DUF676 domain-containing protein

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Alpha/beta hydrolase family, putative

Species:

Leishmania major

UniProt:

Q4QGZ5_LEIMA

TriTrypDb:

LmjF.11.0840 , LMJLV39_110015000 , LMJSD75_110015600

Length:

377

Annotations

LeishMANIAdb annotations

Posesses a conserved AB hydrolase domain. Due to the distribution of hydrophilic / hydrophobic amino acids, it likely only has a perimembrane helix, not a full TM one.

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 9

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 25
NetGPI	no	yes: 0, no: 25

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	6
GO:0110165	cellular anatomical entity	1	6

Expansion

Sequence features

Q4QGZ5

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QGZ5

Function

Biological processes
Term	Name	Level	Count
GO:0006629	lipid metabolic process	3	4
GO:0008152	metabolic process	1	4
GO:0009987	cellular process	1	4
GO:0044237	cellular metabolic process	2	4
GO:0044238	primary metabolic process	2	4
GO:0044255	cellular lipid metabolic process	3	4
GO:0071704	organic substance metabolic process	2	4

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	6
GO:0016298	lipase activity	4	4
GO:0016787	hydrolase activity	2	6
GO:0016788	hydrolase activity, acting on ester bonds	3	4
GO:0034338	short-chain carboxylesterase activity	5	4
GO:0047372	acylglycerol lipase activity	5	4
GO:0052689	carboxylic ester hydrolase activity	4	4

