Putative translation initiation factor EIF-2B gamma subunit

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

translation initiation factor EIF-2B gamma subunit, putative

Species:

Leishmania major

UniProt:

Q4QHR6_LEIMA

TriTrypDb:

LmjF.09.1090 , LMJLV39_090018200 , LMJSD75_090017800

Length:

601

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 4
Forrest at al. (procyclic)	no	yes: 4
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 27

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 12

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005850	eukaryotic translation initiation factor 2 complex	2	3
GO:0032991	protein-containing complex	1	3
GO:0005737	cytoplasm	2	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

Q4QHR6

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QHR6

Function

Biological processes
Term	Name	Level	Count
GO:0001731	formation of translation preinitiation complex	7	3
GO:0006417	regulation of translation	6	3
GO:0006450	regulation of translational fidelity	3	3
GO:0009889	regulation of biosynthetic process	4	3
GO:0009891	positive regulation of biosynthetic process	5	3
GO:0009893	positive regulation of metabolic process	4	3
GO:0009987	cellular process	1	3
GO:0010468	regulation of gene expression	5	3
GO:0010556	regulation of macromolecule biosynthetic process	5	3
GO:0010557	positive regulation of macromolecule biosynthetic process	6	3
GO:0010604	positive regulation of macromolecule metabolic process	5	3
GO:0010608	post-transcriptional regulation of gene expression	6	3
GO:0010628	positive regulation of gene expression	6	3
GO:0016043	cellular component organization	3	3
GO:0019222	regulation of metabolic process	3	3
GO:0022607	cellular component assembly	4	3
GO:0022618	ribonucleoprotein complex assembly	6	3
GO:0031323	regulation of cellular metabolic process	4	3
GO:0031325	positive regulation of cellular metabolic process	5	3
GO:0031326	regulation of cellular biosynthetic process	5	3
GO:0031328	positive regulation of cellular biosynthetic process	6	3
GO:0034248	regulation of amide metabolic process	5	3
GO:0034250	positive regulation of amide metabolic process	6	3
GO:0043933	protein-containing complex organization	4	3
GO:0045727	positive regulation of translation	7	3
GO:0045903	positive regulation of translational fidelity	4	3
GO:0048518	positive regulation of biological process	3	3
GO:0048522	positive regulation of cellular process	4	3
GO:0050789	regulation of biological process	2	3
GO:0050794	regulation of cellular process	3	3
GO:0051171	regulation of nitrogen compound metabolic process	4	3
GO:0051173	positive regulation of nitrogen compound metabolic process	5	3
GO:0051246	regulation of protein metabolic process	5	3
GO:0051247	positive regulation of protein metabolic process	6	3
GO:0060255	regulation of macromolecule metabolic process	4	3
GO:0065003	protein-containing complex assembly	5	3
GO:0065007	biological regulation	1	3
GO:0065008	regulation of biological quality	2	3
GO:0071826	ribonucleoprotein complex subunit organization	5	3
GO:0071840	cellular component organization or biogenesis	2	3
GO:0080090	regulation of primary metabolic process	4	3
GO:2000112	obsolete regulation of cellular macromolecule biosynthetic process	6	3

