Putative exosome complex exonuclease

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

exosome complex exonuclease, putative

Species:

Leishmania major

UniProt:

Q4QFY3_LEIMA

TriTrypDb:

LmjF.14.0010 , LMJLV39_140005000 , LMJSD75_140005000

Length:

307

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 1, no: 9
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0000176	nuclear exosome (RNase complex)	3	2
GO:0000177	cytoplasmic exosome (RNase complex)	5	2
GO:0000178	exosome (RNase complex)	4	11
GO:0032991	protein-containing complex	1	11
GO:0140513	nuclear protein-containing complex	2	2
GO:1902494	catalytic complex	2	11
GO:1905354	exoribonuclease complex	3	11

Expansion

Sequence features

Q4QFY3

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QFY3

Function

Biological processes
Term	Name	Level	Count
GO:0000291	nuclear-transcribed mRNA catabolic process, exonucleolytic	8	2
GO:0000459	exonucleolytic trimming involved in rRNA processing	8	2
GO:0000467	exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)	9	2
GO:0000469	cleavage involved in rRNA processing	7	2
GO:0000956	nuclear-transcribed mRNA catabolic process	7	2
GO:0006139	nucleobase-containing compound metabolic process	3	2
GO:0006364	rRNA processing	8	2
GO:0006396	RNA processing	6	2
GO:0006399	tRNA metabolic process	7	2
GO:0006401	RNA catabolic process	5	2
GO:0006402	mRNA catabolic process	6	2
GO:0006725	cellular aromatic compound metabolic process	3	2
GO:0006807	nitrogen compound metabolic process	2	2
GO:0008152	metabolic process	1	2
GO:0009056	catabolic process	2	2
GO:0009057	macromolecule catabolic process	4	2
GO:0009892	negative regulation of metabolic process	4	2
GO:0009987	cellular process	1	2
GO:0010468	regulation of gene expression	5	2
GO:0010605	negative regulation of macromolecule metabolic process	5	2
GO:0010629	negative regulation of gene expression	6	2
GO:0016070	RNA metabolic process	5	2
GO:0016071	mRNA metabolic process	6	2
GO:0016072	rRNA metabolic process	7	2
GO:0016073	snRNA metabolic process	7	2
GO:0016074	sno(s)RNA metabolic process	7	2
GO:0016075	rRNA catabolic process	7	2
GO:0016078	tRNA catabolic process	7	2
GO:0016180	snRNA processing	8	2
GO:0019222	regulation of metabolic process	3	2
GO:0019439	aromatic compound catabolic process	4	2
GO:0031123	RNA 3'-end processing	7	2
GO:0031125	rRNA 3'-end processing	9	2
GO:0031126	sno(s)RNA 3'-end processing	9	2
GO:0034427	nuclear-transcribed mRNA catabolic process, exonucleolytic, 3'-5'	9	2
GO:0034470	ncRNA processing	7	2
GO:0034472	snRNA 3'-end processing	8	2
GO:0034475	U4 snRNA 3'-end processing	9	2
GO:0034641	cellular nitrogen compound metabolic process	3	2
GO:0034655	nucleobase-containing compound catabolic process	4	2
GO:0034660	ncRNA metabolic process	6	2
GO:0034661	ncRNA catabolic process	6	2
GO:0043144	sno(s)RNA processing	8	2
GO:0043170	macromolecule metabolic process	3	2
GO:0043628	regulatory ncRNA 3'-end processing	8	2
GO:0043632	modification-dependent macromolecule catabolic process	5	2
GO:0043633	polyadenylation-dependent RNA catabolic process	6	2
GO:0043634	polyadenylation-dependent ncRNA catabolic process	7	2
GO:0043928	exonucleolytic catabolism of deadenylated mRNA	9	2
GO:0044237	cellular metabolic process	2	2
GO:0044238	primary metabolic process	2	2
GO:0044248	cellular catabolic process	3	2
GO:0044260	obsolete cellular macromolecule metabolic process	3	2
GO:0044265	obsolete cellular macromolecule catabolic process	4	2
GO:0044270	cellular nitrogen compound catabolic process	4	2
GO:0046483	heterocycle metabolic process	3	2
GO:0046700	heterocycle catabolic process	4	2
GO:0048519	negative regulation of biological process	3	2
GO:0050789	regulation of biological process	2	2
GO:0060255	regulation of macromolecule metabolic process	4	2
GO:0065007	biological regulation	1	2
GO:0071025	RNA surveillance	6	2
GO:0071027	nuclear RNA surveillance	7	2
GO:0071029	nuclear ncRNA surveillance	7	2
GO:0071034	CUT catabolic process	7	2
GO:0071035	nuclear polyadenylation-dependent rRNA catabolic process	8	2
GO:0071038	nuclear polyadenylation-dependent tRNA catabolic process	8	2
GO:0071043	CUT metabolic process	7	2
GO:0071046	nuclear polyadenylation-dependent ncRNA catabolic process	8	2
GO:0071051	polyadenylation-dependent snoRNA 3'-end processing	10	2
GO:0071704	organic substance metabolic process	2	2
GO:0090304	nucleic acid metabolic process	4	2
GO:0090305	nucleic acid phosphodiester bond hydrolysis	5	2
GO:0090501	RNA phosphodiester bond hydrolysis	6	2
GO:0090503	RNA phosphodiester bond hydrolysis, exonucleolytic	7	2
GO:0106354	tRNA surveillance	7	2
GO:1901360	organic cyclic compound metabolic process	3	2
GO:1901361	organic cyclic compound catabolic process	4	2
GO:1901575	organic substance catabolic process	3	2

