TPK_B1_binding domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Thiamin pyrophosphokinase, vitamin B1 binding domain containing protein, putative

Species:

Leishmania major

UniProt:

Q4QG30_LEIMA

TriTrypDb:

LmjF.13.1240 , LMJLV39_130016600 , LMJSD75_130016600

Length:

578

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Could not find GO cellular_component term for this entry.

Expansion

Sequence features

Q4QG30

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QG30

Function

Biological processes
Term	Name	Level	Count
GO:0006725	cellular aromatic compound metabolic process	3	11
GO:0006766	vitamin metabolic process	3	11
GO:0006767	water-soluble vitamin metabolic process	4	11
GO:0006790	sulfur compound metabolic process	3	11
GO:0006793	phosphorus metabolic process	3	11
GO:0006796	phosphate-containing compound metabolic process	4	11
GO:0006807	nitrogen compound metabolic process	2	11
GO:0008152	metabolic process	1	11
GO:0009058	biosynthetic process	2	11
GO:0009110	vitamin biosynthetic process	4	11
GO:0009229	thiamine diphosphate biosynthetic process	5	11
GO:0009987	cellular process	1	11
GO:0016310	phosphorylation	5	10
GO:0018130	heterocycle biosynthetic process	4	11
GO:0019438	aromatic compound biosynthetic process	4	11
GO:0019637	organophosphate metabolic process	3	11
GO:0034641	cellular nitrogen compound metabolic process	3	11
GO:0042357	thiamine diphosphate metabolic process	4	11
GO:0042364	water-soluble vitamin biosynthetic process	5	11
GO:0042723	thiamine-containing compound metabolic process	4	11
GO:0042724	thiamine-containing compound biosynthetic process	5	11
GO:0044237	cellular metabolic process	2	11
GO:0044249	cellular biosynthetic process	3	11
GO:0044271	cellular nitrogen compound biosynthetic process	4	11
GO:0044272	sulfur compound biosynthetic process	4	11
GO:0044281	small molecule metabolic process	2	11
GO:0044283	small molecule biosynthetic process	3	11
GO:0046483	heterocycle metabolic process	3	11
GO:0071704	organic substance metabolic process	2	11
GO:0072527	pyrimidine-containing compound metabolic process	4	11
GO:0072528	pyrimidine-containing compound biosynthetic process	5	11
GO:0090407	organophosphate biosynthetic process	4	11
GO:1901360	organic cyclic compound metabolic process	3	11
GO:1901362	organic cyclic compound biosynthetic process	4	11
GO:1901564	organonitrogen compound metabolic process	3	11
GO:1901566	organonitrogen compound biosynthetic process	4	11
GO:1901576	organic substance biosynthetic process	3	11

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	11
GO:0003824	catalytic activity	1	11
GO:0004788	thiamine diphosphokinase activity	5	11
GO:0005488	binding	1	11
GO:0005524	ATP binding	5	11
GO:0016301	kinase activity	4	10
GO:0016740	transferase activity	2	11
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	11
GO:0016778	diphosphotransferase activity	4	11
GO:0017076	purine nucleotide binding	4	11
GO:0019842	vitamin binding	3	11
GO:0030554	adenyl nucleotide binding	5	11
GO:0030975	thiamine binding	3	11
GO:0032553	ribonucleotide binding	3	11
GO:0032555	purine ribonucleotide binding	4	11
GO:0032559	adenyl ribonucleotide binding	5	11
GO:0035639	purine ribonucleoside triphosphate binding	4	11
GO:0036094	small molecule binding	2	11
GO:0043167	ion binding	2	11
GO:0043168	anion binding	3	11
GO:0043169	cation binding	3	11
GO:0043178	alcohol binding	3	11
GO:0097159	organic cyclic compound binding	2	11
GO:0097367	carbohydrate derivative binding	2	11
GO:1901265	nucleoside phosphate binding	3	11
GO:1901363	heterocyclic compound binding	2	11
GO:1901681	sulfur compound binding	2	11

