Glutathione synthetase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

glutathione synthetase, putative

Species:

Leishmania major

UniProt:

Q4QFP4_LEIMA

TriTrypDb:

LmjF.14.0910 , LMJLV39_140015800 , LMJSD75_140015200

Length:

606

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005730	nucleolus	5	2
GO:0005737	cytoplasm	2	2
GO:0005829	cytosol	2	2
GO:0043226	organelle	2	2
GO:0043228	non-membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043232	intracellular non-membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	3
GO:0016020	membrane	2	1

Expansion

Sequence features

Q4QFP4

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QFP4

Function

Could not find GO biological_process term for this entry.

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	12
GO:0000287	magnesium ion binding	5	12
GO:0003824	catalytic activity	1	12
GO:0004363	glutathione synthase activity	3	12
GO:0005488	binding	1	12
GO:0005524	ATP binding	5	12
GO:0016874	ligase activity	2	12
GO:0016879	ligase activity, forming carbon-nitrogen bonds	3	12
GO:0016881	acid-amino acid ligase activity	4	12
GO:0017076	purine nucleotide binding	4	12
GO:0030554	adenyl nucleotide binding	5	12
GO:0032553	ribonucleotide binding	3	12
GO:0032555	purine ribonucleotide binding	4	12
GO:0032559	adenyl ribonucleotide binding	5	12
GO:0033218	amide binding	2	12
GO:0035639	purine ribonucleoside triphosphate binding	4	12
GO:0036094	small molecule binding	2	12
GO:0042277	peptide binding	3	12
GO:0043167	ion binding	2	12
GO:0043168	anion binding	3	12
GO:0043169	cation binding	3	12
GO:0043295	glutathione binding	3	12
GO:0046872	metal ion binding	4	12
GO:0072341	modified amino acid binding	2	12
GO:0097159	organic cyclic compound binding	2	12
GO:0097367	carbohydrate derivative binding	2	12
GO:1900750	oligopeptide binding	4	12
GO:1901265	nucleoside phosphate binding	3	12
GO:1901363	heterocyclic compound binding	2	12
GO:1901681	sulfur compound binding	2	12
GO:1904091	non-ribosomal peptide synthetase activity	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	586	590	PF00656	0.428
CLV_NRD_NRD_1	316	318	PF00675	0.255
CLV_NRD_NRD_1	522	524	PF00675	0.304
CLV_NRD_NRD_1	528	530	PF00675	0.274
CLV_PCSK_KEX2_1	316	318	PF00082	0.255
CLV_PCSK_KEX2_1	522	524	PF00082	0.287
CLV_PCSK_SKI1_1	103	107	PF00082	0.367
CLV_PCSK_SKI1_1	200	204	PF00082	0.261
CLV_PCSK_SKI1_1	301	305	PF00082	0.351
CLV_PCSK_SKI1_1	509	513	PF00082	0.308
CLV_PCSK_SKI1_1	7	11	PF00082	0.653
CLV_PCSK_SKI1_1	73	77	PF00082	0.288
DEG_APCC_DBOX_1	300	308	PF00400	0.329
DEG_APCC_DBOX_1	315	323	PF00400	0.253
DOC_CKS1_1	118	123	PF01111	0.385
DOC_CKS1_1	220	225	PF01111	0.217
DOC_CYCLIN_RxL_1	298	308	PF00134	0.304
DOC_CYCLIN_yCln2_LP_2	280	286	PF00134	0.230
DOC_CYCLIN_yCln2_LP_2	352	355	PF00134	0.304
DOC_MAPK_DCC_7	276	286	PF00069	0.304
DOC_MAPK_gen_1	198	206	PF00069	0.261
DOC_MAPK_JIP1_4	282	288	PF00069	0.385
DOC_MAPK_MEF2A_6	34	42	PF00069	0.340
DOC_MAPK_RevD_3	302	317	PF00069	0.302
DOC_PP1_RVXF_1	71	78	PF00149	0.297
DOC_PP1_SILK_1	134	139	PF00149	0.261
DOC_PP2B_LxvP_1	352	355	PF13499	0.304
