Poly(A) polymerase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Poly(A) polymerase

Gene product:

poly(A) polymerase, putative

Species:

Leishmania major

UniProt:

Q4QFL6_LEIMA

TriTrypDb:

LmjF.14.1180 , LMJLV39_140019600 , LMJSD75_140019100

Length:

700

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 13
NetGPI	no	yes: 0, no: 13

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	14
GO:0005737	cytoplasm	2	14
GO:0043226	organelle	2	14
GO:0043227	membrane-bounded organelle	3	14
GO:0043229	intracellular organelle	3	14
GO:0043231	intracellular membrane-bounded organelle	4	14
GO:0110165	cellular anatomical entity	1	14
GO:0005654	nucleoplasm	2	1
GO:0005730	nucleolus	5	1
GO:0043228	non-membrane-bounded organelle	3	1
GO:0043232	intracellular non-membrane-bounded organelle	4	1

Expansion

Sequence features

Q4QFL6

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QFL6

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	14
GO:0006378	mRNA polyadenylation	7	3
GO:0006396	RNA processing	6	14
GO:0006397	mRNA processing	7	14
GO:0006725	cellular aromatic compound metabolic process	3	14
GO:0006807	nitrogen compound metabolic process	2	14
GO:0008152	metabolic process	1	14
GO:0009987	cellular process	1	14
GO:0016070	RNA metabolic process	5	14
GO:0016071	mRNA metabolic process	6	14
GO:0031123	RNA 3'-end processing	7	14
GO:0031124	mRNA 3'-end processing	8	3
GO:0034641	cellular nitrogen compound metabolic process	3	14
GO:0043170	macromolecule metabolic process	3	14
GO:0043631	RNA polyadenylation	6	14
GO:0044237	cellular metabolic process	2	14
GO:0044238	primary metabolic process	2	14
GO:0046483	heterocycle metabolic process	3	14
GO:0071076	RNA 3' uridylation	8	14
GO:0071704	organic substance metabolic process	2	14
GO:0090304	nucleic acid metabolic process	4	14
GO:1901360	organic cyclic compound metabolic process	3	14

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	14
GO:0003676	nucleic acid binding	3	14
GO:0003723	RNA binding	4	14
GO:0003824	catalytic activity	1	14
GO:0004652	obsolete polynucleotide adenylyltransferase activity	6	14
GO:0005488	binding	1	14
GO:0005524	ATP binding	5	14
GO:0016740	transferase activity	2	14
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	14
GO:0016779	nucleotidyltransferase activity	4	14
GO:0017076	purine nucleotide binding	4	14
GO:0030554	adenyl nucleotide binding	5	14
GO:0032553	ribonucleotide binding	3	14
GO:0032555	purine ribonucleotide binding	4	14
GO:0032559	adenyl ribonucleotide binding	5	14
GO:0035639	purine ribonucleoside triphosphate binding	4	14
GO:0036094	small molecule binding	2	14
GO:0043167	ion binding	2	14
GO:0043168	anion binding	3	14
GO:0043169	cation binding	3	14
GO:0046872	metal ion binding	4	14
GO:0050265	RNA uridylyltransferase activity	4	14
GO:0070566	adenylyltransferase activity	5	14
GO:0070569	uridylyltransferase activity	5	14
GO:0097159	organic cyclic compound binding	2	14
GO:0097367	carbohydrate derivative binding	2	14
GO:0140098	catalytic activity, acting on RNA	3	14
GO:0140640	catalytic activity, acting on a nucleic acid	2	14
GO:1901265	nucleoside phosphate binding	3	14
GO:1901363	heterocyclic compound binding	2	14

