Ubiquitin-activating enzyme-like protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

ubiquitin-activating enzyme-like protein

Species:

Leishmania major

UniProt:

Q4QIE7_LEIMA

TriTrypDb:

LmjF.08.0220 , LMJLV39_080007200 , LMJSD75_080007200

Length:

837

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 7
NetGPI	no	yes: 0, no: 7

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	2
GO:0031510	SUMO activating enzyme complex	3	2
GO:0032991	protein-containing complex	1	2
GO:0110165	cellular anatomical entity	1	2
GO:0140513	nuclear protein-containing complex	2	2
GO:1902494	catalytic complex	2	2

Expansion

Sequence features

Q4QIE7

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QIE7

Function

Biological processes
Term	Name	Level	Count
GO:0006807	nitrogen compound metabolic process	2	8
GO:0008152	metabolic process	1	8
GO:0016925	protein sumoylation	7	8
GO:0018193	peptidyl-amino acid modification	5	8
GO:0018205	peptidyl-lysine modification	6	8
GO:0019538	protein metabolic process	3	8
GO:0032446	protein modification by small protein conjugation	6	8
GO:0036211	protein modification process	4	8
GO:0043170	macromolecule metabolic process	3	8
GO:0043412	macromolecule modification	4	8
GO:0044238	primary metabolic process	2	8
GO:0070647	protein modification by small protein conjugation or removal	5	8
GO:0071704	organic substance metabolic process	2	8
GO:1901564	organonitrogen compound metabolic process	3	8

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	8
GO:0003824	catalytic activity	1	8
GO:0005488	binding	1	8
GO:0005524	ATP binding	5	8
GO:0008641	ubiquitin-like modifier activating enzyme activity	2	8
GO:0016874	ligase activity	2	8
GO:0016877	ligase activity, forming carbon-sulfur bonds	3	8
GO:0017076	purine nucleotide binding	4	8
GO:0019948	SUMO activating enzyme activity	3	8
GO:0030554	adenyl nucleotide binding	5	8
GO:0032553	ribonucleotide binding	3	8
GO:0032555	purine ribonucleotide binding	4	8
GO:0032559	adenyl ribonucleotide binding	5	8
GO:0035639	purine ribonucleoside triphosphate binding	4	8
GO:0036094	small molecule binding	2	8
GO:0043167	ion binding	2	8
GO:0043168	anion binding	3	8
GO:0097159	organic cyclic compound binding	2	8
GO:0097367	carbohydrate derivative binding	2	8
GO:0140096	catalytic activity, acting on a protein	2	8
GO:0140657	ATP-dependent activity	1	8
GO:1901265	nucleoside phosphate binding	3	8
GO:1901363	heterocyclic compound binding	2	8

