PRK domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

hypothetical protein, conserved

Species:

Leishmania major

UniProt:

Q4QFN8_LEIMA

TriTrypDb:

LmjF.14.0970 , LMJLV39_140016400 , LMJSD75_140015800

Length:

287

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 9

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

Q4QFN8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QFN8

Function

Biological processes
Term	Name	Level	Count
GO:0006793	phosphorus metabolic process	3	1
GO:0006796	phosphate-containing compound metabolic process	4	1
GO:0008152	metabolic process	1	1
GO:0009987	cellular process	1	1
GO:0016310	phosphorylation	5	1
GO:0044237	cellular metabolic process	2	1

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	3
GO:0003824	catalytic activity	1	3
GO:0005488	binding	1	3
GO:0005524	ATP binding	5	3
GO:0016301	kinase activity	4	3
GO:0016740	transferase activity	2	3
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	3
GO:0017076	purine nucleotide binding	4	3
GO:0030554	adenyl nucleotide binding	5	3
GO:0032553	ribonucleotide binding	3	3
GO:0032555	purine ribonucleotide binding	4	3
GO:0032559	adenyl ribonucleotide binding	5	3
GO:0035639	purine ribonucleoside triphosphate binding	4	3
GO:0036094	small molecule binding	2	3
GO:0043167	ion binding	2	3
GO:0043168	anion binding	3	3
GO:0097159	organic cyclic compound binding	2	3
GO:0097367	carbohydrate derivative binding	2	3
GO:1901265	nucleoside phosphate binding	3	3
GO:1901363	heterocyclic compound binding	2	3

