Putative ras-like small GTPases

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

ras-like small GTPases, putative

Species:

Leishmania major

UniProt:

Q4QFZ8_LEIMA

TriTrypDb:

LmjF.13.1560 , LMJLV39_130020300 , LMJSD75_130020300

Length:

365

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0000323	lytic vacuole	6	2
GO:0005634	nucleus	5	2
GO:0005764	lysosome	7	2
GO:0005773	vacuole	5	2
GO:0032991	protein-containing complex	1	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	2
GO:1902494	catalytic complex	2	2
GO:1905360	GTPase complex	3	2
GO:1990131	Gtr1-Gtr2 GTPase complex	4	2

Expansion

Sequence features

Q4QFZ8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QFZ8

Function

Biological processes
Term	Name	Level	Count
GO:0001101	response to acid chemical	3	2
GO:0006950	response to stress	2	2
GO:0007154	cell communication	2	2
GO:0009267	cellular response to starvation	4	2
GO:0009605	response to external stimulus	2	2
GO:0009719	response to endogenous stimulus	2	2
GO:0009894	regulation of catabolic process	4	2
GO:0009966	regulation of signal transduction	4	2
GO:0009967	positive regulation of signal transduction	5	2
GO:0009987	cellular process	1	2
GO:0009991	response to extracellular stimulus	3	2
GO:0010033	response to organic substance	3	2
GO:0010243	response to organonitrogen compound	4	2
GO:0010506	regulation of autophagy	6	2
GO:0010646	regulation of cell communication	4	2
GO:0010647	positive regulation of cell communication	5	2
GO:0019222	regulation of metabolic process	3	2
GO:0023051	regulation of signaling	3	2
GO:0023056	positive regulation of signaling	4	2
GO:0031323	regulation of cellular metabolic process	4	2
GO:0031329	regulation of cellular catabolic process	5	2
GO:0031667	response to nutrient levels	4	2
GO:0031668	cellular response to extracellular stimulus	3	2
GO:0031669	cellular response to nutrient levels	4	2
GO:0032006	regulation of TOR signaling	6	2
GO:0032008	positive regulation of TOR signaling	7	2
GO:0033554	cellular response to stress	3	2
GO:0042221	response to chemical	2	2
GO:0042594	response to starvation	3	2
GO:0043200	response to amino acid	4	2
GO:0048518	positive regulation of biological process	3	2
GO:0048522	positive regulation of cellular process	4	2
GO:0048583	regulation of response to stimulus	3	2
GO:0048584	positive regulation of response to stimulus	4	2
GO:0050789	regulation of biological process	2	2
GO:0050794	regulation of cellular process	3	2
GO:0050896	response to stimulus	1	2
GO:0051716	cellular response to stimulus	2	2
GO:0065007	biological regulation	1	2
GO:0070887	cellular response to chemical stimulus	3	2
GO:0071229	cellular response to acid chemical	4	2
GO:0071230	cellular response to amino acid stimulus	5	2
GO:0071310	cellular response to organic substance	4	2
GO:0071417	cellular response to organonitrogen compound	4	2
GO:0071495	cellular response to endogenous stimulus	3	2
GO:0071496	cellular response to external stimulus	3	2
GO:1901698	response to nitrogen compound	3	2
GO:1901699	cellular response to nitrogen compound	4	2
GO:1901700	response to oxygen-containing compound	3	2
GO:1901701	cellular response to oxygen-containing compound	4	2
GO:1902531	regulation of intracellular signal transduction	5	2
GO:1902533	positive regulation of intracellular signal transduction	6	2