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	11
GO:0003676	nucleic acid binding	3	11
GO:0003743	translation initiation factor activity	4	11
GO:0003824	catalytic activity	1	11
GO:0003924	GTPase activity	7	11
GO:0005488	binding	1	11
GO:0005525	GTP binding	5	11
GO:0008135	translation factor activity, RNA binding	3	11
GO:0016462	pyrophosphatase activity	5	11
GO:0016787	hydrolase activity	2	11
GO:0016817	hydrolase activity, acting on acid anhydrides	3	11
GO:0016818	hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	4	11
GO:0017076	purine nucleotide binding	4	11
GO:0017111	ribonucleoside triphosphate phosphatase activity	6	11
GO:0019001	guanyl nucleotide binding	5	11
GO:0032553	ribonucleotide binding	3	11
GO:0032555	purine ribonucleotide binding	4	11
GO:0032561	guanyl ribonucleotide binding	5	11
GO:0035639	purine ribonucleoside triphosphate binding	4	11
GO:0036094	small molecule binding	2	11
GO:0043167	ion binding	2	11
GO:0043168	anion binding	3	11
GO:0045182	translation regulator activity	1	11
GO:0090079	translation regulator activity, nucleic acid binding	2	11
GO:0097159	organic cyclic compound binding	2	11
GO:0097367	carbohydrate derivative binding	2	11
GO:1901265	nucleoside phosphate binding	3	11
GO:1901363	heterocyclic compound binding	2	11
GO:0000049	tRNA binding	5	6
GO:0003723	RNA binding	4	6
GO:0003746	translation elongation factor activity	4	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	114	118	PF00656	0.664
CLV_C14_Caspase3-7	430	434	PF00656	0.656
CLV_NRD_NRD_1	251	253	PF00675	0.481
CLV_NRD_NRD_1	312	314	PF00675	0.582
CLV_NRD_NRD_1	319	321	PF00675	0.538
CLV_NRD_NRD_1	401	403	PF00675	0.603
CLV_NRD_NRD_1	408	410	PF00675	0.616
CLV_NRD_NRD_1	518	520	PF00675	0.386
CLV_NRD_NRD_1	576	578	PF00675	0.423
CLV_PCSK_FUR_1	399	403	PF00082	0.551
CLV_PCSK_FUR_1	406	410	PF00082	0.578
CLV_PCSK_KEX2_1	131	133	PF00082	0.388
CLV_PCSK_KEX2_1	251	253	PF00082	0.494
CLV_PCSK_KEX2_1	311	313	PF00082	0.567
CLV_PCSK_KEX2_1	319	321	PF00082	0.530
CLV_PCSK_KEX2_1	401	403	PF00082	0.603
CLV_PCSK_KEX2_1	408	410	PF00082	0.616
CLV_PCSK_KEX2_1	518	520	PF00082	0.561
CLV_PCSK_PC1ET2_1	131	133	PF00082	0.388
CLV_PCSK_SKI1_1	279	283	PF00082	0.554
CLV_PCSK_SKI1_1	46	50	PF00082	0.515
CLV_PCSK_SKI1_1	568	572	PF00082	0.392
DEG_SPOP_SBC_1	426	430	PF00917	0.643
DOC_CDC14_PxL_1	301	309	PF14671	0.441
DOC_CYCLIN_yCln2_LP_2	556	562	PF00134	0.386
DOC_MAPK_gen_1	383	391	PF00069	0.484
DOC_MAPK_gen_1	76	85	PF00069	0.588
DOC_MAPK_MEF2A_6	524	533	PF00069	0.392
DOC_MAPK_MEF2A_6	79	87	PF00069	0.588
DOC_MAPK_RevD_3	305	320	PF00069	0.529
DOC_PP1_RVXF_1	578	585	PF00149	0.453
DOC_PP2B_LxvP_1	480	483	PF13499	0.532