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	11
GO:0003723	RNA binding	4	11
GO:0003824	catalytic activity	1	10
GO:0004518	nuclease activity	4	9
GO:0004527	exonuclease activity	5	9
GO:0005488	binding	1	11
GO:0016787	hydrolase activity	2	10
GO:0016788	hydrolase activity, acting on ester bonds	3	9
GO:0097159	organic cyclic compound binding	2	11
GO:1901363	heterocyclic compound binding	2	11

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	216	220	PF00656	0.521
CLV_NRD_NRD_1	124	126	PF00675	0.444
CLV_NRD_NRD_1	294	296	PF00675	0.536
CLV_PCSK_KEX2_1	123	125	PF00082	0.448
CLV_PCSK_KEX2_1	184	186	PF00082	0.213
CLV_PCSK_KEX2_1	294	296	PF00082	0.531
CLV_PCSK_PC1ET2_1	184	186	PF00082	0.226
CLV_PCSK_SKI1_1	134	138	PF00082	0.389
CLV_PCSK_SKI1_1	184	188	PF00082	0.226
CLV_PCSK_SKI1_1	278	282	PF00082	0.436
CLV_PCSK_SKI1_1	44	48	PF00082	0.347
CLV_PCSK_SKI1_1	72	76	PF00082	0.411
DOC_ANK_TNKS_1	148	155	PF00023	0.402
DOC_MAPK_gen_1	165	174	PF00069	0.298
DOC_MAPK_gen_1	184	193	PF00069	0.420
DOC_MAPK_MEF2A_6	168	176	PF00069	0.334
DOC_MAPK_RevD_3	110	125	PF00069	0.437
DOC_PP1_SILK_1	119	124	PF00149	0.479
DOC_USP7_MATH_1	215	219	PF00917	0.594
DOC_WW_Pin1_4	148	153	PF00397	0.407
LIG_14-3-3_CanoR_1	31	37	PF00244	0.363
LIG_14-3-3_CanoR_1	67	72	PF00244	0.350
LIG_BIR_II_1	1	5	PF00653	0.483
LIG_FHA_1	196	202	PF00498	0.525
LIG_FHA_1	63	69	PF00498	0.350
LIG_FHA_2	21	27	PF00498	0.553
LIG_GBD_Chelix_1	259	267	PF00786	0.415
LIG_Integrin_RGD_1	127	129	PF01839	0.543
LIG_LIR_Nem_3	164	169	PF02991	0.387
LIG_PCNA_yPIPBox_3	72	86	PF02747	0.472
LIG_PDZ_Class_1	302	307	PF00595	0.511
LIG_SH2_CRK	166	170	PF00017	0.351
LIG_SH2_STAP1	196	200	PF00017	0.381
LIG_SH2_STAT3	196	199	PF00017	0.459
LIG_SH2_STAT5	29	32	PF00017	0.415
LIG_SH3_3	112	118	PF00018	0.374
LIG_SH3_3	269	275	PF00018	0.466
LIG_SUMO_SIM_anti_2	107	114	PF11976	0.344
LIG_SUMO_SIM_anti_2	2	11	PF11976	0.435
LIG_SUMO_SIM_par_1	2	11	PF11976	0.477
LIG_SUMO_SIM_par_1	89	94	PF11976	0.455
LIG_WRC_WIRS_1	33	38	PF05994	0.435
MOD_CK1_1	104	110	PF00069	0.367
MOD_CK1_1	113	119	PF00069	0.370
MOD_CK1_1	157	163	PF00069	0.355
MOD_CK2_1	111	117	PF00069	0.455
MOD_CK2_1	21	27	PF00069	0.497
MOD_CK2_1	215	221	PF00069	0.613
MOD_GlcNHglycan	169	172	PF01048	0.388
