EF-hand domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Repeat of unknown function (DUF1126), putative

Species:

Leishmania major

UniProt:

Q4QI57_LEIMA

TriTrypDb:

LmjF.08.1120 , LMJLV39_080015200 , LMJSD75_080015300

Length:

869

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 1, no: 28
NetGPI	no	yes: 0, no: 29

Cellular components
Term	Name	Level	Count
GO:0005819	spindle	5	4
GO:0005930	axoneme	2	4
GO:0043226	organelle	2	4
GO:0043228	non-membrane-bounded organelle	3	4
GO:0043229	intracellular organelle	3	4
GO:0043232	intracellular non-membrane-bounded organelle	4	4
GO:0072686	mitotic spindle	6	4
GO:0110165	cellular anatomical entity	1	4

Expansion

Sequence features

Q4QI57

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QI57

Function

Biological processes
Term	Name	Level	Count
GO:0000226	microtubule cytoskeleton organization	3	4
GO:0000281	mitotic cytokinesis	4	4
GO:0000910	cytokinesis	3	4
GO:0001539	cilium or flagellum-dependent cell motility	3	4
GO:0006996	organelle organization	4	4
GO:0007010	cytoskeleton organization	5	4
GO:0007017	microtubule-based process	2	4
GO:0007051	spindle organization	3	4
GO:0007052	mitotic spindle organization	4	4
GO:0009987	cellular process	1	4
GO:0016043	cellular component organization	3	4
GO:0022402	cell cycle process	2	4
GO:0048870	cell motility	2	4
GO:0060285	cilium-dependent cell motility	4	4
GO:0061640	cytoskeleton-dependent cytokinesis	4	4
GO:0071840	cellular component organization or biogenesis	2	4
GO:1902850	microtubule cytoskeleton organization involved in mitosis	4	4
GO:1903047	mitotic cell cycle process	3	4

Molecular functions
Term	Name	Level	Count
GO:0005488	binding	1	20
GO:0005509	calcium ion binding	5	19
GO:0005515	protein binding	2	4
GO:0008092	cytoskeletal protein binding	3	4
GO:0015631	tubulin binding	4	4
GO:0043014	alpha-tubulin binding	5	4
GO:0043167	ion binding	2	19
GO:0043169	cation binding	3	19
GO:0046872	metal ion binding	4	19

