Protein kinase domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

NAK family pseudokinase, putative

Species:

Leishmania major

UniProt:

Q4QFA8_LEIMA

TriTrypDb:

LmjF.15.0770 , LMJLV39_150013800 , LMJSD75_150014000

Length:

666

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

Q4QFA8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QFA8

Function

Biological processes
Term	Name	Level	Count
GO:0006468	protein phosphorylation	5	9
GO:0006793	phosphorus metabolic process	3	9
GO:0006796	phosphate-containing compound metabolic process	4	9
GO:0006807	nitrogen compound metabolic process	2	9
GO:0008152	metabolic process	1	9
GO:0009987	cellular process	1	9
GO:0016310	phosphorylation	5	9
GO:0019538	protein metabolic process	3	9
GO:0036211	protein modification process	4	9
GO:0043170	macromolecule metabolic process	3	9
GO:0043412	macromolecule modification	4	9
GO:0044237	cellular metabolic process	2	9
GO:0044238	primary metabolic process	2	9
GO:0071704	organic substance metabolic process	2	9
GO:1901564	organonitrogen compound metabolic process	3	9

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	11
GO:0003824	catalytic activity	1	11
GO:0004672	protein kinase activity	3	11
GO:0004674	protein serine/threonine kinase activity	4	2
GO:0004713	protein tyrosine kinase activity	4	4
GO:0005488	binding	1	11
GO:0005524	ATP binding	5	11
GO:0016301	kinase activity	4	11
GO:0016740	transferase activity	2	11
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	11
GO:0016773	phosphotransferase activity, alcohol group as acceptor	4	11
GO:0017076	purine nucleotide binding	4	11
GO:0030554	adenyl nucleotide binding	5	11
GO:0032553	ribonucleotide binding	3	11
GO:0032555	purine ribonucleotide binding	4	11
GO:0032559	adenyl ribonucleotide binding	5	11
GO:0035639	purine ribonucleoside triphosphate binding	4	11
GO:0036094	small molecule binding	2	11
GO:0043167	ion binding	2	11
GO:0043168	anion binding	3	11
GO:0097159	organic cyclic compound binding	2	11
GO:0097367	carbohydrate derivative binding	2	11
GO:0140096	catalytic activity, acting on a protein	2	11
GO:1901265	nucleoside phosphate binding	3	11
GO:1901363	heterocyclic compound binding	2	11

