SpoU_methylase domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

hypothetical protein, conserved

Species:

Leishmania major

UniProt:

Q4QFS5_LEIMA

TriTrypDb:

LmjF.14.0600 , LMJLV39_140011900 , LMJSD75_140011500

Length:

488

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 7
NetGPI	no	yes: 0, no: 7

Cellular components
Term	Name	Level	Count
GO:0005829	cytosol	2	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

Q4QFS5

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QFS5

Function

Biological processes
Term	Name	Level	Count
GO:0001510	RNA methylation	4	2
GO:0002128	tRNA nucleoside ribose methylation	6	2
GO:0006139	nucleobase-containing compound metabolic process	3	8
GO:0006396	RNA processing	6	8
GO:0006399	tRNA metabolic process	7	2
GO:0006400	tRNA modification	6	2
GO:0006725	cellular aromatic compound metabolic process	3	8
GO:0006807	nitrogen compound metabolic process	2	8
GO:0008033	tRNA processing	8	2
GO:0008152	metabolic process	1	8
GO:0009451	RNA modification	5	2
GO:0009987	cellular process	1	8
GO:0016070	RNA metabolic process	5	8
GO:0030488	tRNA methylation	5	2
GO:0032259	methylation	2	2
GO:0034470	ncRNA processing	7	2
GO:0034641	cellular nitrogen compound metabolic process	3	8
GO:0034660	ncRNA metabolic process	6	2
GO:0043170	macromolecule metabolic process	3	8
GO:0043412	macromolecule modification	4	2
GO:0043414	macromolecule methylation	3	2
GO:0044237	cellular metabolic process	2	8
GO:0044238	primary metabolic process	2	8
GO:0044260	obsolete cellular macromolecule metabolic process	3	2
GO:0046483	heterocycle metabolic process	3	8
GO:0071704	organic substance metabolic process	2	8
GO:0090304	nucleic acid metabolic process	4	8
GO:1901360	organic cyclic compound metabolic process	3	8