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	163	167	PF00656	0.519
CLV_C14_Caspase3-7	290	294	PF00656	0.414
CLV_C14_Caspase3-7	450	454	PF00656	0.740
CLV_C14_Caspase3-7	599	603	PF00656	0.474
CLV_C14_Caspase3-7	741	745	PF00656	0.550
CLV_MEL_PAP_1	30	36	PF00089	0.576
CLV_NRD_NRD_1	100	102	PF00675	0.578
CLV_NRD_NRD_1	139	141	PF00675	0.268
CLV_NRD_NRD_1	301	303	PF00675	0.263
CLV_NRD_NRD_1	423	425	PF00675	0.714
CLV_NRD_NRD_1	497	499	PF00675	0.255
CLV_NRD_NRD_1	530	532	PF00675	0.295
CLV_NRD_NRD_1	728	730	PF00675	0.520
CLV_NRD_NRD_1	767	769	PF00675	0.514
CLV_NRD_NRD_1	789	791	PF00675	0.385
CLV_NRD_NRD_1	864	866	PF00675	0.682
CLV_PCSK_FUR_1	765	769	PF00082	0.588
CLV_PCSK_KEX2_1	172	174	PF00082	0.274
CLV_PCSK_KEX2_1	301	303	PF00082	0.303
CLV_PCSK_KEX2_1	423	425	PF00082	0.692
CLV_PCSK_KEX2_1	497	499	PF00082	0.255
CLV_PCSK_KEX2_1	529	531	PF00082	0.296
CLV_PCSK_KEX2_1	728	730	PF00082	0.539
CLV_PCSK_KEX2_1	767	769	PF00082	0.517
CLV_PCSK_KEX2_1	789	791	PF00082	0.421
CLV_PCSK_KEX2_1	840	842	PF00082	0.454
CLV_PCSK_KEX2_1	864	866	PF00082	0.751
CLV_PCSK_PC1ET2_1	172	174	PF00082	0.268
CLV_PCSK_PC1ET2_1	529	531	PF00082	0.301
CLV_PCSK_PC1ET2_1	840	842	PF00082	0.337
CLV_PCSK_SKI1_1	110	114	PF00082	0.381
CLV_PCSK_SKI1_1	175	179	PF00082	0.274
CLV_PCSK_SKI1_1	220	224	PF00082	0.403
CLV_PCSK_SKI1_1	385	389	PF00082	0.257
CLV_PCSK_SKI1_1	397	401	PF00082	0.351
CLV_PCSK_SKI1_1	424	428	PF00082	0.678
CLV_PCSK_SKI1_1	434	438	PF00082	0.645
CLV_PCSK_SKI1_1	447	451	PF00082	0.735
CLV_PCSK_SKI1_1	483	487	PF00082	0.308
CLV_PCSK_SKI1_1	497	501	PF00082	0.287
CLV_PCSK_SKI1_1	532	536	PF00082	0.326
CLV_PCSK_SKI1_1	728	732	PF00082	0.490
CLV_PCSK_SKI1_1	737	741	PF00082	0.424
DEG_APCC_DBOX_1	219	227	PF00400	0.384
DEG_SPOP_SBC_1	858	862	PF00917	0.508
DEG_SPOP_SBC_1	88	92	PF00917	0.397
DOC_ANK_TNKS_1	291	298	PF00023	0.527
DOC_CKS1_1	782	787	PF01111	0.416
DOC_CYCLIN_RxL_1	431	440	PF00134	0.497
DOC_CYCLIN_yCln2_LP_2	29	32	PF00134	0.563
DOC_CYCLIN_yCln2_LP_2	408	414	PF00134	0.540
DOC_CYCLIN_yCln2_LP_2	486	492	PF00134	0.570
DOC_MAPK_DCC_7	779	788	PF00069	0.394
DOC_MAPK_FxFP_2	811	814	PF00069	0.371
DOC_MAPK_gen_1	140	146	PF00069	0.488
DOC_MAPK_gen_1	301	309	PF00069	0.448
DOC_MAPK_gen_1	529	536	PF00069	0.414
DOC_MAPK_gen_1	644	652	PF00069	0.400
DOC_MAPK_MEF2A_6	301	309	PF00069	0.513
DOC_MAPK_MEF2A_6	370	378	PF00069	0.490
DOC_PP1_RVXF_1	115	122	PF00149	0.411
DOC_PP1_RVXF_1	279	286	PF00149	0.445
DOC_PP2B_LxvP_1	29	32	PF13499	0.555
DOC_PP2B_LxvP_1	408	411	PF13499	0.535
DOC_PP4_FxxP_1	125	128	PF00568	0.539
DOC_PP4_FxxP_1	71	74	PF00568	0.730
DOC_PP4_FxxP_1	774	777	PF00568	0.641
DOC_PP4_FxxP_1	811	814	PF00568	0.348
DOC_SPAK_OSR1_1	498	502	PF12202	0.486
DOC_USP7_MATH_1	12	16	PF00917	0.705
DOC_USP7_MATH_1	432	436	PF00917	0.735
DOC_USP7_MATH_1	462	466	PF00917	0.497
DOC_USP7_MATH_1	730	734	PF00917	0.580
DOC_USP7_MATH_1	858	862	PF00917	0.736
DOC_USP7_MATH_1	88	92	PF00917	0.588
DOC_USP7_UBL2_3	535	539	PF12436	0.498
DOC_WW_Pin1_4	319	324	PF00397	0.469
DOC_WW_Pin1_4	781	786	PF00397	0.384
DOC_WW_Pin1_4	796	801	PF00397	0.428
DOC_WW_Pin1_4	90	95	PF00397	0.425
LIG_14-3-3_CanoR_1	110	115	PF00244	0.304
LIG_14-3-3_CanoR_1	204	213	PF00244	0.443
LIG_14-3-3_CanoR_1	220	230	PF00244	0.264
LIG_14-3-3_CanoR_1	319	323	PF00244	0.513
LIG_14-3-3_CanoR_1	403	409	PF00244	0.554
