Putative ubiquitin activating E1 enzyme

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Ubiquitin-like modifier-activating enzyme ATG7, putative

Species:

Leishmania major

UniProt:

Q4QIU4_LEIMA

TriTrypDb:

LmjF.07.0010 , LMJLV39_070005100 , LMJSD75_070005100

Length:

725

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0000407	phagophore assembly site	2	2
GO:0005737	cytoplasm	2	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

Q4QIU4

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QIU4

Function

Biological processes
Term	Name	Level	Count
GO:0000045	autophagosome assembly	6	2
GO:0000422	autophagy of mitochondrion	4	2
GO:0006497	protein lipidation	5	2
GO:0006501	C-terminal protein lipidation	6	2
GO:0006807	nitrogen compound metabolic process	2	2
GO:0006914	autophagy	3	2
GO:0006950	response to stress	2	2
GO:0006995	cellular response to nitrogen starvation	5	2
GO:0006996	organelle organization	4	2
GO:0007005	mitochondrion organization	5	2
GO:0007033	vacuole organization	5	2
GO:0007154	cell communication	2	2
GO:0008152	metabolic process	1	2
GO:0009056	catabolic process	2	2
GO:0009267	cellular response to starvation	4	2
GO:0009605	response to external stimulus	2	2
GO:0009987	cellular process	1	2
GO:0009991	response to extracellular stimulus	3	2
GO:0016043	cellular component organization	3	2
GO:0016237	lysosomal microautophagy	4	2
GO:0018410	C-terminal protein amino acid modification	6	2
GO:0019538	protein metabolic process	3	2
GO:0022411	cellular component disassembly	4	2
GO:0022607	cellular component assembly	4	2
GO:0031667	response to nutrient levels	4	2
GO:0031668	cellular response to extracellular stimulus	3	2
GO:0031669	cellular response to nutrient levels	4	2
GO:0032446	protein modification by small protein conjugation	6	2
GO:0033554	cellular response to stress	3	2
GO:0034727	piecemeal microautophagy of the nucleus	5	2
GO:0036211	protein modification process	4	2
GO:0042594	response to starvation	3	2
GO:0043170	macromolecule metabolic process	3	2
GO:0043412	macromolecule modification	4	2
GO:0043562	cellular response to nitrogen levels	5	2
GO:0043687	post-translational protein modification	5	2
GO:0044237	cellular metabolic process	2	2
GO:0044238	primary metabolic process	2	2
GO:0044248	cellular catabolic process	3	2
GO:0044804	autophagy of nucleus	4	2
GO:0044805	late nucleophagy	5	2
GO:0050896	response to stimulus	1	2
GO:0051716	cellular response to stimulus	2	2
GO:0061726	mitochondrion disassembly	6	2
GO:0061919	process utilizing autophagic mechanism	2	2
GO:0070647	protein modification by small protein conjugation or removal	5	2
GO:0070925	organelle assembly	5	2
GO:0071496	cellular response to external stimulus	3	2
GO:0071704	organic substance metabolic process	2	2
GO:0071840	cellular component organization or biogenesis	2	2
GO:1901564	organonitrogen compound metabolic process	3	2
GO:1903008	organelle disassembly	5	2
GO:1905037	autophagosome organization	6	2

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	11
GO:0008641	ubiquitin-like modifier activating enzyme activity	2	11
GO:0016874	ligase activity	2	11
GO:0016877	ligase activity, forming carbon-sulfur bonds	3	11
GO:0019778	Atg12 activating enzyme activity	3	2
GO:0019779	Atg8 activating enzyme activity	3	2
GO:0140096	catalytic activity, acting on a protein	2	11
GO:0140657	ATP-dependent activity	1	11

