tRNA_SAD domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

hypothetical protein, conserved

Species:

Leishmania major

UniProt:

Q4QFB6_LEIMA

TriTrypDb:

LmjF.15.0690 , LMJLV39_150012700 , LMJSD75_150012800

Length:

491

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

Q4QFB6

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QFB6

Function

Biological processes
Term	Name	Level	Count
GO:0006450	regulation of translational fidelity	3	2
GO:0065007	biological regulation	1	2
GO:0065008	regulation of biological quality	2	2
GO:0006082	organic acid metabolic process	3	4
GO:0006139	nucleobase-containing compound metabolic process	3	4
GO:0006399	tRNA metabolic process	7	4
GO:0006520	amino acid metabolic process	3	4
GO:0006725	cellular aromatic compound metabolic process	3	4
GO:0006807	nitrogen compound metabolic process	2	4
GO:0008152	metabolic process	1	4
GO:0009987	cellular process	1	4
GO:0016070	RNA metabolic process	5	4
GO:0019752	carboxylic acid metabolic process	5	4
GO:0034641	cellular nitrogen compound metabolic process	3	4
GO:0034660	ncRNA metabolic process	6	4
GO:0043038	amino acid activation	4	4
GO:0043039	tRNA aminoacylation	5	4
GO:0043170	macromolecule metabolic process	3	4
GO:0043436	oxoacid metabolic process	4	4
GO:0044237	cellular metabolic process	2	4
GO:0044238	primary metabolic process	2	4
GO:0044281	small molecule metabolic process	2	4
GO:0046483	heterocycle metabolic process	3	4
GO:0071704	organic substance metabolic process	2	4
GO:0090304	nucleic acid metabolic process	4	4
GO:1901360	organic cyclic compound metabolic process	3	4
GO:1901564	organonitrogen compound metabolic process	3	4
GO:0006418	tRNA aminoacylation for protein translation	6	3
GO:0006419	alanyl-tRNA aminoacylation	7	3

