Putative translation initiation factor EIF-2b alpha subunit

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

translation initiation factor EIF-2b alpha subunit, putative

Species:

Leishmania major

UniProt:

Q4QGU3_LEIMA

TriTrypDb:

LmjF.12.0010 , LMJLV39_120005100 , LMJSD75_120005900

Length:

336

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	2
GO:0005851	eukaryotic translation initiation factor 2B complex	2	2
GO:0032991	protein-containing complex	1	2
GO:0110165	cellular anatomical entity	1	3
GO:0016020	membrane	2	1

Expansion

Sequence features

Q4QGU3

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QGU3

Function

Biological processes
Term	Name	Level	Count
GO:0006413	translational initiation	3	2
GO:0008152	metabolic process	1	2
GO:0009987	cellular process	1	2
GO:0044237	cellular metabolic process	2	2
GO:0050790	regulation of catalytic activity	3	2
GO:0065007	biological regulation	1	2
GO:0065009	regulation of molecular function	2	2

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	12
GO:0003743	translation initiation factor activity	4	12
GO:0005488	binding	1	12
GO:0008135	translation factor activity, RNA binding	3	12
GO:0045182	translation regulator activity	1	12
GO:0090079	translation regulator activity, nucleic acid binding	2	12
GO:0097159	organic cyclic compound binding	2	12
GO:1901363	heterocyclic compound binding	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	177	179	PF00675	0.533
CLV_PCSK_KEX2_1	114	116	PF00082	0.548
CLV_PCSK_KEX2_1	334	336	PF00082	0.642
CLV_PCSK_KEX2_1	79	81	PF00082	0.517
CLV_PCSK_PC1ET2_1	114	116	PF00082	0.548
CLV_PCSK_PC1ET2_1	334	336	PF00082	0.661
CLV_PCSK_PC1ET2_1	79	81	PF00082	0.517
CLV_PCSK_SKI1_1	107	111	PF00082	0.474
CLV_PCSK_SKI1_1	76	80	PF00082	0.436
CLV_PCSK_SKI1_1	97	101	PF00082	0.549
DEG_Nend_UBRbox_4	1	3	PF02207	0.407
DEG_SCF_TRCP1_1	215	220	PF00400	0.538
DOC_CKS1_1	194	199	PF01111	0.548
DOC_PP4_FxxP_1	297	300	PF00568	0.452
DOC_SPAK_OSR1_1	168	172	PF12202	0.471
DOC_SPAK_OSR1_1	90	94	PF12202	0.383
DOC_USP7_MATH_1	182	186	PF00917	0.492
DOC_USP7_MATH_1	99	103	PF00917	0.494
DOC_USP7_UBL2_3	110	114	PF12436	0.562
DOC_WW_Pin1_4	193	198	PF00397	0.459
LIG_14-3-3_CanoR_1	139	146	PF00244	0.420
LIG_14-3-3_CanoR_1	324	329	PF00244	0.502
LIG_14-3-3_CanoR_1	62	68	PF00244	0.495
LIG_AP2alpha_1	84	88	PF02296	0.485
LIG_APCC_ABBA_1	22	27	PF00400	0.414
LIG_BRCT_BRCA1_1	223	227	PF00533	0.400
LIG_BRCT_BRCA1_1	3	7	PF00533	0.659
LIG_BRCT_BRCA1_1	60	64	PF00533	0.432
LIG_EH1_1	42	50	PF00400	0.390
LIG_eIF4E_1	10	16	PF01652	0.376
LIG_FHA_1	139	145	PF00498	0.363
LIG_FHA_1	243	249	PF00498	0.463
LIG_FHA_1	276	282	PF00498	0.452
LIG_FHA_2	64	70	PF00498	0.397
LIG_GBD_Chelix_1	11	19	PF00786	0.375
LIG_LIR_Apic_2	296	300	PF02991	0.452
LIG_LIR_Gen_1	224	235	PF02991	0.461
LIG_LIR_Gen_1	267	276	PF02991	0.463
LIG_LIR_Gen_1	61	70	PF02991	0.423
LIG_LIR_Gen_1	86	95	PF02991	0.387
LIG_LIR_Nem_3	267	273	PF02991	0.463
LIG_LIR_Nem_3	4	10	PF02991	0.654
LIG_LIR_Nem_3	61	67	PF02991	0.415
LIG_LIR_Nem_3	86	91	PF02991	0.383
LIG_NRP_CendR_1	334	336	PF00754	0.752