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	88	92	PF00656	0.707
CLV_NRD_NRD_1	124	126	PF00675	0.425
CLV_NRD_NRD_1	140	142	PF00675	0.426
CLV_NRD_NRD_1	247	249	PF00675	0.534
CLV_NRD_NRD_1	84	86	PF00675	0.621
CLV_PCSK_KEX2_1	139	141	PF00082	0.417
CLV_PCSK_SKI1_1	126	130	PF00082	0.525
CLV_PCSK_SKI1_1	222	226	PF00082	0.441
CLV_PCSK_SKI1_1	239	243	PF00082	0.528
CLV_PCSK_SKI1_1	71	75	PF00082	0.449
DEG_APCC_DBOX_1	124	132	PF00400	0.411
DEG_APCC_DBOX_1	152	160	PF00400	0.468
DEG_COP1_1	169	176	PF00400	0.426
DOC_CKS1_1	159	164	PF01111	0.567
DOC_MAPK_DCC_7	40	49	PF00069	0.567
DOC_MAPK_gen_1	151	159	PF00069	0.452
DOC_MAPK_MEF2A_6	40	49	PF00069	0.540
DOC_PP2B_LxvP_1	129	132	PF13499	0.576
DOC_USP7_MATH_1	255	259	PF00917	0.427
DOC_USP7_MATH_1	282	286	PF00917	0.645
DOC_WW_Pin1_4	158	163	PF00397	0.557
DOC_WW_Pin1_4	206	211	PF00397	0.400
LIG_14-3-3_CanoR_1	20	25	PF00244	0.463
LIG_Actin_WH2_2	17	35	PF00022	0.505
LIG_BIR_II_1	1	5	PF00653	0.628
LIG_CaM_IQ_9	234	250	PF13499	0.340
LIG_deltaCOP1_diTrp_1	144	147	PF00928	0.385
LIG_deltaCOP1_diTrp_1	211	219	PF00928	0.495
LIG_FHA_1	266	272	PF00498	0.461
LIG_FHA_2	159	165	PF00498	0.573
LIG_LIR_Apic_2	112	117	PF02991	0.462
LIG_LIR_Gen_1	209	219	PF02991	0.366
LIG_LIR_Nem_3	119	124	PF02991	0.342
LIG_LIR_Nem_3	200	206	PF02991	0.335
LIG_LIR_Nem_3	209	215	PF02991	0.363
LIG_PDZ_Class_1	282	287	PF00595	0.658
LIG_Pex14_2	172	176	PF04695	0.478
LIG_Rb_pABgroove_1	213	221	PF01858	0.490
LIG_SH2_NCK_1	168	172	PF00017	0.463
LIG_SH2_PTP2	114	117	PF00017	0.302
LIG_SH2_SRC	168	171	PF00017	0.471
LIG_SH2_STAP1	168	172	PF00017	0.471
LIG_SH2_STAT3	25	28	PF00017	0.517
LIG_SH2_STAT5	114	117	PF00017	0.425
LIG_SH2_STAT5	203	206	PF00017	0.347
LIG_SH3_2	80	85	PF14604	0.590
LIG_SH3_3	168	174	PF00018	0.389
LIG_SH3_3	177	183	PF00018	0.429
LIG_SH3_3	77	83	PF00018	0.587
LIG_SUMO_SIM_par_1	154	161	PF11976	0.476
LIG_SUMO_SIM_par_1	195	200	PF11976	0.353
LIG_SUMO_SIM_par_1	20	26	PF11976	0.514
LIG_TRAF2_1	10	13	PF00917	0.505
LIG_WRC_WIRS_1	173	178	PF05994	0.408
MOD_CDK_SPxxK_3	158	165	PF00069	0.573
MOD_CK1_1	158	164	PF00069	0.529
MOD_CK1_1	57	63	PF00069	0.365
MOD_CK1_1	96	102	PF00069	0.767
MOD_CK2_1	158	164	PF00069	0.568
MOD_CK2_1	20	26	PF00069	0.391
MOD_CK2_1	244	250	PF00069	0.483
MOD_CK2_1	7	13	PF00069	0.450
MOD_GlcNHglycan	1	4	PF01048	0.642
MOD_GlcNHglycan	106	109	PF01048	0.673
MOD_GlcNHglycan	192	195	PF01048	0.446
MOD_GlcNHglycan	255	258	PF01048	0.555
MOD_GlcNHglycan	56	59	PF01048	0.361
MOD_GSK3_1	143	150	PF00069	0.545
MOD_GSK3_1	253	260	PF00069	0.538
MOD_GSK3_1	276	283	PF00069	0.529
MOD_GSK3_1	54	61	PF00069	0.367
MOD_LATS_1	5	11	PF00433	0.539
MOD_N-GLC_1	280	285	PF02516	0.645
MOD_N-GLC_1	96	101	PF02516	0.679
MOD_NEK2_1	265	270	PF00069	0.383
MOD_NEK2_1	32	37	PF00069	0.506
MOD_PK_1	20	26	PF00069	0.452
MOD_PKA_2	253	259	PF00069	0.509
MOD_PKA_2	32	38	PF00069	0.621
MOD_Plk_1	143	149	PF00069	0.398
MOD_Plk_1	266	272	PF00069	0.506
MOD_Plk_4	20	26	PF00069	0.533
MOD_Plk_4	266	272	PF00069	0.456
MOD_ProDKin_1	158	164	PF00069	0.568
MOD_ProDKin_1	206	212	PF00069	0.393
MOD_SUMO_rev_2	217	224	PF00179	0.450
MOD_SUMO_rev_2	279	287	PF00179	0.615
TRG_ENDOCYTIC_2	168	171	PF00928	0.442
TRG_ER_diArg_1	138	141	PF00400	0.432
TRG_NES_CRM1_1	12	26	PF08389	0.476
TRG_NES_CRM1_1	79	93	PF08389	0.595
TRG_Pf-PMV_PEXEL_1	165	169	PF00026	0.499
TRG_PTS1	284	287	PF00515	0.617

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PB45	Leptomonas seymouri	65%	98%
A0A0S4JJ70	Bodo saltans	36%	100%
A0A1X0NT00	Trypanosomatidae	48%	100%
A0A3Q8IA01	Leishmania donovani	95%	100%
A0A3R7K1F1	Trypanosoma rangeli	47%	100%
A4H7Q1	Leishmania braziliensis	84%	100%
A4HW39	Leishmania infantum	96%	100%
A7A245	Saccharomyces cerevisiae (strain YJM789)	23%	81%
A7TQF3	Vanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294 / BCRC 21397 / CBS 2163 / NBRC 10782 / NRRL Y-8283 / UCD 57-17)	24%	80%
B3LUL5	Saccharomyces cerevisiae (strain RM11-1a)	23%	81%
B5VI33	Saccharomyces cerevisiae (strain AWRI1631)	23%	81%
C5DNG5	Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)	29%	83%
C7GYB3	Saccharomyces cerevisiae (strain JAY291)	23%	81%
C8Z7U0	Saccharomyces cerevisiae (strain Lalvin EC1118 / Prise de mousse)	23%	81%
E9APU2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	93%	100%
P43591	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	25%	81%
Q9UTC5	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	29%	100%
V5AZD0	Trypanosoma cruzi	53%	100%