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	302	306	PF00656	0.267
CLV_C14_Caspase3-7	388	392	PF00656	0.250
CLV_C14_Caspase3-7	617	621	PF00656	0.783
CLV_C14_Caspase3-7	641	645	PF00656	0.777
CLV_NRD_NRD_1	434	436	PF00675	0.317
CLV_NRD_NRD_1	50	52	PF00675	0.286
CLV_NRD_NRD_1	647	649	PF00675	0.630
CLV_PCSK_KEX2_1	434	436	PF00082	0.290
CLV_PCSK_KEX2_1	49	51	PF00082	0.287
CLV_PCSK_KEX2_1	598	600	PF00082	0.585
CLV_PCSK_KEX2_1	649	651	PF00082	0.712
CLV_PCSK_PC1ET2_1	598	600	PF00082	0.585
CLV_PCSK_PC1ET2_1	649	651	PF00082	0.712
CLV_PCSK_SKI1_1	204	208	PF00082	0.329
CLV_PCSK_SKI1_1	212	216	PF00082	0.317
CLV_PCSK_SKI1_1	51	55	PF00082	0.278
CLV_PCSK_SKI1_1	9	13	PF00082	0.425
DEG_APCC_DBOX_1	207	215	PF00400	0.254
DEG_APCC_DBOX_1	50	58	PF00400	0.258
DEG_SPOP_SBC_1	604	608	PF00917	0.747
DEG_SPOP_SBC_1	667	671	PF00917	0.525
DOC_CKS1_1	312	317	PF01111	0.254
DOC_MAPK_DCC_7	140	149	PF00069	0.267
DOC_MAPK_FxFP_2	228	231	PF00069	0.267
DOC_MAPK_gen_1	300	309	PF00069	0.267
DOC_MAPK_HePTP_8	53	65	PF00069	0.401
DOC_MAPK_MEF2A_6	140	149	PF00069	0.273
DOC_MAPK_MEF2A_6	378	387	PF00069	0.322
DOC_MAPK_MEF2A_6	56	65	PF00069	0.401
DOC_PP1_RVXF_1	405	411	PF00149	0.274
DOC_PP2B_LxvP_1	108	111	PF13499	0.425
DOC_PP2B_PxIxI_1	544	550	PF00149	0.267
DOC_PP4_FxxP_1	228	231	PF00568	0.267
DOC_PP4_FxxP_1	436	439	PF00568	0.374
DOC_PP4_FxxP_1	73	76	PF00568	0.267
DOC_USP7_MATH_1	299	303	PF00917	0.306
DOC_USP7_MATH_1	457	461	PF00917	0.417
DOC_USP7_MATH_1	619	623	PF00917	0.820
DOC_USP7_MATH_1	639	643	PF00917	0.583
DOC_USP7_MATH_1	667	671	PF00917	0.740
DOC_USP7_MATH_1	672	676	PF00917	0.718
DOC_USP7_MATH_1	89	93	PF00917	0.443
DOC_USP7_MATH_2	299	305	PF00917	0.267
DOC_USP7_UBL2_3	215	219	PF12436	0.254
DOC_WW_Pin1_4	311	316	PF00397	0.254
LIG_14-3-3_CanoR_1	126	131	PF00244	0.270
LIG_14-3-3_CanoR_1	208	212	PF00244	0.358
LIG_14-3-3_CanoR_1	258	264	PF00244	0.334
LIG_14-3-3_CanoR_1	288	297	PF00244	0.401
LIG_14-3-3_CanoR_1	417	422	PF00244	0.301
LIG_14-3-3_CanoR_1	49	54	PF00244	0.296
LIG_14-3-3_CanoR_1	525	531	PF00244	0.381
LIG_14-3-3_CanoR_1	66	70	PF00244	0.254
LIG_BRCT_BRCA1_1	224	228	PF00533	0.267
LIG_EH1_1	194	202	PF00400	0.285
LIG_FHA_1	171	177	PF00498	0.282
LIG_FHA_1	238	244	PF00498	0.401
LIG_FHA_1	312	318	PF00498	0.263
LIG_FHA_2	272	278	PF00498	0.361
LIG_FHA_2	3	9	PF00498	0.519
LIG_FHA_2	37	43	PF00498	0.362
LIG_FHA_2	418	424	PF00498	0.401
LIG_FHA_2	546	552	PF00498	0.259
LIG_LIR_Apic_2	225	231	PF02991	0.267
LIG_LIR_Apic_2	265	269	PF02991	0.286