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	150	154	PF00656	0.481
CLV_C14_Caspase3-7	239	243	PF00656	0.481
CLV_C14_Caspase3-7	307	311	PF00656	0.256
CLV_C14_Caspase3-7	68	72	PF00656	0.350
CLV_NRD_NRD_1	119	121	PF00675	0.324
CLV_NRD_NRD_1	296	298	PF00675	0.479
CLV_NRD_NRD_1	361	363	PF00675	0.481
CLV_NRD_NRD_1	415	417	PF00675	0.384
CLV_NRD_NRD_1	522	524	PF00675	0.518
CLV_NRD_NRD_1	531	533	PF00675	0.573
CLV_NRD_NRD_1	95	97	PF00675	0.481
CLV_PCSK_FUR_1	117	121	PF00082	0.431
CLV_PCSK_FUR_1	529	533	PF00082	0.606
CLV_PCSK_KEX2_1	119	121	PF00082	0.324
CLV_PCSK_KEX2_1	295	297	PF00082	0.481
CLV_PCSK_KEX2_1	361	363	PF00082	0.481
CLV_PCSK_KEX2_1	529	531	PF00082	0.573
CLV_PCSK_KEX2_1	95	97	PF00082	0.481
CLV_PCSK_SKI1_1	189	193	PF00082	0.463
CLV_PCSK_SKI1_1	334	338	PF00082	0.432
CLV_PCSK_SKI1_1	36	40	PF00082	0.354
CLV_PCSK_SKI1_1	401	405	PF00082	0.429
CLV_PCSK_SKI1_1	77	81	PF00082	0.343
CLV_PCSK_SKI1_1	96	100	PF00082	0.350
CLV_Separin_Metazoa	331	335	PF03568	0.431
CLV_Separin_Metazoa	689	693	PF03568	0.504
DEG_APCC_DBOX_1	76	84	PF00400	0.432
DEG_SPOP_SBC_1	300	304	PF00917	0.282
DEG_SPOP_SBC_1	486	490	PF00917	0.785
DEG_SPOP_SBC_1	795	799	PF00917	0.583
DOC_CYCLIN_RxL_1	331	339	PF00134	0.481
DOC_CYCLIN_RxL_1	49	60	PF00134	0.384
DOC_MAPK_gen_1	529	535	PF00069	0.710
DOC_MAPK_JIP1_4	409	415	PF00069	0.481
DOC_MAPK_MEF2A_6	540	548	PF00069	0.759
DOC_PP1_RVXF_1	332	338	PF00149	0.481
DOC_PP2B_LxvP_1	661	664	PF13499	0.589
DOC_PP2B_LxvP_1	691	694	PF13499	0.589
DOC_PP2B_LxvP_1	782	785	PF13499	0.769
DOC_USP7_MATH_1	16	20	PF00917	0.658
DOC_USP7_MATH_1	300	304	PF00917	0.352
DOC_USP7_MATH_1	370	374	PF00917	0.407
DOC_USP7_MATH_1	421	425	PF00917	0.451
DOC_USP7_MATH_1	440	444	PF00917	0.200
DOC_USP7_MATH_1	487	491	PF00917	0.787
DOC_USP7_MATH_1	507	511	PF00917	0.463
DOC_USP7_MATH_1	558	562	PF00917	0.797
DOC_USP7_MATH_1	581	585	PF00917	0.671
DOC_USP7_MATH_1	6	10	PF00917	0.750
DOC_USP7_MATH_1	601	605	PF00917	0.413
DOC_USP7_MATH_1	664	668	PF00917	0.547
DOC_USP7_MATH_1	737	741	PF00917	0.556
DOC_WW_Pin1_4	11	16	PF00397	0.780
DOC_WW_Pin1_4	118	123	PF00397	0.484
DOC_WW_Pin1_4	19	24	PF00397	0.697
DOC_WW_Pin1_4	511	516	PF00397	0.723
DOC_WW_Pin1_4	533	538	PF00397	0.700
DOC_WW_Pin1_4	540	545	PF00397	0.689
DOC_WW_Pin1_4	560	565	PF00397	0.473
DOC_WW_Pin1_4	735	740	PF00397	0.695
DOC_WW_Pin1_4	797	802	PF00397	0.585
DOC_WW_Pin1_4	815	820	PF00397	0.631
LIG_14-3-3_CanoR_1	18	25	PF00244	0.590
LIG_14-3-3_CanoR_1	215	220	PF00244	0.350
LIG_14-3-3_CanoR_1	321	328	PF00244	0.411
LIG_14-3-3_CanoR_1	401	410	PF00244	0.431