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	48	52	PF00656	0.402
CLV_NRD_NRD_1	138	140	PF00675	0.577
CLV_NRD_NRD_1	20	22	PF00675	0.455
CLV_NRD_NRD_1	283	285	PF00675	0.413
CLV_NRD_NRD_1	79	81	PF00675	0.411
CLV_PCSK_KEX2_1	138	140	PF00082	0.612
CLV_PCSK_KEX2_1	20	22	PF00082	0.455
CLV_PCSK_KEX2_1	217	219	PF00082	0.440
CLV_PCSK_KEX2_1	250	252	PF00082	0.341
CLV_PCSK_KEX2_1	283	285	PF00082	0.413
CLV_PCSK_KEX2_1	79	81	PF00082	0.411
CLV_PCSK_PC1ET2_1	217	219	PF00082	0.461
CLV_PCSK_PC1ET2_1	250	252	PF00082	0.341
CLV_PCSK_SKI1_1	165	169	PF00082	0.361
CLV_PCSK_SKI1_1	203	207	PF00082	0.351
CLV_PCSK_SKI1_1	227	231	PF00082	0.461
CLV_PCSK_SKI1_1	238	242	PF00082	0.416
CLV_PCSK_SKI1_1	83	87	PF00082	0.399
DEG_APCC_DBOX_1	202	210	PF00400	0.553
DOC_CYCLIN_RxL_1	200	207	PF00134	0.278
DOC_CYCLIN_RxL_1	308	317	PF00134	0.308
DOC_MAPK_DCC_7	150	158	PF00069	0.538
DOC_MAPK_gen_1	58	66	PF00069	0.395
DOC_MAPK_MEF2A_6	150	158	PF00069	0.440
DOC_MAPK_MEF2A_6	289	297	PF00069	0.353
DOC_MAPK_MEF2A_6	58	66	PF00069	0.394
DOC_PP1_RVXF_1	18	25	PF00149	0.304
DOC_PP2B_PxIxI_1	153	159	PF00149	0.453
DOC_PP4_FxxP_1	11	14	PF00568	0.582
DOC_USP7_MATH_1	142	146	PF00917	0.563
DOC_USP7_MATH_1	278	282	PF00917	0.247
DOC_WW_Pin1_4	138	143	PF00397	0.632
DOC_WW_Pin1_4	188	193	PF00397	0.363
DOC_WW_Pin1_4	243	248	PF00397	0.393
LIG_14-3-3_CanoR_1	13	18	PF00244	0.469
LIG_14-3-3_CanoR_1	251	256	PF00244	0.456
LIG_Actin_WH2_1	107	122	PF00022	0.262
LIG_BIR_II_1	1	5	PF00653	0.422
LIG_BRCT_BRCA1_1	111	115	PF00533	0.437
LIG_BRCT_BRCA1_1	72	76	PF00533	0.448
LIG_BRCT_BRCA1_2	111	117	PF00533	0.257
LIG_Clathr_ClatBox_1	323	327	PF01394	0.453
LIG_Clathr_ClatBox_2	339	344	PF01394	0.337
LIG_CSL_BTD_1	37	40	PF09270	0.452
LIG_FHA_1	1	7	PF00498	0.572
LIG_FHA_1	166	172	PF00498	0.328
LIG_FHA_1	90	96	PF00498	0.371
LIG_FHA_2	101	107	PF00498	0.468
LIG_FHA_2	252	258	PF00498	0.383
LIG_FHA_2	31	37	PF00498	0.353
LIG_FHA_2	39	45	PF00498	0.463
LIG_FHA_2	46	52	PF00498	0.622
LIG_LIR_Apic_2	300	306	PF02991	0.260
LIG_LIR_Apic_2	36	40	PF02991	0.453
LIG_LIR_Gen_1	181	189	PF02991	0.459
LIG_LIR_Nem_3	181	186	PF02991	0.466
LIG_LIR_Nem_3	213	219	PF02991	0.310
LIG_LIR_Nem_3	257	261	PF02991	0.413
LIG_LIR_Nem_3	280	285	PF02991	0.354
LIG_NRBOX	201	207	PF00104	0.278
LIG_Pex14_1	24	28	PF04695	0.489
LIG_Pex14_2	111	115	PF04695	0.410
LIG_SH2_CRK	216	220	PF00017	0.439
LIG_SH2_CRK	303	307	PF00017	0.499
LIG_SH2_STAT5	170	173	PF00017	0.374
LIG_SH2_STAT5	187	190	PF00017	0.351
LIG_SH2_STAT5	276	279	PF00017	0.360
LIG_SH2_STAT5	325	328	PF00017	0.376
LIG_SH2_STAT5	77	80	PF00017	0.356
LIG_SH3_3	264	270	PF00018	0.365
LIG_SH3_3	302	308	PF00018	0.432
LIG_SH3_3	323	329	PF00018	0.366
LIG_SH3_3	61	67	PF00018	0.366
LIG_SH3_CIN85_PxpxPR_1	306	311	PF14604	0.301
LIG_TRAF2_2	142	147	PF00917	0.429
LIG_UBA3_1	110	117	PF00899	0.435
LIG_UBA3_1	359	364	PF00899	0.425
LIG_UBA3_1	65	74	PF00899	0.400
LIG_ULM_U2AF65_1	20	25	PF00076	0.291
MOD_CDK_SPxxK_3	243	250	PF00069	0.322
MOD_CK1_1	342	348	PF00069	0.504
MOD_CK1_1	365	371	PF00069	0.513
MOD_CK2_1	100	106	PF00069	0.467
MOD_CK2_1	38	44	PF00069	0.365
MOD_CK2_1	69	75	PF00069	0.444
MOD_CMANNOS	341	344	PF00535	0.426
MOD_GlcNHglycan	111	114	PF01048	0.366
MOD_GlcNHglycan	180	183	PF01048	0.443
MOD_GlcNHglycan	364	367	PF01048	0.468
MOD_GSK3_1	138	145	PF00069	0.515
MOD_GSK3_1	165	172	PF00069	0.345
MOD_GSK3_1	89	96	PF00069	0.414
MOD_N-GLC_1	193	198	PF02516	0.399
MOD_N-GLC_1	30	35	PF02516	0.218
MOD_NEK2_1	204	209	PF00069	0.495
MOD_NEK2_1	210	215	PF00069	0.484
MOD_NEK2_1	285	290	PF00069	0.371
MOD_NEK2_1	69	74	PF00069	0.384
MOD_NEK2_2	211	216	PF00069	0.497
MOD_NEK2_2	278	283	PF00069	0.451
MOD_PIKK_1	342	348	PF00454	0.471
MOD_PIKK_1	365	371	PF00454	0.483
MOD_PKA_2	12	18	PF00069	0.502
MOD_Plk_1	30	36	PF00069	0.353
MOD_Plk_4	211	217	PF00069	0.439
MOD_ProDKin_1	138	144	PF00069	0.631
MOD_ProDKin_1	188	194	PF00069	0.366
MOD_ProDKin_1	243	249	PF00069	0.385
MOD_SUMO_rev_2	235	241	PF00179	0.274
MOD_SUMO_rev_2	367	375	PF00179	0.561
TRG_DiLeu_BaEn_1	350	355	PF01217	0.394
TRG_ENDOCYTIC_2	216	219	PF00928	0.435
TRG_ENDOCYTIC_2	325	328	PF00928	0.321
TRG_ER_diArg_1	137	139	PF00400	0.372
TRG_ER_diArg_1	20	23	PF00400	0.445
TRG_ER_diArg_1	282	284	PF00400	0.464
TRG_ER_diArg_1	57	60	PF00400	0.453
TRG_ER_diArg_1	78	80	PF00400	0.412
TRG_NES_CRM1_1	231	245	PF08389	0.293
TRG_NLS_Bipartite_1	238	254	PF00514	0.267
TRG_NLS_MonoExtN_4	247	254	PF00514	0.415
TRG_Pf-PMV_PEXEL_1	120	124	PF00026	0.448

