Thymidylate kinase-like protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

thymidylate kinase-like protein

Species:

Leishmania major

UniProt:

Q4QHL4_LEIMA

TriTrypDb:

LmjF.10.0060 , LMJLV39_100005400 , LMJSD75_100005500

Length:

363

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 13
NetGPI	no	yes: 0, no: 13

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	2
GO:0005737	cytoplasm	2	2
GO:0005739	mitochondrion	5	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

Q4QHL4

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QHL4

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	6
GO:0006220	pyrimidine nucleotide metabolic process	5	6
GO:0006221	pyrimidine nucleotide biosynthetic process	6	6
GO:0006227	dUDP biosynthetic process	8	2
GO:0006233	dTDP biosynthetic process	8	6
GO:0006235	dTTP biosynthetic process	8	2
GO:0006725	cellular aromatic compound metabolic process	3	6
GO:0006753	nucleoside phosphate metabolic process	4	6
GO:0006793	phosphorus metabolic process	3	11
GO:0006796	phosphate-containing compound metabolic process	4	11
GO:0006807	nitrogen compound metabolic process	2	6
GO:0008152	metabolic process	1	11
GO:0009058	biosynthetic process	2	6
GO:0009117	nucleotide metabolic process	5	6
GO:0009132	nucleoside diphosphate metabolic process	5	6
GO:0009133	nucleoside diphosphate biosynthetic process	6	6
GO:0009138	pyrimidine nucleoside diphosphate metabolic process	6	6
GO:0009139	pyrimidine nucleoside diphosphate biosynthetic process	7	6
GO:0009141	nucleoside triphosphate metabolic process	5	2
GO:0009142	nucleoside triphosphate biosynthetic process	6	2
GO:0009147	pyrimidine nucleoside triphosphate metabolic process	6	2
GO:0009148	pyrimidine nucleoside triphosphate biosynthetic process	7	2
GO:0009165	nucleotide biosynthetic process	6	6
GO:0009186	deoxyribonucleoside diphosphate metabolic process	6	6
GO:0009189	deoxyribonucleoside diphosphate biosynthetic process	7	6
GO:0009196	pyrimidine deoxyribonucleoside diphosphate metabolic process	7	6
GO:0009197	pyrimidine deoxyribonucleoside diphosphate biosynthetic process	8	6
GO:0009211	pyrimidine deoxyribonucleoside triphosphate metabolic process	7	2
GO:0009212	pyrimidine deoxyribonucleoside triphosphate biosynthetic process	8	2
GO:0009219	pyrimidine deoxyribonucleotide metabolic process	6	6
GO:0009221	pyrimidine deoxyribonucleotide biosynthetic process	7	6
GO:0009262	deoxyribonucleotide metabolic process	4	6
GO:0009263	deoxyribonucleotide biosynthetic process	5	6
GO:0009265	2'-deoxyribonucleotide biosynthetic process	6	6
GO:0009394	2'-deoxyribonucleotide metabolic process	5	6
GO:0009987	cellular process	1	11
GO:0016310	phosphorylation	5	11
GO:0018130	heterocycle biosynthetic process	4	6
GO:0019438	aromatic compound biosynthetic process	4	6
GO:0019637	organophosphate metabolic process	3	6
GO:0019692	deoxyribose phosphate metabolic process	4	6
GO:0034641	cellular nitrogen compound metabolic process	3	6
GO:0034654	nucleobase-containing compound biosynthetic process	4	6
GO:0044237	cellular metabolic process	2	11
GO:0044238	primary metabolic process	2	6
GO:0044249	cellular biosynthetic process	3	6
GO:0044271	cellular nitrogen compound biosynthetic process	4	6
GO:0044281	small molecule metabolic process	2	6
GO:0046072	dTDP metabolic process	7	6
GO:0046075	dTTP metabolic process	7	2
GO:0046077	dUDP metabolic process	7	2
GO:0046385	deoxyribose phosphate biosynthetic process	5	6
GO:0046483	heterocycle metabolic process	3	6
GO:0055086	nucleobase-containing small molecule metabolic process	3	6
GO:0071704	organic substance metabolic process	2	6
GO:0072527	pyrimidine-containing compound metabolic process	4	6
GO:0072528	pyrimidine-containing compound biosynthetic process	5	6
GO:0090407	organophosphate biosynthetic process	4	6
GO:1901135	carbohydrate derivative metabolic process	3	6
GO:1901137	carbohydrate derivative biosynthetic process	4	6
GO:1901293	nucleoside phosphate biosynthetic process	5	6
GO:1901360	organic cyclic compound metabolic process	3	6
GO:1901362	organic cyclic compound biosynthetic process	4	6
GO:1901564	organonitrogen compound metabolic process	3	6
GO:1901566	organonitrogen compound biosynthetic process	4	6
GO:1901576	organic substance biosynthetic process	3	6

