Cysteine--tRNA ligase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

cysteinyl-tRNA synthetase, putative

Species:

Leishmania major

UniProt:

Q4QGR8_LEIMA

TriTrypDb:

LmjF.12.0250 , LMJLV39_120007900 , LMJSD75_120005500

Length:

784

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 2
Forrest at al. (procyclic)	no	yes: 2
Silverman et al.	yes	yes: 2
Pissara et al.	yes	yes: 8

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	yes	yes: 4

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 4

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

Q4QGR8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QGR8

Function

Biological processes
Term	Name	Level	Count
GO:0006082	organic acid metabolic process	3	11
GO:0006139	nucleobase-containing compound metabolic process	3	11
GO:0006399	tRNA metabolic process	7	11
GO:0006418	tRNA aminoacylation for protein translation	6	11
GO:0006423	cysteinyl-tRNA aminoacylation	7	11
GO:0006520	amino acid metabolic process	3	11
GO:0006725	cellular aromatic compound metabolic process	3	11
GO:0006807	nitrogen compound metabolic process	2	11
GO:0008152	metabolic process	1	11
GO:0009987	cellular process	1	11
GO:0016070	RNA metabolic process	5	11
GO:0019752	carboxylic acid metabolic process	5	11
GO:0034641	cellular nitrogen compound metabolic process	3	11
GO:0034660	ncRNA metabolic process	6	11
GO:0043038	amino acid activation	4	11
GO:0043039	tRNA aminoacylation	5	11
GO:0043170	macromolecule metabolic process	3	11
GO:0043436	oxoacid metabolic process	4	11
GO:0044237	cellular metabolic process	2	11
GO:0044238	primary metabolic process	2	11
GO:0044281	small molecule metabolic process	2	11
GO:0046483	heterocycle metabolic process	3	11
GO:0071704	organic substance metabolic process	2	11
GO:0090304	nucleic acid metabolic process	4	11
GO:1901360	organic cyclic compound metabolic process	3	11
GO:1901564	organonitrogen compound metabolic process	3	11

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	11
GO:0003824	catalytic activity	1	11
GO:0004812	aminoacyl-tRNA ligase activity	4	11
GO:0004817	cysteine-tRNA ligase activity	5	11
GO:0005488	binding	1	11
GO:0005524	ATP binding	5	11
GO:0016874	ligase activity	2	11
GO:0016875	ligase activity, forming carbon-oxygen bonds	3	11
GO:0017076	purine nucleotide binding	4	11
GO:0030554	adenyl nucleotide binding	5	11
GO:0032553	ribonucleotide binding	3	11
GO:0032555	purine ribonucleotide binding	4	11
GO:0032559	adenyl ribonucleotide binding	5	11
GO:0035639	purine ribonucleoside triphosphate binding	4	11
GO:0036094	small molecule binding	2	11
GO:0043167	ion binding	2	11
GO:0043168	anion binding	3	11
GO:0043169	cation binding	3	11
GO:0046872	metal ion binding	4	11
GO:0097159	organic cyclic compound binding	2	11
GO:0097367	carbohydrate derivative binding	2	11
GO:0140098	catalytic activity, acting on RNA	3	11
GO:0140101	catalytic activity, acting on a tRNA	4	11
GO:0140640	catalytic activity, acting on a nucleic acid	2	11
GO:1901265	nucleoside phosphate binding	3	11
GO:1901363	heterocyclic compound binding	2	11

