Putative N(2), N(2)-dimethylguanosine tRNA methyltransferase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

N(2), N(2)-dimethylguanosine tRNA methyltransferase, putative

Species:

Leishmania major

UniProt:

Q4QG60_LEIMA

TriTrypDb:

LmjF.13.0940 , LMJLV39_130013500 , LMJSD75_130013600

Length:

718

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

Q4QG60

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QG60

Function

Biological processes
Term	Name	Level	Count
GO:0001510	RNA methylation	4	2
GO:0002940	tRNA N2-guanine methylation	6	2
GO:0006139	nucleobase-containing compound metabolic process	3	2
GO:0006396	RNA processing	6	2
GO:0006399	tRNA metabolic process	7	2
GO:0006400	tRNA modification	6	2
GO:0006725	cellular aromatic compound metabolic process	3	2
GO:0006807	nitrogen compound metabolic process	2	2
GO:0008033	tRNA processing	8	2
GO:0008152	metabolic process	1	2
GO:0009451	RNA modification	5	2
GO:0009987	cellular process	1	2
GO:0016070	RNA metabolic process	5	2
GO:0030488	tRNA methylation	5	2
GO:0032259	methylation	2	2
GO:0034470	ncRNA processing	7	2
GO:0034641	cellular nitrogen compound metabolic process	3	2
GO:0034660	ncRNA metabolic process	6	2
GO:0043170	macromolecule metabolic process	3	2
GO:0043412	macromolecule modification	4	2
GO:0043414	macromolecule methylation	3	2
GO:0044237	cellular metabolic process	2	2
GO:0044238	primary metabolic process	2	2
GO:0044260	obsolete cellular macromolecule metabolic process	3	2
GO:0046483	heterocycle metabolic process	3	2
GO:0071704	organic substance metabolic process	2	2
GO:0090304	nucleic acid metabolic process	4	2
GO:1901360	organic cyclic compound metabolic process	3	2

