Putative tRNA-methyl transferase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

tRNA-methyl transferase, putative

Species:

Leishmania major

UniProt:

Q4QIT1_LEIMA

TriTrypDb:

LmjF.07.0130 , LMJLV39_070006800 , LMJSD75_070006800

Length:

603

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Could not find GO cellular_component term for this entry.

Expansion

Sequence features

Q4QIT1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QIT1

Function

Biological processes
Term	Name	Level	Count
GO:0002097	tRNA wobble base modification	7	2
GO:0002098	tRNA wobble uridine modification	8	2
GO:0002143	tRNA wobble position uridine thiolation	8	2
GO:0006139	nucleobase-containing compound metabolic process	3	2
GO:0006396	RNA processing	6	2
GO:0006399	tRNA metabolic process	7	2
GO:0006400	tRNA modification	6	2
GO:0006725	cellular aromatic compound metabolic process	3	2
GO:0006807	nitrogen compound metabolic process	2	2
GO:0008033	tRNA processing	8	2
GO:0008152	metabolic process	1	2
GO:0009451	RNA modification	5	2
GO:0009987	cellular process	1	2
GO:0016070	RNA metabolic process	5	2
GO:0034227	tRNA thio-modification	7	2
GO:0034470	ncRNA processing	7	2
GO:0034641	cellular nitrogen compound metabolic process	3	2
GO:0034660	ncRNA metabolic process	6	2
GO:0043170	macromolecule metabolic process	3	2
GO:0043412	macromolecule modification	4	2
GO:0044237	cellular metabolic process	2	2
GO:0044238	primary metabolic process	2	2
GO:0046483	heterocycle metabolic process	3	2
GO:0071704	organic substance metabolic process	2	2
GO:0090304	nucleic acid metabolic process	4	2
GO:1901360	organic cyclic compound metabolic process	3	2

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	10
GO:0004808	tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase activity	6	8
GO:0008168	methyltransferase activity	4	8
GO:0008173	RNA methyltransferase activity	4	8
GO:0008175	tRNA methyltransferase activity	5	8
GO:0008757	S-adenosylmethionine-dependent methyltransferase activity	5	8
GO:0016740	transferase activity	2	10
GO:0016741	transferase activity, transferring one-carbon groups	3	8
GO:0140098	catalytic activity, acting on RNA	3	8
GO:0140101	catalytic activity, acting on a tRNA	4	8
GO:0140640	catalytic activity, acting on a nucleic acid	2	8

