DNA helicase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

DNA helicase

Gene product:

ATP-dependent DNA helicase, putative

Species:

Leishmania major

UniProt:

Q4QHW5_LEIMA

TriTrypDb:

LmjF.09.0590 , LMJLV39_090011700 , LMJSD75_090011400

Length:

996

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 6
NetGPI	no	yes: 0, no: 6

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	2
GO:0031974	membrane-enclosed lumen	2	2
GO:0031981	nuclear lumen	5	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0043233	organelle lumen	3	2
GO:0070013	intracellular organelle lumen	4	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

Q4QHW5

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QHW5

Function

Biological processes
Term	Name	Level	Count
GO:0000725	recombinational repair	6	2
GO:0006139	nucleobase-containing compound metabolic process	3	2
GO:0006259	DNA metabolic process	4	2
GO:0006281	DNA repair	5	2
GO:0006310	DNA recombination	5	2
GO:0006725	cellular aromatic compound metabolic process	3	2
GO:0006807	nitrogen compound metabolic process	2	2
GO:0006950	response to stress	2	2
GO:0006974	DNA damage response	4	2
GO:0008152	metabolic process	1	2
GO:0009987	cellular process	1	2
GO:0033554	cellular response to stress	3	2
GO:0034641	cellular nitrogen compound metabolic process	3	2
GO:0043170	macromolecule metabolic process	3	2
GO:0044237	cellular metabolic process	2	2
GO:0044238	primary metabolic process	2	2
GO:0044260	obsolete cellular macromolecule metabolic process	3	2
GO:0046483	heterocycle metabolic process	3	2
GO:0050896	response to stimulus	1	2
GO:0051716	cellular response to stimulus	2	2
GO:0071704	organic substance metabolic process	2	2
GO:0090304	nucleic acid metabolic process	4	2
GO:1901360	organic cyclic compound metabolic process	3	2

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	7
GO:0003676	nucleic acid binding	3	7
GO:0003677	DNA binding	4	7
GO:0003678	DNA helicase activity	3	7
GO:0003824	catalytic activity	1	7
GO:0004386	helicase activity	2	7
GO:0005488	binding	1	7
GO:0005524	ATP binding	5	7
GO:0008094	ATP-dependent activity, acting on DNA	2	7
GO:0016787	hydrolase activity	2	7
GO:0017076	purine nucleotide binding	4	7
GO:0030554	adenyl nucleotide binding	5	7
GO:0032553	ribonucleotide binding	3	7
GO:0032555	purine ribonucleotide binding	4	7
GO:0032559	adenyl ribonucleotide binding	5	7
GO:0035639	purine ribonucleoside triphosphate binding	4	7
GO:0036094	small molecule binding	2	7
GO:0043138	3'-5' DNA helicase activity	4	2
GO:0043167	ion binding	2	7
GO:0043168	anion binding	3	7
GO:0097159	organic cyclic compound binding	2	7
GO:0097367	carbohydrate derivative binding	2	7
GO:0140097	catalytic activity, acting on DNA	3	7
GO:0140640	catalytic activity, acting on a nucleic acid	2	7
GO:0140657	ATP-dependent activity	1	7
GO:1901265	nucleoside phosphate binding	3	7
GO:1901363	heterocyclic compound binding	2	7

