PPM-type phosphatase domain-containing protein

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

protein phosphatase 2C, putative

Species:

Leishmania major

UniProt:

Q4QFG7_LEIMA

TriTrypDb:

LmjF.15.0170 , LMJLV39_150006800 , LMJSD75_150006900

Length:

404

Annotations

LeishMANIAdb annotations

This large family encompasses many diverse protein phosphatases. Some appear to have evolved transmembrane segments. Very tentatively they might regulate transmembrane receptor kinases.. The TM and non-TM groups diverged early in Eukaryota and appear to be distinct enough that they probably should not be part of the same cluster. This latter group has not expanded.

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 20

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 19
NetGPI	no	yes: 0, no: 19

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	3
GO:0110165	cellular anatomical entity	1	4
GO:0016020	membrane	2	1
GO:0031974	membrane-enclosed lumen	2	1
GO:0031981	nuclear lumen	5	1
GO:0032838	plasma membrane bounded cell projection cytoplasm	4	1
GO:0043233	organelle lumen	3	1
GO:0070013	intracellular organelle lumen	4	1
GO:0097014	ciliary plasm	5	1
GO:0099568	cytoplasmic region	3	1

Expansion

Sequence features

Q4QFG7

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QFG7

Function

Biological processes
Term	Name	Level	Count
GO:0006470	protein dephosphorylation	5	20
GO:0006793	phosphorus metabolic process	3	20
GO:0006796	phosphate-containing compound metabolic process	4	20
GO:0006807	nitrogen compound metabolic process	2	20
GO:0008152	metabolic process	1	20
GO:0009987	cellular process	1	20
GO:0016311	dephosphorylation	5	20
GO:0019538	protein metabolic process	3	20
GO:0036211	protein modification process	4	20
GO:0043170	macromolecule metabolic process	3	20
GO:0043412	macromolecule modification	4	20
GO:0044237	cellular metabolic process	2	20
GO:0044238	primary metabolic process	2	20
GO:0071704	organic substance metabolic process	2	20
GO:1901564	organonitrogen compound metabolic process	3	20