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	135	139	PF00656	0.556
CLV_C14_Caspase3-7	152	156	PF00656	0.435
CLV_C14_Caspase3-7	243	247	PF00656	0.540
CLV_C14_Caspase3-7	445	449	PF00656	0.418
CLV_NRD_NRD_1	343	345	PF00675	0.294
CLV_NRD_NRD_1	490	492	PF00675	0.464
CLV_PCSK_KEX2_1	490	492	PF00082	0.412
CLV_PCSK_SKI1_1	111	115	PF00082	0.263
CLV_PCSK_SKI1_1	120	124	PF00082	0.240
CLV_PCSK_SKI1_1	28	32	PF00082	0.333
CLV_PCSK_SKI1_1	295	299	PF00082	0.333
CLV_PCSK_SKI1_1	300	304	PF00082	0.333
CLV_PCSK_SKI1_1	391	395	PF00082	0.356
CLV_PCSK_SKI1_1	406	410	PF00082	0.221
CLV_PCSK_SKI1_1	414	418	PF00082	0.298
CLV_PCSK_SKI1_1	55	59	PF00082	0.335
CLV_PCSK_SKI1_1	702	706	PF00082	0.406
DEG_APCC_DBOX_1	110	118	PF00400	0.505
DEG_APCC_DBOX_1	367	375	PF00400	0.401
DEG_APCC_DBOX_1	635	643	PF00400	0.539
DEG_COP1_1	506	517	PF00400	0.373
DEG_SIAH_1	458	466	PF03145	0.451
DOC_ANK_TNKS_1	539	546	PF00023	0.218
DOC_CKS1_1	562	567	PF01111	0.373
DOC_CYCLIN_RxL_1	25	34	PF00134	0.557
DOC_CYCLIN_RxL_1	388	399	PF00134	0.308
DOC_CYCLIN_yClb5_NLxxxL_5	105	114	PF00134	0.501
DOC_CYCLIN_yCln2_LP_2	122	128	PF00134	0.505
DOC_MAPK_DCC_7	375	384	PF00069	0.307
DOC_MAPK_gen_1	344	353	PF00069	0.591
DOC_MAPK_gen_1	375	384	PF00069	0.307
DOC_MAPK_HePTP_8	117	129	PF00069	0.501
DOC_MAPK_MEF2A_6	120	129	PF00069	0.454
DOC_MAPK_MEF2A_6	213	221	PF00069	0.492
DOC_MAPK_MEF2A_6	244	252	PF00069	0.557
DOC_MAPK_MEF2A_6	295	303	PF00069	0.533
DOC_MAPK_MEF2A_6	375	384	PF00069	0.307
DOC_MAPK_MEF2A_6	464	472	PF00069	0.356
DOC_PP1_RVXF_1	298	304	PF00149	0.534
DOC_PP1_RVXF_1	326	332	PF00149	0.456
DOC_PP1_RVXF_1	421	427	PF00149	0.393
DOC_PP2B_PxIxI_1	167	173	PF00149	0.446
DOC_PP2B_PxIxI_1	524	530	PF00149	0.451
DOC_PP4_FxxP_1	356	359	PF00568	0.486
DOC_PP4_FxxP_1	57	60	PF00568	0.501
DOC_PP4_FxxP_1	619	622	PF00568	0.384
DOC_USP7_MATH_1	171	175	PF00917	0.526
DOC_USP7_MATH_1	223	227	PF00917	0.529
DOC_USP7_MATH_1	236	240	PF00917	0.545
DOC_USP7_MATH_1	276	280	PF00917	0.433
DOC_USP7_MATH_1	287	291	PF00917	0.467
DOC_USP7_MATH_1	430	434	PF00917	0.418
DOC_USP7_MATH_1	50	54	PF00917	0.446
DOC_USP7_MATH_1	577	581	PF00917	0.307
DOC_USP7_MATH_1	595	599	PF00917	0.307
DOC_WW_Pin1_4	183	188	PF00397	0.484
DOC_WW_Pin1_4	230	235	PF00397	0.485
DOC_WW_Pin1_4	521	526	PF00397	0.418
DOC_WW_Pin1_4	56	61	PF00397	0.475
DOC_WW_Pin1_4	561	566	PF00397	0.373
DOC_WW_Pin1_4	688	693	PF00397	0.472
LIG_14-3-3_CanoR_1	201	207	PF00244	0.518
LIG_14-3-3_CanoR_1	267	277	PF00244	0.494
LIG_14-3-3_CanoR_1	421	427	PF00244	0.308
LIG_14-3-3_CanoR_1	431	435	PF00244	0.310
LIG_14-3-3_CanoR_1	49	58	PF00244	0.522
LIG_14-3-3_CanoR_1	594	604	PF00244	0.379
LIG_Actin_WH2_2	273	291	PF00022	0.535
LIG_Actin_WH2_2	98	113	PF00022	0.501
LIG_BIR_II_1	1	5	PF00653	0.702
