E3 ubiquitin-protein ligase CHFR

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

FHA domain/Ring finger domain containing protein, putative

Species:

Leishmania major

UniProt:

Q4QI21_LEIMA

TriTrypDb:

LmjF.09.0140 , LMJLV39_090006600 , LMJSD75_090006600

Length:

524

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	2
GO:0016604	nuclear body	2	12
GO:0016605	PML body	3	12
GO:0031974	membrane-enclosed lumen	2	2
GO:0031981	nuclear lumen	5	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0043233	organelle lumen	3	2
GO:0070013	intracellular organelle lumen	4	2
GO:0110165	cellular anatomical entity	1	12
GO:0016020	membrane	2	1

Expansion

Sequence features

Q4QI21

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QI21

Function

Biological processes
Term	Name	Level	Count
GO:0006508	proteolysis	4	2
GO:0006511	ubiquitin-dependent protein catabolic process	7	2
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008152	metabolic process	1	12
GO:0009056	catabolic process	2	2
GO:0009057	macromolecule catabolic process	4	2
GO:0009987	cellular process	1	2
GO:0016567	protein ubiquitination	7	12
GO:0019538	protein metabolic process	3	12
GO:0019941	modification-dependent protein catabolic process	6	2
GO:0032446	protein modification by small protein conjugation	6	12
GO:0036211	protein modification process	4	12
GO:0043170	macromolecule metabolic process	3	12
GO:0043412	macromolecule modification	4	12
GO:0043632	modification-dependent macromolecule catabolic process	5	2
GO:0044237	cellular metabolic process	2	2
GO:0044238	primary metabolic process	2	12
GO:0044248	cellular catabolic process	3	2
GO:0044260	obsolete cellular macromolecule metabolic process	3	2
GO:0044265	obsolete cellular macromolecule catabolic process	4	2
GO:0051603	proteolysis involved in protein catabolic process	5	2
GO:0070647	protein modification by small protein conjugation or removal	5	12
GO:0071704	organic substance metabolic process	2	12
GO:1901564	organonitrogen compound metabolic process	3	12
GO:1901575	organic substance catabolic process	3	2

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	2
GO:0004842	ubiquitin-protein transferase activity	4	2
GO:0005488	binding	1	12
GO:0016740	transferase activity	2	2
GO:0019787	ubiquitin-like protein transferase activity	3	2
GO:0043167	ion binding	2	12
GO:0043169	cation binding	3	12
GO:0046872	metal ion binding	4	12
GO:0140096	catalytic activity, acting on a protein	2	2