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	11
GO:0004550	nucleoside diphosphate kinase activity	5	2
GO:0004798	thymidylate kinase activity	6	10
GO:0009041	uridylate kinase activity	6	2
GO:0016301	kinase activity	4	11
GO:0016740	transferase activity	2	11
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	11
GO:0016776	phosphotransferase activity, phosphate group as acceptor	4	10
GO:0019205	nucleobase-containing compound kinase activity	5	10
GO:0050145	nucleoside monophosphate kinase activity	5	10
GO:0000166	nucleotide binding	3	4
GO:0005488	binding	1	4
GO:0005524	ATP binding	5	4
GO:0017076	purine nucleotide binding	4	4
GO:0030554	adenyl nucleotide binding	5	4
GO:0032553	ribonucleotide binding	3	4
GO:0032555	purine ribonucleotide binding	4	4
GO:0032559	adenyl ribonucleotide binding	5	4
GO:0035639	purine ribonucleoside triphosphate binding	4	4
GO:0036094	small molecule binding	2	4
GO:0043167	ion binding	2	4
GO:0043168	anion binding	3	4
GO:0097159	organic cyclic compound binding	2	4
GO:0097367	carbohydrate derivative binding	2	4
GO:1901265	nucleoside phosphate binding	3	4
GO:1901363	heterocyclic compound binding	2	4