LIG_LIR_Apic_2	71	76	PF02991	0.267
LIG_LIR_Gen_1	117	127	PF02991	0.451
LIG_LIR_Gen_1	526	534	PF02991	0.345
LIG_LIR_Nem_3	117	123	PF02991	0.451
LIG_LIR_Nem_3	157	163	PF02991	0.303
LIG_LIR_Nem_3	257	263	PF02991	0.406
LIG_LIR_Nem_3	393	398	PF02991	0.289
LIG_LIR_Nem_3	422	427	PF02991	0.310
LIG_LIR_Nem_3	526	530	PF02991	0.345
LIG_MYND_1	311	315	PF01753	0.306
LIG_PDZ_Class_2	695	700	PF00595	0.503
LIG_Pex14_2	213	217	PF04695	0.254
LIG_Pex14_2	375	379	PF04695	0.295
LIG_PTB_Apo_2	318	325	PF02174	0.254
LIG_PTB_Phospho_1	318	324	PF10480	0.285
LIG_REV1ctd_RIR_1	257	265	PF16727	0.250
LIG_REV1ctd_RIR_1	373	382	PF16727	0.401
LIG_RPA_C_Fungi	580	592	PF08784	0.522
LIG_SH2_CRK	424	428	PF00017	0.401
LIG_SH2_GRB2like	224	227	PF00017	0.267
LIG_SH2_SRC	398	401	PF00017	0.301
LIG_SH2_STAP1	224	228	PF00017	0.274
LIG_SH2_STAT3	247	250	PF00017	0.401
LIG_SH2_STAT5	282	285	PF00017	0.313
LIG_SH2_STAT5	382	385	PF00017	0.254
LIG_SH2_STAT5	503	506	PF00017	0.257
LIG_SH2_STAT5	565	568	PF00017	0.548
LIG_SH3_3	309	315	PF00018	0.315
LIG_SH3_3	366	372	PF00018	0.454
LIG_SH3_3	490	496	PF00018	0.363
LIG_TRAF2_1	43	46	PF00917	0.337
LIG_TRAF2_1	571	574	PF00917	0.517
LIG_UBA3_1	197	204	PF00899	0.267
LIG_WRC_WIRS_1	524	529	PF05994	0.345
LIG_WRC_WIRS_1	555	560	PF05994	0.437
MOD_CK1_1	112	118	PF00069	0.404
MOD_CK1_1	159	165	PF00069	0.254
MOD_CK1_1	184	190	PF00069	0.306
MOD_CK1_1	222	228	PF00069	0.297
MOD_CK1_1	450	456	PF00069	0.303
MOD_CK1_1	465	471	PF00069	0.213
MOD_CK1_1	491	497	PF00069	0.420
MOD_CK1_1	603	609	PF00069	0.641
MOD_CK1_1	610	616	PF00069	0.722
MOD_CK1_1	624	630	PF00069	0.628
MOD_CK1_1	642	648	PF00069	0.630
MOD_CK2_1	271	277	PF00069	0.285
MOD_CK2_1	29	35	PF00069	0.356
MOD_CK2_1	349	355	PF00069	0.407
MOD_CK2_1	36	42	PF00069	0.324
MOD_CK2_1	387	393	PF00069	0.267
MOD_CK2_1	49	55	PF00069	0.153
MOD_CK2_1	574	580	PF00069	0.695
MOD_CK2_1	619	625	PF00069	0.734
MOD_GlcNHglycan	17	20	PF01048	0.395
MOD_GlcNHglycan	231	234	PF01048	0.285
MOD_GlcNHglycan	351	354	PF01048	0.466
MOD_GlcNHglycan	470	473	PF01048	0.328
MOD_GlcNHglycan	489	493	PF01048	0.357
MOD_GlcNHglycan	514	517	PF01048	0.344
MOD_GlcNHglycan	602	605	PF01048	0.775
MOD_GlcNHglycan	616	619	PF01048	0.644
MOD_GlcNHglycan	620	624	PF01048	0.587
MOD_GlcNHglycan	674	677	PF01048	0.672
MOD_GlcNHglycan	99	102	PF01048	0.277
MOD_GSK3_1	413	420	PF00069	0.325
MOD_GSK3_1	443	450	PF00069	0.354
MOD_GSK3_1	487	494	PF00069	0.417
MOD_GSK3_1	508	515	PF00069	0.365
MOD_GSK3_1	599	606	PF00069	0.628
MOD_GSK3_1	607	614	PF00069	0.639