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	292	296	PF00656	0.515
CLV_C14_Caspase3-7	329	333	PF00656	0.262
CLV_NRD_NRD_1	116	118	PF00675	0.286
CLV_NRD_NRD_1	251	253	PF00675	0.444
CLV_NRD_NRD_1	461	463	PF00675	0.356
CLV_NRD_NRD_1	476	478	PF00675	0.193
CLV_NRD_NRD_1	521	523	PF00675	0.489
CLV_PCSK_KEX2_1	116	118	PF00082	0.407
CLV_PCSK_KEX2_1	251	253	PF00082	0.473
CLV_PCSK_KEX2_1	275	277	PF00082	0.520
CLV_PCSK_KEX2_1	461	463	PF00082	0.356
CLV_PCSK_KEX2_1	476	478	PF00082	0.190
CLV_PCSK_KEX2_1	490	492	PF00082	0.216
CLV_PCSK_KEX2_1	521	523	PF00082	0.481
CLV_PCSK_PC1ET2_1	275	277	PF00082	0.499
CLV_PCSK_PC1ET2_1	490	492	PF00082	0.311
CLV_PCSK_PC1ET2_1	521	523	PF00082	0.481
CLV_PCSK_SKI1_1	117	121	PF00082	0.350
CLV_PCSK_SKI1_1	123	127	PF00082	0.329
CLV_PCSK_SKI1_1	131	135	PF00082	0.267
CLV_PCSK_SKI1_1	187	191	PF00082	0.301
DEG_APCC_DBOX_1	99	107	PF00400	0.385
DEG_SCF_TRCP1_1	295	300	PF00400	0.468
DOC_CDC14_PxL_1	50	58	PF14671	0.492
DOC_CYCLIN_yCln2_LP_2	38	44	PF00134	0.531
DOC_MAPK_gen_1	116	126	PF00069	0.243
DOC_MAPK_gen_1	302	311	PF00069	0.529
DOC_MAPK_RevD_3	101	117	PF00069	0.377
DOC_PP2B_LxvP_1	33	36	PF13499	0.569
DOC_PP4_FxxP_1	190	193	PF00568	0.334
DOC_PP4_FxxP_1	334	337	PF00568	0.300
DOC_USP7_MATH_1	137	141	PF00917	0.581
DOC_USP7_MATH_1	347	351	PF00917	0.380
DOC_USP7_MATH_1	420	424	PF00917	0.263
DOC_USP7_MATH_1	7	11	PF00917	0.663
DOC_WW_Pin1_4	11	16	PF00397	0.661
DOC_WW_Pin1_4	244	249	PF00397	0.454
DOC_WW_Pin1_4	3	8	PF00397	0.726
DOC_WW_Pin1_4	324	329	PF00397	0.385
DOC_WW_Pin1_4	454	459	PF00397	0.322
LIG_14-3-3_CanoR_1	192	197	PF00244	0.274
LIG_14-3-3_CanoR_1	224	232	PF00244	0.353
LIG_14-3-3_CanoR_1	235	244	PF00244	0.552
LIG_14-3-3_CanoR_1	344	352	PF00244	0.385
LIG_14-3-3_CanoR_1	462	468	PF00244	0.437
LIG_14-3-3_CanoR_1	477	485	PF00244	0.397
LIG_Actin_WH2_2	412	429	PF00022	0.173
LIG_APCC_ABBA_1	388	393	PF00400	0.308
LIG_BIR_II_1	1	5	PF00653	0.620
LIG_BIR_III_4	141	145	PF00653	0.488
LIG_EVH1_2	401	405	PF00568	0.334
LIG_FHA_1	180	186	PF00498	0.261
LIG_FHA_1	231	237	PF00498	0.372
LIG_FHA_1	53	59	PF00498	0.462
LIG_FHA_1	96	102	PF00498	0.283
LIG_Integrin_isoDGR_2	114	116	PF01839	0.286
LIG_Integrin_isoDGR_2	158	160	PF01839	0.524
LIG_LIR_Apic_2	188	193	PF02991	0.388
LIG_LIR_Apic_2	332	337	PF02991	0.255
LIG_LIR_Gen_1	162	171	PF02991	0.378
LIG_LIR_Gen_1	395	405	PF02991	0.263
LIG_LIR_Gen_1	466	475	PF02991	0.248
LIG_LIR_Nem_3	162	168	PF02991	0.351
LIG_LIR_Nem_3	386	391	PF02991	0.427
LIG_LIR_Nem_3	395	400	PF02991	0.207
LIG_LIR_Nem_3	466	471	PF02991	0.302
LIG_PCNA_PIPBox_1	412	421	PF02747	0.240
LIG_RPA_C_Fungi	472	484	PF08784	0.173
LIG_SH2_CRK	50	54	PF00017	0.516
LIG_SH2_STAT3	163	166	PF00017	0.391
LIG_SH2_STAT5	243	246	PF00017	0.384
LIG_SH2_STAT5	418	421	PF00017	0.286
LIG_SH3_3	396	402	PF00018	0.423
LIG_TRAF2_1	253	256	PF00917	0.468
LIG_UBA3_1	164	169	PF00899	0.448
MOD_CDK_SPK_2	3	8	PF00069	0.591
MOD_CDK_SPxxK_3	11	18	PF00069	0.614
MOD_CDK_SPxxK_3	244	251	PF00069	0.308