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	703	707	PF00656	0.729
CLV_NRD_NRD_1	116	118	PF00675	0.433
CLV_NRD_NRD_1	365	367	PF00675	0.411
CLV_NRD_NRD_1	383	385	PF00675	0.411
CLV_NRD_NRD_1	466	468	PF00675	0.633
CLV_NRD_NRD_1	697	699	PF00675	0.777
CLV_NRD_NRD_1	94	96	PF00675	0.400
CLV_PCSK_FUR_1	380	384	PF00082	0.411
CLV_PCSK_KEX2_1	116	118	PF00082	0.433
CLV_PCSK_KEX2_1	297	299	PF00082	0.496
CLV_PCSK_KEX2_1	364	366	PF00082	0.411
CLV_PCSK_KEX2_1	382	384	PF00082	0.411
CLV_PCSK_KEX2_1	466	468	PF00082	0.633
CLV_PCSK_KEX2_1	620	622	PF00082	0.742
CLV_PCSK_KEX2_1	697	699	PF00082	0.783
CLV_PCSK_KEX2_1	914	916	PF00082	0.411
CLV_PCSK_KEX2_1	94	96	PF00082	0.400
CLV_PCSK_PC1ET2_1	297	299	PF00082	0.496
CLV_PCSK_PC1ET2_1	620	622	PF00082	0.742
CLV_PCSK_PC1ET2_1	914	916	PF00082	0.411
CLV_PCSK_PC7_1	910	916	PF00082	0.411
CLV_PCSK_SKI1_1	323	327	PF00082	0.385
CLV_PCSK_SKI1_1	338	342	PF00082	0.301
CLV_PCSK_SKI1_1	414	418	PF00082	0.449
CLV_PCSK_SKI1_1	436	440	PF00082	0.633
CLV_PCSK_SKI1_1	453	457	PF00082	0.425
CLV_PCSK_SKI1_1	466	470	PF00082	0.572
CLV_PCSK_SKI1_1	574	578	PF00082	0.616
CLV_PCSK_SKI1_1	589	593	PF00082	0.491
CLV_PCSK_SKI1_1	608	612	PF00082	0.546
CLV_PCSK_SKI1_1	7	11	PF00082	0.411
CLV_PCSK_SKI1_1	747	751	PF00082	0.639
CLV_PCSK_SKI1_1	914	918	PF00082	0.411
CLV_PCSK_SKI1_1	923	927	PF00082	0.411
DEG_APCC_DBOX_1	413	421	PF00400	0.411
DEG_APCC_DBOX_1	726	734	PF00400	0.649
DEG_Nend_UBRbox_2	1	3	PF02207	0.502
DEG_SCF_FBW7_1	610	615	PF00400	0.625
DEG_SPOP_SBC_1	803	807	PF00917	0.711
DEG_SPOP_SBC_1	868	872	PF00917	0.825
DOC_ANK_TNKS_1	509	516	PF00023	0.584
DOC_ANK_TNKS_1	622	629	PF00023	0.627
DOC_CDC14_PxL_1	147	155	PF14671	0.280
DOC_CYCLIN_RxL_1	451	461	PF00134	0.584
DOC_CYCLIN_RxL_1	568	581	PF00134	0.719
DOC_CYCLIN_yCln2_LP_2	189	195	PF00134	0.411
DOC_CYCLIN_yCln2_LP_2	816	822	PF00134	0.650
DOC_MAPK_gen_1	380	387	PF00069	0.411
DOC_MAPK_gen_1	453	459	PF00069	0.624
DOC_MAPK_gen_1	475	483	PF00069	0.690
DOC_MAPK_gen_1	561	571	PF00069	0.713
DOC_MAPK_gen_1	923	931	PF00069	0.411
DOC_MAPK_MEF2A_6	564	571	PF00069	0.706
DOC_MAPK_MEF2A_6	888	897	PF00069	0.411
DOC_PP2B_LxvP_1	816	819	PF13499	0.655
DOC_USP7_MATH_1	44	48	PF00917	0.389
DOC_USP7_MATH_1	496	500	PF00917	0.779
DOC_USP7_MATH_1	509	513	PF00917	0.638
DOC_USP7_MATH_1	578	582	PF00917	0.643
DOC_USP7_MATH_1	622	626	PF00917	0.760
DOC_USP7_MATH_1	677	681	PF00917	0.803
DOC_USP7_MATH_1	702	706	PF00917	0.770
DOC_USP7_MATH_1	794	798	PF00917	0.691
DOC_USP7_MATH_1	956	960	PF00917	0.619
DOC_USP7_UBL2_3	557	561	PF12436	0.636
DOC_WW_Pin1_4	422	427	PF00397	0.424
DOC_WW_Pin1_4	445	450	PF00397	0.513
DOC_WW_Pin1_4	520	525	PF00397	0.822
DOC_WW_Pin1_4	608	613	PF00397	0.758
DOC_WW_Pin1_4	869	874	PF00397	0.740