LIG_BIR_III_4	138	142	PF00653	0.501
LIG_BIR_III_4	713	717	PF00653	0.578
LIG_BRCT_BRCA1_1	225	229	PF00533	0.557
LIG_BRCT_BRCA1_1	579	583	PF00533	0.418
LIG_BRCT_BRCA1_1	690	694	PF00533	0.437
LIG_BRCT_BRCA1_1	97	101	PF00533	0.501
LIG_Clathr_ClatBox_1	434	438	PF01394	0.418
LIG_deltaCOP1_diTrp_1	138	145	PF00928	0.458
LIG_EH_1	215	219	PF12763	0.453
LIG_EH_1	662	666	PF12763	0.477
LIG_FHA_1	202	208	PF00498	0.473
LIG_FHA_1	233	239	PF00498	0.501
LIG_FHA_1	280	286	PF00498	0.501
LIG_FHA_1	395	401	PF00498	0.379
LIG_FHA_1	522	528	PF00498	0.411
LIG_FHA_1	533	539	PF00498	0.318
LIG_FHA_1	576	582	PF00498	0.418
LIG_FHA_2	150	156	PF00498	0.480
LIG_FHA_2	241	247	PF00498	0.501
LIG_FHA_2	415	421	PF00498	0.307
LIG_FHA_2	443	449	PF00498	0.411
LIG_FHA_2	9	15	PF00498	0.435
LIG_GBD_Chelix_1	310	318	PF00786	0.357
LIG_GBD_Chelix_1	600	608	PF00786	0.379
LIG_LIR_Apic_2	54	60	PF02991	0.501
LIG_LIR_Apic_2	611	616	PF02991	0.418
LIG_LIR_Apic_2	617	622	PF02991	0.496
LIG_LIR_Gen_1	11	19	PF02991	0.543
LIG_LIR_Gen_1	226	237	PF02991	0.493
LIG_LIR_Gen_1	296	306	PF02991	0.495
LIG_LIR_Gen_1	322	329	PF02991	0.559
LIG_LIR_Gen_1	425	434	PF02991	0.412
LIG_LIR_Gen_1	534	544	PF02991	0.307
LIG_LIR_Nem_3	11	15	PF02991	0.489
LIG_LIR_Nem_3	158	162	PF02991	0.404
LIG_LIR_Nem_3	165	170	PF02991	0.471
LIG_LIR_Nem_3	226	232	PF02991	0.484
LIG_LIR_Nem_3	296	301	PF02991	0.504
LIG_LIR_Nem_3	322	326	PF02991	0.541
LIG_LIR_Nem_3	333	338	PF02991	0.496
LIG_LIR_Nem_3	425	429	PF02991	0.412
LIG_LIR_Nem_3	580	586	PF02991	0.418
LIG_PAM2_1	334	346	PF00658	0.557
LIG_Pex14_1	331	335	PF04695	0.533
LIG_Rb_pABgroove_1	247	255	PF01858	0.501
LIG_SH2_GRB2like	222	225	PF00017	0.362
LIG_SH2_NCK_1	130	134	PF00017	0.501
LIG_SH2_SRC	153	156	PF00017	0.533
LIG_SH2_SRC	613	616	PF00017	0.418
LIG_SH2_STAT5	153	156	PF00017	0.498
LIG_SH2_STAT5	536	539	PF00017	0.290
LIG_SH2_STAT5	569	572	PF00017	0.312
LIG_SH3_3	184	190	PF00018	0.550
LIG_SH3_3	259	265	PF00018	0.499
LIG_SH3_3	459	465	PF00018	0.415
LIG_SH3_3	589	595	PF00018	0.401
LIG_SH3_3	686	692	PF00018	0.401
LIG_SUMO_SIM_anti_2	415	420	PF11976	0.314
LIG_TRAF2_1	132	135	PF00917	0.531
LIG_TRAF2_1	305	308	PF00917	0.528
LIG_TRAF2_1	719	722	PF00917	0.624
LIG_TRAF2_2	359	364	PF00917	0.497
LIG_UBA3_1	114	120	PF00899	0.499
LIG_UBA3_1	150	157	PF00899	0.557
LIG_WRC_WIRS_1	229	234	PF05994	0.505
LIG_WRC_WIRS_1	434	439	PF05994	0.418
LIG_WRC_WIRS_1	96	101	PF05994	0.535
MOD_CK1_1	2	8	PF00069	0.617
MOD_CK1_1	278	284	PF00069	0.506
MOD_CK1_1	290	296	PF00069	0.504
MOD_CK1_1	309	315	PF00069	0.330
MOD_CK1_1	433	439	PF00069	0.414
MOD_CK1_1	582	588	PF00069	0.356
MOD_CK1_1	599	605	PF00069	0.393
MOD_CK1_1	649	655	PF00069	0.441
MOD_CK1_1	67	73	PF00069	0.543
MOD_CK1_1	74	80	PF00069	0.546
MOD_CK2_1	129	135	PF00069	0.566