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	11
GO:0002161	aminoacyl-tRNA editing activity	5	2
GO:0002196	Ser-tRNA(Ala) hydrolase activity	6	2
GO:0003824	catalytic activity	1	6
GO:0005488	binding	1	11
GO:0016787	hydrolase activity	2	2
GO:0016788	hydrolase activity, acting on ester bonds	3	2
GO:0036094	small molecule binding	2	11
GO:0052689	carboxylic ester hydrolase activity	4	2
GO:0097159	organic cyclic compound binding	2	11
GO:0140098	catalytic activity, acting on RNA	3	6
GO:0140101	catalytic activity, acting on a tRNA	4	6
GO:0140640	catalytic activity, acting on a nucleic acid	2	6
GO:1901265	nucleoside phosphate binding	3	11
GO:1901363	heterocyclic compound binding	2	11
GO:0004812	aminoacyl-tRNA ligase activity	4	4
GO:0005524	ATP binding	5	4
GO:0016874	ligase activity	2	4
GO:0016875	ligase activity, forming carbon-oxygen bonds	3	4
GO:0017076	purine nucleotide binding	4	4
GO:0030554	adenyl nucleotide binding	5	4
GO:0032553	ribonucleotide binding	3	4
GO:0032555	purine ribonucleotide binding	4	4
GO:0032559	adenyl ribonucleotide binding	5	4
GO:0035639	purine ribonucleoside triphosphate binding	4	4
GO:0043167	ion binding	2	4
GO:0043168	anion binding	3	4
GO:0097367	carbohydrate derivative binding	2	4
GO:0004813	alanine-tRNA ligase activity	5	3
GO:0003676	nucleic acid binding	3	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	270	274	PF00656	0.412
CLV_MEL_PAP_1	4	10	PF00089	0.412
CLV_NRD_NRD_1	103	105	PF00675	0.377
CLV_NRD_NRD_1	131	133	PF00675	0.370
CLV_NRD_NRD_1	150	152	PF00675	0.380
CLV_NRD_NRD_1	235	237	PF00675	0.547
CLV_NRD_NRD_1	345	347	PF00675	0.393
CLV_NRD_NRD_1	389	391	PF00675	0.372
CLV_NRD_NRD_1	411	413	PF00675	0.468
CLV_PCSK_KEX2_1	103	105	PF00082	0.377
CLV_PCSK_KEX2_1	131	133	PF00082	0.370
CLV_PCSK_KEX2_1	235	237	PF00082	0.557
CLV_PCSK_KEX2_1	345	347	PF00082	0.435
CLV_PCSK_KEX2_1	358	360	PF00082	0.359
CLV_PCSK_KEX2_1	389	391	PF00082	0.372
CLV_PCSK_KEX2_1	411	413	PF00082	0.468
CLV_PCSK_PC1ET2_1	358	360	PF00082	0.439
CLV_PCSK_PC7_1	231	237	PF00082	0.474
CLV_PCSK_SKI1_1	20	24	PF00082	0.400
CLV_PCSK_SKI1_1	348	352	PF00082	0.412
CLV_PCSK_SKI1_1	355	359	PF00082	0.387
CLV_PCSK_SKI1_1	40	44	PF00082	0.636
CLV_PCSK_SKI1_1	412	416	PF00082	0.385
DEG_SPOP_SBC_1	213	217	PF00917	0.408
DOC_CYCLIN_yCln2_LP_2	111	117	PF00134	0.470
DOC_CYCLIN_yCln2_LP_2	164	170	PF00134	0.400
DOC_MAPK_gen_1	151	157	PF00069	0.328
DOC_MAPK_gen_1	355	364	PF00069	0.404
DOC_MAPK_gen_1	389	397	PF00069	0.430
DOC_MAPK_MEF2A_6	299	307	PF00069	0.344
DOC_MAPK_MEF2A_6	389	397	PF00069	0.470
DOC_PP1_RVXF_1	302	308	PF00149	0.325
DOC_PP1_RVXF_1	468	474	PF00149	0.528
DOC_PP2B_LxvP_1	155	158	PF13499	0.487
DOC_USP7_MATH_1	158	162	PF00917	0.382
DOC_USP7_MATH_1	240	244	PF00917	0.634
DOC_USP7_MATH_1	250	254	PF00917	0.491
DOC_USP7_MATH_1	271	275	PF00917	0.494
DOC_USP7_MATH_1	85	89	PF00917	0.573
DOC_USP7_UBL2_3	476	480	PF12436	0.436
DOC_WW_Pin1_4	110	115	PF00397	0.424
DOC_WW_Pin1_4	181	186	PF00397	0.466
DOC_WW_Pin1_4	443	448	PF00397	0.526
LIG_14-3-3_CanoR_1	265	270	PF00244	0.416
LIG_14-3-3_CanoR_1	359	363	PF00244	0.442
LIG_14-3-3_CanoR_1	423	429	PF00244	0.581
LIG_Actin_WH2_2	398	413	PF00022	0.466
LIG_BRCT_BRCA1_1	58	62	PF00533	0.340
LIG_FHA_1	106	112	PF00498	0.393
LIG_FHA_1	144	150	PF00498	0.410
LIG_FHA_1	152	158	PF00498	0.373
LIG_FHA_1	21	27	PF00498	0.392
LIG_FHA_1	259	265	PF00498	0.419
LIG_FHA_1	359	365	PF00498	0.388
LIG_FHA_1	381	387	PF00498	0.517
LIG_FHA_1	466	472	PF00498	0.521
LIG_FHA_1	72	78	PF00498	0.485
LIG_FHA_2	282	288	PF00498	0.424
LIG_FHA_2	349	355	PF00498	0.493
LIG_FHA_2	440	446	PF00498	0.486
LIG_LIR_Gen_1	165	175	PF02991	0.364
LIG_LIR_Gen_1	2	12	PF02991	0.431