DOC_WW_Pin1_4	964	969	PF00397	0.571
LIG_14-3-3_CanoR_1	141	146	PF00244	0.397
LIG_14-3-3_CanoR_1	298	307	PF00244	0.459
LIG_14-3-3_CanoR_1	338	343	PF00244	0.436
LIG_14-3-3_CanoR_1	403	408	PF00244	0.338
LIG_14-3-3_CanoR_1	466	474	PF00244	0.606
LIG_14-3-3_CanoR_1	510	514	PF00244	0.584
LIG_14-3-3_CanoR_1	621	629	PF00244	0.783
LIG_14-3-3_CanoR_1	64	70	PF00244	0.389
LIG_14-3-3_CanoR_1	641	651	PF00244	0.472
LIG_14-3-3_CanoR_1	669	676	PF00244	0.586
LIG_14-3-3_CanoR_1	7	12	PF00244	0.411
LIG_14-3-3_CanoR_1	727	735	PF00244	0.745
LIG_14-3-3_CanoR_1	866	876	PF00244	0.818
LIG_Actin_WH2_2	580	595	PF00022	0.620
LIG_Actin_WH2_2	970	988	PF00022	0.514
LIG_APCC_ABBA_1	385	390	PF00400	0.411
LIG_APCC_ABBAyCdc20_2	384	390	PF00400	0.411
LIG_APCC_ABBAyCdc20_2	478	484	PF00400	0.772
LIG_BIR_III_4	850	854	PF00653	0.750
LIG_BRCT_BRCA1_1	529	533	PF00533	0.685
LIG_BRCT_BRCA1_1	784	788	PF00533	0.602
LIG_BRCT_BRCA1_2	784	790	PF00533	0.600
LIG_Clathr_ClatBox_1	456	460	PF01394	0.586
LIG_deltaCOP1_diTrp_1	686	692	PF00928	0.600
LIG_FHA_1	101	107	PF00498	0.513
LIG_FHA_1	131	137	PF00498	0.455
LIG_FHA_1	241	247	PF00498	0.411
LIG_FHA_1	300	306	PF00498	0.464
LIG_FHA_1	439	445	PF00498	0.636
LIG_FHA_1	478	484	PF00498	0.677
LIG_FHA_1	509	515	PF00498	0.746
LIG_FHA_1	521	527	PF00498	0.686
LIG_FHA_1	748	754	PF00498	0.559
LIG_FHA_1	807	813	PF00498	0.676
LIG_FHA_1	965	971	PF00498	0.532
LIG_FHA_1	985	991	PF00498	0.286
LIG_FHA_2	790	796	PF00498	0.742
LIG_LIR_Apic_2	159	164	PF02991	0.411
LIG_LIR_Gen_1	241	250	PF02991	0.411
LIG_LIR_Gen_1	252	261	PF02991	0.411
LIG_LIR_Gen_1	532	542	PF02991	0.577
LIG_LIR_Gen_1	737	745	PF02991	0.673
LIG_LIR_Gen_1	828	834	PF02991	0.621
LIG_LIR_Gen_1	84	93	PF02991	0.411
LIG_LIR_Nem_3	178	182	PF02991	0.367
LIG_LIR_Nem_3	241	245	PF02991	0.411
LIG_LIR_Nem_3	248	253	PF02991	0.411
LIG_LIR_Nem_3	530	536	PF02991	0.610
LIG_LIR_Nem_3	539	545	PF02991	0.523
LIG_LIR_Nem_3	737	741	PF02991	0.647
LIG_LIR_Nem_3	828	833	PF02991	0.621
LIG_LIR_Nem_3	84	88	PF02991	0.411
LIG_LIR_Nem_3	891	895	PF02991	0.411
LIG_MYND_1	151	155	PF01753	0.280
LIG_NRBOX	259	265	PF00104	0.411
LIG_PCNA_APIM_2	587	593	PF02747	0.608
LIG_PCNA_PIPBox_1	413	422	PF02747	0.411
LIG_PCNA_yPIPBox_3	557	567	PF02747	0.624
LIG_PCNA_yPIPBox_3	641	655	PF02747	0.795
LIG_Pex14_1	295	299	PF04695	0.488
LIG_Pex14_1	901	905	PF04695	0.411
LIG_SH2_CRK	187	191	PF00017	0.389
LIG_SH2_CRK	738	742	PF00017	0.642
LIG_SH2_GRB2like	138	141	PF00017	0.411
LIG_SH2_GRB2like	542	545	PF00017	0.579
LIG_SH2_STAP1	242	246	PF00017	0.411
LIG_SH2_STAT3	37	40	PF00017	0.513
LIG_SH2_STAT5	138	141	PF00017	0.353
LIG_SH2_STAT5	161	164	PF00017	0.360
LIG_SH2_STAT5	242	245	PF00017	0.411
LIG_SH2_STAT5	282	285	PF00017	0.527
LIG_SH2_STAT5	37	40	PF00017	0.513