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	20
GO:0004721	phosphoprotein phosphatase activity	3	20
GO:0004722	protein serine/threonine phosphatase activity	4	20
GO:0016787	hydrolase activity	2	20
GO:0016788	hydrolase activity, acting on ester bonds	3	20
GO:0016791	phosphatase activity	5	20
GO:0042578	phosphoric ester hydrolase activity	4	20
GO:0140096	catalytic activity, acting on a protein	2	20
GO:0017018	myosin phosphatase activity	5	3
GO:0005488	binding	1	1
GO:0043167	ion binding	2	1
GO:0043169	cation binding	3	1
GO:0046872	metal ion binding	4	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	126	130	PF00656	0.527
CLV_C14_Caspase3-7	330	334	PF00656	0.425
CLV_C14_Caspase3-7	391	395	PF00656	0.380
CLV_NRD_NRD_1	6	8	PF00675	0.739
CLV_NRD_NRD_1	71	73	PF00675	0.555
CLV_PCSK_KEX2_1	306	308	PF00082	0.412
CLV_PCSK_KEX2_1	310	312	PF00082	0.405
CLV_PCSK_KEX2_1	328	330	PF00082	0.480
CLV_PCSK_KEX2_1	6	8	PF00082	0.736
CLV_PCSK_PC1ET2_1	306	308	PF00082	0.420
CLV_PCSK_PC1ET2_1	310	312	PF00082	0.405
CLV_PCSK_PC1ET2_1	328	330	PF00082	0.480
CLV_PCSK_PC1ET2_1	6	8	PF00082	0.736
CLV_PCSK_SKI1_1	117	121	PF00082	0.454
CLV_PCSK_SKI1_1	194	198	PF00082	0.338
CLV_PCSK_SKI1_1	210	214	PF00082	0.377
CLV_PCSK_SKI1_1	217	221	PF00082	0.310
CLV_PCSK_SKI1_1	251	255	PF00082	0.314
CLV_PCSK_SKI1_1	311	315	PF00082	0.339
CLV_PCSK_SKI1_1	359	363	PF00082	0.299
CLV_PCSK_SKI1_1	60	64	PF00082	0.534
DEG_Nend_UBRbox_1	1	4	PF02207	0.583
DOC_CKS1_1	195	200	PF01111	0.365
DOC_MAPK_gen_1	359	369	PF00069	0.243
DOC_PP2B_LxvP_1	317	320	PF13499	0.258
DOC_USP7_MATH_1	113	117	PF00917	0.578
DOC_USP7_MATH_1	331	335	PF00917	0.411
DOC_USP7_UBL2_3	2	6	PF12436	0.658
DOC_USP7_UBL2_3	306	310	PF12436	0.355
DOC_WW_Pin1_4	194	199	PF00397	0.369
DOC_WW_Pin1_4	235	240	PF00397	0.351
DOC_WW_Pin1_4	379	384	PF00397	0.469
LIG_14-3-3_CanoR_1	94	99	PF00244	0.622
LIG_BRCT_BRCA1_1	184	188	PF00533	0.288
LIG_CtBP_PxDLS_1	239	243	PF00389	0.199
LIG_FHA_1	232	238	PF00498	0.326
LIG_FHA_1	312	318	PF00498	0.296
LIG_FHA_1	395	401	PF00498	0.353
LIG_FHA_1	48	54	PF00498	0.572
LIG_FHA_1	82	88	PF00498	0.657
LIG_FHA_2	124	130	PF00498	0.402
LIG_FHA_2	307	313	PF00498	0.315
LIG_LIR_Apic_2	193	198	PF02991	0.258
LIG_LIR_Apic_2	234	239	PF02991	0.258
LIG_LIR_Gen_1	209	219	PF02991	0.309
LIG_LIR_Gen_1	333	343	PF02991	0.309
LIG_LIR_Gen_1	36	46	PF02991	0.482
LIG_LIR_LC3C_4	314	319	PF02991	0.366
LIG_LIR_Nem_3	166	172	PF02991	0.315
LIG_LIR_Nem_3	209	215	PF02991	0.280
LIG_LIR_Nem_3	333	338	PF02991	0.309
LIG_LIR_Nem_3	341	347	PF02991	0.310
LIG_LIR_Nem_3	36	42	PF02991	0.487
