tRNA(Phe) 7-((3-amino-3-carboxypropyl)-4-demethylwyosine(37)-N(4))-methyltransferase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Methyltransferase TYW3, putative

Species:

Leishmania major

UniProt:

Q4QHV8_LEIMA

TriTrypDb:

LmjF.09.0660 , LMJLV39_090012400 , LMJSD75_090012100

Length:

352

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 13
NetGPI	no	yes: 0, no: 13

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	3
GO:0110165	cellular anatomical entity	1	3

Expansion

Sequence features

Q4QHV8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QHV8

Function

Biological processes
Term	Name	Level	Count
GO:0001510	RNA methylation	4	3
GO:0006139	nucleobase-containing compound metabolic process	3	14
GO:0006396	RNA processing	6	14
GO:0006399	tRNA metabolic process	7	14
GO:0006400	tRNA modification	6	3
GO:0006725	cellular aromatic compound metabolic process	3	14
GO:0006807	nitrogen compound metabolic process	2	14
GO:0008033	tRNA processing	8	14
GO:0008152	metabolic process	1	14
GO:0009451	RNA modification	5	3
GO:0009987	cellular process	1	14
GO:0016070	RNA metabolic process	5	14
GO:0030488	tRNA methylation	5	3
GO:0032259	methylation	2	14
GO:0034470	ncRNA processing	7	14
GO:0034641	cellular nitrogen compound metabolic process	3	14
GO:0034660	ncRNA metabolic process	6	14
GO:0043170	macromolecule metabolic process	3	14
GO:0043412	macromolecule modification	4	3
GO:0043414	macromolecule methylation	3	3
GO:0044237	cellular metabolic process	2	14
GO:0044238	primary metabolic process	2	14
GO:0044260	obsolete cellular macromolecule metabolic process	3	3
GO:0046483	heterocycle metabolic process	3	14
GO:0071704	organic substance metabolic process	2	14
GO:0090304	nucleic acid metabolic process	4	14
GO:1901360	organic cyclic compound metabolic process	3	14

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	14
GO:0008168	methyltransferase activity	4	14
GO:0008173	RNA methyltransferase activity	4	3
GO:0008175	tRNA methyltransferase activity	5	3
GO:0016740	transferase activity	2	14
GO:0016741	transferase activity, transferring one-carbon groups	3	14
GO:0140098	catalytic activity, acting on RNA	3	3
GO:0140101	catalytic activity, acting on a tRNA	4	3
GO:0140640	catalytic activity, acting on a nucleic acid	2	3