LIG_SH2_STAT5	388	391	PF00017	0.411
LIG_SH2_STAT5	590	593	PF00017	0.607
LIG_SH2_STAT5	670	673	PF00017	0.706
LIG_SH2_STAT5	918	921	PF00017	0.411
LIG_SH2_STAT5	969	972	PF00017	0.522
LIG_SH2_STAT5	989	992	PF00017	0.290
LIG_SH3_3	145	151	PF00018	0.411
LIG_SH3_3	271	277	PF00018	0.411
LIG_SH3_3	423	429	PF00018	0.357
LIG_SH3_3	710	716	PF00018	0.831
LIG_SH3_3	892	898	PF00018	0.411
LIG_SH3_3	903	909	PF00018	0.411
LIG_SH3_3	949	955	PF00018	0.626
LIG_SUMO_SIM_anti_2	259	265	PF11976	0.411
LIG_SUMO_SIM_anti_2	374	379	PF11976	0.416
LIG_SUMO_SIM_anti_2	828	838	PF11976	0.735
LIG_SUMO_SIM_par_1	355	360	PF11976	0.375
LIG_SUMO_SIM_par_1	455	461	PF11976	0.582
LIG_TRAF2_1	715	718	PF00917	0.704
LIG_TRAF2_1	740	743	PF00917	0.629
LIG_TYR_ITIM	370	375	PF00017	0.411
LIG_TYR_ITIM	980	985	PF00017	0.606
LIG_UBA3_1	549	558	PF00899	0.665
LIG_WRC_WIRS_1	82	87	PF05994	0.411
MOD_CK1_1	25	31	PF00069	0.411
MOD_CK1_1	412	418	PF00069	0.513
MOD_CK1_1	458	464	PF00069	0.618
MOD_CK1_1	613	619	PF00069	0.794
MOD_CK1_1	639	645	PF00069	0.738
MOD_CK1_1	678	684	PF00069	0.802
MOD_CK1_1	696	702	PF00069	0.747
MOD_CK1_1	768	774	PF00069	0.779
MOD_CK1_1	789	795	PF00069	0.759
MOD_CK1_1	799	805	PF00069	0.669
MOD_CK1_1	832	838	PF00069	0.643
MOD_CK2_1	211	217	PF00069	0.448
MOD_CK2_1	496	502	PF00069	0.756
MOD_CK2_1	737	743	PF00069	0.747
MOD_CMANNOS	689	692	PF00535	0.723
MOD_GlcNHglycan	24	27	PF01048	0.411
MOD_GlcNHglycan	40	43	PF01048	0.411
MOD_GlcNHglycan	612	615	PF01048	0.825
MOD_GlcNHglycan	624	627	PF01048	0.601
MOD_GlcNHglycan	636	641	PF01048	0.588
MOD_GlcNHglycan	642	645	PF01048	0.517
MOD_GlcNHglycan	673	676	PF01048	0.822
MOD_GlcNHglycan	680	683	PF01048	0.710
MOD_GlcNHglycan	698	701	PF01048	0.718
MOD_GlcNHglycan	702	705	PF01048	0.720
MOD_GlcNHglycan	776	779	PF01048	0.778
MOD_GlcNHglycan	801	804	PF01048	0.761
MOD_GlcNHglycan	941	944	PF01048	0.698
MOD_GlcNHglycan	960	963	PF01048	0.642
MOD_GSK3_1	126	133	PF00069	0.457
MOD_GSK3_1	229	236	PF00069	0.507
MOD_GSK3_1	299	306	PF00069	0.459
MOD_GSK3_1	434	441	PF00069	0.627
MOD_GSK3_1	473	480	PF00069	0.721
MOD_GSK3_1	606	613	PF00069	0.790
MOD_GSK3_1	636	643	PF00069	0.797
MOD_GSK3_1	65	72	PF00069	0.411
MOD_GSK3_1	671	678	PF00069	0.812
MOD_GSK3_1	696	703	PF00069	0.759
MOD_GSK3_1	774	781	PF00069	0.800
MOD_GSK3_1	782	789	PF00069	0.675
MOD_GSK3_1	799	806	PF00069	0.717
MOD_GSK3_1	829	836	PF00069	0.624
MOD_GSK3_1	956	963	PF00069	0.759
MOD_GSK3_1	984	991	PF00069	0.410
MOD_N-GLC_1	484	489	PF02516	0.724
MOD_N-GLC_1	543	548	PF02516	0.564
MOD_N-GLC_1	728	733	PF02516	0.745
MOD_N-GLC_1	778	783	PF02516	0.800
MOD_N-GLC_1	799	804	PF02516	0.727
MOD_N-GLC_2	278	280	PF02516	0.411
MOD_NEK2_1	1	6	PF00069	0.543
MOD_NEK2_1	198	203	PF00069	0.438
MOD_NEK2_1	378	383	PF00069	0.411
