Putative eIF-2B GDP-GTP exchange factor

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

translation initiation factor eIF-2B subunit beta, putative

Species:

Leishmania major

UniProt:

Q4QHB9_LEIMA

TriTrypDb:

LmjF.10.0950 , LMJLV39_100016400 , LMJSD75_100016600

Length:

533

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 9
NetGPI	no	yes: 0, no: 9

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	2
GO:0005851	eukaryotic translation initiation factor 2B complex	2	2
GO:0032991	protein-containing complex	1	2
GO:0110165	cellular anatomical entity	1	3
GO:0016020	membrane	2	1

Expansion

Sequence features

Q4QHB9

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QHB9

Function

Biological processes
Term	Name	Level	Count
GO:0006413	translational initiation	3	2
GO:0008152	metabolic process	1	2
GO:0009987	cellular process	1	2
GO:0044237	cellular metabolic process	2	2
GO:0050790	regulation of catalytic activity	3	2
GO:0065007	biological regulation	1	2
GO:0065009	regulation of molecular function	2	2

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	10
GO:0003743	translation initiation factor activity	4	10
GO:0005488	binding	1	10
GO:0008135	translation factor activity, RNA binding	3	10
GO:0045182	translation regulator activity	1	10
GO:0090079	translation regulator activity, nucleic acid binding	2	10
GO:0097159	organic cyclic compound binding	2	10
GO:1901363	heterocyclic compound binding	2	10
GO:0003824	catalytic activity	1	1
GO:0016853	isomerase activity	2	1
GO:0016860	intramolecular oxidoreductase activity	3	1
GO:0016861	intramolecular oxidoreductase activity, interconverting aldoses and ketoses	4	1
GO:0046523	S-methyl-5-thioribose-1-phosphate isomerase activity	5	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	295	299	PF00656	0.563
CLV_C14_Caspase3-7	483	487	PF00656	0.560
CLV_NRD_NRD_1	119	121	PF00675	0.376
CLV_NRD_NRD_1	207	209	PF00675	0.656
CLV_NRD_NRD_1	316	318	PF00675	0.356
CLV_NRD_NRD_1	338	340	PF00675	0.356
CLV_NRD_NRD_1	489	491	PF00675	0.370
CLV_PCSK_KEX2_1	119	121	PF00082	0.376
CLV_PCSK_KEX2_1	196	198	PF00082	0.692
CLV_PCSK_KEX2_1	207	209	PF00082	0.588
CLV_PCSK_KEX2_1	266	268	PF00082	0.629
CLV_PCSK_KEX2_1	28	30	PF00082	0.501
CLV_PCSK_KEX2_1	304	306	PF00082	0.347
CLV_PCSK_KEX2_1	316	318	PF00082	0.285
CLV_PCSK_KEX2_1	338	340	PF00082	0.356
CLV_PCSK_KEX2_1	489	491	PF00082	0.370
CLV_PCSK_PC1ET2_1	196	198	PF00082	0.785
CLV_PCSK_PC1ET2_1	266	268	PF00082	0.721
CLV_PCSK_PC1ET2_1	28	30	PF00082	0.575
CLV_PCSK_PC1ET2_1	304	306	PF00082	0.347
CLV_PCSK_PC7_1	262	268	PF00082	0.663
CLV_PCSK_SKI1_1	119	123	PF00082	0.386
CLV_PCSK_SKI1_1	144	148	PF00082	0.565
CLV_PCSK_SKI1_1	189	193	PF00082	0.791
CLV_PCSK_SKI1_1	262	266	PF00082	0.679
CLV_PCSK_SKI1_1	339	343	PF00082	0.356
CLV_PCSK_SKI1_1	376	380	PF00082	0.360
CLV_PCSK_SKI1_1	489	493	PF00082	0.356
DEG_APCC_DBOX_1	118	126	PF00400	0.384
DEG_Nend_Nbox_1	1	3	PF02207	0.710
DEG_SCF_FBW7_1	158	165	PF00400	0.701
DEG_SCF_TRCP1_1	486	492	PF00400	0.577
DEG_SPOP_SBC_1	163	167	PF00917	0.751
DEG_SPOP_SBC_1	215	219	PF00917	0.618
DEG_SPOP_SBC_1	433	437	PF00917	0.507