MOD_GSK3_1	619	626	PF00069	0.706
MOD_GSK3_1	638	645	PF00069	0.618
MOD_GSK3_1	668	675	PF00069	0.651
MOD_N-GLC_1	324	329	PF02516	0.254
MOD_N-GLC_1	412	417	PF02516	0.401
MOD_N-GLC_1	585	590	PF02516	0.772
MOD_N-GLC_2	321	323	PF02516	0.254
MOD_NEK2_1	1	6	PF00069	0.626
MOD_NEK2_1	119	124	PF00069	0.277
MOD_NEK2_1	207	212	PF00069	0.407
MOD_NEK2_1	259	264	PF00069	0.430
MOD_NEK2_1	283	288	PF00069	0.254
MOD_NEK2_1	349	354	PF00069	0.534
MOD_NEK2_2	319	324	PF00069	0.295
MOD_NEK2_2	477	482	PF00069	0.195
MOD_NEK2_2	523	528	PF00069	0.301
MOD_PK_1	219	225	PF00069	0.295
MOD_PK_1	49	55	PF00069	0.285
MOD_PKA_1	49	55	PF00069	0.315
MOD_PKA_1	648	654	PF00069	0.573
MOD_PKA_2	112	118	PF00069	0.259
MOD_PKA_2	139	145	PF00069	0.311
MOD_PKA_2	207	213	PF00069	0.261
MOD_PKA_2	299	305	PF00069	0.272
MOD_PKA_2	446	452	PF00069	0.315
MOD_PKA_2	462	468	PF00069	0.300
MOD_PKA_2	49	55	PF00069	0.295
MOD_PKA_2	575	581	PF00069	0.533
MOD_PKA_2	65	71	PF00069	0.254
MOD_PKB_1	445	453	PF00069	0.401
MOD_Plk_1	156	162	PF00069	0.254
MOD_Plk_1	181	187	PF00069	0.254
MOD_Plk_1	29	35	PF00069	0.410
MOD_Plk_1	324	330	PF00069	0.267
MOD_Plk_2-3	170	176	PF00069	0.254
MOD_Plk_4	181	187	PF00069	0.295
MOD_Plk_4	262	268	PF00069	0.303
MOD_Plk_4	494	500	PF00069	0.427
MOD_Plk_4	526	532	PF00069	0.322
MOD_Plk_4	554	560	PF00069	0.372
MOD_Plk_4	65	71	PF00069	0.254
MOD_ProDKin_1	311	317	PF00069	0.254
MOD_SUMO_for_1	127	130	PF00179	0.401
MOD_SUMO_for_1	665	668	PF00179	0.749
MOD_SUMO_rev_2	298	304	PF00179	0.269
MOD_SUMO_rev_2	352	358	PF00179	0.369
TRG_ENDOCYTIC_2	382	385	PF00928	0.254
TRG_ENDOCYTIC_2	424	427	PF00928	0.401
TRG_ER_diArg_1	287	290	PF00400	0.295
TRG_ER_diArg_1	405	408	PF00400	0.401
TRG_ER_diArg_1	433	435	PF00400	0.284
TRG_ER_diArg_1	444	447	PF00400	0.282
TRG_ER_diArg_1	49	51	PF00400	0.294
TRG_NLS_MonoExtC_3	647	652	PF00514	0.691
TRG_Pf-PMV_PEXEL_1	332	336	PF00026	0.287
TRG_Pf-PMV_PEXEL_1	532	536	PF00026	0.267
TRG_Pf-PMV_PEXEL_1	628	632	PF00026	0.583

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I2K1	Leptomonas seymouri	73%	95%
A0A0S4JIG5	Bodo saltans	47%	100%
A0A1X0NP17	Trypanosomatidae	61%	100%
A0A3Q8ID77	Leishmania donovani	94%	99%
A0A3R7KE83	Trypanosoma rangeli	59%	100%
A4H7T8	Leishmania braziliensis	85%	99%
A4HW64	Leishmania infantum	94%	99%
A4I4T6	Leishmania infantum	27%	100%
C9ZSW0	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	59%	100%
E9AEC2	Leishmania major	26%	100%
E9APW5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	94%	99%
V5BKK6	Trypanosoma cruzi	59%	85%