Molecular functions
Term	Name	Level	Count
GO:0000049	tRNA binding	5	12
GO:0003676	nucleic acid binding	3	12
GO:0003723	RNA binding	4	12
GO:0003824	catalytic activity	1	12
GO:0004809	tRNA (guanine-N2-)-methyltransferase activity	6	12
GO:0005488	binding	1	12
GO:0008168	methyltransferase activity	4	12
GO:0008170	N-methyltransferase activity	5	12
GO:0008173	RNA methyltransferase activity	4	12
GO:0008175	tRNA methyltransferase activity	5	12
GO:0008757	S-adenosylmethionine-dependent methyltransferase activity	5	12
GO:0016423	tRNA (guanine) methyltransferase activity	6	12
GO:0016740	transferase activity	2	12
GO:0016741	transferase activity, transferring one-carbon groups	3	12
GO:0097159	organic cyclic compound binding	2	12
GO:0140098	catalytic activity, acting on RNA	3	12
GO:0140101	catalytic activity, acting on a tRNA	4	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	12
GO:1901363	heterocyclic compound binding	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	280	284	PF00656	0.307
CLV_C14_Caspase3-7	56	60	PF00656	0.660
CLV_C14_Caspase3-7	709	713	PF00656	0.737
CLV_NRD_NRD_1	395	397	PF00675	0.543
CLV_NRD_NRD_1	398	400	PF00675	0.554
CLV_NRD_NRD_1	433	435	PF00675	0.659
CLV_NRD_NRD_1	691	693	PF00675	0.606
CLV_NRD_NRD_1	97	99	PF00675	0.328
CLV_PCSK_FUR_1	396	400	PF00082	0.604
CLV_PCSK_FUR_1	431	435	PF00082	0.653
CLV_PCSK_KEX2_1	223	225	PF00082	0.625
CLV_PCSK_KEX2_1	394	396	PF00082	0.539
CLV_PCSK_KEX2_1	398	400	PF00082	0.549
CLV_PCSK_KEX2_1	433	435	PF00082	0.781
CLV_PCSK_KEX2_1	437	439	PF00082	0.794
CLV_PCSK_KEX2_1	690	692	PF00082	0.597
CLV_PCSK_KEX2_1	96	98	PF00082	0.343
CLV_PCSK_PC1ET2_1	223	225	PF00082	0.687
CLV_PCSK_PC1ET2_1	437	439	PF00082	0.795
CLV_PCSK_PC7_1	391	397	PF00082	0.659
CLV_PCSK_PC7_1	433	439	PF00082	0.778
CLV_PCSK_SKI1_1	123	127	PF00082	0.397
CLV_PCSK_SKI1_1	241	245	PF00082	0.392
CLV_PCSK_SKI1_1	269	273	PF00082	0.342
CLV_PCSK_SKI1_1	315	319	PF00082	0.294
CLV_PCSK_SKI1_1	345	349	PF00082	0.358
CLV_PCSK_SKI1_1	398	402	PF00082	0.624
CLV_PCSK_SKI1_1	547	551	PF00082	0.391
CLV_PCSK_SKI1_1	611	615	PF00082	0.342
CLV_PCSK_SKI1_1	650	654	PF00082	0.587
DEG_APCC_DBOX_1	314	322	PF00400	0.323
DEG_APCC_DBOX_1	546	554	PF00400	0.427
DEG_SCF_FBW7_2	652	659	PF00400	0.526
DEG_SPOP_SBC_1	487	491	PF00917	0.318
DOC_CKS1_1	574	579	PF01111	0.323
DOC_CKS1_1	653	658	PF01111	0.524
DOC_CYCLIN_RxL_1	310	322	PF00134	0.307
DOC_CYCLIN_RxL_1	647	655	PF00134	0.588
DOC_CYCLIN_yCln2_LP_2	505	511	PF00134	0.364
DOC_MAPK_DCC_7	330	338	PF00069	0.310
DOC_MAPK_DCC_7	601	610	PF00069	0.319
DOC_MAPK_gen_1	269	279	PF00069	0.321
DOC_MAPK_gen_1	327	335	PF00069	0.307
DOC_MAPK_MEF2A_6	330	338	PF00069	0.310
DOC_MAPK_MEF2A_6	541	550	PF00069	0.319
DOC_MAPK_MEF2A_6	601	610	PF00069	0.319
DOC_PP2B_LxvP_1	32	35	PF13499	0.459
DOC_PP4_FxxP_1	331	334	PF00568	0.415
DOC_PP4_FxxP_1	68	71	PF00568	0.307
DOC_USP7_MATH_1	214	218	PF00917	0.582
DOC_USP7_MATH_1	232	236	PF00917	0.427
DOC_USP7_MATH_1	256	260	PF00917	0.323
DOC_USP7_MATH_1	387	391	PF00917	0.669
DOC_USP7_MATH_1	423	427	PF00917	0.763
DOC_USP7_MATH_1	463	467	PF00917	0.646
DOC_USP7_MATH_1	635	639	PF00917	0.723
DOC_USP7_MATH_1	643	647	PF00917	0.661
DOC_USP7_MATH_1	682	686	PF00917	0.646
DOC_USP7_UBL2_3	637	641	PF12436	0.646