DOC_USP7_MATH_1	15	19	PF00917	0.677
DOC_USP7_MATH_1	221	225	PF00917	0.269
DOC_USP7_MATH_1	265	269	PF00917	0.401
DOC_USP7_MATH_1	598	602	PF00917	0.240
DOC_WW_Pin1_4	117	122	PF00397	0.336
DOC_WW_Pin1_4	183	188	PF00397	0.274
DOC_WW_Pin1_4	219	224	PF00397	0.383
DOC_WW_Pin1_4	226	231	PF00397	0.380
LIG_14-3-3_CanoR_1	316	320	PF00244	0.272
LIG_14-3-3_CanoR_1	340	348	PF00244	0.375
LIG_14-3-3_CanoR_1	371	375	PF00244	0.292
LIG_Actin_RPEL_3	294	313	PF02755	0.173
LIG_Actin_WH2_2	300	318	PF00022	0.311
LIG_APCC_ABBAyCdc20_2	543	549	PF00400	0.151
LIG_BIR_III_4	566	570	PF00653	0.315
LIG_BRCT_BRCA1_1	178	182	PF00533	0.240
LIG_BRCT_BRCA1_1	84	88	PF00533	0.261
LIG_FHA_1	104	110	PF00498	0.255
LIG_FHA_1	117	123	PF00498	0.240
LIG_FHA_1	220	226	PF00498	0.374
LIG_FHA_1	244	250	PF00498	0.173
LIG_FHA_1	275	281	PF00498	0.298
LIG_FHA_1	307	313	PF00498	0.402
LIG_FHA_1	323	329	PF00498	0.166
LIG_FHA_1	342	348	PF00498	0.239
LIG_FHA_1	45	51	PF00498	0.361
LIG_FHA_2	340	346	PF00498	0.255
LIG_FHA_2	510	516	PF00498	0.392
LIG_LIR_Gen_1	201	211	PF02991	0.261
LIG_LIR_Gen_1	401	412	PF02991	0.255
LIG_LIR_Gen_1	450	458	PF02991	0.258
LIG_LIR_Gen_1	515	526	PF02991	0.331
LIG_LIR_Gen_1	85	95	PF02991	0.409
LIG_LIR_Nem_3	179	185	PF02991	0.290
LIG_LIR_Nem_3	201	206	PF02991	0.261
LIG_LIR_Nem_3	298	303	PF02991	0.274
LIG_LIR_Nem_3	450	456	PF02991	0.258
LIG_LIR_Nem_3	515	521	PF02991	0.385
LIG_LIR_Nem_3	85	91	PF02991	0.409
LIG_NRBOX	302	308	PF00104	0.304
LIG_Pex14_2	199	203	PF04695	0.261
LIG_Rb_pABgroove_1	65	73	PF01858	0.367
LIG_REV1ctd_RIR_1	538	547	PF16727	0.261
LIG_SH2_CRK	300	304	PF00017	0.333
LIG_SH2_CRK	453	457	PF00017	0.261
LIG_SH2_CRK	518	522	PF00017	0.255
LIG_SH2_STAP1	266	270	PF00017	0.261
LIG_SH2_STAP1	453	457	PF00017	0.261
LIG_SH2_STAP1	71	75	PF00017	0.367
LIG_SH2_STAT3	71	74	PF00017	0.385
LIG_SH2_STAT5	195	198	PF00017	0.305
LIG_SH2_STAT5	237	240	PF00017	0.282
LIG_SH2_STAT5	363	366	PF00017	0.263
LIG_SH2_STAT5	369	372	PF00017	0.217
LIG_SH2_STAT5	374	377	PF00017	0.207
LIG_SH2_STAT5	486	489	PF00017	0.297
LIG_SH2_STAT5	525	528	PF00017	0.240
LIG_SH2_STAT5	570	573	PF00017	0.376
LIG_SH3_3	125	131	PF00018	0.347
LIG_SH3_3	394	400	PF00018	0.248
LIG_SH3_3	419	425	PF00018	0.255
LIG_SH3_3	477	483	PF00018	0.261
LIG_SH3_3	484	490	PF00018	0.221
LIG_SUMO_SIM_par_1	108	114	PF11976	0.304
LIG_SUMO_SIM_par_1	302	309	PF11976	0.413
LIG_TRAF2_1	26	29	PF00917	0.439
LIG_TRFH_1	486	490	PF08558	0.240
LIG_UBA3_1	109	115	PF00899	0.311
LIG_WRC_WIRS_1	266	271	PF05994	0.261
LIG_WRC_WIRS_1	406	411	PF05994	0.255
MOD_CDK_SPK_2	226	231	PF00069	0.261
MOD_CDK_SPxxK_3	226	233	PF00069	0.239
MOD_CK1_1	101	107	PF00069	0.439
MOD_CK1_1	151	157	PF00069	0.446
MOD_CK1_1	219	225	PF00069	0.448
MOD_CK1_1	341	347	PF00069	0.438
MOD_CK1_1	417	423	PF00069	0.379
MOD_CK1_1	447	453	PF00069	0.407
MOD_CK1_1	94	100	PF00069	0.402
MOD_CK2_1	151	157	PF00069	0.357
MOD_CK2_1	24	30	PF00069	0.432
MOD_CK2_1	339	345	PF00069	0.237
MOD_CK2_1	467	473	PF00069	0.173
MOD_CK2_1	94	100	PF00069	0.385
MOD_GlcNHglycan	148	151	PF01048	0.337
MOD_GlcNHglycan	157	161	PF01048	0.316
MOD_GlcNHglycan	234	237	PF01048	0.306
MOD_GlcNHglycan	249	252	PF01048	0.216