LIG_NRBOX	99	105	PF00104	0.565
LIG_PCNA_yPIPBox_3	94	108	PF02747	0.502
LIG_Pex14_2	208	212	PF04695	0.344
LIG_PTB_Apo_2	127	134	PF02174	0.417
LIG_PTB_Apo_2	151	158	PF02174	0.288
LIG_RPA_C_Fungi	89	101	PF08784	0.450
LIG_SH2_CRK	195	199	PF00017	0.450
LIG_SH2_NCK_1	195	199	PF00017	0.403
LIG_SH2_PTP2	236	239	PF00017	0.429
LIG_SH2_PTP2	335	338	PF00017	0.429
LIG_SH2_STAT5	168	171	PF00017	0.479
LIG_SH2_STAT5	236	239	PF00017	0.449
LIG_SH2_STAT5	335	338	PF00017	0.429
LIG_SH3_3	316	322	PF00018	0.391
LIG_SH3_3	377	383	PF00018	0.288
LIG_SUMO_SIM_anti_2	397	402	PF11976	0.356
LIG_SUMO_SIM_par_1	396	402	PF11976	0.324
LIG_TRAF2_1	238	241	PF00917	0.408
LIG_TRAF2_1	348	351	PF00917	0.318
LIG_UBA3_1	26	32	PF00899	0.448
LIG_UBA3_1	352	360	PF00899	0.429
LIG_UBA3_1	399	404	PF00899	0.387
MOD_CK1_1	161	167	PF00069	0.374
MOD_CK1_1	228	234	PF00069	0.337
MOD_CK1_1	44	50	PF00069	0.539
MOD_CK1_1	9	15	PF00069	0.518
MOD_CK2_1	187	193	PF00069	0.474
MOD_CK2_1	235	241	PF00069	0.421
MOD_CK2_1	306	312	PF00069	0.345
MOD_CK2_1	345	351	PF00069	0.298
MOD_CK2_1	378	384	PF00069	0.431
MOD_CK2_1	80	86	PF00069	0.587
MOD_GlcNHglycan	158	161	PF01048	0.365
MOD_GlcNHglycan	175	178	PF01048	0.276
MOD_GlcNHglycan	179	182	PF01048	0.273
MOD_GlcNHglycan	189	192	PF01048	0.258
MOD_GlcNHglycan	227	230	PF01048	0.335
MOD_GlcNHglycan	329	332	PF01048	0.439
MOD_GSK3_1	113	120	PF00069	0.522
MOD_GSK3_1	121	128	PF00069	0.513
MOD_GSK3_1	173	180	PF00069	0.493
MOD_GSK3_1	182	189	PF00069	0.417
MOD_GSK3_1	231	238	PF00069	0.299
MOD_GSK3_1	323	330	PF00069	0.429
MOD_GSK3_1	390	397	PF00069	0.408
MOD_N-GLC_1	14	19	PF02516	0.621
MOD_N-GLC_1	394	399	PF02516	0.350
MOD_N-GLC_2	182	184	PF02516	0.243
MOD_N-GLC_2	375	377	PF02516	0.465
MOD_NEK2_1	294	299	PF00069	0.308
MOD_NEK2_1	46	51	PF00069	0.618
MOD_PIKK_1	182	188	PF00454	0.418
MOD_PIKK_1	311	317	PF00454	0.304
MOD_PKA_1	306	312	PF00069	0.429
MOD_PKA_1	6	12	PF00069	0.602
MOD_PKA_2	306	312	PF00069	0.213
MOD_PKA_2	6	12	PF00069	0.531
MOD_PKA_2	81	87	PF00069	0.628
MOD_Plk_1	394	400	PF00069	0.351
MOD_Plk_4	231	237	PF00069	0.393
MOD_Plk_4	331	337	PF00069	0.318
MOD_Plk_4	394	400	PF00069	0.353
MOD_ProDKin_1	194	200	PF00069	0.369
MOD_ProDKin_1	235	241	PF00069	0.351
MOD_ProDKin_1	379	385	PF00069	0.469
MOD_SUMO_rev_2	262	269	PF00179	0.321
MOD_SUMO_rev_2	354	361	PF00179	0.258
TRG_DiLeu_BaEn_1	148	153	PF01217	0.344
TRG_DiLeu_BaEn_3	350	356	PF01217	0.421
TRG_ENDOCYTIC_2	168	171	PF00928	0.258
TRG_ENDOCYTIC_2	335	338	PF00928	0.429
TRG_NLS_MonoExtC_3	71	77	PF00514	0.600
TRG_NLS_MonoExtN_4	4	10	PF00514	0.724
TRG_Pf-PMV_PEXEL_1	41	45	PF00026	0.588