DOC_WW_Pin1_4	14	19	PF00397	0.524
DOC_WW_Pin1_4	185	190	PF00397	0.661
DOC_WW_Pin1_4	258	263	PF00397	0.307
DOC_WW_Pin1_4	461	466	PF00397	0.627
DOC_WW_Pin1_4	471	476	PF00397	0.501
DOC_WW_Pin1_4	573	578	PF00397	0.323
DOC_WW_Pin1_4	652	657	PF00397	0.484
LIG_14-3-3_CanoR_1	379	389	PF00244	0.427
LIG_14-3-3_CanoR_1	467	471	PF00244	0.641
LIG_14-3-3_CanoR_1	530	536	PF00244	0.418
LIG_14-3-3_CanoR_1	89	95	PF00244	0.415
LIG_Actin_WH2_2	101	119	PF00022	0.308
LIG_Actin_WH2_2	76	93	PF00022	0.454
LIG_APCC_ABBA_1	336	341	PF00400	0.307
LIG_BIR_II_1	1	5	PF00653	0.557
LIG_BRCT_BRCA1_1	4	8	PF00533	0.434
LIG_FHA_1	235	241	PF00498	0.371
LIG_FHA_1	26	32	PF00498	0.436
LIG_FHA_1	541	547	PF00498	0.398
LIG_FHA_1	574	580	PF00498	0.364
LIG_FHA_2	104	110	PF00498	0.307
LIG_FHA_2	278	284	PF00498	0.307
LIG_FHA_2	616	622	PF00498	0.359
LIG_FHA_2	628	634	PF00498	0.679
LIG_GSK3_LRP6_1	573	578	PF00069	0.312
LIG_IRF3_LxIS_1	75	82	PF10401	0.422
LIG_LIR_Apic_2	374	380	PF02991	0.454
LIG_LIR_Gen_1	567	575	PF02991	0.308
LIG_LIR_Gen_1	85	95	PF02991	0.364
LIG_LIR_Nem_3	265	271	PF02991	0.392
LIG_LIR_Nem_3	567	571	PF02991	0.331
LIG_LIR_Nem_3	85	90	PF02991	0.364
LIG_LYPXL_yS_3	157	160	PF13949	0.427
LIG_NRBOX	519	525	PF00104	0.307
LIG_PCNA_yPIPBox_3	241	252	PF02747	0.368
LIG_PTB_Apo_2	124	131	PF02174	0.427
LIG_PTB_Apo_2	144	151	PF02174	0.123
LIG_PTB_Phospho_1	124	130	PF10480	0.427
LIG_PTB_Phospho_1	144	150	PF10480	0.123
LIG_REV1ctd_RIR_1	345	353	PF16727	0.364
LIG_SH2_CRK	10	14	PF00017	0.528
LIG_SH2_CRK	150	154	PF00017	0.427
LIG_SH2_CRK	351	355	PF00017	0.323
LIG_SH2_CRK	510	514	PF00017	0.323
LIG_SH2_NCK_1	10	14	PF00017	0.497
LIG_SH2_NCK_1	510	514	PF00017	0.307
LIG_SH2_NCK_1	69	73	PF00017	0.307
LIG_SH2_PTP2	568	571	PF00017	0.427
LIG_SH2_SRC	130	133	PF00017	0.364
LIG_SH2_STAP1	10	14	PF00017	0.576
LIG_SH2_STAT3	366	369	PF00017	0.323
LIG_SH2_STAT5	130	133	PF00017	0.372
LIG_SH2_STAT5	343	346	PF00017	0.342
LIG_SH2_STAT5	560	563	PF00017	0.441
LIG_SH2_STAT5	568	571	PF00017	0.411
LIG_SH3_2	462	467	PF14604	0.573
LIG_SH3_3	152	158	PF00018	0.425
LIG_SH3_3	225	231	PF00018	0.630
LIG_SH3_3	28	34	PF00018	0.416
LIG_SH3_3	459	465	PF00018	0.562
LIG_SH3_3	551	557	PF00018	0.335
LIG_SH3_3	568	574	PF00018	0.220
LIG_SUMO_SIM_anti_2	103	109	PF11976	0.392
LIG_SUMO_SIM_anti_2	80	85	PF11976	0.415
LIG_SUMO_SIM_par_1	250	255	PF11976	0.314
LIG_SUMO_SIM_par_1	82	88	PF11976	0.454
LIG_TRAF2_1	390	393	PF00917	0.608
LIG_TRAF2_1	412	415	PF00917	0.738
LIG_TRAF2_1	54	57	PF00917	0.729
LIG_TRAF2_1	549	552	PF00917	0.323
LIG_TRFH_1	559	563	PF08558	0.454
LIG_TYR_ITIM	155	160	PF00017	0.454
LIG_UBA3_1	609	617	PF00899	0.374
MOD_CDC14_SPxK_1	464	467	PF00782	0.688
MOD_CDK_SPK_2	185	190	PF00069	0.659
MOD_CDK_SPxK_1	461	467	PF00069	0.651
MOD_CK1_1	2	8	PF00069	0.560
MOD_CK1_1	281	287	PF00069	0.323
MOD_CK1_1	371	377	PF00069	0.463
MOD_CK1_1	466	472	PF00069	0.590
MOD_CK1_1	483	489	PF00069	0.370
MOD_CK1_1	51	57	PF00069	0.707
MOD_CK2_1	103	109	PF00069	0.307
MOD_CK2_1	387	393	PF00069	0.631
MOD_CK2_1	51	57	PF00069	0.711
MOD_CK2_1	79	85	PF00069	0.422
MOD_Cter_Amidation	221	224	PF01082	0.624
MOD_GlcNHglycan	111	114	PF01048	0.307
MOD_GlcNHglycan	182	185	PF01048	0.674
MOD_GlcNHglycan	233	237	PF01048	0.459
MOD_GlcNHglycan	258	261	PF01048	0.312