LIG_14-3-3_CanoR_1	423	427	PF00244	0.566
LIG_14-3-3_CanoR_1	497	502	PF00244	0.465
LIG_14-3-3_CanoR_1	67	72	PF00244	0.694
LIG_14-3-3_CanoR_1	710	715	PF00244	0.687
LIG_14-3-3_CanoR_1	755	761	PF00244	0.400
LIG_14-3-3_CanoR_1	8	12	PF00244	0.701
LIG_APCC_ABBA_1	305	310	PF00400	0.510
LIG_BIR_II_1	1	5	PF00653	0.655
LIG_BIR_III_4	456	460	PF00653	0.529
LIG_BIR_III_4	609	613	PF00653	0.584
LIG_BIR_III_4	697	701	PF00653	0.413
LIG_BRCT_BRCA1_1	192	196	PF00533	0.565
LIG_BRCT_BRCA1_1	253	257	PF00533	0.572
LIG_BRCT_BRCA1_1	271	275	PF00533	0.552
LIG_BRCT_BRCA1_1	414	418	PF00533	0.546
LIG_BRCT_BRCA1_1	506	510	PF00533	0.442
LIG_FHA_1	150	156	PF00498	0.453
LIG_FHA_1	377	383	PF00498	0.461
LIG_FHA_1	431	437	PF00498	0.634
LIG_FHA_1	828	834	PF00498	0.544
LIG_FHA_2	111	117	PF00498	0.490
LIG_FHA_2	448	454	PF00498	0.641
LIG_FHA_2	557	563	PF00498	0.468
LIG_FHA_2	593	599	PF00498	0.598
LIG_FHA_2	686	692	PF00498	0.376
LIG_FHA_2	773	779	PF00498	0.580
LIG_FHA_2	799	805	PF00498	0.399
LIG_GBD_Chelix_1	620	628	PF00786	0.459
LIG_LIR_Apic_2	69	74	PF02991	0.508
LIG_LIR_Apic_2	773	777	PF02991	0.559
LIG_LIR_Apic_2	810	814	PF02991	0.439
LIG_LIR_Gen_1	189	199	PF02991	0.447
LIG_LIR_Gen_1	638	645	PF02991	0.493
LIG_LIR_Gen_1	684	693	PF02991	0.451
LIG_LIR_Nem_3	145	149	PF02991	0.557
LIG_LIR_Nem_3	189	194	PF02991	0.427
LIG_LIR_Nem_3	208	213	PF02991	0.469
LIG_LIR_Nem_3	243	248	PF02991	0.457
LIG_LIR_Nem_3	254	260	PF02991	0.485
LIG_LIR_Nem_3	366	372	PF02991	0.545
LIG_LIR_Nem_3	479	485	PF02991	0.561
LIG_LIR_Nem_3	507	513	PF02991	0.482
LIG_LIR_Nem_3	638	642	PF02991	0.387
LIG_LIR_Nem_3	684	690	PF02991	0.478
LIG_LIR_Nem_3	810	816	PF02991	0.472
LIG_MLH1_MIPbox_1	414	418	PF16413	0.546
LIG_MYND_1	27	31	PF01753	0.656
LIG_Pex14_2	121	125	PF04695	0.462
LIG_Pex14_2	218	222	PF04695	0.481
LIG_REV1ctd_RIR_1	397	407	PF16727	0.346
LIG_SH2_GRB2like	504	507	PF00017	0.592
LIG_SH2_NCK_1	513	517	PF00017	0.533
LIG_SH2_PTP2	191	194	PF00017	0.545
LIG_SH2_SRC	627	630	PF00017	0.512
LIG_SH2_STAP1	395	399	PF00017	0.516
LIG_SH2_STAP1	513	517	PF00017	0.494
LIG_SH2_STAP1	589	593	PF00017	0.376
LIG_SH2_STAP1	831	835	PF00017	0.483
LIG_SH2_STAT5	191	194	PF00017	0.502
LIG_SH2_STAT5	21	24	PF00017	0.613
LIG_SH2_STAT5	330	333	PF00017	0.510
LIG_SH2_STAT5	395	398	PF00017	0.531
LIG_SH2_STAT5	417	420	PF00017	0.597
LIG_SH2_STAT5	504	507	PF00017	0.452
LIG_SH2_STAT5	558	561	PF00017	0.463
LIG_SH2_STAT5	627	630	PF00017	0.458
LIG_SH2_STAT5	686	689	PF00017	0.400
LIG_SH2_STAT5	807	810	PF00017	0.440
LIG_SH2_STAT5	821	824	PF00017	0.506
LIG_SH2_STAT5	843	846	PF00017	0.343
LIG_SH3_1	543	549	PF00018	0.524
LIG_SH3_3	174	180	PF00018	0.529
LIG_SH3_3	227	233	PF00018	0.380
LIG_SH3_3	442	448	PF00018	0.680
LIG_SH3_3	534	540	PF00018	0.552
LIG_SH3_3	543	549	PF00018	0.496
LIG_SH3_3	647	653	PF00018	0.559
LIG_SH3_3	779	785	PF00018	0.534
LIG_SH3_3	811	817	PF00018	0.396
LIG_SH3_CIN85_PxpxPR_1	538	543	PF14604	0.545
LIG_SUMO_SIM_anti_2	688	694	PF11976	0.539
LIG_SUMO_SIM_par_1	150	157	PF11976	0.456
LIG_SUMO_SIM_par_1	410	416	PF11976	0.557
LIG_SUMO_SIM_par_1	613	618	PF11976	0.515
LIG_SUMO_SIM_par_1	647	654	PF11976	0.419
LIG_TRAF2_1	187	190	PF00917	0.486
LIG_WRC_WIRS_1	143	148	PF05994	0.423
LIG_WRC_WIRS_1	636	641	PF05994	0.443
LIG_WRC_WIRS_1	771	776	PF05994	0.435
LIG_WW_3	30	34	PF00397	0.578
MOD_CDC14_SPxK_1	93	96	PF00782	0.430
