Putative eukaryotic release factor 3

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

eukaryotic release factor 3, putative

Species:

Leishmania major

UniProt:

Q4QGW5_LEIMA

TriTrypDb:

LmjF.11.1170 , LMJLV39_110019100 , LMJSD75_110019500

Length:

763

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 1
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 2
Silverman et al.	yes	yes: 2
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 6

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	2
GO:0018444	translation release factor complex	2	2
GO:0032991	protein-containing complex	1	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

Q4QGW5

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QGW5

Function

Biological processes
Term	Name	Level	Count
GO:0006412	translation	4	2
GO:0006518	peptide metabolic process	4	2
GO:0006807	nitrogen compound metabolic process	2	2
GO:0008152	metabolic process	1	2
GO:0009058	biosynthetic process	2	2
GO:0009059	macromolecule biosynthetic process	4	2
GO:0009987	cellular process	1	2
GO:0019538	protein metabolic process	3	2
GO:0034641	cellular nitrogen compound metabolic process	3	2
GO:0034645	obsolete cellular macromolecule biosynthetic process	4	2
GO:0043043	peptide biosynthetic process	5	2
GO:0043170	macromolecule metabolic process	3	2
GO:0043603	amide metabolic process	3	2
GO:0043604	amide biosynthetic process	4	2
GO:0044237	cellular metabolic process	2	2
GO:0044238	primary metabolic process	2	2
GO:0044249	cellular biosynthetic process	3	2
GO:0044260	obsolete cellular macromolecule metabolic process	3	2
GO:0044271	cellular nitrogen compound biosynthetic process	4	2
GO:0071704	organic substance metabolic process	2	2
GO:1901564	organonitrogen compound metabolic process	3	2
GO:1901566	organonitrogen compound biosynthetic process	4	2
GO:1901576	organic substance biosynthetic process	3	2