DOC_CKS1_1	159	164	PF01111	0.778
DOC_CYCLIN_RxL_1	335	343	PF00134	0.556
DOC_CYCLIN_RxL_1	89	100	PF00134	0.460
DOC_MAPK_gen_1	207	214	PF00069	0.736
DOC_MAPK_MEF2A_6	207	214	PF00069	0.736
DOC_MAPK_MEF2A_6	425	433	PF00069	0.490
DOC_PP4_FxxP_1	243	246	PF00568	0.739
DOC_PP4_FxxP_1	427	430	PF00568	0.476
DOC_USP7_MATH_1	129	133	PF00917	0.505
DOC_USP7_MATH_1	163	167	PF00917	0.751
DOC_USP7_MATH_1	182	186	PF00917	0.721
DOC_USP7_MATH_1	215	219	PF00917	0.722
DOC_USP7_MATH_1	34	38	PF00917	0.691
DOC_USP7_MATH_1	372	376	PF00917	0.529
DOC_USP7_MATH_1	384	388	PF00917	0.421
DOC_USP7_UBL2_3	192	196	PF12436	0.746
DOC_WW_Pin1_4	158	163	PF00397	0.708
DOC_WW_Pin1_4	180	185	PF00397	0.671
DOC_WW_Pin1_4	438	443	PF00397	0.490
DOC_WW_Pin1_4	468	473	PF00397	0.495
DOC_WW_Pin1_4	475	480	PF00397	0.482
LIG_14-3-3_CanoR_1	207	213	PF00244	0.665
LIG_14-3-3_CanoR_1	316	322	PF00244	0.520
LIG_14-3-3_CanoR_1	376	384	PF00244	0.560
LIG_Actin_WH2_2	12	30	PF00022	0.505
LIG_Actin_WH2_2	252	268	PF00022	0.629
LIG_Actin_WH2_2	426	441	PF00022	0.476
LIG_BRCT_BRCA1_1	210	214	PF00533	0.743
LIG_deltaCOP1_diTrp_1	473	480	PF00928	0.542
LIG_eIF4E_1	500	506	PF01652	0.476
LIG_FHA_1	109	115	PF00498	0.430
LIG_FHA_1	116	122	PF00498	0.355
LIG_FHA_1	249	255	PF00498	0.696
LIG_FHA_1	309	315	PF00498	0.513
LIG_FHA_1	317	323	PF00498	0.461
LIG_FHA_1	377	383	PF00498	0.544
LIG_FHA_1	394	400	PF00498	0.395
LIG_FHA_1	415	421	PF00498	0.476
LIG_FHA_1	433	439	PF00498	0.476
LIG_FHA_1	449	455	PF00498	0.467
LIG_FHA_1	496	502	PF00498	0.478
LIG_FHA_1	505	511	PF00498	0.481
LIG_FHA_1	70	76	PF00498	0.466
LIG_FHA_2	133	139	PF00498	0.591
LIG_FHA_2	159	165	PF00498	0.751
LIG_FHA_2	217	223	PF00498	0.671
LIG_LIR_Apic_2	241	246	PF02991	0.741
LIG_LIR_Apic_2	467	472	PF02991	0.478
LIG_LIR_Apic_2	473	479	PF02991	0.473
LIG_LIR_Apic_2	498	503	PF02991	0.476
LIG_LIR_Gen_1	386	394	PF02991	0.538
LIG_LIR_Nem_3	463	469	PF02991	0.473
LIG_MYND_2	502	506	PF01753	0.490
LIG_PCNA_PIPBox_1	54	63	PF02747	0.461
LIG_Pex14_1	476	480	PF04695	0.556
LIG_Pex14_2	109	113	PF04695	0.557
LIG_SH2_NCK_1	529	533	PF00017	0.556
LIG_SH2_PTP2	500	503	PF00017	0.476
LIG_SH2_SRC	293	296	PF00017	0.577
LIG_SH2_SRC	500	503	PF00017	0.476
LIG_SH2_STAT5	340	343	PF00017	0.490
LIG_SH2_STAT5	389	392	PF00017	0.556
LIG_SH2_STAT5	500	503	PF00017	0.464
LIG_SH3_1	439	445	PF00018	0.490
LIG_SH3_2	6	11	PF14604	0.449
LIG_SH3_3	156	162	PF00018	0.709
LIG_SH3_3	178	184	PF00018	0.754
LIG_SH3_3	198	204	PF00018	0.776
LIG_SH3_3	3	9	PF00018	0.533
LIG_SH3_3	439	445	PF00018	0.490
LIG_SH3_4	192	199	PF00018	0.748
LIG_SUMO_SIM_anti_2	91	97	PF11976	0.463
LIG_SUMO_SIM_par_1	429	437	PF11976	0.476
LIG_SxIP_EBH_1	252	266	PF03271	0.726
LIG_TRAF2_1	220	223	PF00917	0.768
LIG_TRAF2_1	365	368	PF00917	0.553
MOD_CK1_1	132	138	PF00069	0.533
MOD_CK1_1	166	172	PF00069	0.692
MOD_CK1_1	218	224	PF00069	0.769
MOD_CK1_1	238	244	PF00069	0.778
MOD_CK1_1	324	330	PF00069	0.534
MOD_CK1_1	393	399	PF00069	0.528
MOD_CK1_1	41	47	PF00069	0.449
MOD_CK1_1	414	420	PF00069	0.560
MOD_CK2_1	132	138	PF00069	0.505
MOD_CK2_1	149	155	PF00069	0.538
MOD_CK2_1	216	222	PF00069	0.689