MOD_CDK_SPxK_1	90	96	PF00069	0.426
MOD_CK1_1	322	328	PF00069	0.407
MOD_CK1_1	440	446	PF00069	0.630
MOD_CK1_1	713	719	PF00069	0.614
MOD_CK2_1	262	268	PF00069	0.455
MOD_CK2_1	318	324	PF00069	0.331
MOD_CK2_1	592	598	PF00069	0.565
MOD_CK2_1	648	654	PF00069	0.406
MOD_CK2_1	798	804	PF00069	0.443
MOD_Cter_Amidation	765	768	PF01082	0.540
MOD_GlcNHglycan	162	165	PF01048	0.301
MOD_GlcNHglycan	253	256	PF01048	0.568
MOD_GlcNHglycan	263	267	PF01048	0.548
MOD_GlcNHglycan	338	341	PF01048	0.379
MOD_GlcNHglycan	514	517	PF01048	0.317
MOD_GlcNHglycan	715	718	PF01048	0.632
MOD_GSK3_1	258	265	PF00069	0.567
MOD_GSK3_1	318	325	PF00069	0.335
MOD_GSK3_1	422	429	PF00069	0.604
MOD_GSK3_1	619	626	PF00069	0.485
MOD_GSK3_1	627	634	PF00069	0.421
MOD_GSK3_1	63	70	PF00069	0.689
MOD_GSK3_1	681	688	PF00069	0.519
MOD_GSK3_1	794	801	PF00069	0.521
MOD_GSK3_1	860	867	PF00069	0.779
MOD_LATS_1	629	635	PF00433	0.545
MOD_N-GLC_1	12	17	PF02516	0.609
MOD_N-GLC_1	205	210	PF02516	0.239
MOD_N-GLC_1	67	72	PF02516	0.621
MOD_N-GLC_1	826	831	PF02516	0.527
MOD_NEK2_1	329	334	PF00069	0.316
MOD_NEK2_1	383	388	PF00069	0.449
MOD_NEK2_1	437	442	PF00069	0.566
MOD_NEK2_1	499	504	PF00069	0.274
MOD_NEK2_1	556	561	PF00069	0.200
MOD_NEK2_1	57	62	PF00069	0.688
MOD_NEK2_1	7	12	PF00069	0.654
MOD_NEK2_1	756	761	PF00069	0.385
MOD_NEK2_1	78	83	PF00069	0.595
MOD_NEK2_1	89	94	PF00069	0.547
MOD_NEK2_2	404	409	PF00069	0.357
MOD_NEK2_2	476	481	PF00069	0.423
MOD_PIKK_1	258	264	PF00454	0.517
MOD_PIKK_1	504	510	PF00454	0.487
MOD_PIKK_1	819	825	PF00454	0.589
MOD_PK_1	338	344	PF00069	0.374
MOD_PKA_1	497	503	PF00069	0.340
MOD_PKA_2	291	297	PF00069	0.408
MOD_PKA_2	318	324	PF00069	0.326
MOD_PKA_2	422	428	PF00069	0.625
MOD_PKA_2	497	503	PF00069	0.368
MOD_PKA_2	57	63	PF00069	0.693
MOD_PKA_2	66	72	PF00069	0.723
MOD_PKA_2	681	687	PF00069	0.573
MOD_PKA_2	7	13	PF00069	0.711
MOD_PKA_2	778	784	PF00069	0.499
MOD_Plk_1	149	155	PF00069	0.304
MOD_Plk_1	269	275	PF00069	0.513
MOD_Plk_1	310	316	PF00069	0.297
MOD_Plk_1	346	352	PF00069	0.361
MOD_Plk_1	412	418	PF00069	0.464
MOD_Plk_1	653	659	PF00069	0.530
MOD_Plk_1	67	73	PF00069	0.660
MOD_Plk_4	133	139	PF00069	0.386
MOD_Plk_4	149	155	PF00069	0.151
MOD_Plk_4	413	419	PF00069	0.586
MOD_Plk_4	432	438	PF00069	0.672
MOD_Plk_4	499	505	PF00069	0.268
MOD_Plk_4	584	590	PF00069	0.479
MOD_Plk_4	635	641	PF00069	0.531
MOD_Plk_4	778	784	PF00069	0.481
MOD_Plk_4	846	852	PF00069	0.611
MOD_ProDKin_1	319	325	PF00069	0.316
MOD_ProDKin_1	781	787	PF00069	0.381
MOD_ProDKin_1	796	802	PF00069	0.428
MOD_ProDKin_1	90	96	PF00069	0.426
MOD_SUMO_rev_2	561	569	PF00179	0.420
MOD_SUMO_rev_2	716	726	PF00179	0.561
MOD_SUMO_rev_2	733	741	PF00179	0.495
TRG_DiLeu_BaEn_1	804	809	PF01217	0.309
TRG_DiLeu_BaEn_2	303	309	PF01217	0.314
TRG_DiLeu_BaEn_4	189	195	PF01217	0.423
TRG_ENDOCYTIC_2	122	125	PF00928	0.330
TRG_ENDOCYTIC_2	191	194	PF00928	0.417
TRG_ENDOCYTIC_2	286	289	PF00928	0.369
TRG_ENDOCYTIC_2	369	372	PF00928	0.423
TRG_ENDOCYTIC_2	842	845	PF00928	0.442
TRG_ER_diArg_1	180	183	PF00400	0.306
TRG_ER_diArg_1	300	302	PF00400	0.418
TRG_ER_diArg_1	497	499	PF00400	0.303
TRG_ER_diArg_1	764	767	PF00400	0.542
TRG_ER_diArg_1	788	790	PF00400	0.362
TRG_NLS_MonoCore_2	528	533	PF00514	0.423
TRG_Pf-PMV_PEXEL_1	274	278	PF00026	0.457
TRG_Pf-PMV_PEXEL_1	734	738	PF00026	0.443
TRG_Pf-PMV_PEXEL_1	790	794	PF00026	0.362