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	154	156	PF00675	0.362
CLV_NRD_NRD_1	236	238	PF00675	0.357
CLV_NRD_NRD_1	559	561	PF00675	0.458
CLV_NRD_NRD_1	667	669	PF00675	0.499
CLV_NRD_NRD_1	677	679	PF00675	0.485
CLV_NRD_NRD_1	686	688	PF00675	0.448
CLV_NRD_NRD_1	705	707	PF00675	0.475
CLV_NRD_NRD_1	87	89	PF00675	0.303
CLV_PCSK_FUR_1	675	679	PF00082	0.507
CLV_PCSK_KEX2_1	113	115	PF00082	0.329
CLV_PCSK_KEX2_1	154	156	PF00082	0.362
CLV_PCSK_KEX2_1	20	22	PF00082	0.508
CLV_PCSK_KEX2_1	333	335	PF00082	0.316
CLV_PCSK_KEX2_1	667	669	PF00082	0.650
CLV_PCSK_KEX2_1	677	679	PF00082	0.456
CLV_PCSK_KEX2_1	686	688	PF00082	0.448
CLV_PCSK_KEX2_1	705	707	PF00082	0.475
CLV_PCSK_KEX2_1	87	89	PF00082	0.303
CLV_PCSK_PC1ET2_1	113	115	PF00082	0.303
CLV_PCSK_PC1ET2_1	154	156	PF00082	0.397
CLV_PCSK_PC1ET2_1	20	22	PF00082	0.533
CLV_PCSK_PC1ET2_1	333	335	PF00082	0.316
CLV_PCSK_PC1ET2_1	686	688	PF00082	0.474
CLV_PCSK_SKI1_1	110	114	PF00082	0.303
CLV_PCSK_SKI1_1	156	160	PF00082	0.437
CLV_PCSK_SKI1_1	241	245	PF00082	0.328
CLV_PCSK_SKI1_1	422	426	PF00082	0.303
CLV_PCSK_SKI1_1	428	432	PF00082	0.303
CLV_PCSK_SKI1_1	437	441	PF00082	0.303
CLV_PCSK_SKI1_1	451	455	PF00082	0.303
CLV_PCSK_SKI1_1	481	485	PF00082	0.388
CLV_PCSK_SKI1_1	5	9	PF00082	0.503
CLV_PCSK_SKI1_1	507	511	PF00082	0.531
CLV_PCSK_SKI1_1	694	698	PF00082	0.627
CLV_PCSK_SKI1_1	738	742	PF00082	0.542
CLV_PCSK_SKI1_1	749	753	PF00082	0.458
DEG_APCC_DBOX_1	436	444	PF00400	0.516
DOC_MAPK_FxFP_2	696	699	PF00069	0.438
DOC_MAPK_gen_1	113	122	PF00069	0.536
DOC_MAPK_gen_1	172	182	PF00069	0.518
DOC_MAPK_gen_1	428	436	PF00069	0.503
DOC_MAPK_MEF2A_6	116	124	PF00069	0.454
DOC_MAPK_MEF2A_6	175	184	PF00069	0.538
DOC_PP1_RVXF_1	449	455	PF00149	0.503
DOC_PP1_RVXF_1	55	62	PF00149	0.500
DOC_PP4_FxxP_1	344	347	PF00568	0.503
DOC_PP4_FxxP_1	696	699	PF00568	0.486
DOC_USP7_MATH_1	147	151	PF00917	0.550
DOC_USP7_MATH_1	208	212	PF00917	0.562
DOC_USP7_UBL2_3	688	692	PF12436	0.438
DOC_USP7_UBL2_3	738	742	PF12436	0.508
DOC_USP7_UBL2_3	745	749	PF12436	0.470
DOC_WW_Pin1_4	332	337	PF00397	0.543
DOC_WW_Pin1_4	351	356	PF00397	0.503
LIG_14-3-3_CanoR_1	130	138	PF00244	0.642
LIG_14-3-3_CanoR_1	221	227	PF00244	0.523
LIG_14-3-3_CanoR_1	260	268	PF00244	0.525
LIG_14-3-3_CanoR_1	311	318	PF00244	0.559
LIG_14-3-3_CanoR_1	5	10	PF00244	0.391
LIG_14-3-3_CanoR_1	553	559	PF00244	0.429
LIG_14-3-3_CanoR_1	60	69	PF00244	0.546
LIG_14-3-3_CanoR_1	694	699	PF00244	0.523
LIG_14-3-3_CanoR_1	78	84	PF00244	0.513
LIG_ActinCP_TwfCPI_2	344	351	PF01115	0.534
LIG_BIR_II_1	1	5	PF00653	0.598
LIG_BRCT_BRCA1_1	286	290	PF00533	0.510
LIG_BRCT_BRCA1_1	700	704	PF00533	0.515
LIG_deltaCOP1_diTrp_1	349	356	PF00928	0.503
LIG_deltaCOP1_diTrp_1	691	696	PF00928	0.577
LIG_FHA_1	1	7	PF00498	0.629
LIG_FHA_1	260	266	PF00498	0.503
LIG_FHA_1	43	49	PF00498	0.454
LIG_FHA_1	447	453	PF00498	0.538
LIG_FHA_1	497	503	PF00498	0.480
LIG_FHA_1	80	86	PF00498	0.519