MOD_GSK3_1	1	8	PF00069	0.680
MOD_GSK3_1	136	143	PF00069	0.383
MOD_GSK3_1	148	155	PF00069	0.270
MOD_GSK3_1	174	181	PF00069	0.287
MOD_GSK3_1	212	219	PF00069	0.359
MOD_GSK3_1	232	239	PF00069	0.154
MOD_GSK3_1	243	250	PF00069	0.324
MOD_GSK3_1	311	318	PF00069	0.255
MOD_GSK3_1	380	387	PF00069	0.358
MOD_GSK3_1	44	51	PF00069	0.379
MOD_GSK3_1	575	582	PF00069	0.425
MOD_GSK3_1	91	98	PF00069	0.361
MOD_N-GLC_1	243	248	PF02516	0.173
MOD_N-GLC_1	417	422	PF02516	0.385
MOD_N-GLC_1	81	86	PF02516	0.279
MOD_N-GLC_2	551	553	PF02516	0.247
MOD_NEK2_1	306	311	PF00069	0.305
MOD_NEK2_1	315	320	PF00069	0.261
MOD_NEK2_1	322	327	PF00069	0.133
MOD_NEK2_1	370	375	PF00069	0.259
MOD_NEK2_1	579	584	PF00069	0.367
MOD_NEK2_1	91	96	PF00069	0.282
MOD_NEK2_2	311	316	PF00069	0.275
MOD_NEK2_2	53	58	PF00069	0.385
MOD_PK_1	398	404	PF00069	0.255
MOD_PKA_2	232	238	PF00069	0.313
MOD_PKA_2	315	321	PF00069	0.304
MOD_PKA_2	339	345	PF00069	0.345
MOD_PKA_2	370	376	PF00069	0.302
MOD_PKA_2	82	88	PF00069	0.353
MOD_PKA_2	91	97	PF00069	0.257
MOD_Plk_1	101	107	PF00069	0.173
MOD_Plk_1	243	249	PF00069	0.302
MOD_Plk_2-3	339	345	PF00069	0.173
MOD_Plk_2-3	510	516	PF00069	0.304
MOD_Plk_4	221	227	PF00069	0.395
MOD_Plk_4	265	271	PF00069	0.261
MOD_Plk_4	370	376	PF00069	0.240
MOD_Plk_4	575	581	PF00069	0.354
MOD_ProDKin_1	117	123	PF00069	0.336
MOD_ProDKin_1	183	189	PF00069	0.274
MOD_ProDKin_1	219	225	PF00069	0.383
MOD_ProDKin_1	226	232	PF00069	0.380
MOD_SUMO_rev_2	464	469	PF00179	0.239
TRG_DiLeu_BaLyEn_6	105	110	PF01217	0.333
TRG_DiLeu_BaLyEn_6	298	303	PF01217	0.173
TRG_ENDOCYTIC_2	129	132	PF00928	0.297
TRG_ENDOCYTIC_2	266	269	PF00928	0.261
TRG_ENDOCYTIC_2	300	303	PF00928	0.333
TRG_ENDOCYTIC_2	404	407	PF00928	0.255
TRG_ENDOCYTIC_2	453	456	PF00928	0.261
TRG_ENDOCYTIC_2	518	521	PF00928	0.286
TRG_ENDOCYTIC_2	525	528	PF00928	0.285
TRG_ER_diArg_1	230	233	PF00400	0.400
TRG_ER_diArg_1	315	317	PF00400	0.276
TRG_ER_diArg_1	460	463	PF00400	0.282
TRG_ER_diArg_1	521	523	PF00400	0.287
TRG_Pf-PMV_PEXEL_1	301	305	PF00026	0.351
TRG_Pf-PMV_PEXEL_1	317	321	PF00026	0.163
TRG_Pf-PMV_PEXEL_1	506	510	PF00026	0.385
TRG_Pf-PMV_PEXEL_1	68	72	PF00026	0.195

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IHC1	Leptomonas seymouri	62%	95%
A0A0S4J7U4	Bodo saltans	38%	100%
A0A1X0NNJ6	Trypanosomatidae	43%	100%
A0A3Q8IC15	Leishmania donovani	91%	100%
A0A3R7LQL5	Trypanosoma rangeli	45%	100%
A4H7P4	Leishmania braziliensis	75%	96%
A4HW34	Leishmania infantum	91%	100%
C9ZSZ1	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	41%	100%
E9APT6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	87%	100%
O22494	Solanum lycopersicum	29%	100%
O23732	Brassica juncea	29%	100%
P35668	Xenopus laevis	28%	100%
P35669	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	28%	100%
P46413	Rattus norvegicus	28%	100%
P46416	Arabidopsis thaliana	30%	100%
P48637	Homo sapiens	29%	100%
P51855	Mus musculus	28%	100%
Q08220	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	27%	100%
Q54E83	Dictyostelium discoideum	26%	100%
Q5EAC2	Bos taurus	28%	100%
Q8HXX5	Macaca fascicularis	29%	100%
V5BNE3	Trypanosoma cruzi	46%	100%