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	122	126	PF00656	0.536
CLV_C14_Caspase3-7	202	206	PF00656	0.502
CLV_NRD_NRD_1	221	223	PF00675	0.664
CLV_NRD_NRD_1	255	257	PF00675	0.537
CLV_NRD_NRD_1	5	7	PF00675	0.530
CLV_NRD_NRD_1	52	54	PF00675	0.414
CLV_PCSK_FUR_1	253	257	PF00082	0.539
CLV_PCSK_KEX2_1	220	222	PF00082	0.665
CLV_PCSK_KEX2_1	233	235	PF00082	0.531
CLV_PCSK_KEX2_1	253	255	PF00082	0.542
CLV_PCSK_KEX2_1	5	7	PF00082	0.545
CLV_PCSK_PC1ET2_1	233	235	PF00082	0.531
CLV_PCSK_PC1ET2_1	5	7	PF00082	0.569
CLV_PCSK_SKI1_1	234	238	PF00082	0.483
CLV_PCSK_SKI1_1	364	368	PF00082	0.443
CLV_PCSK_SKI1_1	502	506	PF00082	0.348
CLV_Separin_Metazoa	217	221	PF03568	0.618
DEG_APCC_DBOX_1	363	371	PF00400	0.436
DOC_CYCLIN_RxL_1	361	371	PF00134	0.440
DOC_MAPK_MEF2A_6	297	305	PF00069	0.488
DOC_MAPK_MEF2A_6	388	395	PF00069	0.525
DOC_PP1_RVXF_1	231	238	PF00149	0.526
DOC_PP1_RVXF_1	500	506	PF00149	0.543
DOC_PP2B_LxvP_1	442	445	PF13499	0.456
DOC_PP2B_LxvP_1	561	564	PF13499	0.550
DOC_PP2B_PxIxI_1	390	396	PF00149	0.397
DOC_WW_Pin1_4	109	114	PF00397	0.514
DOC_WW_Pin1_4	124	129	PF00397	0.797
DOC_WW_Pin1_4	267	272	PF00397	0.606
DOC_WW_Pin1_4	325	330	PF00397	0.623
DOC_WW_Pin1_4	368	373	PF00397	0.512
DOC_WW_Pin1_4	514	519	PF00397	0.597
DOC_WW_Pin1_4	71	76	PF00397	0.619
DOC_WW_Pin1_4	86	91	PF00397	0.410
LIG_14-3-3_CanoR_1	30	35	PF00244	0.381
LIG_14-3-3_CanoR_1	53	62	PF00244	0.484
LIG_14-3-3_CanoR_1	9	15	PF00244	0.522
LIG_APCC_ABBAyCdc20_2	233	239	PF00400	0.567
LIG_BIR_III_2	125	129	PF00653	0.534
LIG_BIR_III_2	215	219	PF00653	0.628
LIG_BRCT_BRCA1_1	284	288	PF00533	0.431
LIG_BRCT_BRCA1_1	372	376	PF00533	0.533
LIG_BRCT_BRCA1_2	284	290	PF00533	0.432
LIG_EVH1_2	555	559	PF00568	0.377
LIG_FHA_1	287	293	PF00498	0.488
LIG_FHA_1	385	391	PF00498	0.431
LIG_FHA_1	438	444	PF00498	0.330
LIG_FHA_1	475	481	PF00498	0.526
LIG_FHA_1	50	56	PF00498	0.501
LIG_FHA_1	514	520	PF00498	0.511
LIG_FHA_1	558	564	PF00498	0.492
LIG_FHA_1	65	71	PF00498	0.581
LIG_FHA_1	9	15	PF00498	0.497
LIG_FHA_2	153	159	PF00498	0.379
LIG_FHA_2	245	251	PF00498	0.438
LIG_FHA_2	268	274	PF00498	0.626
LIG_FHA_2	91	97	PF00498	0.465
LIG_GBD_Chelix_1	177	185	PF00786	0.400
LIG_LIR_Gen_1	144	153	PF02991	0.443