LIG_FHA_2	103	109	PF00498	0.503
LIG_FHA_2	311	317	PF00498	0.508
LIG_FHA_2	45	51	PF00498	0.572
LIG_FHA_2	65	71	PF00498	0.427
LIG_FHA_2	715	721	PF00498	0.578
LIG_LIR_Apic_2	349	355	PF02991	0.503
LIG_LIR_Gen_1	201	210	PF02991	0.519
LIG_LIR_Gen_1	526	534	PF02991	0.508
LIG_LIR_Gen_1	593	604	PF02991	0.627
LIG_LIR_Gen_1	717	726	PF02991	0.598
LIG_LIR_Gen_1	753	758	PF02991	0.523
LIG_LIR_Gen_1	82	90	PF02991	0.503
LIG_LIR_Nem_3	201	206	PF02991	0.505
LIG_LIR_Nem_3	301	305	PF02991	0.516
LIG_LIR_Nem_3	407	412	PF02991	0.577
LIG_LIR_Nem_3	526	531	PF02991	0.514
LIG_LIR_Nem_3	567	573	PF02991	0.443
LIG_LIR_Nem_3	593	599	PF02991	0.648
LIG_LIR_Nem_3	691	696	PF02991	0.524
LIG_LIR_Nem_3	713	719	PF02991	0.588
LIG_LIR_Nem_3	753	757	PF02991	0.520
LIG_LIR_Nem_3	82	86	PF02991	0.503
LIG_LIR_Nem_3	91	96	PF02991	0.503
LIG_LYPXL_yS_3	28	31	PF13949	0.505
LIG_LYPXL_yS_3	302	305	PF13949	0.516
LIG_MLH1_MIPbox_1	286	290	PF16413	0.503
LIG_MLH1_MIPbox_1	700	704	PF16413	0.515
LIG_MYND_1	303	307	PF01753	0.516
LIG_PCNA_TLS_4	87	94	PF02747	0.503
LIG_Pex14_1	386	390	PF04695	0.516
LIG_Pex14_1	409	413	PF04695	0.503
LIG_Pex14_2	203	207	PF04695	0.503
LIG_SH2_CRK	96	100	PF00017	0.516
LIG_SH2_NCK_1	239	243	PF00017	0.597
LIG_SH2_NCK_1	719	723	PF00017	0.588
LIG_SH2_NCK_1	96	100	PF00017	0.584
LIG_SH2_SRC	719	722	PF00017	0.572
LIG_SH2_STAP1	239	243	PF00017	0.516
LIG_SH2_STAP1	750	754	PF00017	0.518
LIG_SH2_STAP1	96	100	PF00017	0.559
LIG_SH2_STAT3	264	267	PF00017	0.534
LIG_SH2_STAT5	264	267	PF00017	0.503
LIG_SH2_STAT5	295	298	PF00017	0.602
LIG_SH2_STAT5	412	415	PF00017	0.531
LIG_SH2_STAT5	547	550	PF00017	0.428
LIG_SH2_STAT5	565	568	PF00017	0.369
LIG_SH2_STAT5	62	65	PF00017	0.516
LIG_SH2_STAT5	703	706	PF00017	0.480
LIG_SH2_STAT5	716	719	PF00017	0.441
LIG_SH2_STAT5	80	83	PF00017	0.503
LIG_SH2_STAT5	93	96	PF00017	0.503
LIG_SH3_3	344	350	PF00018	0.528
LIG_SH3_3	643	649	PF00018	0.562
LIG_SUMO_SIM_anti_2	486	491	PF11976	0.435
LIG_TRAF2_1	669	672	PF00917	0.583
LIG_TRAF2_1	731	734	PF00917	0.611
LIG_TYR_ITSM	715	722	PF00017	0.463
LIG_WRC_WIRS_1	80	85	PF05994	0.340
MOD_CK1_1	222	228	PF00069	0.368
MOD_CK1_1	466	472	PF00069	0.359
MOD_CK2_1	311	317	PF00069	0.370
MOD_CK2_1	40	46	PF00069	0.401
MOD_CK2_1	466	472	PF00069	0.340
MOD_CK2_1	733	739	PF00069	0.597
MOD_CK2_1	750	756	PF00069	0.466
MOD_GlcNHglycan	149	152	PF01048	0.435
MOD_GlcNHglycan	210	213	PF01048	0.433
MOD_GlcNHglycan	219	224	PF01048	0.432
MOD_GlcNHglycan	382	385	PF01048	0.361
MOD_GlcNHglycan	549	552	PF01048	0.497
MOD_GlcNHglycan	554	557	PF01048	0.402
MOD_GSK3_1	365	372	PF00069	0.373
MOD_GSK3_1	40	47	PF00069	0.449
MOD_GSK3_1	60	67	PF00069	0.211
MOD_GSK3_1	647	654	PF00069	0.636
MOD_GSK3_1	694	701	PF00069	0.540
MOD_GSK3_1	720	727	PF00069	0.494
MOD_N-GLC_1	102	107	PF02516	0.340
MOD_N-GLC_1	311	316	PF02516	0.340
MOD_N-GLC_1	463	468	PF02516	0.359
MOD_N-GLC_2	395	397	PF02516	0.340
MOD_NEK2_1	184	189	PF00069	0.396