MOD_GlcNHglycan	283	286	PF01048	0.310
MOD_GlcNHglycan	359	362	PF01048	0.427
MOD_GlcNHglycan	373	376	PF01048	0.427
MOD_GlcNHglycan	4	7	PF01048	0.437
MOD_GlcNHglycan	43	46	PF01048	0.676
MOD_GlcNHglycan	482	485	PF01048	0.614
MOD_GlcNHglycan	505	508	PF01048	0.315
MOD_GlcNHglycan	684	687	PF01048	0.658
MOD_GlcNHglycan	714	717	PF01048	0.801
MOD_GSK3_1	22	29	PF00069	0.532
MOD_GSK3_1	232	239	PF00069	0.322
MOD_GSK3_1	277	284	PF00069	0.305
MOD_GSK3_1	37	44	PF00069	0.620
MOD_GSK3_1	483	490	PF00069	0.599
MOD_GSK3_1	499	506	PF00069	0.341
MOD_GSK3_1	615	622	PF00069	0.550
MOD_GSK3_1	644	651	PF00069	0.648
MOD_LATS_1	296	302	PF00433	0.307
MOD_N-GLC_1	51	56	PF02516	0.727
MOD_N-GLC_1	682	687	PF02516	0.439
MOD_NEK2_1	111	116	PF00069	0.306
MOD_NEK2_1	277	282	PF00069	0.321
MOD_NEK2_1	291	296	PF00069	0.307
MOD_NEK2_1	368	373	PF00069	0.364
MOD_NEK2_1	79	84	PF00069	0.409
MOD_NEK2_2	236	241	PF00069	0.220
MOD_NEK2_2	334	339	PF00069	0.307
MOD_PIKK_1	511	517	PF00454	0.314
MOD_PIKK_1	588	594	PF00454	0.307
MOD_PK_1	77	83	PF00069	0.307
MOD_PKA_1	37	43	PF00069	0.591
MOD_PKA_1	433	439	PF00069	0.661
MOD_PKA_2	387	393	PF00069	0.638
MOD_PKA_2	433	439	PF00069	0.700
MOD_PKA_2	466	472	PF00069	0.609
MOD_PKA_2	529	535	PF00069	0.359
MOD_PKA_2	540	546	PF00069	0.325
MOD_PKA_2	588	594	PF00069	0.323
MOD_PKA_2	672	678	PF00069	0.583
MOD_PKA_2	90	96	PF00069	0.374
MOD_PKB_1	431	439	PF00069	0.658
MOD_PKB_1	96	104	PF00069	0.427
MOD_Plk_1	627	633	PF00069	0.613
MOD_Plk_1	660	666	PF00069	0.411
MOD_Plk_1	77	83	PF00069	0.318
MOD_Plk_2-3	103	109	PF00069	0.392
MOD_Plk_4	156	162	PF00069	0.376
MOD_Plk_4	499	505	PF00069	0.389
MOD_Plk_4	564	570	PF00069	0.294
MOD_ProDKin_1	14	20	PF00069	0.524
MOD_ProDKin_1	185	191	PF00069	0.648
MOD_ProDKin_1	258	264	PF00069	0.307
MOD_ProDKin_1	461	467	PF00069	0.630
MOD_ProDKin_1	471	477	PF00069	0.503
MOD_ProDKin_1	573	579	PF00069	0.323
MOD_ProDKin_1	652	658	PF00069	0.489
MOD_SUMO_rev_2	410	419	PF00179	0.745
TRG_DiLeu_BaLyEn_6	239	244	PF01217	0.336
TRG_DiLeu_BaLyEn_6	331	336	PF01217	0.307
TRG_ENDOCYTIC_2	10	13	PF00928	0.521
TRG_ENDOCYTIC_2	122	125	PF00928	0.331
TRG_ENDOCYTIC_2	150	153	PF00928	0.427
TRG_ENDOCYTIC_2	157	160	PF00928	0.427
TRG_ENDOCYTIC_2	299	302	PF00928	0.307
TRG_ENDOCYTIC_2	568	571	PF00928	0.427
TRG_ER_diArg_1	394	396	PF00400	0.613
TRG_ER_diArg_1	431	434	PF00400	0.653
TRG_ER_diArg_1	569	572	PF00400	0.304
TRG_ER_diArg_1	670	673	PF00400	0.567
TRG_ER_diArg_1	689	692	PF00400	0.706
TRG_ER_diArg_1	89	92	PF00400	0.318
TRG_ER_diArg_1	95	98	PF00400	0.305
TRG_NLS_MonoExtC_3	638	643	PF00514	0.699
TRG_NLS_MonoExtN_4	34	41	PF00514	0.480
TRG_NLS_MonoExtN_4	637	643	PF00514	0.672
TRG_Pf-PMV_PEXEL_1	241	245	PF00026	0.364
TRG_Pf-PMV_PEXEL_1	269	273	PF00026	0.342

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PF43	Leptomonas seymouri	67%	98%
A0A0S4IT52	Bodo saltans	48%	100%
A0A1X0NNC1	Trypanosomatidae	49%	95%
A0A3R7KAT5	Trypanosoma rangeli	52%	100%
A0A3S7WSL8	Leishmania donovani	93%	99%
A4H782	Leishmania braziliensis	80%	98%
A4HVM2	Leishmania infantum	93%	99%
D0A6N2	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	50%	99%
E9APB8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	90%	99%
Q4R6C7	Macaca fascicularis	21%	100%
V5BB27	Trypanosoma cruzi	53%	99%