LIG_14-3-3_CanoR_1	478	486	PF00244	0.481
LIG_14-3-3_CanoR_1	540	544	PF00244	0.735
LIG_14-3-3_CanoR_1	583	591	PF00244	0.633
LIG_14-3-3_CanoR_1	637	641	PF00244	0.445
LIG_14-3-3_CanoR_1	643	648	PF00244	0.550
LIG_14-3-3_CanoR_1	705	711	PF00244	0.558
LIG_Actin_WH2_2	462	480	PF00022	0.481
LIG_BIR_II_1	1	5	PF00653	0.500
LIG_BIR_III_1	1	5	PF00653	0.500
LIG_BIR_III_3	1	5	PF00653	0.500
LIG_BRCT_BRCA1_1	739	743	PF00533	0.621
LIG_Clathr_ClatBox_1	64	68	PF01394	0.432
LIG_FHA_1	204	210	PF00498	0.333
LIG_FHA_1	215	221	PF00498	0.294
LIG_FHA_1	402	408	PF00498	0.416
LIG_FHA_1	421	427	PF00498	0.221
LIG_FHA_1	541	547	PF00498	0.705
LIG_FHA_1	583	589	PF00498	0.669
LIG_FHA_1	637	643	PF00498	0.390
LIG_FHA_1	786	792	PF00498	0.760
LIG_FHA_1	797	803	PF00498	0.741
LIG_FHA_2	337	343	PF00498	0.497
LIG_FHA_2	724	730	PF00498	0.562
LIG_GBD_Chelix_1	328	336	PF00786	0.432
LIG_GBD_Chelix_1	469	477	PF00786	0.432
LIG_LIR_Gen_1	228	234	PF02991	0.339
LIG_LIR_Gen_1	57	67	PF02991	0.432
LIG_LIR_Gen_1	676	687	PF02991	0.522
LIG_LIR_Gen_1	718	725	PF02991	0.553
LIG_LIR_Nem_3	139	145	PF02991	0.384
LIG_LIR_Nem_3	228	233	PF02991	0.339
LIG_LIR_Nem_3	57	62	PF02991	0.432
LIG_LIR_Nem_3	676	682	PF02991	0.497
LIG_LIR_Nem_3	718	724	PF02991	0.519
LIG_LIR_Nem_3	740	746	PF02991	0.592
LIG_MAD2	518	526	PF02301	0.419
LIG_NRBOX	331	337	PF00104	0.481
LIG_NRBOX	472	478	PF00104	0.432
LIG_Pex14_2	141	145	PF04695	0.334
LIG_SH2_NCK_1	137	141	PF00017	0.384
LIG_SH2_SRC	137	140	PF00017	0.432
LIG_SH2_STAP1	137	141	PF00017	0.384
LIG_SH2_STAP1	225	229	PF00017	0.325
LIG_SH2_STAP1	230	234	PF00017	0.325
LIG_SH2_STAP1	521	525	PF00017	0.576
LIG_SH2_STAP1	679	683	PF00017	0.553
LIG_SH2_STAP1	708	712	PF00017	0.589
LIG_SH2_STAT3	180	183	PF00017	0.350
LIG_SH2_STAT3	195	198	PF00017	0.350
LIG_SH2_STAT5	286	289	PF00017	0.432
LIG_SH2_STAT5	550	553	PF00017	0.709
LIG_SH2_STAT5	587	590	PF00017	0.657
LIG_SH2_STAT5	611	614	PF00017	0.523
LIG_SH2_STAT5	708	711	PF00017	0.589
LIG_SH3_1	531	537	PF00018	0.727
LIG_SH3_2	618	623	PF14604	0.556
LIG_SH3_3	196	202	PF00018	0.432
LIG_SH3_3	2	8	PF00018	0.753
LIG_SH3_3	22	28	PF00018	0.591
LIG_SH3_3	349	355	PF00018	0.354
LIG_SH3_3	531	537	PF00018	0.720
LIG_SH3_3	615	621	PF00018	0.483
LIG_SUMO_SIM_anti_2	472	482	PF11976	0.432
LIG_SUMO_SIM_anti_2	604	609	PF11976	0.649
LIG_SUMO_SIM_anti_2	831	837	PF11976	0.715
LIG_SUMO_SIM_par_1	342	350	PF11976	0.384
LIG_SUMO_SIM_par_1	387	394	PF11976	0.394
LIG_SUMO_SIM_par_1	52	57	PF11976	0.373
LIG_SUMO_SIM_par_1	63	69	PF11976	0.327
LIG_SUMO_SIM_par_1	638	646	PF11976	0.589
LIG_SUMO_SIM_par_1	797	805	PF11976	0.711