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	575	579	PF00656	0.766
CLV_NRD_NRD_1	187	189	PF00675	0.359
CLV_NRD_NRD_1	221	223	PF00675	0.334
CLV_NRD_NRD_1	300	302	PF00675	0.516
CLV_NRD_NRD_1	415	417	PF00675	0.547
CLV_NRD_NRD_1	536	538	PF00675	0.304
CLV_PCSK_FUR_1	534	538	PF00082	0.304
CLV_PCSK_KEX2_1	187	189	PF00082	0.359
CLV_PCSK_KEX2_1	220	222	PF00082	0.304
CLV_PCSK_KEX2_1	415	417	PF00082	0.579
CLV_PCSK_KEX2_1	536	538	PF00082	0.304
CLV_PCSK_PC1ET2_1	220	222	PF00082	0.304
CLV_PCSK_SKI1_1	222	226	PF00082	0.306
CLV_PCSK_SKI1_1	349	353	PF00082	0.516
CLV_PCSK_SKI1_1	547	551	PF00082	0.280
CLV_PCSK_SKI1_1	74	78	PF00082	0.604
DOC_ANK_TNKS_1	414	421	PF00023	0.549
DOC_CKS1_1	94	99	PF01111	0.559
DOC_CYCLIN_RxL_1	125	136	PF00134	0.508
DOC_CYCLIN_yCln2_LP_2	399	405	PF00134	0.419
DOC_CYCLIN_yCln2_LP_2	481	487	PF00134	0.524
DOC_MAPK_DCC_7	49	58	PF00069	0.333
DOC_MAPK_gen_1	220	227	PF00069	0.504
DOC_MAPK_gen_1	301	310	PF00069	0.485
DOC_MAPK_gen_1	534	542	PF00069	0.559
DOC_MAPK_MEF2A_6	220	227	PF00069	0.504
DOC_MAPK_MEF2A_6	49	58	PF00069	0.333
DOC_MAPK_MEF2A_6	7	16	PF00069	0.534
DOC_PP1_RVXF_1	174	180	PF00149	0.535
DOC_PP1_RVXF_1	537	543	PF00149	0.559
DOC_PP2B_LxvP_1	337	340	PF13499	0.591
DOC_PP2B_LxvP_1	361	364	PF13499	0.579
DOC_PP2B_LxvP_1	481	484	PF13499	0.533
DOC_PP4_FxxP_1	310	313	PF00568	0.622
DOC_PP4_FxxP_1	45	48	PF00568	0.592
DOC_PP4_FxxP_1	542	545	PF00568	0.456
DOC_PP4_FxxP_1	600	603	PF00568	0.386
DOC_USP7_MATH_1	272	276	PF00917	0.535
DOC_USP7_MATH_1	284	288	PF00917	0.694
DOC_USP7_MATH_1	372	376	PF00917	0.515
DOC_USP7_UBL2_3	565	569	PF12436	0.412
DOC_WW_Pin1_4	135	140	PF00397	0.559
DOC_WW_Pin1_4	547	552	PF00397	0.492
DOC_WW_Pin1_4	579	584	PF00397	0.699
DOC_WW_Pin1_4	594	599	PF00397	0.573
DOC_WW_Pin1_4	93	98	PF00397	0.555
LIG_14-3-3_CanoR_1	228	236	PF00244	0.475
LIG_14-3-3_CanoR_1	273	277	PF00244	0.570
LIG_14-3-3_CanoR_1	301	311	PF00244	0.492
LIG_14-3-3_CanoR_1	31	39	PF00244	0.424
LIG_14-3-3_CanoR_1	415	419	PF00244	0.549
LIG_Actin_WH2_2	552	567	PF00022	0.445
LIG_APCC_ABBAyCdc20_2	537	543	PF00400	0.531
LIG_BIR_II_1	1	5	PF00653	0.630
LIG_BIR_III_2	578	582	PF00653	0.759
LIG_BIR_III_2	89	93	PF00653	0.531
LIG_BRCT_BRCA1_1	325	329	PF00533	0.635
LIG_BRCT_BRCA1_1	596	600	PF00533	0.361
LIG_CSL_BTD_1	479	482	PF09270	0.465
LIG_EVH1_1	337	341	PF00568	0.558
LIG_FHA_1	127	133	PF00498	0.511
LIG_FHA_1	164	170	PF00498	0.469
LIG_FHA_1	181	187	PF00498	0.456
LIG_FHA_1	204	210	PF00498	0.486
LIG_FHA_1	23	29	PF00498	0.442