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	8
GO:0008168	methyltransferase activity	4	8
GO:0008171	O-methyltransferase activity	5	2
GO:0008173	RNA methyltransferase activity	4	8
GO:0008175	tRNA methyltransferase activity	5	2
GO:0008757	S-adenosylmethionine-dependent methyltransferase activity	5	2
GO:0016300	tRNA (uracil) methyltransferase activity	6	2
GO:0016427	tRNA (cytosine) methyltransferase activity	6	2
GO:0016740	transferase activity	2	8
GO:0016741	transferase activity, transferring one-carbon groups	3	8
GO:0052665	tRNA (uracil-2'-O-)-methyltransferase activity	7	2
GO:0052666	tRNA (cytosine-2'-O-)-methyltransferase activity	7	2
GO:0062105	RNA 2'-O-methyltransferase activity	5	2
GO:0106050	tRNA 2'-O-methyltransferase activity	6	2
GO:0140098	catalytic activity, acting on RNA	3	8
GO:0140101	catalytic activity, acting on a tRNA	4	2
GO:0140640	catalytic activity, acting on a nucleic acid	2	8
GO:0003676	nucleic acid binding	3	1
GO:0003723	RNA binding	4	1
GO:0005488	binding	1	1
GO:0097159	organic cyclic compound binding	2	1
GO:1901363	heterocyclic compound binding	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_MEL_PAP_1	282	288	PF00089	0.463
CLV_NRD_NRD_1	190	192	PF00675	0.467
CLV_NRD_NRD_1	194	196	PF00675	0.441
CLV_NRD_NRD_1	61	63	PF00675	0.611
CLV_NRD_NRD_1	76	78	PF00675	0.646
CLV_PCSK_FUR_1	74	78	PF00082	0.541
CLV_PCSK_KEX2_1	144	146	PF00082	0.709
CLV_PCSK_KEX2_1	190	192	PF00082	0.467
CLV_PCSK_KEX2_1	194	196	PF00082	0.441
CLV_PCSK_KEX2_1	76	78	PF00082	0.531
CLV_PCSK_PC1ET2_1	144	146	PF00082	0.709
CLV_PCSK_PC7_1	190	196	PF00082	0.343
CLV_PCSK_PC7_1	72	78	PF00082	0.515
CLV_PCSK_SKI1_1	190	194	PF00082	0.491
CLV_PCSK_SKI1_1	469	473	PF00082	0.517
DEG_SPOP_SBC_1	47	51	PF00917	0.662
DOC_CKS1_1	221	226	PF01111	0.392
DOC_CYCLIN_yCln2_LP_2	239	245	PF00134	0.509
DOC_CYCLIN_yCln2_LP_2	82	88	PF00134	0.535
DOC_MAPK_gen_1	74	82	PF00069	0.501
DOC_MAPK_MEF2A_6	318	325	PF00069	0.447
DOC_PP1_SILK_1	228	233	PF00149	0.377
DOC_PP2B_LxvP_1	323	326	PF13499	0.426
DOC_PP2B_LxvP_1	82	85	PF13499	0.452
DOC_PP4_FxxP_1	375	378	PF00568	0.495
DOC_USP7_MATH_1	21	25	PF00917	0.814
DOC_USP7_MATH_1	274	278	PF00917	0.580
DOC_USP7_MATH_1	349	353	PF00917	0.574
DOC_USP7_MATH_1	47	51	PF00917	0.657
DOC_WW_Pin1_4	206	211	PF00397	0.612
DOC_WW_Pin1_4	220	225	PF00397	0.333
DOC_WW_Pin1_4	23	28	PF00397	0.674
DOC_WW_Pin1_4	30	35	PF00397	0.591
DOC_WW_Pin1_4	345	350	PF00397	0.725
DOC_WW_Pin1_4	43	48	PF00397	0.552
DOC_WW_Pin1_4	483	488	PF00397	0.563
LIG_14-3-3_CanoR_1	156	166	PF00244	0.693
LIG_FHA_1	109	115	PF00498	0.489
LIG_FHA_1	251	257	PF00498	0.654
LIG_FHA_1	377	383	PF00498	0.712
LIG_FHA_1	38	44	PF00498	0.623
LIG_FHA_1	390	396	PF00498	0.691
LIG_FHA_1	436	442	PF00498	0.417
LIG_FHA_1	49	55	PF00498	0.534
LIG_FHA_1	98	104	PF00498	0.533
LIG_FHA_2	158	164	PF00498	0.697
LIG_FHA_2	182	188	PF00498	0.560
LIG_LIR_Gen_1	125	132	PF02991	0.508
LIG_LIR_Gen_1	329	339	PF02991	0.388
LIG_LIR_Nem_3	125	131	PF02991	0.497
LIG_LIR_Nem_3	329	335	PF02991	0.364
LIG_LIR_Nem_3	466	471	PF02991	0.488
LIG_NRBOX	188	194	PF00104	0.408
LIG_Pex14_1	468	472	PF04695	0.504