MOD_GlcNHglycan	58	61	PF01048	0.447
MOD_GlcNHglycan	92	96	PF01048	0.444
MOD_GSK3_1	174	181	PF00069	0.541
MOD_GSK3_1	209	216	PF00069	0.567
MOD_GSK3_1	220	227	PF00069	0.465
MOD_GSK3_1	251	258	PF00069	0.466
MOD_N-GLC_1	102	107	PF02516	0.417
MOD_NEK2_1	1	6	PF00069	0.407
MOD_NEK2_1	110	115	PF00069	0.358
MOD_NEK2_1	270	275	PF00069	0.475
MOD_NEK2_2	154	159	PF00069	0.324
MOD_NEK2_2	265	270	PF00069	0.335
MOD_PIKK_1	102	108	PF00454	0.388
MOD_PIKK_1	195	201	PF00454	0.490
MOD_PK_1	67	73	PF00069	0.350
MOD_PKA_1	294	300	PF00069	0.468
MOD_PKA_2	167	173	PF00069	0.426
MOD_PKA_2	178	184	PF00069	0.486
MOD_PKA_2	294	300	PF00069	0.521
MOD_Plk_1	255	261	PF00069	0.329
MOD_Plk_1	270	276	PF00069	0.377
MOD_Plk_4	1	7	PF00069	0.404
MOD_Plk_4	178	184	PF00069	0.456
MOD_Plk_4	265	271	PF00069	0.472
MOD_ProDKin_1	148	154	PF00069	0.406
MOD_SUMO_rev_2	16	22	PF00179	0.476
TRG_ENDOCYTIC_2	166	169	PF00928	0.336
TRG_ER_diArg_1	122	125	PF00400	0.457
TRG_ER_diArg_1	294	296	PF00400	0.654
TRG_NES_CRM1_1	279	292	PF08389	0.393
TRG_Pf-PMV_PEXEL_1	134	138	PF00026	0.371
TRG_Pf-PMV_PEXEL_1	44	49	PF00026	0.356

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I616	Leptomonas seymouri	77%	100%
A0A0S4ISJ0	Bodo saltans	42%	79%
A0A1X0NQG9	Trypanosomatidae	54%	100%
A0A3Q8IBV3	Leishmania donovani	92%	100%
A0A422NXH9	Trypanosoma rangeli	53%	100%
A4H7G3	Leishmania braziliensis	82%	100%
A4HVU3	Leishmania infantum	92%	100%
A5UJS0	Methanobrevibacter smithii (strain ATCC 35061 / DSM 861 / OCM 144 / PS)	24%	100%
C9ZT82	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	49%	95%
E3GZ90	Methanothermus fervidus (strain ATCC 43054 / DSM 2088 / JCM 10308 / V24 S)	25%	100%
E9APJ5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	90%	100%
O59221	Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)	24%	100%
P38792	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	33%	86%
Q09704	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	34%	93%
Q13868	Homo sapiens	35%	100%
Q2KID0	Bos taurus	35%	100%
Q6L222	Picrophilus torridus (strain ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828)	23%	100%
Q8VBV3	Mus musculus	38%	100%
Q9V120	Pyrococcus abyssi (strain GE5 / Orsay)	24%	100%
Q9ZVT7	Arabidopsis thaliana	33%	95%