MOD_NEK2_1	424	429	PF00069	0.340
MOD_NEK2_1	443	448	PF00069	0.211
MOD_NEK2_1	94	99	PF00069	0.472
MOD_NEK2_2	284	289	PF00069	0.340
MOD_PIKK_1	184	190	PF00454	0.389
MOD_PIKK_1	404	410	PF00454	0.453
MOD_PIKK_1	496	502	PF00454	0.532
MOD_PKA_1	714	720	PF00069	0.452
MOD_PKA_1	741	747	PF00069	0.518
MOD_PKA_2	129	135	PF00069	0.511
MOD_PKA_2	259	265	PF00069	0.369
MOD_PKA_2	310	316	PF00069	0.472
MOD_PKA_2	552	558	PF00069	0.423
MOD_Plk_1	284	290	PF00069	0.384
MOD_Plk_1	365	371	PF00069	0.340
MOD_Plk_1	466	472	PF00069	0.340
MOD_Plk_1	69	75	PF00069	0.359
MOD_Plk_2-3	733	739	PF00069	0.589
MOD_Plk_4	284	290	PF00069	0.355
MOD_Plk_4	365	371	PF00069	0.340
MOD_Plk_4	5	11	PF00069	0.493
MOD_Plk_4	527	533	PF00069	0.478
MOD_Plk_4	536	542	PF00069	0.409
MOD_Plk_4	554	560	PF00069	0.301
MOD_Plk_4	599	605	PF00069	0.463
MOD_Plk_4	64	70	PF00069	0.367
MOD_Plk_4	761	767	PF00069	0.605
MOD_Plk_4	772	778	PF00069	0.598
MOD_Plk_4	94	100	PF00069	0.500
MOD_ProDKin_1	332	338	PF00069	0.396
MOD_ProDKin_1	351	357	PF00069	0.340
MOD_SUMO_for_1	124	127	PF00179	0.271
MOD_SUMO_for_1	19	22	PF00179	0.500
MOD_SUMO_rev_2	106	112	PF00179	0.340
MOD_SUMO_rev_2	174	180	PF00179	0.341
MOD_SUMO_rev_2	186	194	PF00179	0.342
MOD_SUMO_rev_2	383	391	PF00179	0.359
MOD_SUMO_rev_2	708	717	PF00179	0.570
MOD_SUMO_rev_2	733	743	PF00179	0.525
TRG_DiLeu_BaLyEn_6	448	453	PF01217	0.340
TRG_ENDOCYTIC_2	28	31	PF00928	0.505
TRG_ENDOCYTIC_2	302	305	PF00928	0.340
TRG_ENDOCYTIC_2	719	722	PF00928	0.510
TRG_ENDOCYTIC_2	754	757	PF00928	0.488
TRG_ENDOCYTIC_2	80	83	PF00928	0.340
TRG_ENDOCYTIC_2	96	99	PF00928	0.340
TRG_ER_diArg_1	114	117	PF00400	0.373
TRG_ER_diArg_1	675	678	PF00400	0.627
TRG_ER_diArg_1	704	706	PF00400	0.557
TRG_ER_diArg_1	86	88	PF00400	0.340
TRG_NLS_MonoExtC_3	685	691	PF00514	0.475
TRG_NLS_MonoExtN_4	110	117	PF00514	0.340
TRG_Pf-PMV_PEXEL_1	662	666	PF00026	0.566
TRG_Pf-PMV_PEXEL_1	677	682	PF00026	0.438
TRG_Pf-PMV_PEXEL_1	687	691	PF00026	0.476
TRG_Pf-PMV_PEXEL_1	87	91	PF00026	0.307

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P2U0	Leptomonas seymouri	82%	99%
A0A0S4JIJ5	Bodo saltans	64%	100%
A0A1X0NM71	Trypanosomatidae	69%	98%
A0A3S7WRW6	Leishmania donovani	97%	100%
A0A422N5J6	Trypanosoma rangeli	68%	100%
A4H6U0	Leishmania braziliensis	89%	100%
C9ZQB2	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	65%	100%
E9AGB4	Leishmania infantum	97%	100%
E9ANV2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	96%	100%
P49589	Homo sapiens	41%	100%
P53852	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	40%	100%
Q09860	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	39%	100%
Q291L4	Drosophila pseudoobscura pseudoobscura	40%	100%
Q4R550	Macaca fascicularis	41%	100%
Q54KR1	Dictyostelium discoideum	44%	100%
Q5F408	Gallus gallus	40%	100%
Q5M7N8	Xenopus tropicalis	41%	100%
Q7KN90	Drosophila melanogaster	40%	100%
Q7ZWR2	Xenopus laevis	41%	100%
Q9ER72	Mus musculus	39%	94%