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	144	148	PF00656	0.343
CLV_NRD_NRD_1	120	122	PF00675	0.544
CLV_NRD_NRD_1	160	162	PF00675	0.412
CLV_NRD_NRD_1	224	226	PF00675	0.387
CLV_NRD_NRD_1	293	295	PF00675	0.411
CLV_NRD_NRD_1	336	338	PF00675	0.552
CLV_PCSK_KEX2_1	160	162	PF00082	0.441
CLV_PCSK_KEX2_1	224	226	PF00082	0.407
CLV_PCSK_KEX2_1	336	338	PF00082	0.552
CLV_PCSK_SKI1_1	186	190	PF00082	0.343
CLV_PCSK_SKI1_1	251	255	PF00082	0.449
CLV_PCSK_SKI1_1	336	340	PF00082	0.559
DEG_APCC_DBOX_1	60	68	PF00400	0.683
DEG_Nend_UBRbox_1	1	4	PF02207	0.446
DEG_SCF_FBW7_1	98	105	PF00400	0.586
DEG_SPOP_SBC_1	132	136	PF00917	0.566
DOC_CKS1_1	99	104	PF01111	0.585
DOC_MAPK_gen_1	224	231	PF00069	0.384
DOC_MAPK_MEF2A_6	270	279	PF00069	0.396
DOC_PP1_RVXF_1	215	222	PF00149	0.445
DOC_PP2B_LxvP_1	266	269	PF13499	0.301
DOC_PP2B_LxvP_1	277	280	PF13499	0.314
DOC_USP7_MATH_1	114	118	PF00917	0.607
DOC_USP7_MATH_1	124	128	PF00917	0.390
DOC_USP7_MATH_1	133	137	PF00917	0.550
DOC_USP7_MATH_1	20	24	PF00917	0.510
DOC_WW_Pin1_4	104	109	PF00397	0.595
DOC_WW_Pin1_4	53	58	PF00397	0.693
DOC_WW_Pin1_4	98	103	PF00397	0.524
LIG_14-3-3_CanoR_1	19	29	PF00244	0.442
LIG_14-3-3_CanoR_1	224	230	PF00244	0.410
LIG_14-3-3_CanoR_1	270	274	PF00244	0.449
LIG_14-3-3_CanoR_1	310	316	PF00244	0.347
LIG_APCC_ABBAyCdc20_2	2	8	PF00400	0.415
LIG_Dynein_DLC8_1	149	155	PF01221	0.336
LIG_FHA_1	153	159	PF00498	0.355
LIG_FHA_1	270	276	PF00498	0.436
LIG_FHA_1	346	352	PF00498	0.544
LIG_FHA_1	95	101	PF00498	0.475
LIG_FHA_2	337	343	PF00498	0.553
LIG_FHA_2	78	84	PF00498	0.558
LIG_KLC1_Yacidic_2	28	33	PF13176	0.550
LIG_LIR_Gen_1	23	34	PF02991	0.545
LIG_LIR_Gen_1	257	268	PF02991	0.371
LIG_LIR_Gen_1	85	95	PF02991	0.535
LIG_LIR_Nem_3	126	131	PF02991	0.463
LIG_LIR_Nem_3	23	29	PF02991	0.532
LIG_LIR_Nem_3	257	263	PF02991	0.345
LIG_LIR_Nem_3	264	268	PF02991	0.300
LIG_LIR_Nem_3	272	276	PF02991	0.271
LIG_LIR_Nem_3	70	74	PF02991	0.459
LIG_LIR_Nem_3	85	91	PF02991	0.259
LIG_PCNA_TLS_4	122	129	PF02747	0.358
LIG_PCNA_yPIPBox_3	193	207	PF02747	0.447
LIG_Pex14_1	46	50	PF04695	0.562
LIG_SH2_CRK	181	185	PF00017	0.358
LIG_SH2_PTP2	31	34	PF00017	0.522
LIG_SH2_SRC	26	29	PF00017	0.553
LIG_SH2_SRC	31	34	PF00017	0.513
LIG_SH2_STAP1	235	239	PF00017	0.421
LIG_SH2_STAP1	26	30	PF00017	0.558
LIG_SH2_STAP1	88	92	PF00017	0.469
LIG_SH2_STAT5	235	238	PF00017	0.344
LIG_SH2_STAT5	273	276	PF00017	0.359
LIG_SH2_STAT5	31	34	PF00017	0.495
LIG_SH2_STAT5	74	77	PF00017	0.492
LIG_SH3_3	96	102	PF00018	0.494
LIG_SUMO_SIM_anti_2	139	144	PF11976	0.506
LIG_SUMO_SIM_par_1	139	144	PF11976	0.452
LIG_SUMO_SIM_par_1	96	101	PF11976	0.413
LIG_TRAF2_1	247	250	PF00917	0.371