MOD_CK2_1	310	316	PF00069	0.517
MOD_CK2_1	582	588	PF00069	0.442
MOD_CK2_1	690	696	PF00069	0.427
MOD_CK2_1	715	721	PF00069	0.652
MOD_GlcNHglycan	1	4	PF01048	0.634
MOD_GlcNHglycan	131	134	PF01048	0.332
MOD_GlcNHglycan	173	176	PF01048	0.307
MOD_GlcNHglycan	191	194	PF01048	0.320
MOD_GlcNHglycan	238	241	PF01048	0.306
MOD_GlcNHglycan	289	292	PF01048	0.368
MOD_GlcNHglycan	340	343	PF01048	0.284
MOD_GlcNHglycan	459	462	PF01048	0.451
MOD_GlcNHglycan	601	604	PF01048	0.322
MOD_GlcNHglycan	616	619	PF01048	0.468
MOD_GlcNHglycan	66	69	PF01048	0.301
MOD_GSK3_1	228	235	PF00069	0.545
MOD_GSK3_1	236	243	PF00069	0.530
MOD_GSK3_1	275	282	PF00069	0.390
MOD_GSK3_1	306	313	PF00069	0.548
MOD_GSK3_1	334	341	PF00069	0.480
MOD_GSK3_1	575	582	PF00069	0.403
MOD_GSK3_1	595	602	PF00069	0.121
MOD_GSK3_1	67	74	PF00069	0.552
MOD_N-GLC_1	223	228	PF02516	0.357
MOD_N-GLC_1	364	369	PF02516	0.363
MOD_N-GLC_1	50	55	PF02516	0.357
MOD_NEK2_1	255	260	PF00069	0.501
MOD_NEK2_1	275	280	PF00069	0.321
MOD_NEK2_1	306	311	PF00069	0.504
MOD_NEK2_1	338	343	PF00069	0.525
MOD_NEK2_1	394	399	PF00069	0.401
MOD_NEK2_1	651	656	PF00069	0.526
MOD_NEK2_2	430	435	PF00069	0.418
MOD_NEK2_2	558	563	PF00069	0.451
MOD_PIKK_1	74	80	PF00454	0.473
MOD_PKA_2	316	322	PF00069	0.558
MOD_PKA_2	422	428	PF00069	0.349
MOD_PKA_2	430	436	PF00069	0.262
MOD_PKA_2	558	564	PF00069	0.455
MOD_PKA_2	646	652	PF00069	0.376
MOD_Plk_1	223	229	PF00069	0.558
MOD_Plk_1	293	299	PF00069	0.467
MOD_Plk_1	306	312	PF00069	0.467
MOD_Plk_1	364	370	PF00069	0.362
MOD_Plk_1	414	420	PF00069	0.386
MOD_Plk_4	118	124	PF00069	0.514
MOD_Plk_4	149	155	PF00069	0.498
MOD_Plk_4	202	208	PF00069	0.519
MOD_Plk_4	293	299	PF00069	0.478
MOD_Plk_4	306	312	PF00069	0.467
MOD_Plk_4	414	420	PF00069	0.372
MOD_Plk_4	430	436	PF00069	0.241
MOD_Plk_4	513	519	PF00069	0.307
MOD_Plk_4	532	538	PF00069	0.307
MOD_Plk_4	646	652	PF00069	0.379
MOD_Plk_4	690	696	PF00069	0.434
MOD_ProDKin_1	183	189	PF00069	0.484
MOD_ProDKin_1	230	236	PF00069	0.485
MOD_ProDKin_1	521	527	PF00069	0.418
MOD_ProDKin_1	56	62	PF00069	0.475
MOD_ProDKin_1	561	567	PF00069	0.373
MOD_ProDKin_1	688	694	PF00069	0.470
MOD_SUMO_for_1	706	709	PF00179	0.578
MOD_SUMO_rev_2	438	445	PF00179	0.456
TRG_DiLeu_BaEn_1	173	178	PF01217	0.557
TRG_DiLeu_BaEn_2	293	299	PF01217	0.533
TRG_DiLeu_BaEn_4	103	109	PF01217	0.557
TRG_DiLeu_BaEn_4	333	339	PF01217	0.557
TRG_ENDOCYTIC_2	159	162	PF00928	0.473
TRG_ENDOCYTIC_2	536	539	PF00928	0.307
TRG_ER_diArg_1	421	424	PF00400	0.451
TRG_ER_diArg_1	489	491	PF00400	0.412
TRG_ER_diArg_1	636	639	PF00400	0.560
TRG_NLS_MonoCore_2	343	348	PF00514	0.533
TRG_NLS_MonoExtC_3	343	349	PF00514	0.533
TRG_Pf-PMV_PEXEL_1	484	488	PF00026	0.451
TRG_Pf-PMV_PEXEL_1	490	494	PF00026	0.352
TRG_Pf-PMV_PEXEL_1	584	588	PF00026	0.307

