Ubiquitin-conjugating enzyme-like protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

ubiquitin-conjugating enzyme, putative

Species:

Leishmania major

UniProt:

Q4QFZ6_LEIMA

TriTrypDb:

LmjF.13.1580 , LMJLV39_130020500 , LMJSD75_130020500

Length:

138

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 6
Silverman et al.	yes	yes: 3
Pissara et al.	yes	yes: 15

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	yes	yes: 5

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 10

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

Q4QFZ6

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QFZ6

PDB structure(s): 6zm3_BBB , 6zm3_DDD

Function

Biological processes
Term	Name	Level	Count
GO:0000209	protein polyubiquitination	8	2
GO:0006139	nucleobase-containing compound metabolic process	3	2
GO:0006259	DNA metabolic process	4	2
GO:0006281	DNA repair	5	2
GO:0006301	postreplication repair	6	2
GO:0006725	cellular aromatic compound metabolic process	3	2
GO:0006807	nitrogen compound metabolic process	2	2
GO:0006950	response to stress	2	2
GO:0006974	DNA damage response	4	2
GO:0008152	metabolic process	1	2
GO:0009987	cellular process	1	2
GO:0016567	protein ubiquitination	7	2
GO:0019538	protein metabolic process	3	2
GO:0032446	protein modification by small protein conjugation	6	2
GO:0033554	cellular response to stress	3	2
GO:0034641	cellular nitrogen compound metabolic process	3	2
GO:0036211	protein modification process	4	2
GO:0043170	macromolecule metabolic process	3	2
GO:0043412	macromolecule modification	4	2
GO:0044237	cellular metabolic process	2	2
GO:0044238	primary metabolic process	2	2
GO:0044260	obsolete cellular macromolecule metabolic process	3	2
GO:0046483	heterocycle metabolic process	3	2
GO:0050896	response to stimulus	1	2
GO:0051716	cellular response to stimulus	2	2
GO:0070534	protein K63-linked ubiquitination	9	2
GO:0070647	protein modification by small protein conjugation or removal	5	2
GO:0071704	organic substance metabolic process	2	2
GO:0090304	nucleic acid metabolic process	4	2
GO:1901360	organic cyclic compound metabolic process	3	2
GO:1901564	organonitrogen compound metabolic process	3	2

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	6
GO:0016874	ligase activity	2	6

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	106	108	PF00675	0.400
CLV_NRD_NRD_1	117	119	PF00675	0.332
CLV_NRD_NRD_1	126	128	PF00675	0.217
CLV_PCSK_KEX2_1	106	108	PF00082	0.409
CLV_PCSK_PC7_1	102	108	PF00082	0.419
CLV_Separin_Metazoa	3	7	PF03568	0.459
DEG_MDM2_SWIB_1	38	46	PF02201	0.424
DOC_MAPK_gen_1	106	114	PF00069	0.488
DOC_MAPK_MEF2A_6	71	78	PF00069	0.405
DOC_SPAK_OSR1_1	97	101	PF12202	0.419
DOC_USP7_MATH_1	133	137	PF00917	0.644
LIG_14-3-3_CanoR_1	106	111	PF00244	0.386
LIG_14-3-3_CanoR_1	57	62	PF00244	0.386
LIG_APCC_ABBA_1	61	66	PF00400	0.370
LIG_BH_BH3_1	23	39	PF00452	0.424
LIG_BRCT_BRCA1_1	50	54	PF00533	0.488
LIG_FHA_1	32	38	PF00498	0.405
LIG_FHA_2	106	112	PF00498	0.514
LIG_FHA_2	50	56	PF00498	0.347
LIG_LIR_Gen_1	96	105	PF02991	0.408
LIG_LIR_Nem_3	36	41	PF02991	0.477
LIG_LIR_Nem_3	55	59	PF02991	0.227
LIG_LIR_Nem_3	66	72	PF02991	0.505
LIG_LIR_Nem_3	96	101	PF02991	0.408
LIG_LYPXL_yS_3	69	72	PF13949	0.505
LIG_Pex14_2	38	42	PF04695	0.424
LIG_Rb_LxCxE_1	60	80	PF01857	0.465
LIG_SH2_CRK	56	60	PF00017	0.505
LIG_SH2_GRB2like	41	44	PF00017	0.430
LIG_SH2_STAT5	64	67	PF00017	0.405
LIG_SH3_3	127	133	PF00018	0.490
LIG_SH3_3	44	50	PF00018	0.462
LIG_SH3_3	67	73	PF00018	0.381
LIG_SH3_3	79	85	PF00018	0.370
LIG_SUMO_SIM_par_1	57	62	PF11976	0.430
LIG_UBA3_1	10	19	PF00899	0.370
MOD_CK2_1	105	111	PF00069	0.513
MOD_CK2_1	49	55	PF00069	0.370
MOD_LATS_1	75	81	PF00433	0.405
MOD_N-GLC_1	25	30	PF02516	0.399
MOD_N-GLC_1	42	47	PF02516	0.291
MOD_NEK2_2	133	138	PF00069	0.630
MOD_PKA_1	106	112	PF00069	0.430
MOD_PKA_2	105	111	PF00069	0.409
MOD_PKA_2	31	37	PF00069	0.462
MOD_Plk_1	25	31	PF00069	0.407
MOD_Plk_4	42	48	PF00069	0.370
TRG_DiLeu_BaLyEn_6	6	11	PF01217	0.447
TRG_ENDOCYTIC_2	56	59	PF00928	0.505
TRG_ENDOCYTIC_2	69	72	PF00928	0.505
TRG_Pf-PMV_PEXEL_1	127	131	PF00026	0.496
TRG_Pf-PMV_PEXEL_1	57	62	PF00026	0.405
TRG_Pf-PMV_PEXEL_1	9	13	PF00026	0.370

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IJW9	Leptomonas seymouri	80%	100%
A0A0S4INP2	Bodo saltans	70%	72%
A0A1X0NJQ5	Trypanosomatidae	72%	100%
A0A3S5H6Q3	Leishmania donovani	99%	100%
A0A422P4A8	Trypanosoma rangeli	75%	100%
A4H7E8	Leishmania braziliensis	91%	100%
A4HVS0	Leishmania infantum	99%	100%
D0A6F3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	67%	100%
E9API2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	95%	100%
O45495	Caenorhabditis elegans	44%	99%
O74983	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	52%	99%
P25153	Drosophila melanogaster	28%	91%
P53152	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	55%	100%
Q13404	Homo sapiens	46%	94%
Q15819	Homo sapiens	45%	95%
Q3SZ43	Bos taurus	45%	95%
Q3SZ52	Bos taurus	46%	94%
Q54D06	Dictyostelium discoideum	46%	100%
Q5F3Z3	Gallus gallus	45%	96%
Q5R4Z6	Pongo abelii	46%	94%
Q5R6C9	Pongo abelii	45%	95%
Q6PEH5	Danio rerio	43%	95%
Q7M767	Rattus norvegicus	45%	95%
Q7ZYP0	Xenopus laevis	45%	95%
Q90879	Gallus gallus	46%	95%
Q93YP0	Arabidopsis thaliana	51%	87%
Q9CAB6	Arabidopsis thaliana	51%	87%
Q9CZY3	Mus musculus	46%	94%
Q9D2M8	Mus musculus	45%	95%
Q9SJ44	Arabidopsis thaliana	53%	95%
Q9SVD7	Arabidopsis thaliana	51%	95%
V5BRP1	Trypanosoma cruzi	72%	100%