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P5C7	Leptomonas seymouri	71%	100%
A0A0N0P8F8	Leptomonas seymouri	38%	100%
A0A0N1I0H9	Leptomonas seymouri	38%	100%
A0A0N1I8W9	Leptomonas seymouri	30%	93%
A0A0N1IHU6	Leptomonas seymouri	30%	99%
A0A0N1PAL8	Leptomonas seymouri	32%	100%
A0A0N1PEL9	Leptomonas seymouri	28%	100%
A0A0N1PET0	Leptomonas seymouri	37%	69%
A0A0S4IY95	Bodo saltans	36%	100%
A0A0S4J0A6	Bodo saltans	31%	99%
A0A0S4JHB4	Bodo saltans	32%	100%
A0A0S4JPC3	Bodo saltans	42%	100%
A0A0S4JPM8	Bodo saltans	47%	100%
A0A0S4KIS4	Bodo saltans	34%	89%
A0A0S4KJV9	Bodo saltans	40%	93%
A0A1X0NRG3	Trypanosomatidae	35%	100%
A0A1X0NVC4	Trypanosomatidae	52%	98%
A0A1X0P568	Trypanosomatidae	33%	99%
A0A1X0P991	Trypanosomatidae	39%	100%
A0A1X0PAN4	Trypanosomatidae	29%	100%
A0A3Q8IEK6	Leishmania donovani	41%	100%
A0A3Q8IFG7	Leishmania donovani	32%	100%
A0A3Q8IGS1	Leishmania donovani	35%	72%
A0A3Q8IK65	Leishmania donovani	39%	100%
A0A3Q8IL63	Leishmania donovani	27%	100%
A0A3S7WTA2	Leishmania donovani	95%	100%
A0A3S7WZ14	Leishmania donovani	31%	100%
A0A3S7X808	Leishmania donovani	28%	90%
A0A422N8D7	Trypanosoma rangeli	28%	100%
A0A422NAJ1	Trypanosoma rangeli	37%	100%
A0A422NBV9	Trypanosoma rangeli	32%	99%
A0A422NL94	Trypanosoma rangeli	38%	92%
A0A422NML6	Trypanosoma rangeli	32%	100%
A0A422NS77	Trypanosoma rangeli	50%	98%
A0A422P293	Trypanosoma rangeli	39%	100%
A0BLX0	Paramecium tetraurelia	35%	100%
A0BQL0	Paramecium tetraurelia	35%	100%
A0CUB5	Paramecium tetraurelia	32%	100%
A0DSB3	Paramecium tetraurelia	33%	100%
A0DTY1	Paramecium tetraurelia	36%	100%
A3A8W2	Oryza sativa subsp. japonica	30%	100%
A3A8W6	Oryza sativa subsp. japonica	26%	70%
A4H7Y6	Leishmania braziliensis	79%	100%
A4HAW6	Leishmania braziliensis	28%	90%
A4HE10	Leishmania braziliensis	29%	100%
A4HG10	Leishmania braziliensis	41%	100%
A4HHY5	Leishmania braziliensis	33%	100%
A4HKF6	Leishmania braziliensis	35%	71%
A4HNR1	Leishmania braziliensis	38%	100%
A4HWB4	Leishmania infantum	95%	100%
A4I1B7	Leishmania infantum	31%	100%
A4I329	Leishmania infantum	41%	100%
A4I565	Leishmania infantum	32%	100%
A4I7Y4	Leishmania infantum	35%	72%
A4IA25	Leishmania infantum	27%	100%
A4IA26	Leishmania infantum	28%	90%
A4ICT4	Leishmania infantum	39%	100%
A5PJZ2	Bos taurus	32%	100%
C9ZJK2	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	36%	100%
C9ZMJ7	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	28%	100%
C9ZNW4	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	48%	95%
D0A2L9	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	38%	100%
D0A5L0	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	34%	99%
E9ACV0	Leishmania major	41%	100%
E9AQ14	Leishmania mexicana (strain MHOM/GT/2001/U1103)	91%	100%
E9ASH0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	37%	100%
E9AXF3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	31%	100%
E9AZD9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	41%	100%
E9B0G2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	32%	100%
E9B2U5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	35%	72%
E9B540	Leishmania mexicana (strain MHOM/GT/2001/U1103)	28%	100%
E9B541	Leishmania mexicana (strain MHOM/GT/2001/U1103)	28%	100%
