	476	PF00069	0.368
MOD_PKA_2	58	64	PF00069	0.468
MOD_PKA_2	88	94	PF00069	0.487
MOD_Plk_1	185	191	PF00069	0.498
MOD_Plk_1	386	392	PF00069	0.437
MOD_Plk_2-3	386	392	PF00069	0.437
MOD_Plk_2-3	515	521	PF00069	0.605
MOD_Plk_4	166	172	PF00069	0.350
MOD_Plk_4	88	94	PF00069	0.487
MOD_ProDKin_1	139	145	PF00069	0.746
MOD_ProDKin_1	351	357	PF00069	0.589
MOD_ProDKin_1	513	519	PF00069	0.592
MOD_ProDKin_1	520	526	PF00069	0.484
TRG_DiLeu_BaEn_2	289	295	PF01217	0.507
TRG_DiLeu_BaLyEn_6	125	130	PF01217	0.643
TRG_DiLeu_BaLyEn_6	380	385	PF01217	0.589
TRG_ENDOCYTIC_2	121	124	PF00928	0.643
TRG_ENDOCYTIC_2	126	129	PF00928	0.643
TRG_ENDOCYTIC_2	170	173	PF00928	0.498
TRG_ENDOCYTIC_2	293	296	PF00928	0.553
TRG_ER_diArg_1	492	494	PF00400	0.498
TRG_Pf-PMV_PEXEL_1	383	387	PF00026	0.298

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P6V5	Leptomonas seymouri	85%	100%
A0A0S4IX33	Bodo saltans	63%	100%
A0A1X0NKP6	Trypanosomatidae	75%	100%
A0A1X0NL55	Trypanosomatidae	75%	100%
A0A3R7R8D5	Trypanosoma rangeli	74%	100%
A0A3S5H5P8	Leishmania donovani	99%	100%
A4H4K2	Leishmania braziliensis	92%	100%
A4HSS8	Leishmania infantum	96%	100%
C9ZTD4	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	68%	100%
E9AKR5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	97%	100%
O22506	Daucus carota	46%	100%
P04773	Cricetulus griseus	42%	100%
P08281	Pisum sativum	43%	100%
P09606	Rattus norvegicus	44%	100%
P15102	Phaseolus vulgaris	45%	100%
P15103	Bos taurus	44%	100%
P15104	Homo sapiens	45%	100%
P15105	Mus musculus	45%	100%
P16580	Gallus gallus	44%	100%
P20477	Drosophila melanogaster	40%	100%
P32288	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	46%	100%
P34497	Caenorhabditis elegans	43%	100%
P38560	Zea mays	44%	100%
P46410	Sus scrofa	44%	100%
P51121	Xenopus laevis	46%	100%
Q12613	Colletotrichum gloeosporioides	46%	100%
Q42624	Brassica napus	44%	100%
Q42688	Chlamydomonas reinhardtii	44%	100%
Q42689	Chlamydomonas reinhardtii	42%	100%
Q43127	Arabidopsis thaliana	46%	100%
Q4R7U3	Macaca fascicularis	45%	100%
Q6B4U7	Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968)	42%	100%
Q6C3E0	Yarrowia lipolytica (strain CLIB 122 / E 150)	44%	100%
Q8HZM5	Canis lupus familiaris	45%	100%
Q9QY94	Acomys cahirinus	45%	100%
Q9XQ94	Medicago sativa	44%	100%
V5CHQ8	Trypanosoma cruzi	76%	100%