LIG_LIR_Gen_1	273	281	PF02991	0.447
LIG_LIR_Gen_1	99	104	PF02991	0.369
LIG_LIR_Nem_3	144	150	PF02991	0.355
LIG_LIR_Nem_3	273	278	PF02991	0.505
LIG_LIR_Nem_3	404	410	PF02991	0.461
LIG_LIR_Nem_3	490	495	PF02991	0.561
LIG_LIR_Nem_3	79	85	PF02991	0.505
LIG_LIR_Nem_3	94	100	PF02991	0.389
LIG_PTAP_UEV_1	371	376	PF05743	0.446
LIG_REV1ctd_RIR_1	101	110	PF16727	0.332
LIG_SH2_CRK	147	151	PF00017	0.495
LIG_SH2_CRK	416	420	PF00017	0.501
LIG_SH2_CRK	428	432	PF00017	0.409
LIG_SH2_NCK_1	416	420	PF00017	0.454
LIG_SH2_STAP1	416	420	PF00017	0.454
LIG_SH2_STAT3	153	156	PF00017	0.324
LIG_SH2_STAT5	100	103	PF00017	0.378
LIG_SH2_STAT5	139	142	PF00017	0.329
LIG_SH2_STAT5	153	156	PF00017	0.397
LIG_SH2_STAT5	495	498	PF00017	0.543
LIG_SH3_3	268	274	PF00018	0.665
LIG_SH3_3	300	306	PF00018	0.455
LIG_SH3_3	369	375	PF00018	0.574
LIG_SH3_3	440	446	PF00018	0.433
LIG_SH3_3	512	518	PF00018	0.590
LIG_SH3_3	530	536	PF00018	0.419
LIG_SH3_3	545	551	PF00018	0.463
LIG_SH3_3	565	571	PF00018	0.483
LIG_SUMO_SIM_anti_2	336	344	PF11976	0.317
LIG_SUMO_SIM_par_1	243	250	PF11976	0.441
LIG_SUMO_SIM_par_1	516	523	PF11976	0.479
LIG_SUMO_SIM_par_1	83	89	PF11976	0.412
LIG_TRAF2_1	132	135	PF00917	0.443
LIG_TRAF2_2	314	319	PF00917	0.438
LIG_TRFH_1	275	279	PF08558	0.423
LIG_TYR_ITIM	414	419	PF00017	0.457
LIG_UBA3_1	422	427	PF00899	0.454
LIG_WW_3	217	221	PF00397	0.618
MOD_CDK_SPxxK_3	124	131	PF00069	0.584
MOD_CK1_1	265	271	PF00069	0.512
MOD_CK1_1	33	39	PF00069	0.631
MOD_CK1_1	333	339	PF00069	0.412
MOD_CK1_1	77	83	PF00069	0.339
MOD_CK2_1	244	250	PF00069	0.496
MOD_CK2_1	267	273	PF00069	0.642
MOD_CK2_1	33	39	PF00069	0.436
MOD_CK2_1	344	350	PF00069	0.446
MOD_GlcNHglycan	163	166	PF01048	0.665
MOD_GlcNHglycan	167	170	PF01048	0.616
MOD_GlcNHglycan	22	25	PF01048	0.486
MOD_GlcNHglycan	229	232	PF01048	0.610
MOD_GlcNHglycan	263	267	PF01048	0.640
MOD_GlcNHglycan	372	375	PF01048	0.608
MOD_GlcNHglycan	450	453	PF01048	0.642
MOD_GlcNHglycan	509	512	PF01048	0.299
MOD_GSK3_1	157	164	PF00069	0.618
MOD_GSK3_1	188	195	PF00069	0.509
MOD_GSK3_1	20	27	PF00069	0.450
MOD_GSK3_1	240	247	PF00069	0.422
MOD_GSK3_1	282	289	PF00069	0.481
MOD_GSK3_1	29	36	PF00069	0.670
MOD_GSK3_1	325	332	PF00069	0.616