LIG_FHA_1	238	244	PF00498	0.438
LIG_FHA_1	280	286	PF00498	0.733
LIG_FHA_1	34	40	PF00498	0.431
LIG_FHA_1	346	352	PF00498	0.480
LIG_FHA_2	33	39	PF00498	0.425
LIG_FHA_2	355	361	PF00498	0.492
LIG_FHA_2	75	81	PF00498	0.580
LIG_LIR_Apic_2	206	210	PF02991	0.504
LIG_LIR_Apic_2	335	339	PF02991	0.514
LIG_LIR_Apic_2	476	482	PF02991	0.473
LIG_LIR_Apic_2	597	603	PF02991	0.446
LIG_LIR_Gen_1	151	162	PF02991	0.485
LIG_LIR_Nem_3	326	332	PF02991	0.593
LIG_LIR_Nem_3	395	399	PF02991	0.575
LIG_LYPXL_SIV_4	426	434	PF13949	0.435
LIG_LYPXL_yS_3	396	399	PF13949	0.493
LIG_MYND_1	397	401	PF01753	0.468
LIG_MYND_3	559	563	PF01753	0.450
LIG_Pex14_2	99	103	PF04695	0.456
LIG_REV1ctd_RIR_1	96	105	PF16727	0.504
LIG_SH2_CRK	384	388	PF00017	0.476
LIG_SH2_CRK	558	562	PF00017	0.438
LIG_SH2_NCK_1	120	124	PF00017	0.486
LIG_SH2_NCK_1	88	92	PF00017	0.504
LIG_SH2_SRC	120	123	PF00017	0.535
LIG_SH2_SRC	336	339	PF00017	0.580
LIG_SH2_STAP1	203	207	PF00017	0.456
LIG_SH2_STAP1	403	407	PF00017	0.392
LIG_SH2_STAP1	468	472	PF00017	0.497
LIG_SH2_STAT3	149	152	PF00017	0.535
LIG_SH2_STAT3	468	471	PF00017	0.525
LIG_SH2_STAT5	149	152	PF00017	0.439
LIG_SH2_STAT5	336	339	PF00017	0.542
LIG_SH2_STAT5	428	431	PF00017	0.362
LIG_SH2_STAT5	468	471	PF00017	0.506
LIG_SH3_1	336	342	PF00018	0.639
LIG_SH3_3	308	314	PF00018	0.584
LIG_SH3_3	335	341	PF00018	0.623
LIG_SH3_3	391	397	PF00018	0.513
LIG_SH3_3	430	436	PF00018	0.529
LIG_SH3_3	45	51	PF00018	0.596
LIG_SH3_3	578	584	PF00018	0.741
LIG_SH3_3	592	598	PF00018	0.310
LIG_SUMO_SIM_anti_2	129	134	PF11976	0.448
LIG_SUMO_SIM_par_1	560	566	PF11976	0.521
LIG_TRAF2_1	357	360	PF00917	0.568
LIG_TRAF2_1	523	526	PF00917	0.559
LIG_TYR_ITIM	394	399	PF00017	0.367
LIG_TYR_ITIM	556	561	PF00017	0.350
LIG_UBA3_1	561	569	PF00899	0.520
MOD_CK1_1	107	113	PF00069	0.334
MOD_CK1_1	191	197	PF00069	0.453
MOD_CK1_1	2	8	PF00069	0.685
MOD_CK1_1	22	28	PF00069	0.144
MOD_CK1_1	279	285	PF00069	0.720
MOD_CK1_1	315	321	PF00069	0.644
MOD_CK1_1	324	330	PF00069	0.610
MOD_CK1_1	344	350	PF00069	0.328
MOD_CK1_1	375	381	PF00069	0.663
MOD_CK1_1	440	446	PF00069	0.650
MOD_CK1_1	470	476	PF00069	0.503
MOD_CK2_1	285	291	PF00069	0.609
MOD_CK2_1	354	360	PF00069	0.491
MOD_CK2_1	579	585	PF00069	0.644
MOD_CK2_1	74	80	PF00069	0.575
MOD_DYRK1A_RPxSP_1	547	551	PF00069	0.373
MOD_GlcNHglycan	105	109	PF01048	0.429
MOD_GlcNHglycan	16	19	PF01048	0.350
MOD_GlcNHglycan	21	24	PF01048	0.315
MOD_GlcNHglycan	230	233	PF01048	0.306
MOD_GlcNHglycan	248	251	PF01048	0.309
MOD_GlcNHglycan	278	281	PF01048	0.681