MOD_CDK_SPxxK_3	454	461	PF00069	0.263
MOD_CK1_1	247	253	PF00069	0.480
MOD_CK1_1	3	9	PF00069	0.745
MOD_CK1_1	406	412	PF00069	0.314
MOD_CK2_1	235	241	PF00069	0.415
MOD_Cter_Amidation	114	117	PF01082	0.337
MOD_Cter_Amidation	273	276	PF01082	0.605
MOD_DYRK1A_RPxSP_1	454	458	PF00069	0.263
MOD_GlcNHglycan	149	152	PF01048	0.605
MOD_GlcNHglycan	177	180	PF01048	0.542
MOD_GlcNHglycan	208	211	PF01048	0.336
MOD_GlcNHglycan	295	298	PF01048	0.580
MOD_GlcNHglycan	345	348	PF01048	0.308
MOD_GlcNHglycan	405	408	PF01048	0.263
MOD_GlcNHglycan	430	433	PF01048	0.384
MOD_GlcNHglycan	61	64	PF01048	0.509
MOD_GlcNHglycan	85	88	PF01048	0.310
MOD_GSK3_1	104	111	PF00069	0.318
MOD_GSK3_1	175	182	PF00069	0.420
MOD_GSK3_1	274	281	PF00069	0.569
MOD_GSK3_1	289	296	PF00069	0.553
MOD_GSK3_1	3	10	PF00069	0.683
MOD_GSK3_1	343	350	PF00069	0.263
MOD_GSK3_1	379	386	PF00069	0.394
MOD_GSK3_1	79	86	PF00069	0.394
MOD_N-GLC_1	108	113	PF02516	0.240
MOD_N-GLC_1	79	84	PF02516	0.173
MOD_N-GLC_2	282	284	PF02516	0.428
MOD_NEK2_1	181	186	PF00069	0.288
MOD_NEK2_1	343	348	PF00069	0.343
MOD_NEK2_2	297	302	PF00069	0.538
MOD_NEK2_2	347	352	PF00069	0.344
MOD_NEK2_2	463	468	PF00069	0.334
MOD_OFUCOSY	345	351	PF10250	0.263
MOD_PK_1	192	198	PF00069	0.334
MOD_PKA_2	175	181	PF00069	0.426
MOD_PKA_2	223	229	PF00069	0.380
MOD_PKA_2	230	236	PF00069	0.313
MOD_PKA_2	343	349	PF00069	0.363
MOD_PKA_2	7	13	PF00069	0.659
MOD_PKB_1	276	284	PF00069	0.545
MOD_Plk_4	108	114	PF00069	0.240
MOD_Plk_4	185	191	PF00069	0.321
MOD_Plk_4	192	198	PF00069	0.306
MOD_Plk_4	420	426	PF00069	0.332
MOD_Plk_4	463	469	PF00069	0.293
MOD_ProDKin_1	11	17	PF00069	0.659
MOD_ProDKin_1	244	250	PF00069	0.453
MOD_ProDKin_1	3	9	PF00069	0.725
MOD_ProDKin_1	324	330	PF00069	0.385
MOD_ProDKin_1	454	460	PF00069	0.322
MOD_SUMO_for_1	119	122	PF00179	0.377
MOD_SUMO_for_1	168	171	PF00179	0.461
MOD_SUMO_for_1	435	438	PF00179	0.361
TRG_DiLeu_BaEn_4	255	261	PF01217	0.423
TRG_ENDOCYTIC_2	50	53	PF00928	0.513
TRG_ENDOCYTIC_2	513	516	PF00928	0.273
TRG_ER_diArg_1	116	118	PF00400	0.286
TRG_ER_diArg_1	174	177	PF00400	0.552
TRG_ER_diArg_1	251	253	PF00400	0.399
TRG_ER_diArg_1	475	477	PF00400	0.255
TRG_NLS_MonoExtC_3	274	280	PF00514	0.494
TRG_NLS_MonoExtN_4	272	279	PF00514	0.417
TRG_Pf-PMV_PEXEL_1	477	482	PF00026	0.409

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P377	Leptomonas seymouri	62%	98%
A0A0S4JT63	Bodo saltans	40%	100%
A0A1X0NPC5	Trypanosomatidae	44%	99%
A0A3R7MDE3	Trypanosoma rangeli	43%	96%
A0A3S5H6B1	Leishmania donovani	94%	100%
A4H5J1	Leishmania braziliensis	75%	100%
A4HTT2	Leishmania infantum	94%	100%
A5WW08	Danio rerio	30%	82%
D0A993	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	42%	94%
E9AML5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	90%	100%
Q5FWP4	Xenopus laevis	29%	84%
Q5RF77	Pongo abelii	28%	92%
Q6P256	Xenopus tropicalis	29%	84%
Q810L3	Mus musculus	30%	79%
Q96EP1	Homo sapiens	30%	79%
V5DJC2	Trypanosoma cruzi	43%	84%