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P2V7	Leptomonas seymouri	65%	100%
A0A1X0P0S1	Trypanosomatidae	37%	88%
A0A3R7K2D8	Trypanosoma rangeli	41%	95%
A0A3S5H5V4	Leishmania donovani	96%	100%
A3LPA1	Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545)	30%	100%
A4H4U4	Leishmania braziliensis	84%	100%
A4HT22	Leishmania infantum	96%	100%
A5DLC6	Meyerozyma guilliermondii (strain ATCC 6260 / CBS 566 / DSM 6381 / JCM 1539 / NBRC 10279 / NRRL Y-324)	31%	100%
A5E0T7	Lodderomyces elongisporus (strain ATCC 11503 / CBS 2605 / JCM 1781 / NBRC 1676 / NRRL YB-4239)	31%	100%
A6QXC6	Ajellomyces capsulatus (strain NAm1 / WU24)	32%	100%
A6ZT79	Saccharomyces cerevisiae (strain YJM789)	31%	100%
A7EI75	Sclerotinia sclerotiorum (strain ATCC 18683 / 1980 / Ss-1)	31%	100%
A7KAI6	Pichia angusta	30%	100%
A7KAL8	Penicillium rubens (strain ATCC 28089 / DSM 1075 / NRRL 1951 / Wisconsin 54-1255)	31%	100%
A7TEY0	Vanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294 / BCRC 21397 / CBS 2163 / NBRC 10782 / NRRL Y-8283 / UCD 57-17)	30%	100%
D0A4R0	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	41%	96%
E9AL10	Leishmania mexicana (strain MHOM/GT/2001/U1103)	95%	100%
F7W4M2	Sordaria macrospora (strain ATCC MYA-333 / DSM 997 / K(L3346) / K-hell)	32%	100%
G2XR75	Botryotinia fuckeliana (strain T4)	30%	100%
I1S0J7	Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1)	34%	100%
M7U9B9	Botryotinia fuckeliana (strain BcDW1)	30%	95%
O43069	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	31%	100%
O93922	Komagataella pastoris	30%	100%
O95352	Homo sapiens	31%	100%
P0CM38	Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565)	32%	100%
P0CM39	Cryptococcus neoformans var. neoformans serotype D (strain B-3501A)	32%	100%
P38862	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	31%	100%
Q52CS0	Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958)	30%	100%
Q5AWA2	Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139)	34%	100%
Q5ZKY2	Gallus gallus	33%	100%
Q641Y5	Rattus norvegicus	32%	100%
Q6BGV9	Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968)	30%	100%
Q6CBC3	Yarrowia lipolytica (strain CLIB 122 / E 150)	31%	100%
Q6FQY7	Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65)	29%	100%
Q86CR9	Dictyostelium discoideum	31%	100%
Q871U2	Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)	31%	100%
Q94CD5	Arabidopsis thaliana	32%	100%
Q9D906	Mus musculus	33%	100%
V5B6Z1	Trypanosoma cruzi	41%	95%