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_MEL_PAP_1	225	231	PF00089	0.159
CLV_NRD_NRD_1	31	33	PF00675	0.662
CLV_PCSK_FUR_1	139	143	PF00082	0.408
CLV_PCSK_KEX2_1	141	143	PF00082	0.256
CLV_PCSK_PC1ET2_1	141	143	PF00082	0.259
CLV_PCSK_SKI1_1	114	118	PF00082	0.304
CLV_PCSK_SKI1_1	265	269	PF00082	0.203
CLV_PCSK_SKI1_1	336	340	PF00082	0.216
DEG_SCF_TRCP1_1	274	280	PF00400	0.318
DEG_SPOP_SBC_1	106	110	PF00917	0.307
DOC_CKS1_1	174	179	PF01111	0.354
DOC_CYCLIN_RxL_1	259	269	PF00134	0.340
DOC_CYCLIN_yCln2_LP_2	301	307	PF00134	0.248
DOC_MAPK_MEF2A_6	242	249	PF00069	0.368
DOC_MAPK_MEF2A_6	294	303	PF00069	0.240
DOC_PP2B_LxvP_1	22	25	PF13499	0.488
DOC_PP2B_LxvP_1	237	240	PF13499	0.374
DOC_PP2B_LxvP_1	301	304	PF13499	0.240
DOC_PP2B_LxvP_1	45	48	PF13499	0.652
DOC_USP7_MATH_1	181	185	PF00917	0.255
DOC_USP7_MATH_1	195	199	PF00917	0.339
DOC_USP7_MATH_1	227	231	PF00917	0.434
DOC_USP7_MATH_1	270	274	PF00917	0.261
DOC_USP7_MATH_1	84	88	PF00917	0.465
DOC_USP7_UBL2_3	137	141	PF12436	0.207
DOC_USP7_UBL2_3	29	33	PF12436	0.617
DOC_USP7_UBL2_3	58	62	PF12436	0.429
DOC_WW_Pin1_4	132	137	PF00397	0.394
DOC_WW_Pin1_4	173	178	PF00397	0.312
DOC_WW_Pin1_4	80	85	PF00397	0.227
LIG_14-3-3_CanoR_1	242	248	PF00244	0.363
LIG_BIR_II_1	1	5	PF00653	0.694
LIG_BIR_III_2	18	22	PF00653	0.458
LIG_BIR_III_2	3	7	PF00653	0.459
LIG_CtBP_PxDLS_1	160	164	PF00389	0.250
LIG_FHA_1	12	18	PF00498	0.705
LIG_FHA_1	242	248	PF00498	0.334
LIG_FHA_2	173	179	PF00498	0.350
LIG_FHA_2	99	105	PF00498	0.262
LIG_LYPXL_SIV_4	318	326	PF13949	0.354
LIG_PCNA_PIPBox_1	163	172	PF02747	0.374
LIG_PDZ_Class_2	347	352	PF00595	0.502
LIG_Rb_pABgroove_1	210	218	PF01858	0.205
LIG_SH2_STAT5	105	108	PF00017	0.267
LIG_SH2_STAT5	319	322	PF00017	0.251
LIG_SH3_3	14	20	PF00018	0.594
LIG_SH3_3	171	177	PF00018	0.347
LIG_SH3_3	25	31	PF00018	0.532
LIG_SH3_3	279	285	PF00018	0.238
LIG_SH3_4	137	144	PF00018	0.190
LIG_SH3_4	29	36	PF00018	0.695
LIG_SUMO_SIM_par_1	302	308	PF11976	0.225
LIG_UBA3_1	117	122	PF00899	0.354
LIG_UBA3_1	323	330	PF00899	0.227
LIG_WW_2	20	23	PF00397	0.451
LIG_WW_3	29	33	PF00397	0.675
LIG_WW_3	34	38	PF00397	0.697
MOD_CDK_SPK_2	132	137	PF00069	0.365
MOD_CDK_SPK_2	80	85	PF00069	0.227
MOD_CDK_SPxxK_3	132	139	PF00069	0.381
MOD_CK1_1	109	115	PF00069	0.241
MOD_CK2_1	172	178	PF00069	0.410
MOD_CK2_1	227	233	PF00069	0.419
MOD_Cter_Amidation	285	288	PF01082	0.252
MOD_GlcNHglycan	111	114	PF01048	0.227
MOD_GlcNHglycan	183	186	PF01048	0.371
MOD_GlcNHglycan	199	202	PF01048	0.368
MOD_GlcNHglycan	204	207	PF01048	0.368
MOD_GlcNHglycan	229	232	PF01048	0.235
MOD_GlcNHglycan	268	271	PF01048	0.273
MOD_GlcNHglycan	274	277	PF01048	0.298
MOD_GlcNHglycan	279	282	PF01048	0.323
MOD_GlcNHglycan	294	297	PF01048	0.322
MOD_GSK3_1	105	112	PF00069	0.282
MOD_GSK3_1	117	124	PF00069	0.246
MOD_GSK3_1	193	200	PF00069	0.337
MOD_GSK3_1	266	273	PF00069	0.257
MOD_GSK3_1	70	77	PF00069	0.378
MOD_GSK3_1	80	87	PF00069	0.360
MOD_N-GLC_2	78	80	PF02516	0.240
MOD_NEK2_1	117	122	PF00069	0.208
MOD_NEK2_1	266	271	PF00069	0.321
MOD_NEK2_1	277	282	PF00069	0.208
MOD_NEK2_1	341	346	PF00069	0.348
MOD_NEK2_2	243	248	PF00069	0.258
MOD_PIKK_1	312	318	PF00454	0.416
MOD_PKA_2	227	233	PF00069	0.340
MOD_PKA_2	241	247	PF00069	0.328
MOD_PKA_2	277	283	PF00069	0.247
MOD_Plk_1	341	347	PF00069	0.320
MOD_Plk_4	207	213	PF00069	0.215
MOD_ProDKin_1	132	138	PF00069	0.394
MOD_ProDKin_1	173	179	PF00069	0.312
MOD_ProDKin_1	80	86	PF00069	0.227
MOD_SUMO_for_1	247	250	PF00179	0.259
MOD_SUMO_rev_2	329	338	PF00179	0.316
MOD_SUMO_rev_2	83	93	PF00179	0.258
TRG_DiLeu_BaEn_1	262	267	PF01217	0.250
TRG_DiLeu_BaLyEn_6	41	46	PF01217	0.527
TRG_NLS_MonoExtC_3	31	36	PF00514	0.687
TRG_NLS_MonoExtN_4	29	36	PF00514	0.661

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P754	Leptomonas seymouri	65%	100%
A0A0S4JMK0	Bodo saltans	44%	100%
A0A1X0NNC3	Trypanosomatidae	50%	100%
A0A3Q8IBW6	Leishmania donovani	92%	100%
A0A3S5IRY7	Trypanosoma rangeli	47%	100%
A4H5P7	Leishmania braziliensis	79%	100%
A4HTY7	Leishmania infantum	92%	100%
D0A9E3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	47%	99%
E9AMR7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	89%	100%
P53177	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	24%	100%
Q4Q4P7	Leishmania major	28%	99%
V5DJH9	Trypanosoma cruzi	46%	91%
V5DMF2	Trypanosoma cruzi	28%	100%