O62829	Bos taurus	36%	100%
O62830	Bos taurus	36%	83%
O75688	Homo sapiens	36%	84%
O81716	Arabidopsis thaliana	33%	100%
P20650	Rattus norvegicus	36%	100%
P34221	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	38%	86%
P35182	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	31%	100%
P35813	Homo sapiens	36%	100%
P35814	Oryctolagus cuniculus	36%	100%
P35815	Rattus norvegicus	38%	100%
P36982	Leishmania chagasi	31%	100%
P36993	Mus musculus	37%	100%
P38089	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	30%	100%
P39966	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	36%	87%
P40371	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	30%	100%
P49443	Mus musculus	36%	100%
P49444	Paramecium tetraurelia	35%	100%
P49593	Homo sapiens	29%	89%
P49594	Caenorhabditis elegans	26%	90%
P49596	Caenorhabditis elegans	39%	100%
P49599	Arabidopsis thaliana	28%	100%
Q09172	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	40%	100%
Q09173	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	36%	98%
Q0IIF0	Bos taurus	31%	100%
Q0J2L7	Oryza sativa subsp. japonica	33%	100%
Q0JL75	Oryza sativa subsp. japonica	30%	100%
Q0JLP9	Oryza sativa subsp. japonica	33%	87%
Q10MX1	Oryza sativa subsp. japonica	29%	100%
Q12511	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	25%	71%
Q19775	Caenorhabditis elegans	36%	86%
Q2QWE3	Oryza sativa subsp. japonica	27%	96%
Q2R637	Oryza sativa subsp. japonica	28%	94%
Q4Q225	Leishmania major	37%	99%
Q4Q2U5	Leishmania major	28%	90%
Q4Q2U6	Leishmania major	27%	100%
Q4Q5B1	Leishmania major	36%	72%
Q4Q7S1	Leishmania major	31%	100%
Q4QA19	Leishmania major	31%	100%
Q53Q11	Oryza sativa subsp. japonica	30%	100%
Q5SGD2	Homo sapiens	32%	100%
Q5SMK6	Oryza sativa subsp. japonica	28%	100%
Q5SN75	Oryza sativa subsp. japonica	30%	100%
Q653S3	Oryza sativa subsp. japonica	33%	100%
Q65XG6	Oryza sativa subsp. japonica	33%	97%
Q67UP9	Oryza sativa subsp. japonica	34%	100%
Q67UX7	Oryza sativa subsp. japonica	31%	100%
Q69QZ0	Oryza sativa subsp. japonica	28%	100%
Q69VD9	Oryza sativa subsp. japonica	30%	100%
Q6AUQ4	Oryza sativa subsp. japonica	30%	100%
Q6ETK3	Oryza sativa subsp. japonica	33%	100%
Q6K1U4	Oryza sativa subsp. japonica	26%	78%
Q6K5I0	Oryza sativa subsp. japonica	25%	78%
Q6L5H6	Oryza sativa subsp. japonica	32%	100%
Q6NKS1	Arabidopsis thaliana	30%	100%
Q7XJ53	Arabidopsis thaliana	27%	100%
Q7XU84	Oryza sativa subsp. japonica	29%	100%
Q8BHN0	Mus musculus	32%	100%
Q8CGA0	Mus musculus	30%	89%
Q8H4S6	Oryza sativa subsp. japonica	32%	95%
Q8LAY8	Arabidopsis thaliana	31%	100%
Q8R0F6	Mus musculus	32%	100%
Q93YS2	Arabidopsis thaliana	24%	77%
Q9CAJ0	Arabidopsis thaliana	32%	79%
Q9FYN7	Oryza sativa subsp. japonica	28%	100%
Q9H0C8	Homo sapiens	32%	100%
Q9LMT1	Arabidopsis thaliana	24%	100%
Q9LNP9	Arabidopsis thaliana	30%	79%
Q9M1V8	Arabidopsis thaliana	25%	96%
Q9SLA1	Arabidopsis thaliana	32%	100%
Q9SZ53	Arabidopsis thaliana	33%	100%
Q9WVR7	Rattus norvegicus	31%	90%
Q9Z1Z6	Rattus norvegicus	32%	100%
V5AZJ4	Trypanosoma cruzi	38%	100%
V5BA03	Trypanosoma cruzi	41%	100%
V5BCX6	Trypanosoma cruzi	33%	100%
V5BH00	Trypanosoma cruzi	32%	100%
V5BSS7	Trypanosoma cruzi	32%	99%
V5DCR6	Trypanosoma cruzi	51%	98%