LIG_SH2_CRK	444	448	PF00017	0.425
LIG_SH2_NCK_1	371	375	PF00017	0.455
LIG_SH2_SRC	305	308	PF00017	0.457
LIG_SH2_SRC	371	374	PF00017	0.451
LIG_SH2_STAP1	128	132	PF00017	0.505
LIG_SH2_STAP1	305	309	PF00017	0.515
LIG_SH2_STAT3	339	342	PF00017	0.463
LIG_SH2_STAT5	403	406	PF00017	0.371
LIG_SH3_3	215	221	PF00018	0.405
LIG_SH3_3	343	349	PF00018	0.598
LIG_SH3_3	379	385	PF00018	0.501
LIG_SH3_3	422	428	PF00018	0.369
LIG_SUMO_SIM_par_1	355	360	PF11976	0.360
LIG_TRAF2_1	159	162	PF00917	0.632
LIG_TRAF2_1	252	255	PF00917	0.620
LIG_TYR_ITIM	442	447	PF00017	0.411
LIG_WRC_WIRS_1	286	291	PF05994	0.427
MOD_CK1_1	108	114	PF00069	0.549
MOD_CK1_1	140	146	PF00069	0.622
MOD_CK1_1	23	29	PF00069	0.711
MOD_CK1_1	350	356	PF00069	0.530
MOD_CK1_1	376	382	PF00069	0.698
MOD_CK1_1	46	52	PF00069	0.660
MOD_CK1_1	463	469	PF00069	0.574
MOD_CK2_1	157	163	PF00069	0.648
MOD_CK2_1	181	187	PF00069	0.457
MOD_CK2_1	249	255	PF00069	0.710
MOD_DYRK1A_RPxSP_1	347	351	PF00069	0.546
MOD_GlcNHglycan	107	110	PF01048	0.567
MOD_GlcNHglycan	11	14	PF01048	0.638
MOD_GlcNHglycan	276	279	PF01048	0.647
MOD_GlcNHglycan	291	294	PF01048	0.357
MOD_GlcNHglycan	412	415	PF01048	0.433
MOD_GSK3_1	104	111	PF00069	0.542
MOD_GSK3_1	133	140	PF00069	0.626
MOD_GSK3_1	157	164	PF00069	0.699
MOD_GSK3_1	245	252	PF00069	0.647
MOD_GSK3_1	259	266	PF00069	0.635
MOD_GSK3_1	274	281	PF00069	0.741
MOD_GSK3_1	285	292	PF00069	0.508
MOD_GSK3_1	345	352	PF00069	0.581
MOD_GSK3_1	43	50	PF00069	0.653
MOD_N-GLC_1	206	211	PF02516	0.662
MOD_NEK2_1	137	142	PF00069	0.671
MOD_NEK2_1	259	264	PF00069	0.607
MOD_NEK2_1	289	294	PF00069	0.366
MOD_NEK2_1	435	440	PF00069	0.415
MOD_NEK2_1	481	486	PF00069	0.502
MOD_NEK2_2	122	127	PF00069	0.492
MOD_NEK2_2	278	283	PF00069	0.528
MOD_PIKK_1	137	143	PF00454	0.690
MOD_PIKK_1	169	175	PF00454	0.624
MOD_PIKK_1	376	382	PF00454	0.698
MOD_PKA_2	157	163	PF00069	0.698
MOD_PKA_2	169	175	PF00069	0.549
MOD_PKA_2	445	451	PF00069	0.359
MOD_PKA_2	481	487	PF00069	0.591
MOD_PKB_1	283	291	PF00069	0.453
MOD_Plk_1	133	139	PF00069	0.559
MOD_Plk_1	161	167	PF00069	0.636
MOD_Plk_4	161	167	PF00069	0.697
MOD_Plk_4	285	291	PF00069	0.430
MOD_ProDKin_1	206	212	PF00069	0.594
MOD_ProDKin_1	220	226	PF00069	0.329
MOD_ProDKin_1	23	29	PF00069	0.675
MOD_ProDKin_1	30	36	PF00069	0.591
MOD_ProDKin_1	345	351	PF00069	0.720
MOD_ProDKin_1	43	49	PF00069	0.554
TRG_DiLeu_BaEn_4	254	260	PF01217	0.511
TRG_DiLeu_BaLyEn_6	188	193	PF01217	0.456
TRG_DiLeu_BaLyEn_6	209	214	PF01217	0.486
TRG_ENDOCYTIC_2	128	131	PF00928	0.513
TRG_ENDOCYTIC_2	444	447	PF00928	0.422
TRG_ENDOCYTIC_2	95	98	PF00928	0.538
TRG_ER_diArg_1	189	191	PF00400	0.462
TRG_ER_diArg_1	193	195	PF00400	0.438
TRG_ER_diArg_1	282	285	PF00400	0.504
TRG_ER_diArg_1	480	483	PF00400	0.537
TRG_ER_diArg_1	73	76	PF00400	0.657
TRG_Pf-PMV_PEXEL_1	130	134	PF00026	0.553
TRG_Pf-PMV_PEXEL_1	318	322	PF00026	0.490

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IIC5	Leptomonas seymouri	50%	94%
A0A3S7WSX9	Leishmania donovani	93%	100%
A4H7L7	Leishmania braziliensis	69%	93%
A4HW02	Leishmania infantum	93%	100%
E9APQ4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	89%	96%
V5BIX5	Trypanosoma cruzi	31%	100%