LIG_TRAF2_1	339	342	PF00917	0.538
LIG_TYR_ITIM	274	279	PF00017	0.353
LIG_TYR_ITIM	29	34	PF00017	0.524
LIG_TYR_ITSM	22	29	PF00017	0.443
LIG_WRC_WIRS_1	125	130	PF05994	0.427
LIG_WRC_WIRS_1	203	208	PF05994	0.427
MOD_CK1_1	127	133	PF00069	0.508
MOD_CK1_1	134	140	PF00069	0.502
MOD_CK1_1	346	352	PF00069	0.524
MOD_CK2_1	133	139	PF00069	0.579
MOD_CK2_1	22	28	PF00069	0.575
MOD_CK2_1	253	259	PF00069	0.444
MOD_CK2_1	336	342	PF00069	0.530
MOD_Cter_Amidation	190	193	PF01082	0.376
MOD_Cter_Amidation	334	337	PF01082	0.574
MOD_GlcNHglycan	10	13	PF01048	0.535
MOD_GlcNHglycan	118	121	PF01048	0.500
MOD_GlcNHglycan	136	139	PF01048	0.409
MOD_GlcNHglycan	189	192	PF01048	0.346
MOD_GlcNHglycan	36	39	PF01048	0.547
MOD_GSK3_1	110	117	PF00069	0.544
MOD_GSK3_1	127	134	PF00069	0.508
MOD_GSK3_1	202	209	PF00069	0.343
MOD_GSK3_1	4	11	PF00069	0.483
MOD_GSK3_1	41	48	PF00069	0.531
MOD_GSK3_1	73	80	PF00069	0.429
MOD_GSK3_1	94	101	PF00069	0.441
MOD_NEK2_1	206	211	PF00069	0.330
MOD_NEK2_1	67	72	PF00069	0.582
MOD_NEK2_2	124	129	PF00069	0.521
MOD_PIKK_1	150	156	PF00454	0.356
MOD_PK_1	225	231	PF00069	0.468
MOD_PKA_1	224	230	PF00069	0.451
MOD_PKA_1	336	342	PF00069	0.561
MOD_PKA_2	224	230	PF00069	0.408
MOD_PKA_2	269	275	PF00069	0.460
MOD_PKA_2	311	317	PF00069	0.367
MOD_PKA_2	336	342	PF00069	0.468
MOD_Plk_2-3	329	335	PF00069	0.337
MOD_Plk_2-3	77	83	PF00069	0.511
MOD_Plk_4	124	130	PF00069	0.511
MOD_Plk_4	225	231	PF00069	0.445
MOD_Plk_4	269	275	PF00069	0.423
MOD_Plk_4	67	73	PF00069	0.560
MOD_Plk_4	94	100	PF00069	0.464
MOD_ProDKin_1	53	59	PF00069	0.696
MOD_ProDKin_1	98	104	PF00069	0.541
MOD_SUMO_rev_2	62	70	PF00179	0.649
TRG_DiLeu_BaEn_1	94	99	PF01217	0.532
TRG_DiLeu_BaEn_3	249	255	PF01217	0.371
TRG_ENDOCYTIC_2	181	184	PF00928	0.330
TRG_ENDOCYTIC_2	26	29	PF00928	0.545
TRG_ENDOCYTIC_2	276	279	PF00928	0.338
TRG_ENDOCYTIC_2	31	34	PF00928	0.522
TRG_ENDOCYTIC_2	88	91	PF00928	0.443
TRG_ER_diArg_1	159	161	PF00400	0.443
TRG_ER_diArg_1	223	225	PF00400	0.421
TRG_ER_diArg_1	336	338	PF00400	0.552
TRG_Pf-PMV_PEXEL_1	336	341	PF00026	0.561

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HS08	Leptomonas seymouri	63%	100%
A0A0S4KK84	Bodo saltans	36%	96%
A0A1X0NRS1	Trypanosomatidae	49%	100%
A0A3Q8I8L2	Leishmania donovani	96%	100%
A0A3R7KQ58	Trypanosoma rangeli	51%	100%
A0A3R7LZ67	Trypanosoma rangeli	24%	90%
A4H5Y8	Leishmania braziliensis	85%	100%
A4HUB3	Leishmania infantum	96%	100%
C9ZVH7	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	43%	100%
E9AN12	Leishmania mexicana (strain MHOM/GT/2001/U1103)	93%	100%
V5AU19	Trypanosoma cruzi	44%	100%
V5BR99	Trypanosoma cruzi	26%	90%