LIG_OCRL_FandH_1	12	24	PF00620	0.388
LIG_Pex14_2	270	274	PF04695	0.452
LIG_Pex14_2	84	88	PF04695	0.485
LIG_PTAP_UEV_1	328	333	PF05743	0.625
LIG_SH2_CRK	209	213	PF00017	0.390
LIG_SH2_STAP1	43	47	PF00017	0.389
LIG_SH2_STAT5	10	13	PF00017	0.379
LIG_SH2_STAT5	170	173	PF00017	0.356
LIG_SH2_STAT5	209	212	PF00017	0.390
LIG_SH2_STAT5	233	236	PF00017	0.462
LIG_SH2_STAT5	262	265	PF00017	0.452
LIG_SH3_2	329	334	PF14604	0.641
LIG_SH3_3	326	332	PF00018	0.614
LIG_SH3_5	39	43	PF00018	0.406
LIG_SUMO_SIM_anti_2	301	309	PF11976	0.452
LIG_SUMO_SIM_anti_2	44	49	PF11976	0.403
LIG_SUMO_SIM_par_1	151	156	PF11976	0.485
LIG_TRAF2_1	160	163	PF00917	0.371
LIG_WRC_WIRS_1	64	69	PF05994	0.525
MOD_CK1_1	216	222	PF00069	0.538
MOD_CK1_1	244	250	PF00069	0.463
MOD_CK1_1	301	307	PF00069	0.470
MOD_CK2_1	157	163	PF00069	0.494
MOD_CK2_1	28	34	PF00069	0.392
MOD_CK2_1	63	69	PF00069	0.417
MOD_Cter_Amidation	332	335	PF01082	0.604
MOD_GlcNHglycan	148	151	PF01048	0.445
MOD_GlcNHglycan	184	187	PF01048	0.446
MOD_GlcNHglycan	215	218	PF01048	0.313
MOD_GlcNHglycan	30	33	PF01048	0.385
MOD_GlcNHglycan	315	318	PF01048	0.368
MOD_GlcNHglycan	329	332	PF01048	0.504
MOD_GSK3_1	11	18	PF00069	0.557
MOD_GSK3_1	153	160	PF00069	0.478
MOD_GSK3_1	213	220	PF00069	0.530
MOD_GSK3_1	301	308	PF00069	0.452
MOD_GSK3_1	65	72	PF00069	0.453
MOD_N-GLC_1	139	144	PF02516	0.451
MOD_N-GLC_1	28	33	PF02516	0.396
MOD_N-GLC_1	93	98	PF02516	0.503
MOD_NEK2_1	1	6	PF00069	0.557
MOD_NEK2_1	105	110	PF00069	0.419
MOD_NEK2_1	15	20	PF00069	0.462
MOD_NEK2_1	153	158	PF00069	0.410
MOD_NEK2_1	241	246	PF00069	0.461
MOD_NEK2_1	264	269	PF00069	0.442
MOD_PIKK_1	123	129	PF00454	0.398
MOD_PKA_2	138	144	PF00069	0.429
MOD_Plk_1	139	145	PF00069	0.519
MOD_Plk_1	43	49	PF00069	0.369
MOD_Plk_2-3	37	43	PF00069	0.487
MOD_Plk_4	1	7	PF00069	0.642
MOD_Plk_4	140	146	PF00069	0.464
MOD_Plk_4	208	214	PF00069	0.290
MOD_Plk_4	217	223	PF00069	0.467
MOD_Plk_4	298	304	PF00069	0.459
MOD_Plk_4	43	49	PF00069	0.487
MOD_ProDKin_1	193	199	PF00069	0.452
MOD_SUMO_rev_2	277	284	PF00179	0.463
TRG_DiLeu_BaEn_1	237	242	PF01217	0.452
TRG_ENDOCYTIC_2	10	13	PF00928	0.379
TRG_ENDOCYTIC_2	209	212	PF00928	0.387
TRG_ENDOCYTIC_2	81	84	PF00928	0.470

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P5D7	Leptomonas seymouri	87%	100%
A0A0S4KHS3	Bodo saltans	54%	100%
A0A1X0NMS5	Trypanosomatidae	70%	100%
A0A3Q8IBD6	Leishmania donovani	98%	100%
A0A3R7NDG3	Trypanosoma rangeli	67%	100%
A4H6R5	Leishmania braziliensis	91%	100%
A4HV45	Leishmania infantum	98%	100%
C9ZQE1	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	68%	90%
E9ANS6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	98%	100%
P34604	Caenorhabditis elegans	24%	100%
Q0IIF2	Bos taurus	31%	100%
Q14232	Homo sapiens	29%	100%
Q4R4V8	Macaca fascicularis	30%	100%
Q54I81	Dictyostelium discoideum	29%	100%
Q57586	Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)	25%	100%
Q5RAR0	Pongo abelii	29%	100%
Q64270	Rattus norvegicus	30%	100%
Q99LC8	Mus musculus	31%	100%
Q9USP0	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	28%	99%
V5BMF9	Trypanosoma cruzi	68%	91%