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	11
GO:0003676	nucleic acid binding	3	3
GO:0003747	translation release factor activity	5	2
GO:0003824	catalytic activity	1	11
GO:0003924	GTPase activity	7	11
GO:0005488	binding	1	11
GO:0005525	GTP binding	5	11
GO:0008079	translation termination factor activity	4	2
GO:0008135	translation factor activity, RNA binding	3	3
GO:0016462	pyrophosphatase activity	5	11
GO:0016787	hydrolase activity	2	11
GO:0016817	hydrolase activity, acting on acid anhydrides	3	11
GO:0016818	hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	4	11
GO:0017076	purine nucleotide binding	4	11
GO:0017111	ribonucleoside triphosphate phosphatase activity	6	11
GO:0019001	guanyl nucleotide binding	5	11
GO:0032553	ribonucleotide binding	3	11
GO:0032555	purine ribonucleotide binding	4	11
GO:0032561	guanyl ribonucleotide binding	5	11
GO:0035639	purine ribonucleoside triphosphate binding	4	11
GO:0036094	small molecule binding	2	11
GO:0043167	ion binding	2	11
GO:0043168	anion binding	3	11
GO:0045182	translation regulator activity	1	3
GO:0090079	translation regulator activity, nucleic acid binding	2	3
GO:0097159	organic cyclic compound binding	2	11
GO:0097367	carbohydrate derivative binding	2	11
GO:1901265	nucleoside phosphate binding	3	11
GO:1901363	heterocyclic compound binding	2	11
GO:0003746	translation elongation factor activity	4	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	178	182	PF00656	0.778
CLV_C14_Caspase3-7	407	411	PF00656	0.481
CLV_NRD_NRD_1	300	302	PF00675	0.615
CLV_NRD_NRD_1	362	364	PF00675	0.295
CLV_NRD_NRD_1	404	406	PF00675	0.281
CLV_NRD_NRD_1	689	691	PF00675	0.320
CLV_PCSK_KEX2_1	300	302	PF00082	0.655
CLV_PCSK_KEX2_1	320	322	PF00082	0.356
CLV_PCSK_KEX2_1	362	364	PF00082	0.281
CLV_PCSK_KEX2_1	403	405	PF00082	0.282
CLV_PCSK_PC1ET2_1	320	322	PF00082	0.653
CLV_PCSK_PC1ET2_1	403	405	PF00082	0.292
CLV_PCSK_SKI1_1	388	392	PF00082	0.292
CLV_PCSK_SKI1_1	404	408	PF00082	0.245
CLV_PCSK_SKI1_1	492	496	PF00082	0.281
CLV_PCSK_SKI1_1	667	671	PF00082	0.295
CLV_PCSK_SKI1_1	732	736	PF00082	0.281
DEG_SPOP_SBC_1	269	273	PF00917	0.725
DOC_ANK_TNKS_1	739	746	PF00023	0.585
DOC_CKS1_1	244	249	PF01111	0.726
DOC_CYCLIN_yCln2_LP_2	715	721	PF00134	0.561
DOC_MAPK_gen_1	199	208	PF00069	0.669
DOC_MAPK_gen_1	210	216	PF00069	0.671
DOC_MAPK_gen_1	320	331	PF00069	0.346
DOC_MAPK_gen_1	504	513	PF00069	0.512
DOC_MAPK_gen_1	534	541	PF00069	0.549
DOC_MAPK_MEF2A_6	740	748	PF00069	0.505
DOC_PP1_RVXF_1	504	511	PF00149	0.495
DOC_PP1_RVXF_1	642	649	PF00149	0.407
DOC_PP2B_LxvP_1	409	412	PF13499	0.481
DOC_PP2B_LxvP_1	545	548	PF13499	0.490
DOC_PP4_FxxP_1	557	560	PF00568	0.473
DOC_USP7_MATH_1	112	116	PF00917	0.537
DOC_USP7_MATH_1	12	16	PF00917	0.599
DOC_USP7_MATH_1	168	172	PF00917	0.738
DOC_USP7_MATH_1	175	179	PF00917	0.689
DOC_USP7_MATH_1	228	232	PF00917	0.813
DOC_USP7_MATH_1	242	246	PF00917	0.806
DOC_USP7_MATH_1	337	341	PF00917	0.481
DOC_USP7_MATH_1	64	68	PF00917	0.774
DOC_USP7_MATH_2	221	227	PF00917	0.720
DOC_USP7_UBL2_3	290	294	PF12436	0.699
DOC_USP7_UBL2_3	316	320	PF12436	0.639
DOC_USP7_UBL2_3	566	570	PF12436	0.520
DOC_USP7_UBL2_3	683	687	PF12436	0.481
DOC_USP7_UBL2_3	725	729	PF12436	0.480
DOC_WW_Pin1_4	15	20	PF00397	0.731
DOC_WW_Pin1_4	226	231	PF00397	0.759
DOC_WW_Pin1_4	243	248	PF00397	0.791
DOC_WW_Pin1_4	253	258	PF00397	0.805
DOC_WW_Pin1_4	293	298	PF00397	0.632
DOC_WW_Pin1_4	35	40	PF00397	0.670
LIG_14-3-3_CanoR_1	404	409	PF00244	0.511
LIG_14-3-3_CanoR_1	483	491	PF00244	0.414
LIG_14-3-3_CanoR_1	80	84	PF00244	0.578