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	12
GO:0003824	catalytic activity	1	2
GO:0003924	GTPase activity	7	2
GO:0005488	binding	1	12
GO:0005525	GTP binding	5	12
GO:0016462	pyrophosphatase activity	5	2
GO:0016787	hydrolase activity	2	2
GO:0016817	hydrolase activity, acting on acid anhydrides	3	2
GO:0016818	hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	4	2
GO:0017076	purine nucleotide binding	4	12
GO:0017111	ribonucleoside triphosphate phosphatase activity	6	2
GO:0019001	guanyl nucleotide binding	5	12
GO:0032553	ribonucleotide binding	3	12
GO:0032555	purine ribonucleotide binding	4	12
GO:0032561	guanyl ribonucleotide binding	5	12
GO:0035639	purine ribonucleoside triphosphate binding	4	12
GO:0036094	small molecule binding	2	12
GO:0043167	ion binding	2	12
GO:0043168	anion binding	3	12
GO:0097159	organic cyclic compound binding	2	12
GO:0097367	carbohydrate derivative binding	2	12
GO:1901265	nucleoside phosphate binding	3	12
GO:1901363	heterocyclic compound binding	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	179	181	PF00675	0.253
CLV_NRD_NRD_1	257	259	PF00675	0.312
CLV_NRD_NRD_1	345	347	PF00675	0.476
CLV_PCSK_KEX2_1	344	346	PF00082	0.458
CLV_PCSK_SKI1_1	20	24	PF00082	0.241
CLV_PCSK_SKI1_1	223	227	PF00082	0.256
CLV_PCSK_SKI1_1	266	270	PF00082	0.359
CLV_PCSK_SKI1_1	286	290	PF00082	0.333
CLV_PCSK_SKI1_1	45	49	PF00082	0.256
CLV_PCSK_SKI1_1	65	69	PF00082	0.384
CLV_PCSK_SKI1_1	79	83	PF00082	0.317
CLV_Separin_Metazoa	228	232	PF03568	0.172
DEG_APCC_DBOX_1	222	230	PF00400	0.172
DEG_Nend_Nbox_1	1	3	PF02207	0.478
DOC_CYCLIN_RxL_1	62	73	PF00134	0.384
DOC_CYCLIN_yClb5_NLxxxL_5	65	71	PF00134	0.384
DOC_MAPK_gen_1	155	162	PF00069	0.317
DOC_MAPK_MEF2A_6	155	162	PF00069	0.317
DOC_MAPK_MEF2A_6	45	54	PF00069	0.241
DOC_MAPK_MEF2A_6	94	102	PF00069	0.241
DOC_MAPK_NFAT4_5	45	53	PF00069	0.241
DOC_PP1_RVXF_1	33	40	PF00149	0.241
DOC_USP7_MATH_1	211	215	PF00917	0.326
DOC_USP7_MATH_1	262	266	PF00917	0.278
DOC_USP7_UBL2_3	16	20	PF12436	0.521
DOC_WW_Pin1_4	150	155	PF00397	0.241
LIG_14-3-3_CanoR_1	247	251	PF00244	0.275
LIG_14-3-3_CanoR_1	277	285	PF00244	0.256
LIG_Actin_WH2_2	36	51	PF00022	0.241
LIG_APCC_ABBA_1	219	224	PF00400	0.275
LIG_APCC_ABBA_1	313	318	PF00400	0.356
LIG_BRCT_BRCA1_1	35	39	PF00533	0.241
LIG_Clathr_ClatBox_1	100	104	PF01394	0.241
LIG_eIF4E_1	95	101	PF01652	0.384
LIG_FHA_1	276	282	PF00498	0.256
LIG_FHA_1	294	300	PF00498	0.338
LIG_FHA_1	308	314	PF00498	0.209
LIG_FHA_1	318	324	PF00498	0.276
LIG_FHA_1	51	57	PF00498	0.285
LIG_FHA_1	80	86	PF00498	0.317
LIG_LIR_Apic_2	130	136	PF02991	0.278
LIG_LIR_Gen_1	248	256	PF02991	0.257
LIG_LIR_Gen_1	306	316	PF02991	0.307
LIG_LIR_LC3C_4	320	325	PF02991	0.360
LIG_LIR_Nem_3	206	212	PF02991	0.241
LIG_LIR_Nem_3	248	253	PF02991	0.257
LIG_LIR_Nem_3	306	312	PF02991	0.363
LIG_LIR_Nem_3	36	42	PF02991	0.241
LIG_LIR_Nem_3	90	96	PF02991	0.281
LIG_LYPXL_SIV_4	211	219	PF13949	0.275
LIG_PDZ_Class_3	360	365	PF00595	0.442
LIG_Pex14_2	68	72	PF04695	0.384
LIG_RPA_C_Fungi	332	344	PF08784	0.465