MOD_CK2_1	267	273	PF00069	0.629
MOD_CK2_1	34	40	PF00069	0.648
MOD_Cter_Amidation	487	490	PF01082	0.360
MOD_GlcNHglycan	127	130	PF01048	0.474
MOD_GlcNHglycan	186	189	PF01048	0.665
MOD_GlcNHglycan	222	227	PF01048	0.746
MOD_GlcNHglycan	36	39	PF01048	0.599
MOD_GlcNHglycan	385	389	PF01048	0.279
MOD_GlcNHglycan	458	461	PF01048	0.308
MOD_GlcNHglycan	486	489	PF01048	0.311
MOD_GSK3_1	125	132	PF00069	0.542
MOD_GSK3_1	158	165	PF00069	0.707
MOD_GSK3_1	176	183	PF00069	0.651
MOD_GSK3_1	199	206	PF00069	0.710
MOD_GSK3_1	214	221	PF00069	0.651
MOD_GSK3_1	234	241	PF00069	0.767
MOD_GSK3_1	250	257	PF00069	0.615
MOD_GSK3_1	327	334	PF00069	0.476
MOD_GSK3_1	34	41	PF00069	0.570
MOD_GSK3_1	372	379	PF00069	0.572
MOD_GSK3_1	434	441	PF00069	0.476
MOD_GSK3_1	464	471	PF00069	0.538
MOD_GSK3_1	480	487	PF00069	0.534
MOD_GSK3_1	504	511	PF00069	0.471
MOD_GSK3_1	84	91	PF00069	0.444
MOD_N-GLC_1	115	120	PF02516	0.372
MOD_N-GLC_1	511	516	PF02516	0.337
MOD_N-GLC_1	527	532	PF02516	0.473
MOD_N-GLC_2	171	173	PF02516	0.647
MOD_NEK2_1	115	120	PF00069	0.380
MOD_NEK2_1	214	219	PF00069	0.719
MOD_NEK2_1	248	253	PF00069	0.728
MOD_NEK2_1	331	336	PF00069	0.508
MOD_NEK2_1	411	416	PF00069	0.524
MOD_NEK2_1	432	437	PF00069	0.476
MOD_NEK2_1	480	485	PF00069	0.560
MOD_NEK2_1	68	73	PF00069	0.568
MOD_NEK2_1	88	93	PF00069	0.435
MOD_NEK2_2	333	338	PF00069	0.556
MOD_NEK2_2	434	439	PF00069	0.507
MOD_PIKK_1	199	205	PF00454	0.758
MOD_PIKK_1	363	369	PF00454	0.507
MOD_PIKK_1	516	522	PF00454	0.431
MOD_PK_1	120	126	PF00069	0.461
MOD_PK_1	208	214	PF00069	0.746
MOD_PKA_1	196	202	PF00069	0.778
MOD_PKA_1	316	322	PF00069	0.560
MOD_PKA_1	489	495	PF00069	0.560
MOD_PKA_2	125	131	PF00069	0.504
MOD_PKA_2	196	202	PF00069	0.701
MOD_PKA_2	315	321	PF00069	0.559
MOD_PKA_2	489	495	PF00069	0.560
MOD_Plk_1	384	390	PF00069	0.470
MOD_Plk_2-3	149	155	PF00069	0.635
MOD_Plk_2-3	308	314	PF00069	0.553
MOD_Plk_4	108	114	PF00069	0.494
MOD_Plk_4	120	126	PF00069	0.391
MOD_Plk_4	142	148	PF00069	0.611
MOD_Plk_4	196	202	PF00069	0.727
MOD_Plk_4	250	256	PF00069	0.655
MOD_Plk_4	324	330	PF00069	0.507
MOD_Plk_4	53	59	PF00069	0.448
MOD_ProDKin_1	158	164	PF00069	0.710
MOD_ProDKin_1	180	186	PF00069	0.673
MOD_ProDKin_1	438	444	PF00069	0.490
MOD_ProDKin_1	468	474	PF00069	0.495
MOD_ProDKin_1	475	481	PF00069	0.482
MOD_SUMO_for_1	303	306	PF00179	0.556
MOD_SUMO_rev_2	136	146	PF00179	0.587
TRG_DiLeu_BaEn_1	8	13	PF01217	0.483
TRG_DiLeu_BaLyEn_6	243	248	PF01217	0.738
TRG_DiLeu_BaLyEn_6	89	94	PF01217	0.430
TRG_ENDOCYTIC_2	389	392	PF00928	0.556
TRG_ER_diArg_1	119	121	PF00400	0.376
TRG_ER_diArg_1	337	339	PF00400	0.556
TRG_ER_diArg_1	489	491	PF00400	0.572
TRG_NES_CRM1_1	49	62	PF08389	0.524
TRG_Pf-PMV_PEXEL_1	230	234	PF00026	0.798
TRG_Pf-PMV_PEXEL_1	339	343	PF00026	0.356

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P3U6	Leptomonas seymouri	72%	100%
A0A0S4JL98	Bodo saltans	38%	89%
A0A1X0NQN7	Trypanosomatidae	48%	100%
A0A3S7WRA1	Leishmania donovani	98%	100%
A0A422NCU2	Trypanosoma rangeli	45%	100%
A4HUK8	Leishmania infantum	98%	100%
E9ANA5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	97%	100%
V5BTT5	Trypanosoma cruzi	46%	100%