LIG_APCC_ABBA_1	721	726	PF00400	0.533
LIG_APCC_ABBAyCdc20_2	80	86	PF00400	0.725
LIG_BIR_II_1	1	5	PF00653	0.639
LIG_BIR_III_2	241	245	PF00653	0.699
LIG_BIR_III_4	410	414	PF00653	0.481
LIG_BRCT_BRCA1_1	605	609	PF00533	0.539
LIG_BRCT_BRCA1_2	605	611	PF00533	0.530
LIG_FHA_1	203	209	PF00498	0.681
LIG_FHA_1	459	465	PF00498	0.481
LIG_FHA_1	5	11	PF00498	0.726
LIG_FHA_1	690	696	PF00498	0.481
LIG_FHA_1	739	745	PF00498	0.481
LIG_FHA_2	217	223	PF00498	0.756
LIG_FHA_2	230	236	PF00498	0.831
LIG_FHA_2	265	271	PF00498	0.809
LIG_FHA_2	286	292	PF00498	0.681
LIG_FHA_2	294	300	PF00498	0.555
LIG_FHA_2	405	411	PF00498	0.481
LIG_FHA_2	424	430	PF00498	0.481
LIG_FHA_2	442	448	PF00498	0.481
LIG_FHA_2	535	541	PF00498	0.506
LIG_FHA_2	550	556	PF00498	0.449
LIG_FHA_2	591	597	PF00498	0.463
LIG_FHA_2	605	611	PF00498	0.383
LIG_FXI_DFP_1	724	728	PF00024	0.295
LIG_IRF3_LxIS_1	430	436	PF10401	0.481
LIG_LIR_Apic_2	2	6	PF02991	0.649
LIG_LIR_Apic_2	554	560	PF02991	0.490
LIG_LIR_Apic_2	82	87	PF02991	0.568
LIG_LIR_Gen_1	374	381	PF02991	0.481
LIG_LIR_Gen_1	482	491	PF02991	0.499
LIG_LIR_Gen_1	678	688	PF02991	0.495
LIG_LIR_Gen_1	726	735	PF02991	0.473
LIG_LIR_Nem_3	374	378	PF02991	0.481
LIG_LIR_Nem_3	482	487	PF02991	0.499
LIG_LIR_Nem_3	571	577	PF02991	0.508
LIG_LIR_Nem_3	678	684	PF02991	0.495
LIG_LIR_Nem_3	726	730	PF02991	0.517
LIG_PCNA_APIM_2	730	736	PF02747	0.481
LIG_Pex14_1	373	377	PF04695	0.481
LIG_Pex14_2	327	331	PF04695	0.495
LIG_Pex14_2	442	446	PF04695	0.481
LIG_REV1ctd_RIR_1	646	656	PF16727	0.533
LIG_SH2_CRK	100	104	PF00017	0.812
LIG_SH2_CRK	37	41	PF00017	0.665
LIG_SH2_CRK	375	379	PF00017	0.481
LIG_SH2_GRB2like	24	27	PF00017	0.688
LIG_SH2_GRB2like	63	66	PF00017	0.715
LIG_SH2_NCK_1	37	41	PF00017	0.730
LIG_SH2_SRC	24	27	PF00017	0.696
LIG_SH2_SRC	375	378	PF00017	0.481
LIG_SH2_SRC	84	87	PF00017	0.751
LIG_SH2_STAP1	110	114	PF00017	0.548
LIG_SH2_STAP1	375	379	PF00017	0.481
LIG_SH2_STAP1	484	488	PF00017	0.445
LIG_SH2_STAT3	122	125	PF00017	0.694
LIG_SH2_STAT3	141	144	PF00017	0.337
LIG_SH2_STAT3	28	31	PF00017	0.709
LIG_SH2_STAT3	49	52	PF00017	0.682
LIG_SH2_STAT3	74	77	PF00017	0.705
LIG_SH2_STAT5	17	20	PF00017	0.738
LIG_SH2_STAT5	24	27	PF00017	0.749
LIG_SH2_STAT5	377	380	PF00017	0.481
LIG_SH2_STAT5	398	401	PF00017	0.481
LIG_SH2_STAT5	634	637	PF00017	0.388
LIG_SH3_3	241	247	PF00018	0.783
LIG_SH3_3	717	723	PF00018	0.533
LIG_SUMO_SIM_par_1	431	437	PF11976	0.481
LIG_SUMO_SIM_par_1	652	657	PF11976	0.481
LIG_SUMO_SIM_par_1	716	722	PF11976	0.561
LIG_SxIP_EBH_1	632	644	PF03271	0.445
LIG_TRAF2_1	220	223	PF00917	0.757
LIG_TRAF2_1	257	260	PF00917	0.722
LIG_TRAF2_1	296	299	PF00917	0.582
LIG_TRAF2_1	382	385	PF00917	0.512
LIG_TRAF2_1	674	677	PF00917	0.470
LIG_UBA3_1	467	473	PF00899	0.495
LIG_UBA3_1	538	546	PF00899	0.585
LIG_UBA3_1	684	691	PF00899	0.533
MOD_CDK_SPxxK_3	293	300	PF00069	0.722
MOD_CK1_1	15	21	PF00069	0.542
MOD_CK1_1	191	197	PF00069	0.741
MOD_CK1_1	226	232	PF00069	0.800
MOD_CK1_1	340	346	PF00069	0.470
MOD_CK1_1	436	442	PF00069	0.495
MOD_CK1_1	603	609	PF00069	0.520
MOD_CK1_1	638	644	PF00069	0.422
MOD_CK1_1	663	669	PF00069	0.561
MOD_CK2_1	216	222	PF00069	0.755
MOD_CK2_1	229	235	PF00069	0.697
MOD_CK2_1	264	270	PF00069	0.747
MOD_CK2_1	293	299	PF00069	0.587
MOD_CK2_1	379	385	PF00069	0.481
MOD_CK2_1	441	447	PF00069	0.481
MOD_CK2_1	534	540	PF00069	0.491
MOD_CK2_1	547	553	PF00069	0.405
MOD_CK2_1	590	596	PF00069	0.429
MOD_CK2_1	604	610	PF00069	0.422
MOD_CK2_1	671	677	PF00069	0.585
MOD_CK2_1	750	756	PF00069	0.482
MOD_Cter_Amidation	208	211	PF01082	0.694
