Putative DEAD-boc ATP-dependent (RNA) helicase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

DEAD-boc ATP-dependent (RNA) helicase, putative

Species:

Leishmania major

UniProt:

Q4QH61_LEIMA

TriTrypDb:

LmjF.11.0190 , LMJLV39_110006900 , LMJSD75_110006800

Length:

609

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005681	spliceosomal complex	3	2
GO:0032991	protein-containing complex	1	2
GO:0071013	catalytic step 2 spliceosome	3	2
GO:0140513	nuclear protein-containing complex	2	2
GO:1902494	catalytic complex	2	2
GO:1990904	ribonucleoprotein complex	2	2
GO:0016020	membrane	2	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

Q4QH61

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QH61

Function

Biological processes
Term	Name	Level	Count
GO:0000375	RNA splicing, via transesterification reactions	8	2
GO:0000377	RNA splicing, via transesterification reactions with bulged adenosine as nucleophile	9	2
GO:0000398	mRNA splicing, via spliceosome	8	2
GO:0006139	nucleobase-containing compound metabolic process	3	2
GO:0006396	RNA processing	6	2
GO:0006397	mRNA processing	7	2
GO:0006725	cellular aromatic compound metabolic process	3	2
GO:0006807	nitrogen compound metabolic process	2	2
GO:0008152	metabolic process	1	2
GO:0008380	RNA splicing	7	2
GO:0009987	cellular process	1	2
GO:0016070	RNA metabolic process	5	2
GO:0016071	mRNA metabolic process	6	2
GO:0034641	cellular nitrogen compound metabolic process	3	2
GO:0043170	macromolecule metabolic process	3	2
GO:0044237	cellular metabolic process	2	2
GO:0044238	primary metabolic process	2	2
GO:0046483	heterocycle metabolic process	3	2
GO:0071704	organic substance metabolic process	2	2
GO:0090304	nucleic acid metabolic process	4	2
GO:1901360	organic cyclic compound metabolic process	3	2