DOC_PP4_FxxP_1	169	172	PF00568	0.588
DOC_PP4_FxxP_1	361	364	PF00568	0.441
DOC_USP7_MATH_1	165	169	PF00917	0.664
DOC_USP7_MATH_1	420	424	PF00917	0.718
DOC_WW_Pin1_4	116	121	PF00397	0.590
DOC_WW_Pin1_4	331	336	PF00397	0.687
DOC_WW_Pin1_4	409	414	PF00397	0.660
DOC_WW_Pin1_4	422	427	PF00397	0.589
DOC_WW_Pin1_4	434	439	PF00397	0.620
DOC_WW_Pin1_4	444	449	PF00397	0.586
DOC_WW_Pin1_4	555	560	PF00397	0.442
LIG_14-3-3_CanoR_1	132	137	PF00244	0.588
LIG_14-3-3_CanoR_1	346	350	PF00244	0.535
LIG_14-3-3_CanoR_1	511	517	PF00244	0.386
LIG_Actin_WH2_2	460	477	PF00022	0.468
LIG_Actin_WH2_2	529	546	PF00022	0.392
LIG_Actin_WH2_2	575	591	PF00022	0.339
LIG_APCC_ABBA_1	461	466	PF00400	0.464
LIG_BIR_II_1	1	5	PF00653	0.723
LIG_CSL_BTD_1	435	438	PF09270	0.583
LIG_FHA_1	125	131	PF00498	0.596
LIG_FHA_1	345	351	PF00498	0.591
LIG_FHA_1	38	44	PF00498	0.561
LIG_FHA_1	422	428	PF00498	0.703
LIG_FHA_1	47	53	PF00498	0.460
LIG_FHA_1	84	90	PF00498	0.588
LIG_FHA_2	370	376	PF00498	0.541
LIG_FHA_2	428	434	PF00498	0.655
LIG_FHA_2	8	14	PF00498	0.740
LIG_LIR_Apic_2	167	172	PF02991	0.588
LIG_LIR_Apic_2	339	344	PF02991	0.496
LIG_LIR_Apic_2	358	364	PF02991	0.463
LIG_LIR_Gen_1	27	36	PF02991	0.658
LIG_LIR_Gen_1	39	48	PF02991	0.488
LIG_LIR_Nem_3	106	111	PF02991	0.664
LIG_LIR_Nem_3	27	31	PF02991	0.610
LIG_PCNA_PIPBox_1	207	216	PF02747	0.547
LIG_PCNA_PIPBox_1	465	474	PF02747	0.442
LIG_PCNA_yPIPBox_3	465	475	PF02747	0.434
LIG_REV1ctd_RIR_1	469	479	PF16727	0.471
LIG_SH2_CRK	208	212	PF00017	0.548
LIG_SH2_CRK	259	263	PF00017	0.394
LIG_SH2_CRK	341	345	PF00017	0.648
LIG_SH2_GRB2like	259	262	PF00017	0.443
LIG_SH2_GRB2like	91	94	PF00017	0.588
LIG_SH2_NCK_1	221	225	PF00017	0.539
LIG_SH2_SRC	464	467	PF00017	0.439
LIG_SH2_SRC	91	94	PF00017	0.588
LIG_SH2_STAT5	214	217	PF00017	0.588
LIG_SH2_STAT5	243	246	PF00017	0.594
LIG_SH2_STAT5	331	334	PF00017	0.618
LIG_SH2_STAT5	574	577	PF00017	0.386
LIG_SH3_3	134	140	PF00018	0.580
LIG_SH3_3	222	228	PF00018	0.588
LIG_SH3_3	242	248	PF00018	0.423
LIG_SH3_3	265	271	PF00018	0.493
LIG_SH3_3	329	335	PF00018	0.599
LIG_Sin3_3	158	165	PF02671	0.588
LIG_SUMO_SIM_anti_2	188	193	PF11976	0.550
LIG_SUMO_SIM_anti_2	299	305	PF11976	0.432
LIG_SUMO_SIM_par_1	366	373	PF11976	0.516
LIG_SUMO_SIM_par_1	525	535	PF11976	0.381
LIG_UBA3_1	189	195	PF00899	0.539
LIG_WRC_WIRS_1	263	268	PF05994	0.517
LIG_WRC_WIRS_1	38	43	PF05994	0.562
MOD_CK1_1	421	427	PF00069	0.660
MOD_CK1_1	432	438	PF00069	0.590
MOD_CK1_1	532	538	PF00069	0.386
MOD_CK1_1	558	564	PF00069	0.339
MOD_CK2_1	322	328	PF00069	0.664
MOD_GlcNHglycan	324	327	PF01048	0.639
MOD_GlcNHglycan	420	423	PF01048	0.700
MOD_GlcNHglycan	438	441	PF01048	0.536