MOD_GlcNHglycan	110	113	PF01048	0.793
MOD_GlcNHglycan	177	180	PF01048	0.758
MOD_GlcNHglycan	190	193	PF01048	0.718
MOD_GlcNHglycan	262	265	PF01048	0.764
MOD_GlcNHglycan	276	279	PF01048	0.692
MOD_GlcNHglycan	342	345	PF01048	0.281
MOD_GlcNHglycan	578	581	PF01048	0.389
MOD_GlcNHglycan	662	665	PF01048	0.361
MOD_GlcNHglycan	673	676	PF01048	0.361
MOD_GSK3_1	108	115	PF00069	0.547
MOD_GSK3_1	226	233	PF00069	0.722
MOD_GSK3_1	260	267	PF00069	0.807
MOD_GSK3_1	270	277	PF00069	0.715
MOD_GSK3_1	384	391	PF00069	0.512
MOD_GSK3_1	600	607	PF00069	0.430
MOD_GSK3_1	634	641	PF00069	0.417
MOD_GSK3_1	750	757	PF00069	0.499
MOD_N-GLC_1	112	117	PF02516	0.786
MOD_N-GLC_1	12	17	PF02516	0.551
MOD_N-GLC_1	64	69	PF02516	0.712
MOD_NEK2_1	433	438	PF00069	0.481
MOD_NEK2_1	458	463	PF00069	0.481
MOD_NEK2_1	750	755	PF00069	0.469
MOD_NEK2_2	69	74	PF00069	0.568
MOD_PIKK_1	223	229	PF00454	0.727
MOD_PIKK_1	264	270	PF00454	0.816
MOD_PIKK_1	423	429	PF00454	0.481
MOD_PKA_1	300	306	PF00069	0.649
MOD_PKA_1	404	410	PF00069	0.481
MOD_PKA_2	300	306	PF00069	0.540
MOD_PKA_2	391	397	PF00069	0.490
MOD_PKA_2	404	410	PF00069	0.461
MOD_PKA_2	482	488	PF00069	0.434
MOD_PKA_2	689	695	PF00069	0.528
MOD_PKA_2	79	85	PF00069	0.578
MOD_PKB_1	310	318	PF00069	0.708
MOD_Plk_1	112	118	PF00069	0.557
MOD_Plk_1	12	18	PF00069	0.550
MOD_Plk_1	677	683	PF00069	0.495
MOD_Plk_2-3	549	555	PF00069	0.476
MOD_Plk_2-3	600	606	PF00069	0.425
MOD_Plk_4	112	118	PF00069	0.539
MOD_Plk_4	404	410	PF00069	0.490
MOD_Plk_4	417	423	PF00069	0.461
MOD_Plk_4	534	540	PF00069	0.499
MOD_Plk_4	69	75	PF00069	0.565
MOD_Plk_4	79	85	PF00069	0.527
MOD_ProDKin_1	15	21	PF00069	0.731
MOD_ProDKin_1	226	232	PF00069	0.761
MOD_ProDKin_1	243	249	PF00069	0.793
MOD_ProDKin_1	253	259	PF00069	0.806
MOD_ProDKin_1	293	299	PF00069	0.626
MOD_ProDKin_1	35	41	PF00069	0.671
MOD_SUMO_for_1	236	239	PF00179	0.727
MOD_SUMO_for_1	304	307	PF00179	0.589
MOD_SUMO_rev_2	229	238	PF00179	0.783
MOD_SUMO_rev_2	239	245	PF00179	0.793
MOD_SUMO_rev_2	475	480	PF00179	0.559
MOD_SUMO_rev_2	540	548	PF00179	0.589
MOD_SUMO_rev_2	567	571	PF00179	0.519
TRG_DiLeu_BaEn_1	454	459	PF01217	0.481
TRG_ENDOCYTIC_2	375	378	PF00928	0.481
TRG_ENDOCYTIC_2	484	487	PF00928	0.481
TRG_ENDOCYTIC_2	574	577	PF00928	0.402
TRG_ER_diArg_1	361	363	PF00400	0.495
TRG_ER_diArg_1	404	406	PF00400	0.480
TRG_NLS_Bipartite_1	199	214	PF00514	0.778
TRG_NLS_MonoExtN_4	198	203	PF00514	0.649
TRG_Pf-PMV_PEXEL_1	623	627	PF00026	0.484

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PAZ5	Leptomonas seymouri	79%	100%
A0A0S4IX91	Bodo saltans	63%	100%
A0A1X0NV54	Trypanosomatidae	71%	100%
A0A3Q8ICH2	Leishmania donovani	94%	100%
A0A3R7MLL0	Trypanosoma rangeli	71%	100%
A0A3S7X9T0	Leishmania donovani	30%	100%
A4H6P3	Leishmania braziliensis	88%	100%
A4HN87	Leishmania braziliensis	32%	100%
A4HV24	Leishmania infantum	94%	100%
A4IBV5	Leishmania infantum	30%	100%
C9ZYQ4	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	31%	100%
D0A7C5	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	60%	100%
E9AFP2	Leishmania major	31%	100%
E9ANQ6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	97%	99%
E9B6U5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	31%	100%
O74718	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	38%	100%
P05453	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	36%	100%
P23637	Ogataea pini	40%	100%
Q9HGI7	Candida maltosa	38%	100%
Q9HGI8	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)	38%	100%
Q9W074	Drosophila melanogaster	35%	100%