MOD_NEK2_1	38	43	PF00069	0.515
MOD_NEK2_1	553	558	PF00069	0.640
MOD_NEK2_2	477	482	PF00069	0.769
MOD_NEK2_2	57	62	PF00069	0.411
MOD_PIKK_1	1	7	PF00454	0.523
MOD_PIKK_1	198	204	PF00454	0.413
MOD_PIKK_1	211	217	PF00454	0.276
MOD_PIKK_1	25	31	PF00454	0.411
MOD_PIKK_1	325	331	PF00454	0.436
MOD_PIKK_1	391	397	PF00454	0.513
MOD_PIKK_1	658	664	PF00454	0.779
MOD_PIKK_1	771	777	PF00454	0.743
MOD_PIKK_1	956	962	PF00454	0.698
MOD_PK_1	403	409	PF00069	0.411
MOD_PKA_1	466	472	PF00069	0.616
MOD_PKA_2	466	472	PF00069	0.602
MOD_PKA_2	477	483	PF00069	0.767
MOD_PKA_2	509	515	PF00069	0.582
MOD_PKA_2	616	622	PF00069	0.752
MOD_PKA_2	640	646	PF00069	0.691
MOD_PKA_2	65	71	PF00069	0.389
MOD_PKA_2	668	674	PF00069	0.704
MOD_PKA_2	696	702	PF00069	0.765
MOD_PKA_2	771	777	PF00069	0.779
MOD_PKB_1	62	70	PF00069	0.411
MOD_PKB_1	784	792	PF00069	0.597
MOD_Plk_1	1	7	PF00069	0.547
MOD_Plk_1	240	246	PF00069	0.411
MOD_Plk_1	794	800	PF00069	0.714
MOD_Plk_1	877	883	PF00069	0.280
MOD_Plk_4	338	344	PF00069	0.436
MOD_Plk_4	44	50	PF00069	0.436
MOD_Plk_4	509	515	PF00069	0.738
MOD_Plk_4	529	535	PF00069	0.515
MOD_Plk_4	808	814	PF00069	0.719
MOD_Plk_4	829	835	PF00069	0.623
MOD_Plk_4	947	953	PF00069	0.729
MOD_ProDKin_1	422	428	PF00069	0.424
MOD_ProDKin_1	445	451	PF00069	0.508
MOD_ProDKin_1	520	526	PF00069	0.819
MOD_ProDKin_1	608	614	PF00069	0.762
MOD_ProDKin_1	869	875	PF00069	0.738
MOD_ProDKin_1	964	970	PF00069	0.565
MOD_SUMO_for_1	551	554	PF00179	0.759
MOD_SUMO_rev_2	911	916	PF00179	0.411
MOD_SUMO_rev_2	978	988	PF00179	0.511
TRG_DiLeu_BaEn_1	259	264	PF01217	0.411
TRG_DiLeu_BaEn_1	829	834	PF01217	0.619
TRG_DiLeu_BaEn_2	240	246	PF01217	0.411
TRG_ENDOCYTIC_2	187	190	PF00928	0.411
TRG_ENDOCYTIC_2	242	245	PF00928	0.411
TRG_ENDOCYTIC_2	372	375	PF00928	0.411
TRG_ENDOCYTIC_2	388	391	PF00928	0.411
TRG_ENDOCYTIC_2	542	545	PF00928	0.562
TRG_ENDOCYTIC_2	738	741	PF00928	0.644
TRG_ENDOCYTIC_2	839	842	PF00928	0.696
TRG_ENDOCYTIC_2	918	921	PF00928	0.411
TRG_ENDOCYTIC_2	982	985	PF00928	0.608
TRG_ER_diArg_1	116	118	PF00400	0.443
TRG_ER_diArg_1	363	366	PF00400	0.411
TRG_ER_diArg_1	380	383	PF00400	0.411
TRG_ER_diArg_1	61	64	PF00400	0.280
TRG_ER_diArg_1	770	773	PF00400	0.779
TRG_ER_diArg_1	784	787	PF00400	0.712
TRG_ER_diArg_1	907	910	PF00400	0.411
TRG_ER_diArg_1	93	95	PF00400	0.397
TRG_NLS_MonoExtC_3	295	300	PF00514	0.492
TRG_NLS_MonoExtN_4	617	624	PF00514	0.764
TRG_Pf-PMV_PEXEL_1	168	172	PF00026	0.411
TRG_Pf-PMV_PEXEL_1	825	829	PF00026	0.616

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P480	Leptomonas seymouri	65%	86%
A0A3Q8I8J3	Leishmania donovani	94%	100%
A4H5P1	Leishmania braziliensis	84%	86%
A4HTY0	Leishmania infantum	94%	100%
E9AMR0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	90%	100%