LIG_SUMO_SIM_par_1	831	837	PF11976	0.715
LIG_SxIP_EBH_1	448	461	PF03271	0.481
LIG_TRAF2_1	754	757	PF00917	0.615
LIG_TRAF2_1	768	771	PF00917	0.539
LIG_UBA3_1	332	340	PF00899	0.392
LIG_UBA3_1	344	348	PF00899	0.290
MOD_CDK_SPK_2	511	516	PF00069	0.723
MOD_CDK_SPxxK_3	11	18	PF00069	0.801
MOD_CDK_SPxxK_3	511	518	PF00069	0.495
MOD_CDK_SPxxK_3	533	540	PF00069	0.588
MOD_CK1_1	14	20	PF00069	0.787
MOD_CK1_1	236	242	PF00069	0.438
MOD_CK1_1	247	253	PF00069	0.446
MOD_CK1_1	320	326	PF00069	0.458
MOD_CK1_1	346	352	PF00069	0.356
MOD_CK1_1	424	430	PF00069	0.481
MOD_CK1_1	479	485	PF00069	0.432
MOD_CK1_1	510	516	PF00069	0.706
MOD_CK1_1	57	63	PF00069	0.432
MOD_CK1_1	738	744	PF00069	0.683
MOD_CK1_1	794	800	PF00069	0.767
MOD_CK2_1	312	318	PF00069	0.481
MOD_CK2_1	336	342	PF00069	0.432
MOD_CK2_1	495	501	PF00069	0.787
MOD_CK2_1	554	560	PF00069	0.737
MOD_CK2_1	744	750	PF00069	0.567
MOD_Cter_Amidation	117	120	PF01082	0.431
MOD_GlcNHglycan	113	116	PF01048	0.332
MOD_GlcNHglycan	149	152	PF01048	0.411
MOD_GlcNHglycan	155	158	PF01048	0.352
MOD_GlcNHglycan	174	177	PF01048	0.207
MOD_GlcNHglycan	246	249	PF01048	0.418
MOD_GlcNHglycan	261	264	PF01048	0.444
MOD_GlcNHglycan	312	317	PF01048	0.460
MOD_GlcNHglycan	372	375	PF01048	0.434
MOD_GlcNHglycan	380	383	PF01048	0.346
MOD_GlcNHglycan	384	387	PF01048	0.302
MOD_GlcNHglycan	426	429	PF01048	0.432
MOD_GlcNHglycan	489	492	PF01048	0.744
MOD_GlcNHglycan	504	508	PF01048	0.549
MOD_GlcNHglycan	556	559	PF01048	0.810
MOD_GlcNHglycan	56	59	PF01048	0.432
MOD_GlcNHglycan	564	567	PF01048	0.655
MOD_GlcNHglycan	591	594	PF01048	0.654
MOD_GlcNHglycan	656	659	PF01048	0.352
MOD_GlcNHglycan	746	749	PF01048	0.555
MOD_GlcNHglycan	793	796	PF01048	0.774
MOD_GSK3_1	282	289	PF00069	0.473
MOD_GSK3_1	343	350	PF00069	0.346
MOD_GSK3_1	370	377	PF00069	0.447
MOD_GSK3_1	378	385	PF00069	0.417
MOD_GSK3_1	420	427	PF00069	0.491
MOD_GSK3_1	446	453	PF00069	0.406
MOD_GSK3_1	457	464	PF00069	0.432
MOD_GSK3_1	472	479	PF00069	0.238
MOD_GSK3_1	481	488	PF00069	0.337
MOD_GSK3_1	503	510	PF00069	0.742
MOD_GSK3_1	554	561	PF00069	0.778
MOD_GSK3_1	66	73	PF00069	0.350
MOD_GSK3_1	664	671	PF00069	0.426
MOD_GSK3_1	7	14	PF00069	0.732
MOD_GSK3_1	704	711	PF00069	0.444
MOD_GSK3_1	719	726	PF00069	0.275
MOD_GSK3_1	786	793	PF00069	0.791
MOD_GSK3_1	86	93	PF00069	0.157
MOD_LATS_1	213	219	PF00433	0.384
MOD_N-GLC_1	153	158	PF02516	0.373
MOD_N-GLC_1	259	264	PF02516	0.256
MOD_N-GLC_1	374	379	PF02516	0.465
MOD_N-GLC_1	461	466	PF02516	0.497
MOD_N-GLC_1	558	563	PF02516	0.777
MOD_NEK2_1	172	177	PF00069	0.346
MOD_NEK2_1	336	341	PF00069	0.429
MOD_NEK2_1	347	352	PF00069	0.347