MOD_GlcNHglycan	314	317	PF01048	0.625
MOD_GlcNHglycan	343	346	PF01048	0.553
MOD_GlcNHglycan	378	381	PF01048	0.619
MOD_GlcNHglycan	439	442	PF01048	0.648
MOD_GlcNHglycan	524	530	PF01048	0.453
MOD_GlcNHglycan	591	594	PF01048	0.393
MOD_GSK3_1	193	200	PF00069	0.387
MOD_GSK3_1	237	244	PF00069	0.328
MOD_GSK3_1	246	253	PF00069	0.328
MOD_GSK3_1	272	279	PF00069	0.532
MOD_GSK3_1	297	304	PF00069	0.439
MOD_GSK3_1	320	327	PF00069	0.699
MOD_GSK3_1	341	348	PF00069	0.617
MOD_GSK3_1	372	379	PF00069	0.669
MOD_GSK3_1	466	473	PF00069	0.399
MOD_N-GLC_1	321	326	PF02516	0.589
MOD_NEK2_1	172	177	PF00069	0.418
MOD_NEK2_1	186	191	PF00069	0.243
MOD_NEK2_1	193	198	PF00069	0.329
MOD_NEK2_1	276	281	PF00069	0.599
MOD_NEK2_1	376	381	PF00069	0.477
MOD_NEK2_1	466	471	PF00069	0.414
MOD_OFUCOSY	319	325	PF10250	0.573
MOD_PIKK_1	467	473	PF00454	0.512
MOD_PIKK_1	5	11	PF00454	0.607
MOD_PK_1	188	194	PF00069	0.452
MOD_PKA_1	301	307	PF00069	0.475
MOD_PKA_2	186	192	PF00069	0.453
MOD_PKA_2	272	278	PF00069	0.513
MOD_PKA_2	414	420	PF00069	0.552
MOD_Plk_1	237	243	PF00069	0.309
MOD_Plk_4	188	194	PF00069	0.375
MOD_Plk_4	237	243	PF00069	0.317
MOD_ProDKin_1	135	141	PF00069	0.453
MOD_ProDKin_1	547	553	PF00069	0.360
MOD_ProDKin_1	579	585	PF00069	0.685
MOD_ProDKin_1	594	600	PF00069	0.575
MOD_ProDKin_1	93	99	PF00069	0.449
MOD_SUMO_rev_2	440	448	PF00179	0.646
TRG_DiLeu_BaLyEn_6	448	453	PF01217	0.450
TRG_DiLeu_BaLyEn_6	51	56	PF01217	0.330
TRG_DiLeu_BaLyEn_6	557	562	PF01217	0.428
TRG_ENDOCYTIC_2	396	399	PF00928	0.548
TRG_ENDOCYTIC_2	558	561	PF00928	0.356
TRG_ER_diArg_1	186	188	PF00400	0.453
TRG_ER_diArg_1	221	223	PF00400	0.469
TRG_ER_diArg_1	410	413	PF00400	0.504
TRG_ER_diArg_1	533	536	PF00400	0.418
TRG_ER_diArg_1	537	540	PF00400	0.423
TRG_Pf-PMV_PEXEL_1	234	238	PF00026	0.421
TRG_Pf-PMV_PEXEL_1	451	455	PF00026	0.489

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I0V4	Leptomonas seymouri	48%	100%
A0A1X0NK59	Trypanosomatidae	36%	100%
A0A3S7WPQ8	Leishmania donovani	93%	98%
A0A422NTD5	Trypanosoma rangeli	40%	100%
A4H4V6	Leishmania braziliensis	79%	100%
A4HT56	Leishmania infantum	93%	98%
C9ZUV8	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	36%	100%
E9AL23	Leishmania mexicana (strain MHOM/GT/2001/U1103)	88%	100%
Q1QBM4	Psychrobacter cryohalolentis (strain ATCC BAA-1226 / DSM 17306 / VKM B-2378 / K5)	27%	100%
Q4FSB4	Psychrobacter arcticus (strain DSM 17307 / VKM B-2377 / 273-4)	27%	100%
Q5ZKW0	Gallus gallus	26%	100%
V5BDF2	Trypanosoma cruzi	38%	100%