LIG_LIR_Gen_1	337	343	PF02991	0.508
LIG_LIR_Gen_1	396	405	PF02991	0.355
LIG_LIR_Gen_1	481	489	PF02991	0.490
LIG_LIR_Gen_1	49	60	PF02991	0.331
LIG_LIR_Nem_3	165	171	PF02991	0.377
LIG_LIR_Nem_3	184	189	PF02991	0.347
LIG_LIR_Nem_3	2	8	PF02991	0.486
LIG_LIR_Nem_3	337	342	PF02991	0.508
LIG_LIR_Nem_3	396	401	PF02991	0.337
LIG_LIR_Nem_3	481	485	PF02991	0.493
LIG_LIR_Nem_3	49	55	PF02991	0.348
LIG_PCNA_yPIPBox_3	142	156	PF02747	0.402
LIG_SH2_CRK	168	172	PF00017	0.455
LIG_SH2_CRK	52	56	PF00017	0.418
LIG_SH2_STAP1	52	56	PF00017	0.406
LIG_SH2_STAT5	168	171	PF00017	0.368
LIG_SH2_STAT5	189	192	PF00017	0.499
LIG_SH2_STAT5	292	295	PF00017	0.462
LIG_SH2_STAT5	306	309	PF00017	0.217
LIG_SH3_3	124	130	PF00018	0.415
LIG_SH3_3	179	185	PF00018	0.491
LIG_SH3_3	257	263	PF00018	0.462
LIG_SH3_3	381	387	PF00018	0.515
LIG_SH3_3	57	63	PF00018	0.322
LIG_SUMO_SIM_anti_2	337	344	PF11976	0.511
LIG_SUMO_SIM_par_1	265	270	PF11976	0.334
LIG_SUMO_SIM_par_1	360	366	PF11976	0.465
LIG_SUMO_SIM_par_1	412	417	PF11976	0.438
LIG_SUMO_SIM_par_1	95	100	PF11976	0.503
LIG_TYR_ITIM	50	55	PF00017	0.448
MOD_CK1_1	46	52	PF00069	0.491
MOD_CK2_1	281	287	PF00069	0.405
MOD_CK2_1	348	354	PF00069	0.492
MOD_CK2_1	439	445	PF00069	0.441
MOD_CK2_1	475	481	PF00069	0.483
MOD_GlcNHglycan	242	245	PF01048	0.579
MOD_GlcNHglycan	416	419	PF01048	0.397
MOD_GlcNHglycan	430	433	PF01048	0.475
MOD_GlcNHglycan	78	81	PF01048	0.602
MOD_GSK3_1	105	112	PF00069	0.245
MOD_GSK3_1	156	163	PF00069	0.389
MOD_GSK3_1	177	184	PF00069	0.469
MOD_GSK3_1	273	280	PF00069	0.332
MOD_GSK3_1	344	351	PF00069	0.498
MOD_GSK3_1	376	383	PF00069	0.536
MOD_GSK3_1	439	446	PF00069	0.491
MOD_GSK3_1	85	92	PF00069	0.638
MOD_N-GLC_1	46	51	PF02516	0.558
MOD_NEK2_1	22	27	PF00069	0.410
MOD_NEK2_1	331	336	PF00069	0.474
MOD_NEK2_1	56	61	PF00069	0.342
MOD_NEK2_1	97	102	PF00069	0.476
MOD_NEK2_2	475	480	PF00069	0.479
MOD_PKA_1	151	157	PF00069	0.328
MOD_PKA_1	194	200	PF00069	0.437
MOD_PKA_1	358	364	PF00069	0.494
MOD_PKA_2	344	350	PF00069	0.513
MOD_PKA_2	358	364	PF00069	0.494
MOD_PKA_2	422	428	PF00069	0.570
MOD_Plk_1	46	52	PF00069	0.378
MOD_Plk_2-3	363	369	PF00069	0.435
MOD_Plk_4	14	20	PF00069	0.379
MOD_Plk_4	22	28	PF00069	0.406
MOD_Plk_4	298	304	PF00069	0.453
MOD_Plk_4	338	344	PF00069	0.499
MOD_Plk_4	380	386	PF00069	0.567
MOD_Plk_4	481	487	PF00069	0.398
MOD_Plk_4	56	62	PF00069	0.342
MOD_ProDKin_1	110	116	PF00069	0.430
MOD_ProDKin_1	181	187	PF00069	0.465
MOD_ProDKin_1	443	449	PF00069	0.523
MOD_SUMO_for_1	202	205	PF00179	0.462
TRG_DiLeu_BaEn_1	481	486	PF01217	0.479
TRG_ENDOCYTIC_2	168	171	PF00928	0.368
TRG_ENDOCYTIC_2	52	55	PF00928	0.393
TRG_ER_diArg_1	102	104	PF00400	0.427
TRG_ER_diArg_1	130	132	PF00400	0.388
TRG_ER_diArg_1	251	254	PF00400	0.601
TRG_ER_diArg_1	388	390	PF00400	0.363
TRG_ER_diArg_1	401	404	PF00400	0.353
TRG_ER_diArg_1	410	412	PF00400	0.450
TRG_NLS_MonoExtN_4	37	44	PF00514	0.592
TRG_Pf-PMV_PEXEL_1	254	258	PF00026	0.535
TRG_Pf-PMV_PEXEL_1	325	330	PF00026	0.444

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I1V1	Leptomonas seymouri	73%	99%
A0A0S4J566	Bodo saltans	41%	100%
A0A1X0NTW0	Trypanosomatidae	42%	100%
A0A3S7WTE7	Leishmania donovani	96%	100%
A0A422NRG2	Trypanosoma rangeli	44%	100%
A4H839	Leishmania braziliensis	83%	99%
A4HWG6	Leishmania infantum	95%	100%
C9ZXY1	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	44%	100%
E9AQ67	Leishmania mexicana (strain MHOM/GT/2001/U1103)	93%	100%
P53960	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	28%	100%
Q08B09	Xenopus laevis	29%	100%
Q3THG9	Mus musculus	32%	100%
Q5XI97	Rattus norvegicus	30%	100%
Q6DEJ5	Danio rerio	30%	100%
Q7ZYJ9	Xenopus laevis	28%	100%
Q9BTE6	Homo sapiens	30%	100%