MOD_GSK3_1	366	373	PF00069	0.514
MOD_GSK3_1	448	455	PF00069	0.631
MOD_GSK3_1	493	500	PF00069	0.575
MOD_GSK3_1	514	521	PF00069	0.512
MOD_GSK3_1	64	71	PF00069	0.726
MOD_GSK3_1	86	93	PF00069	0.472
MOD_NEK2_1	176	181	PF00069	0.552
MOD_NEK2_1	19	24	PF00069	0.446
MOD_NEK2_1	366	371	PF00069	0.564
MOD_NEK2_1	447	452	PF00069	0.444
MOD_PIKK_1	152	158	PF00454	0.373
MOD_PIKK_1	43	49	PF00454	0.643
MOD_PIKK_1	437	443	PF00454	0.330
MOD_PIKK_1	493	499	PF00454	0.543
MOD_PK_1	30	36	PF00069	0.379
MOD_PKA_1	5	11	PF00069	0.563
MOD_PKA_2	29	35	PF00069	0.384
MOD_PKA_2	455	461	PF00069	0.627
MOD_PKA_2	5	11	PF00069	0.546
MOD_PKB_1	260	268	PF00069	0.690
MOD_Plk_1	157	163	PF00069	0.400
MOD_Plk_1	329	335	PF00069	0.550
MOD_Plk_1	77	83	PF00069	0.482
MOD_Plk_4	244	250	PF00069	0.459
MOD_Plk_4	30	36	PF00069	0.442
MOD_Plk_4	554	560	PF00069	0.406
MOD_Plk_4	77	83	PF00069	0.498
MOD_Plk_4	96	102	PF00069	0.464
MOD_ProDKin_1	109	115	PF00069	0.526
MOD_ProDKin_1	124	130	PF00069	0.795
MOD_ProDKin_1	267	273	PF00069	0.596
MOD_ProDKin_1	325	331	PF00069	0.610
MOD_ProDKin_1	368	374	PF00069	0.522
MOD_ProDKin_1	514	520	PF00069	0.597
MOD_ProDKin_1	71	77	PF00069	0.607
MOD_ProDKin_1	86	92	PF00069	0.407
TRG_DiLeu_BaEn_1	273	278	PF01217	0.450
TRG_DiLeu_BaEn_2	171	177	PF01217	0.434
TRG_ENDOCYTIC_2	100	103	PF00928	0.378
TRG_ENDOCYTIC_2	147	150	PF00928	0.404
TRG_ENDOCYTIC_2	416	419	PF00928	0.505
TRG_ENDOCYTIC_2	428	431	PF00928	0.418
TRG_ER_diArg_1	108	111	PF00400	0.536
TRG_ER_diArg_1	219	222	PF00400	0.630
TRG_ER_diArg_1	252	255	PF00400	0.487
TRG_ER_diArg_1	259	262	PF00400	0.596
TRG_NLS_MonoCore_2	3	8	PF00514	0.506
TRG_NLS_MonoExtC_3	4	9	PF00514	0.504
TRG_NLS_MonoExtN_4	4	10	PF00514	0.503
TRG_Pf-PMV_PEXEL_1	364	368	PF00026	0.434
TRG_PTS2	1	38	PF00400	0.449

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PFZ8	Leptomonas seymouri	42%	100%
A0A1X0NMY7	Trypanosomatidae	29%	100%
A0A3Q8IBS6	Leishmania donovani	88%	100%
A0A422N0R9	Trypanosoma rangeli	27%	100%
A4H7A7	Leishmania braziliensis	68%	100%
A4HVQ2	Leishmania infantum	88%	100%
D0A6I9	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	28%	100%
E9APE8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	85%	100%
V5ARR9	Trypanosoma cruzi	26%	100%