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	388	392	PF00656	0.677
CLV_NRD_NRD_1	15	17	PF00675	0.721
CLV_NRD_NRD_1	462	464	PF00675	0.671
CLV_PCSK_KEX2_1	15	17	PF00082	0.712
CLV_PCSK_KEX2_1	261	263	PF00082	0.335
CLV_PCSK_KEX2_1	462	464	PF00082	0.612
CLV_PCSK_KEX2_1	657	659	PF00082	0.671
CLV_PCSK_KEX2_1	94	96	PF00082	0.335
CLV_PCSK_PC1ET2_1	261	263	PF00082	0.335
CLV_PCSK_PC1ET2_1	657	659	PF00082	0.671
CLV_PCSK_PC1ET2_1	94	96	PF00082	0.335
CLV_PCSK_PC7_1	653	659	PF00082	0.685
CLV_PCSK_SKI1_1	313	317	PF00082	0.252
CLV_PCSK_SKI1_1	462	466	PF00082	0.590
CLV_PCSK_SKI1_1	496	500	PF00082	0.755
CLV_PCSK_SKI1_1	534	538	PF00082	0.743
CLV_PCSK_SKI1_1	95	99	PF00082	0.335
DEG_Nend_UBRbox_3	1	3	PF02207	0.761
DOC_CYCLIN_RxL_1	83	96	PF00134	0.402
DOC_MAPK_gen_1	194	203	PF00069	0.447
DOC_MAPK_gen_1	448	456	PF00069	0.569
DOC_MAPK_MEF2A_6	131	140	PF00069	0.355
DOC_PP4_FxxP_1	249	252	PF00568	0.378
DOC_PP4_FxxP_1	627	630	PF00568	0.743
DOC_USP7_MATH_1	176	180	PF00917	0.355
DOC_USP7_MATH_1	304	308	PF00917	0.344
DOC_USP7_MATH_1	478	482	PF00917	0.818
DOC_WW_Pin1_4	306	311	PF00397	0.246
DOC_WW_Pin1_4	369	374	PF00397	0.770
DOC_WW_Pin1_4	470	475	PF00397	0.749
DOC_WW_Pin1_4	504	509	PF00397	0.694
DOC_WW_Pin1_4	514	519	PF00397	0.740
LIG_14-3-3_CanoR_1	152	162	PF00244	0.416
LIG_14-3-3_CanoR_1	164	170	PF00244	0.386
LIG_14-3-3_CanoR_1	313	322	PF00244	0.360
LIG_14-3-3_CanoR_1	653	661	PF00244	0.706
LIG_AP2alpha_1	499	503	PF02296	0.654
LIG_AP2alpha_1	661	665	PF02296	0.670
LIG_AP2alpha_2	256	258	PF02296	0.447
LIG_AP2alpha_2	659	661	PF02296	0.603
LIG_BRCT_BRCA1_1	282	286	PF00533	0.301
LIG_BRCT_BRCA1_1	539	543	PF00533	0.794
LIG_deltaCOP1_diTrp_1	295	300	PF00928	0.435
LIG_deltaCOP1_diTrp_1	356	361	PF00928	0.580
LIG_FHA_1	157	163	PF00498	0.377
LIG_FHA_1	176	182	PF00498	0.351
LIG_FHA_1	288	294	PF00498	0.293
LIG_FHA_1	307	313	PF00498	0.362
LIG_FHA_1	314	320	PF00498	0.335
LIG_FHA_1	623	629	PF00498	0.769
LIG_FHA_1	79	85	PF00498	0.434
LIG_FHA_2	164	170	PF00498	0.447
LIG_FHA_2	190	196	PF00498	0.434
LIG_FHA_2	228	234	PF00498	0.447
LIG_FHA_2	351	357	PF00498	0.599
LIG_Integrin_isoDGR_2	416	418	PF01839	0.706
LIG_LIR_Apic_2	381	385	PF02991	0.804
LIG_LIR_Apic_2	625	630	PF02991	0.748
LIG_LIR_Gen_1	326	336	PF02991	0.399
LIG_LIR_Gen_1	497	506	PF02991	0.682
LIG_LIR_Gen_1	659	665	PF02991	0.609
LIG_LIR_Gen_1	72	78	PF02991	0.340
LIG_LIR_Nem_3	326	331	PF02991	0.387
LIG_LIR_Nem_3	497	502	PF02991	0.685
LIG_LIR_Nem_3	659	664	PF02991	0.607
LIG_LIR_Nem_3	72	77	PF02991	0.356
LIG_LIR_Nem_3	96	100	PF02991	0.355
LIG_LYPXL_yS_3	554	557	PF13949	0.723
LIG_MAD2	367	375	PF02301	0.671
LIG_MYND_1	548	552	PF01753	0.666
LIG_MYND_1	609	613	PF01753	0.545
LIG_Pex14_1	296	300	PF04695	0.447
LIG_Pex14_2	499	503	PF04695	0.654
LIG_Pex14_2	661	665	PF04695	0.621
LIG_PTAP_UEV_1	521	526	PF05743	0.771
LIG_PTB_Apo_2	243	250	PF02174	0.434
LIG_SH2_CRK	150	154	PF00017	0.378
LIG_SH2_CRK	281	285	PF00017	0.368
LIG_SH2_CRK	607	611	PF00017	0.788
LIG_SH2_NCK_1	382	386	PF00017	0.743
LIG_SH2_PTP2	74	77	PF00017	0.355
LIG_SH2_SRC	183	186	PF00017	0.342
LIG_SH2_SRC	607	610	PF00017	0.783
LIG_SH2_STAT3	449	452	PF00017	0.642
LIG_SH2_STAT3	584	587	PF00017	0.805
LIG_SH2_STAT5	144	147	PF00017	0.335
LIG_SH2_STAT5	183	186	PF00017	0.322
LIG_SH2_STAT5	308	311	PF00017	0.335
LIG_SH2_STAT5	74	77	PF00017	0.355
LIG_SH3_1	607	613	PF00018	0.708
LIG_SH3_3	282	288	PF00018	0.447
LIG_SH3_3	372	378	PF00018	0.659
LIG_SH3_3	472	478	PF00018	0.777
LIG_SH3_3	50	56	PF00018	0.540