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	12
GO:0003676	nucleic acid binding	3	12
GO:0003723	RNA binding	4	2
GO:0003824	catalytic activity	1	12
GO:0004386	helicase activity	2	12
GO:0005488	binding	1	12
GO:0005524	ATP binding	5	12
GO:0017076	purine nucleotide binding	4	12
GO:0030554	adenyl nucleotide binding	5	12
GO:0032553	ribonucleotide binding	3	12
GO:0032555	purine ribonucleotide binding	4	12
GO:0032559	adenyl ribonucleotide binding	5	12
GO:0035639	purine ribonucleoside triphosphate binding	4	12
GO:0036094	small molecule binding	2	12
GO:0043167	ion binding	2	12
GO:0043168	anion binding	3	12
GO:0097159	organic cyclic compound binding	2	12
GO:0097367	carbohydrate derivative binding	2	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	12
GO:0140657	ATP-dependent activity	1	12
GO:1901265	nucleoside phosphate binding	3	12
GO:1901363	heterocyclic compound binding	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	91	95	PF00656	0.616
CLV_NRD_NRD_1	255	257	PF00675	0.254
CLV_NRD_NRD_1	294	296	PF00675	0.310
CLV_NRD_NRD_1	401	403	PF00675	0.543
CLV_NRD_NRD_1	443	445	PF00675	0.730
CLV_NRD_NRD_1	6	8	PF00675	0.681
CLV_PCSK_FUR_1	292	296	PF00082	0.315
CLV_PCSK_KEX2_1	123	125	PF00082	0.498
CLV_PCSK_KEX2_1	255	257	PF00082	0.254
CLV_PCSK_KEX2_1	294	296	PF00082	0.306
CLV_PCSK_KEX2_1	401	403	PF00082	0.529
CLV_PCSK_KEX2_1	443	445	PF00082	0.776
CLV_PCSK_KEX2_1	6	8	PF00082	0.653
CLV_PCSK_PC1ET2_1	123	125	PF00082	0.554
CLV_PCSK_SKI1_1	128	132	PF00082	0.420
CLV_PCSK_SKI1_1	357	361	PF00082	0.487
CLV_PCSK_SKI1_1	7	11	PF00082	0.617
CLV_Separin_Metazoa	241	245	PF03568	0.392
DEG_APCC_DBOX_1	356	364	PF00400	0.422
DEG_APCC_DBOX_1	6	14	PF00400	0.677
DEG_Nend_UBRbox_3	1	3	PF02207	0.697
DEG_SPOP_SBC_1	407	411	PF00917	0.505
DOC_ANK_TNKS_1	400	407	PF00023	0.568
DOC_ANK_TNKS_1	539	546	PF00023	0.591
DOC_CKS1_1	233	238	PF01111	0.465
DOC_CYCLIN_RxL_1	351	362	PF00134	0.477
DOC_CYCLIN_yClb5_NLxxxL_5	281	290	PF00134	0.495
DOC_CYCLIN_yCln2_LP_2	114	120	PF00134	0.517
DOC_MAPK_gen_1	253	263	PF00069	0.445
DOC_MAPK_MEF2A_6	157	165	PF00069	0.540
DOC_MAPK_MEF2A_6	357	365	PF00069	0.385
DOC_PP1_RVXF_1	372	379	PF00149	0.385
DOC_PP2B_LxvP_1	114	117	PF13499	0.523
DOC_PP2B_LxvP_1	550	553	PF13499	0.412
DOC_PP2B_LxvP_1	566	569	PF13499	0.391
DOC_USP7_MATH_1	211	215	PF00917	0.490
DOC_USP7_MATH_1	407	411	PF00917	0.505
DOC_USP7_MATH_1	524	528	PF00917	0.524
DOC_USP7_MATH_1	569	573	PF00917	0.527
DOC_USP7_UBL2_3	253	257	PF12436	0.448
DOC_WW_Pin1_4	105	110	PF00397	0.477
DOC_WW_Pin1_4	232	237	PF00397	0.465
DOC_WW_Pin1_4	243	248	PF00397	0.465
LIG_14-3-3_CanoR_1	128	137	PF00244	0.322
LIG_14-3-3_CanoR_1	177	182	PF00244	0.454
LIG_14-3-3_CanoR_1	328	334	PF00244	0.475
LIG_14-3-3_CanoR_1	574	582	PF00244	0.549
LIG_APCC_ABBA_1	375	380	PF00400	0.446
LIG_APCC_ABBAyCdc20_2	374	380	PF00400	0.451
LIG_deltaCOP1_diTrp_1	194	200	PF00928	0.454
LIG_FHA_1	178	184	PF00498	0.456
LIG_FHA_1	202	208	PF00498	0.454
LIG_FHA_1	324	330	PF00498	0.480
LIG_FHA_1	515	521	PF00498	0.454
LIG_FHA_1	72	78	PF00498	0.615
LIG_FHA_2	319	325	PF00498	0.589
LIG_FHA_2	377	383	PF00498	0.424
LIG_FHA_2	390	396	PF00498	0.456
LIG_FHA_2	89	95	PF00498	0.617
LIG_LIR_LC3C_4	495	500	PF02991	0.540
LIG_LIR_Nem_3	341	347	PF02991	0.371
LIG_LIR_Nem_3	47	53	PF02991	0.560
LIG_NBox_RRM_1	159	169	PF00076	0.454
LIG_PCNA_yPIPBox_3	168	182	PF02747	0.454
LIG_Pex14_1	196	200	PF04695	0.454
LIG_SH2_SRC	467	470	PF00017	0.694
LIG_SH2_STAP1	193	197	PF00017	0.454
LIG_SH2_STAT3	103	106	PF00017	0.553
LIG_SH2_STAT3	276	279	PF00017	0.540
LIG_SH2_STAT3	31	34	PF00017	0.589
LIG_SH2_STAT5	12	15	PF00017	0.580
LIG_SH2_STAT5	31	34	PF00017	0.589
LIG_SH2_STAT5	345	348	PF00017	0.355
LIG_SH2_STAT5	483	486	PF00017	0.529
LIG_SH2_STAT5	50	53	PF00017	0.562
LIG_SH3_3	179	185	PF00018	0.540
LIG_SH3_3	230	236	PF00018	0.465
LIG_SH3_3	433	439	PF00018	0.788
LIG_SH3_3	546	552	PF00018	0.417
LIG_SUMO_SIM_anti_2	180	188	PF11976	0.465
LIG_SUMO_SIM_anti_2	546	551	PF11976	0.469
LIG_SUMO_SIM_par_1	180	188	PF11976	0.463
LIG_TRAF2_1	185	188	PF00917	0.446
