MIX domain-containing protein

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

MIX protein

Species:

Leishmania major

UniProt:

Q4QI49_LEIMA

TriTrypDb:

LmjF.08.1200 , LMJLV39_080016000 , LMJSD75_080016100

Length:

195

Annotations

LeishMANIAdb annotations

Appears to be a small signal-anchored protein, specific to Kinetoplastids. Generally not duplicated, only in Strigomonas

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	4
GO:0110165	cellular anatomical entity	1	4

Expansion

Sequence features

Q4QI49

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QI49

PDB structure(s): 3ha4_A , 3ha4_B , 3ha4_C , 3ha4_D , 3ha4_E , 3ha4_F , 3ha4_G , 3ha4_H

Function

Could not find GO biological_process term for this entry.

Molecular functions
Term	Name	Level	Count
GO:0005488	binding	1	2
GO:0005515	protein binding	2	2
GO:0042802	identical protein binding	3	2
GO:0042803	protein homodimerization activity	4	2
GO:0046983	protein dimerization activity	3	2

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	138	140	PF00675	0.296
CLV_NRD_NRD_1	7	9	PF00675	0.685
CLV_NRD_NRD_1	71	73	PF00675	0.297
CLV_PCSK_KEX2_1	7	9	PF00082	0.685
CLV_PCSK_KEX2_1	71	73	PF00082	0.300
CLV_PCSK_PC7_1	3	9	PF00082	0.671
CLV_PCSK_SKI1_1	140	144	PF00082	0.296
CLV_PCSK_SKI1_1	26	30	PF00082	0.496
CLV_PCSK_SKI1_1	81	85	PF00082	0.282
DEG_Nend_Nbox_1	1	3	PF02207	0.491
DOC_MAPK_gen_1	139	146	PF00069	0.496
DOC_MAPK_gen_1	46	56	PF00069	0.516
DOC_USP7_UBL2_3	46	50	PF12436	0.496
LIG_14-3-3_CanoR_1	153	157	PF00244	0.553
LIG_14-3-3_CanoR_1	3	9	PF00244	0.487
LIG_BRCT_BRCA1_1	35	39	PF00533	0.346
LIG_Clathr_ClatBox_1	111	115	PF01394	0.482
LIG_CtBP_PxDLS_1	113	117	PF00389	0.534
LIG_eIF4E_1	106	112	PF01652	0.482
LIG_FHA_1	115	121	PF00498	0.496
LIG_FHA_2	14	20	PF00498	0.510
LIG_LIR_Apic_2	109	113	PF02991	0.487
LIG_LIR_Gen_1	36	45	PF02991	0.337
LIG_LIR_Nem_3	13	18	PF02991	0.504
LIG_LIR_Nem_3	36	42	PF02991	0.337
LIG_NRBOX	142	148	PF00104	0.496
LIG_Pex14_2	29	33	PF04695	0.335
LIG_PTB_Apo_2	145	152	PF02174	0.496
LIG_PTB_Phospho_1	145	151	PF10480	0.496
LIG_SH2_CRK	151	155	PF00017	0.492
LIG_SH2_STAT5	127	130	PF00017	0.492
LIG_SH2_STAT5	131	134	PF00017	0.496
LIG_SH2_STAT5	23	26	PF00017	0.384
LIG_SH3_3	129	135	PF00018	0.482
LIG_SH3_3	151	157	PF00018	0.465
LIG_SH3_3	186	192	PF00018	0.534
LIG_SH3_3	67	73	PF00018	0.482
LIG_SUMO_SIM_anti_2	93	100	PF11976	0.534
LIG_SUMO_SIM_par_1	112	117	PF11976	0.512
LIG_SUMO_SIM_par_1	52	59	PF11976	0.534
LIG_SUMO_SIM_par_1	81	87	PF11976	0.525
LIG_TYR_ITIM	149	154	PF00017	0.492
LIG_UBA3_1	143	150	PF00899	0.497
LIG_WRC_WIRS_1	12	17	PF05994	0.519
MOD_CK1_1	10	16	PF00069	0.517
MOD_CK1_1	41	47	PF00069	0.355
MOD_CK2_1	13	19	PF00069	0.514
MOD_CK2_1	41	47	PF00069	0.336
MOD_GlcNHglycan	123	126	PF01048	0.296
MOD_GSK3_1	41	48	PF00069	0.357
MOD_GSK3_1	7	14	PF00069	0.520
MOD_NEK2_1	114	119	PF00069	0.482
MOD_NEK2_1	120	125	PF00069	0.482
MOD_NEK2_1	2	7	PF00069	0.465
MOD_NEK2_1	33	38	PF00069	0.347
MOD_NEK2_2	38	43	PF00069	0.355
MOD_PIKK_1	114	120	PF00454	0.482
MOD_PKA_1	7	13	PF00069	0.503
MOD_PKA_2	152	158	PF00069	0.562
MOD_PKA_2	2	8	PF00069	0.474
MOD_Plk_1	114	120	PF00069	0.482
MOD_Plk_4	127	133	PF00069	0.544
MOD_Plk_4	24	30	PF00069	0.298
MOD_Plk_4	38	44	PF00069	0.256
MOD_SUMO_rev_2	41	51	PF00179	0.355
TRG_DiLeu_BaEn_1	93	98	PF01217	0.562
TRG_DiLeu_BaLyEn_6	73	78	PF01217	0.496
TRG_ENDOCYTIC_2	151	154	PF00928	0.492
TRG_ER_diArg_1	7	9	PF00400	0.489
TRG_ER_diArg_1	70	72	PF00400	0.493

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I3I0	Leptomonas seymouri	92%	100%
A0A0S4IQY8	Bodo saltans	55%	100%
A0A1X0NFN6	Trypanosomatidae	74%	98%
A0A3R7KDG8	Trypanosoma rangeli	74%	98%
A0A3S5H693	Leishmania donovani	97%	100%
A4H5F8	Leishmania braziliensis	94%	100%
A4HTQ3	Leishmania infantum	98%	100%
C9ZPF9	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	68%	98%
E9AMJ0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	99%	100%