LIG_SH3_3	519	525	PF00018	0.775
LIG_SH3_3	549	555	PF00018	0.714
LIG_SH3_3	606	612	PF00018	0.704
LIG_SH3_3	82	88	PF00018	0.408
LIG_SUMO_SIM_anti_2	109	114	PF11976	0.378
LIG_TRAF2_1	230	233	PF00917	0.447
LIG_TRAF2_1	427	430	PF00917	0.697
LIG_WRC_WIRS_1	347	352	PF05994	0.542
LIG_WW_1	54	57	PF00397	0.646
LIG_WW_2	609	612	PF00397	0.546
MOD_CDK_SPxxK_3	306	313	PF00069	0.246
MOD_CDK_SPxxK_3	369	376	PF00069	0.544
MOD_CK1_1	127	133	PF00069	0.365
MOD_CK1_1	156	162	PF00069	0.459
MOD_CK1_1	302	308	PF00069	0.430
MOD_CK1_1	473	479	PF00069	0.668
MOD_CK1_1	514	520	PF00069	0.603
MOD_CK1_1	7	13	PF00069	0.649
MOD_CK2_1	111	117	PF00069	0.485
MOD_CK2_1	127	133	PF00069	0.390
MOD_CK2_1	163	169	PF00069	0.447
MOD_CK2_1	227	233	PF00069	0.468
MOD_CK2_1	350	356	PF00069	0.578
MOD_CK2_1	378	384	PF00069	0.721
MOD_GlcNHglycan	155	158	PF01048	0.391
MOD_GlcNHglycan	178	181	PF01048	0.378
MOD_GlcNHglycan	20	23	PF01048	0.696
MOD_GlcNHglycan	419	422	PF01048	0.643
MOD_GlcNHglycan	480	484	PF01048	0.794
MOD_GlcNHglycan	504	507	PF01048	0.750
MOD_GlcNHglycan	522	525	PF01048	0.815
MOD_GlcNHglycan	537	540	PF01048	0.698
MOD_GlcNHglycan	545	548	PF01048	0.667
MOD_GlcNHglycan	573	576	PF01048	0.698
MOD_GlcNHglycan	580	583	PF01048	0.721
MOD_GlcNHglycan	631	634	PF01048	0.782
MOD_GlcNHglycan	9	12	PF01048	0.672
MOD_GSK3_1	152	159	PF00069	0.459
MOD_GSK3_1	264	271	PF00069	0.262
MOD_GSK3_1	302	309	PF00069	0.437
MOD_GSK3_1	346	353	PF00069	0.547
MOD_GSK3_1	502	509	PF00069	0.727
MOD_GSK3_1	510	517	PF00069	0.743
MOD_GSK3_1	537	544	PF00069	0.741
MOD_GSK3_1	57	64	PF00069	0.571
MOD_GSK3_1	644	651	PF00069	0.782
MOD_N-GLC_1	219	224	PF02516	0.441
MOD_N-GLC_1	79	84	PF02516	0.334
MOD_NEK2_1	136	141	PF00069	0.335
MOD_NEK2_1	175	180	PF00069	0.356
MOD_NEK2_1	280	285	PF00069	0.304
MOD_NEK2_1	350	355	PF00069	0.563
MOD_NEK2_1	4	9	PF00069	0.698
MOD_NEK2_1	502	507	PF00069	0.730
MOD_NEK2_1	542	547	PF00069	0.734
MOD_NEK2_1	93	98	PF00069	0.378
MOD_PIKK_1	511	517	PF00454	0.765
MOD_PIKK_1	638	644	PF00454	0.724
MOD_PKA_2	153	159	PF00069	0.438
MOD_PKA_2	163	169	PF00069	0.360
MOD_PKA_2	417	423	PF00069	0.710
MOD_PKA_2	62	68	PF00069	0.486
MOD_PKA_2	652	658	PF00069	0.793
MOD_Plk_1	79	85	PF00069	0.324
MOD_Plk_4	288	294	PF00069	0.246
MOD_Plk_4	33	39	PF00069	0.449
MOD_Plk_4	506	512	PF00069	0.659
MOD_Plk_4	70	76	PF00069	0.378
MOD_ProDKin_1	306	312	PF00069	0.246
MOD_ProDKin_1	369	375	PF00069	0.768
MOD_ProDKin_1	470	476	PF00069	0.753
MOD_ProDKin_1	504	510	PF00069	0.695
MOD_ProDKin_1	514	520	PF00069	0.757
MOD_SUMO_rev_2	111	121	PF00179	0.450
MOD_SUMO_rev_2	359	369	PF00179	0.587
TRG_ENDOCYTIC_2	150	153	PF00928	0.378
TRG_ENDOCYTIC_2	277	280	PF00928	0.335
TRG_ENDOCYTIC_2	281	284	PF00928	0.335
TRG_ENDOCYTIC_2	336	339	PF00928	0.554
TRG_ENDOCYTIC_2	554	557	PF00928	0.740
TRG_ENDOCYTIC_2	74	77	PF00928	0.376
TRG_ER_diArg_1	461	463	PF00400	0.715
TRG_NLS_MonoExtC_3	259	265	PF00514	0.447
TRG_NLS_MonoExtN_4	257	264	PF00514	0.447
TRG_Pf-PMV_PEXEL_1	313	317	PF00026	0.246
TRG_Pf-PMV_PEXEL_1	393	398	PF00026	0.691

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I7R8	Leptomonas seymouri	66%	95%
A0A1X0NUC1	Trypanosomatidae	45%	88%
A0A3S7WTG9	Leishmania donovani	90%	100%
A0A422NRC0	Trypanosoma rangeli	41%	98%
A4H848	Leishmania braziliensis	65%	99%
A4HWH3	Leishmania infantum	90%	100%
C9ZXX7	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	39%	100%
E9AQ75	Leishmania mexicana (strain MHOM/GT/2001/U1103)	82%	100%
V5BK24	Trypanosoma cruzi	41%	98%