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P5H4	Leptomonas seymouri	70%	100%
A0A0N1HZ98	Leptomonas seymouri	22%	100%
A0A0N1I3M2	Leptomonas seymouri	22%	100%
A0A0N1PBW1	Leptomonas seymouri	25%	100%
A0A0S4IJW8	Bodo saltans	35%	100%
A0A1X0NEV8	Trypanosomatidae	46%	100%
A0A1X0NLI2	Trypanosomatidae	24%	100%
A0A3Q8IDH4	Leishmania donovani	23%	100%
A0A3Q8IPU3	Leishmania donovani	24%	100%
A0A3R7KM50	Trypanosoma rangeli	47%	100%
A0A3R7L048	Trypanosoma rangeli	24%	100%
A0A3S5H688	Leishmania donovani	96%	100%
A0A422NK00	Trypanosoma rangeli	27%	100%
A4HE78	Leishmania braziliensis	23%	100%
A4HFK9	Leishmania braziliensis	23%	100%
A4HTP5	Leishmania infantum	95%	100%
A4I1J2	Leishmania infantum	23%	100%
C9ZJX4	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	22%	100%
C9ZPE7	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	44%	100%
D0A5T5	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	23%	100%
E9AD64	Leishmania major	23%	100%
E9AHF6	Leishmania infantum	23%	100%
E9AI34	Leishmania braziliensis	87%	100%
E9AMI2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	94%	100%
E9AXM7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	24%	100%
E9AYY9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	22%	100%
Q4Q9U5	Leishmania major	24%	100%
V5ASV2	Trypanosoma cruzi	24%	100%