MOD_GlcNHglycan	560	563	PF01048	0.386
MOD_GlcNHglycan	69	72	PF01048	0.356
MOD_GSK3_1	103	110	PF00069	0.570
MOD_GSK3_1	111	118	PF00069	0.542
MOD_GSK3_1	262	269	PF00069	0.498
MOD_GSK3_1	370	377	PF00069	0.654
MOD_GSK3_1	418	425	PF00069	0.728
MOD_GSK3_1	42	49	PF00069	0.466
MOD_GSK3_1	428	435	PF00069	0.770
MOD_GSK3_1	525	532	PF00069	0.478
MOD_GSK3_1	534	541	PF00069	0.434
MOD_GSK3_1	67	74	PF00069	0.568
MOD_N-GLC_1	165	170	PF02516	0.464
MOD_N-GLC_1	235	240	PF02516	0.339
MOD_N-GLC_1	83	88	PF02516	0.388
MOD_NEK2_1	145	150	PF00069	0.588
MOD_NEK2_1	266	271	PF00069	0.486
MOD_NEK2_1	345	350	PF00069	0.499
MOD_NEK2_1	543	548	PF00069	0.386
MOD_NEK2_1	83	88	PF00069	0.588
MOD_OFUCOSY	102	107	PF10250	0.464
MOD_PIKK_1	111	117	PF00454	0.554
MOD_PIKK_1	543	549	PF00454	0.456
MOD_PIKK_1	71	77	PF00454	0.588
MOD_PKA_2	103	109	PF00069	0.664
MOD_PKA_2	345	351	PF00069	0.533
MOD_PKA_2	486	492	PF00069	0.635
MOD_PKA_2	543	549	PF00069	0.386
MOD_Plk_1	165	171	PF00069	0.664
MOD_Plk_1	235	241	PF00069	0.539
MOD_Plk_1	266	272	PF00069	0.511
MOD_Plk_1	432	438	PF00069	0.589
MOD_Plk_1	525	531	PF00069	0.339
MOD_Plk_1	7	13	PF00069	0.734
MOD_Plk_1	83	89	PF00069	0.588
MOD_Plk_4	145	151	PF00069	0.588
MOD_Plk_4	185	191	PF00069	0.543
MOD_Plk_4	206	212	PF00069	0.523
MOD_Plk_4	345	351	PF00069	0.635
MOD_Plk_4	525	531	PF00069	0.420
MOD_Plk_4	538	544	PF00069	0.500
MOD_Plk_4	583	589	PF00069	0.339
MOD_Plk_4	61	67	PF00069	0.549
MOD_ProDKin_1	116	122	PF00069	0.590
MOD_ProDKin_1	331	337	PF00069	0.686
MOD_ProDKin_1	409	415	PF00069	0.662
MOD_ProDKin_1	422	428	PF00069	0.605
MOD_ProDKin_1	434	440	PF00069	0.619
MOD_ProDKin_1	444	450	PF00069	0.586
MOD_ProDKin_1	555	561	PF00069	0.442
MOD_SUMO_rev_2	8	17	PF00179	0.678
TRG_DiLeu_BaEn_3	525	531	PF01217	0.281
TRG_DiLeu_BaEn_4	526	532	PF01217	0.281
TRG_ENDOCYTIC_2	208	211	PF00928	0.601
TRG_ENDOCYTIC_2	259	262	PF00928	0.443
TRG_ENDOCYTIC_2	464	467	PF00928	0.441
TRG_ER_diArg_1	250	252	PF00400	0.501
TRG_ER_diArg_1	310	313	PF00400	0.706
TRG_ER_diArg_1	318	320	PF00400	0.594
TRG_ER_diArg_1	398	401	PF00400	0.578
TRG_ER_diArg_1	406	409	PF00400	0.603
TRG_ER_diArg_1	518	520	PF00400	0.386
TRG_NES_CRM1_1	182	193	PF08389	0.664
TRG_Pf-PMV_PEXEL_1	594	598	PF00026	0.556

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A1X0NJL3	Trypanosomatidae	55%	100%
A0A3S7WQT0	Leishmania donovani	70%	100%
A4H5T6	Leishmania braziliensis	69%	100%
A4H5T7	Leishmania braziliensis	86%	100%
A4HU29	Leishmania infantum	70%	100%
A4HU30	Leishmania infantum	95%	100%
E9AMV9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	70%	100%
E9AMW0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	95%	100%
Q4QHR7	Leishmania major	70%	100%