MOD_NEK2_1	399	404	PF00069	0.421
MOD_NEK2_1	476	481	PF00069	0.355
MOD_NEK2_1	54	59	PF00069	0.432
MOD_NEK2_1	589	594	PF00069	0.517
MOD_NEK2_1	612	617	PF00069	0.492
MOD_NEK2_1	642	647	PF00069	0.327
MOD_NEK2_1	744	749	PF00069	0.519
MOD_NEK2_1	791	796	PF00069	0.803
MOD_NEK2_2	208	213	PF00069	0.350
MOD_PIKK_1	401	407	PF00454	0.431
MOD_PIKK_1	481	487	PF00454	0.474
MOD_PIKK_1	495	501	PF00454	0.697
MOD_PIKK_1	57	63	PF00454	0.481
MOD_PKA_2	17	23	PF00069	0.599
MOD_PKA_2	214	220	PF00069	0.350
MOD_PKA_2	276	282	PF00069	0.431
MOD_PKA_2	320	326	PF00069	0.429
MOD_PKA_2	408	414	PF00069	0.431
MOD_PKA_2	477	483	PF00069	0.481
MOD_PKA_2	539	545	PF00069	0.717
MOD_PKA_2	582	588	PF00069	0.662
MOD_PKA_2	589	595	PF00069	0.649
MOD_PKA_2	636	642	PF00069	0.397
MOD_PKA_2	704	710	PF00069	0.497
MOD_Plk_1	282	288	PF00069	0.432
MOD_Plk_1	440	446	PF00069	0.481
MOD_Plk_1	461	467	PF00069	0.481
MOD_Plk_2-3	66	72	PF00069	0.350
MOD_Plk_4	121	127	PF00069	0.409
MOD_Plk_4	215	221	PF00069	0.350
MOD_Plk_4	282	288	PF00069	0.392
MOD_Plk_4	421	427	PF00069	0.468
MOD_Plk_4	450	456	PF00069	0.429
MOD_Plk_4	668	674	PF00069	0.406
MOD_Plk_4	738	744	PF00069	0.677
MOD_ProDKin_1	11	17	PF00069	0.780
MOD_ProDKin_1	118	124	PF00069	0.484
MOD_ProDKin_1	19	25	PF00069	0.694
MOD_ProDKin_1	511	517	PF00069	0.704
MOD_ProDKin_1	533	539	PF00069	0.699
MOD_ProDKin_1	540	546	PF00069	0.692
MOD_ProDKin_1	560	566	PF00069	0.468
MOD_ProDKin_1	735	741	PF00069	0.687
MOD_ProDKin_1	797	803	PF00069	0.586
MOD_ProDKin_1	815	821	PF00069	0.631
MOD_SUMO_for_1	659	662	PF00179	0.332
MOD_SUMO_rev_2	236	246	PF00179	0.481
MOD_SUMO_rev_2	722	732	PF00179	0.431
TRG_DiLeu_BaEn_1	331	336	PF01217	0.431
TRG_DiLeu_BaEn_1	472	477	PF01217	0.432
TRG_DiLeu_BaEn_1	831	836	PF01217	0.669
TRG_DiLeu_BaEn_4	342	348	PF01217	0.432
TRG_DiLeu_BaLyEn_6	168	173	PF01217	0.384
TRG_DiLeu_LyEn_5	331	336	PF01217	0.431
TRG_ENDOCYTIC_2	142	145	PF00928	0.394
TRG_ENDOCYTIC_2	230	233	PF00928	0.339
TRG_ENDOCYTIC_2	679	682	PF00928	0.350
TRG_ER_diArg_1	116	119	PF00400	0.425
TRG_ER_diArg_1	294	297	PF00400	0.481
TRG_ER_diArg_1	515	518	PF00400	0.534
TRG_ER_diArg_1	528	531	PF00400	0.540
TRG_LysEnd_GGAAcLL_1	832	837	PF00790	0.715
TRG_Pf-PMV_PEXEL_1	227	231	PF00026	0.350

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PEH2	Leptomonas seymouri	50%	81%
A0A3R7MNQ1	Trypanosoma rangeli	39%	99%
A0A3S7WQ47	Leishmania donovani	88%	76%
A4HTH3	Leishmania infantum	89%	76%
C9ZPL4	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	38%	100%
E9AMA1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	88%	79%