LIG_WRC_WIRS_1	502	507	PF05994	0.415
MOD_CDK_SPxK_1	232	238	PF00069	0.322
MOD_CK1_1	424	430	PF00069	0.760
MOD_CK1_1	488	494	PF00069	0.408
MOD_CK1_1	504	510	PF00069	0.226
MOD_CK1_1	573	579	PF00069	0.474
MOD_CK1_1	61	67	PF00069	0.696
MOD_CK1_1	88	94	PF00069	0.694
MOD_CK2_1	329	335	PF00069	0.480
MOD_CK2_1	376	382	PF00069	0.427
MOD_CK2_1	389	395	PF00069	0.413
MOD_CK2_1	561	567	PF00069	0.536
MOD_CK2_1	573	579	PF00069	0.417
MOD_GlcNHglycan	109	112	PF01048	0.331
MOD_GlcNHglycan	13	16	PF01048	0.658
MOD_GlcNHglycan	22	25	PF01048	0.593
MOD_GlcNHglycan	281	284	PF01048	0.332
MOD_GlcNHglycan	305	308	PF01048	0.306
MOD_GlcNHglycan	392	395	PF01048	0.594
MOD_GlcNHglycan	426	429	PF01048	0.749
MOD_GlcNHglycan	457	460	PF01048	0.735
MOD_GlcNHglycan	462	465	PF01048	0.697
MOD_GlcNHglycan	526	529	PF01048	0.408
MOD_GlcNHglycan	554	557	PF01048	0.442
MOD_GlcNHglycan	575	578	PF01048	0.472
MOD_GlcNHglycan	583	586	PF01048	0.517
MOD_GlcNHglycan	591	594	PF01048	0.483
MOD_GlcNHglycan	599	602	PF01048	0.707
MOD_GlcNHglycan	63	66	PF01048	0.751
MOD_GSK3_1	148	155	PF00069	0.427
MOD_GSK3_1	323	330	PF00069	0.518
MOD_GSK3_1	334	341	PF00069	0.386
MOD_GSK3_1	386	393	PF00069	0.479
MOD_GSK3_1	501	508	PF00069	0.334
MOD_GSK3_1	569	576	PF00069	0.582
MOD_GSK3_1	595	602	PF00069	0.620
MOD_GSK3_1	84	91	PF00069	0.708
MOD_N-GLC_1	485	490	PF02516	0.365
MOD_N-GLC_1	505	510	PF02516	0.123
MOD_NEK2_1	27	32	PF00069	0.599
MOD_NEK2_1	329	334	PF00069	0.492
MOD_NEK2_1	347	352	PF00069	0.309
MOD_NEK2_1	359	364	PF00069	0.465
MOD_NEK2_1	365	370	PF00069	0.494
MOD_NEK2_1	408	413	PF00069	0.447
MOD_NEK2_1	505	510	PF00069	0.455
MOD_NEK2_1	570	575	PF00069	0.531
MOD_NEK2_1	84	89	PF00069	0.630
MOD_PIKK_1	202	208	PF00454	0.326
MOD_PIKK_1	365	371	PF00454	0.517
MOD_PIKK_1	44	50	PF00454	0.509
MOD_PIKK_1	448	454	PF00454	0.673
MOD_PIKK_1	66	72	PF00454	0.621
MOD_PIKK_1	85	91	PF00454	0.559
MOD_PKA_2	2	8	PF00069	0.637
MOD_PKA_2	327	333	PF00069	0.466
MOD_PKA_2	460	466	PF00069	0.730
MOD_PKA_2	573	579	PF00069	0.537
MOD_PKA_2	587	593	PF00069	0.679
MOD_PKB_1	126	134	PF00069	0.455
MOD_Plk_1	323	329	PF00069	0.519
MOD_Plk_1	334	340	PF00069	0.392
MOD_Plk_1	485	491	PF00069	0.308
MOD_Plk_1	58	64	PF00069	0.742
MOD_Plk_4	177	183	PF00069	0.306
MOD_Plk_4	245	251	PF00069	0.306
MOD_Plk_4	27	33	PF00069	0.559
MOD_Plk_4	329	335	PF00069	0.514
MOD_Plk_4	386	392	PF00069	0.528
MOD_ProDKin_1	105	111	PF00069	0.477
MOD_ProDKin_1	232	238	PF00069	0.322
MOD_ProDKin_1	243	249	PF00069	0.322
MOD_SUMO_rev_2	424	433	PF00179	0.650
MOD_SUMO_rev_2	489	499	PF00179	0.427
TRG_DiLeu_BaEn_1	356	361	PF01217	0.505
TRG_DiLeu_BaEn_1	386	391	PF01217	0.484
TRG_DiLeu_BaEn_1	544	549	PF01217	0.551
TRG_DiLeu_BaLyEn_6	136	141	PF01217	0.391
TRG_ENDOCYTIC_2	467	470	PF00928	0.694
TRG_ENDOCYTIC_2	50	53	PF00928	0.612
TRG_ER_diArg_1	124	127	PF00400	0.490
TRG_ER_diArg_1	254	256	PF00400	0.305
TRG_ER_diArg_1	291	294	PF00400	0.275
TRG_ER_diArg_1	400	402	PF00400	0.527
TRG_ER_diArg_1	443	445	PF00400	0.734
TRG_NES_CRM1_1	172	188	PF08389	0.322
TRG_NES_CRM1_1	252	267	PF08389	0.306
TRG_Pf-PMV_PEXEL_1	168	172	PF00026	0.322

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IJV4	Leptomonas seymouri	74%	100%
A0A0S4IX79	Bodo saltans	24%	84%
A0A0S4JYL8	Bodo saltans	49%	92%
A0A1X0NV90	Trypanosomatidae	60%	100%
A0A3Q8ICA8	Leishmania donovani	94%	100%
A0A3R7MZZ8	Trypanosoma rangeli	59%	100%
A4H9T9	Leishmania braziliensis	87%	93%
A4HUT1	Leishmania infantum	94%	100%
A5DPU0	Meyerozyma guilliermondii (strain ATCC 6260 / CBS 566 / DSM 6381 / JCM 1539 / NBRC 10279 / NRRL Y-324)	25%	81%
D0A7N1	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	58%	100%
D0AAP5	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	23%	100%
E9ANG5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	93%	100%
Q09719	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	25%	72%
Q2UFL0	Aspergillus oryzae (strain ATCC 42149 / RIB 40)	23%	100%
Q9Y7T7	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	22%	92%
V5BG99	Trypanosoma cruzi	23%	74%
V5C100	Trypanosoma cruzi	60%	99%