LIG_SH2_CRK	212	216	PF00017	0.241
LIG_SH2_GRB2like	84	87	PF00017	0.300
LIG_SH2_NCK_1	8	12	PF00017	0.413
LIG_SH2_PTP2	95	98	PF00017	0.384
LIG_SH2_SRC	6	9	PF00017	0.496
LIG_SH2_STAP1	212	216	PF00017	0.262
LIG_SH2_STAT5	141	144	PF00017	0.241
LIG_SH2_STAT5	209	212	PF00017	0.241
LIG_SH2_STAT5	42	45	PF00017	0.281
LIG_SH2_STAT5	80	83	PF00017	0.241
LIG_SH2_STAT5	84	87	PF00017	0.112
LIG_SH2_STAT5	91	94	PF00017	0.241
LIG_SH2_STAT5	95	98	PF00017	0.241
LIG_SUMO_SIM_anti_2	320	326	PF11976	0.282
LIG_SUMO_SIM_par_1	320	326	PF11976	0.282
LIG_SUMO_SIM_par_1	52	57	PF11976	0.335
LIG_TYR_ITIM	210	215	PF00017	0.275
LIG_UBA3_1	161	170	PF00899	0.294
MOD_CDK_SPK_2	150	155	PF00069	0.317
MOD_CDK_SPxxK_3	150	157	PF00069	0.241
MOD_CK1_1	127	133	PF00069	0.403
MOD_CK1_1	202	208	PF00069	0.283
MOD_CK1_1	303	309	PF00069	0.450
MOD_GlcNHglycan	127	130	PF01048	0.370
MOD_GSK3_1	123	130	PF00069	0.438
MOD_GSK3_1	198	205	PF00069	0.288
MOD_GSK3_1	211	218	PF00069	0.267
MOD_GSK3_1	275	282	PF00069	0.305
MOD_GSK3_1	29	36	PF00069	0.245
MOD_GSK3_1	303	310	PF00069	0.354
MOD_GSK3_1	350	357	PF00069	0.416
MOD_GSK3_1	52	59	PF00069	0.295
MOD_N-GLC_1	293	298	PF02516	0.378
MOD_NEK2_1	198	203	PF00069	0.241
MOD_NEK2_1	210	215	PF00069	0.241
MOD_NEK2_1	251	256	PF00069	0.312
MOD_NEK2_1	288	293	PF00069	0.361
MOD_NEK2_1	50	55	PF00069	0.242
MOD_NEK2_1	56	61	PF00069	0.243
MOD_NEK2_2	204	209	PF00069	0.269
MOD_PIKK_1	56	62	PF00454	0.275
MOD_PKA_2	109	115	PF00069	0.305
MOD_PKA_2	246	252	PF00069	0.241
MOD_PKA_2	276	282	PF00069	0.255
MOD_Plk_1	245	251	PF00069	0.275
MOD_Plk_1	294	300	PF00069	0.370
MOD_Plk_1	317	323	PF00069	0.312
MOD_Plk_1	56	62	PF00069	0.256
MOD_Plk_4	199	205	PF00069	0.241
MOD_Plk_4	211	217	PF00069	0.241
MOD_Plk_4	246	252	PF00069	0.241
MOD_ProDKin_1	150	156	PF00069	0.241
MOD_SUMO_rev_2	12	18	PF00179	0.566
MOD_SUMO_rev_2	126	136	PF00179	0.345
MOD_SUMO_rev_2	326	331	PF00179	0.471
TRG_DiLeu_BaEn_2	143	149	PF01217	0.317
TRG_DiLeu_BaEn_2	245	251	PF01217	0.256
TRG_ENDOCYTIC_2	212	215	PF00928	0.241
TRG_ENDOCYTIC_2	95	98	PF00928	0.384
TRG_ER_diArg_1	344	346	PF00400	0.458
TRG_NES_CRM1_1	90	104	PF08389	0.241
TRG_Pf-PMV_PEXEL_1	147	151	PF00026	0.335

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PEZ9	Leptomonas seymouri	83%	100%
A0A0S4INT4	Bodo saltans	45%	97%
A0A1X0NJF6	Trypanosomatidae	67%	100%
A0A3R7MBW5	Trypanosoma rangeli	65%	99%
A0A3S7WSQ4	Leishmania donovani	97%	100%
A4H7E6	Leishmania braziliensis	91%	100%
A4HVT5	Leishmania infantum	97%	100%
D0A6F6	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	56%	100%
E9API0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	96%	100%
O74824	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	35%	100%
Q00582	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	34%	100%
Q3SX43	Bos taurus	36%	100%
Q54IK1	Dictyostelium discoideum	36%	100%
Q5VZM2	Homo sapiens	33%	98%
Q63486	Rattus norvegicus	36%	100%
Q63487	Rattus norvegicus	33%	98%
Q6NTA4	Mus musculus	33%	98%
Q7L523	Homo sapiens	36%	100%
Q80X95	Mus musculus	36%	100%
V5BM39	Trypanosoma cruzi	65%	100%