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P5L7	Leptomonas seymouri	25%	83%
A0A0N1HZ60	Leptomonas seymouri	32%	81%
A0A0N1I523	Leptomonas seymouri	28%	85%
A0A0N1I5Q2	Leptomonas seymouri	64%	74%
A0A0N1I7U8	Leptomonas seymouri	30%	88%
A0A0S4JMS7	Bodo saltans	33%	87%
A0A0S4JTS9	Bodo saltans	31%	88%
A0A1X0NEW5	Trypanosomatidae	31%	90%
A0A1X0NSK7	Trypanosomatidae	31%	90%
A0A1X0NUZ6	Trypanosomatidae	30%	90%
A0A3S5H6J1	Leishmania donovani	35%	80%
A0A3S7X2V6	Leishmania donovani	31%	89%
A0A422NB49	Trypanosoma rangeli	32%	96%
A0A422NQC5	Trypanosoma rangeli	38%	86%
A4H6L0	Leishmania braziliensis	77%	77%
A4HHU6	Leishmania braziliensis	30%	88%
A4HUZ4	Leishmania infantum	35%	80%
A4I4Z6	Leishmania infantum	31%	89%
C9ZPB9	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	30%	88%
E9ANM8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	79%	91%
E9ANM9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	34%	80%
E9B0C6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	31%	89%
P38295	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	28%	84%
Q02891	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	24%	83%
Q03649	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	25%	84%
Q0VC00	Bos taurus	24%	92%
Q18610	Caenorhabditis elegans	23%	100%
Q3T0A0	Bos taurus	25%	93%
Q40863	Picea glauca	27%	82%
Q4Q7V8	Leishmania major	30%	100%
Q4QGZ4	Leishmania major	36%	100%
Q54H38	Dictyostelium discoideum	24%	95%
Q5RK23	Rattus norvegicus	23%	92%
Q8WU67	Homo sapiens	24%	92%
Q91ZH7	Mus musculus	25%	92%
Q96SE0	Homo sapiens	25%	93%
Q9QZC8	Mus musculus	24%	92%
V5AZF4	Trypanosoma cruzi	34%	91%
V5BBC2	Trypanosoma cruzi	43%	88%