Ubiquitin-like modifier-activating enzyme 5

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

E1-like ubiquitin-activating enzyme, putative

Species:

Leishmania major

UniProt:

Q4QF87_LEIMA

TriTrypDb:

LmjF.15.0970 , LMJLV39_150016700 , LMJSD75_150016700

Length:

410

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 25

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	2
GO:0005739	mitochondrion	5	2
GO:0005829	cytosol	2	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

Q4QF87

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QF87

Function

Biological processes
Term	Name	Level	Count
GO:0006807	nitrogen compound metabolic process	2	2
GO:0008152	metabolic process	1	2
GO:0019538	protein metabolic process	3	2
GO:0032446	protein modification by small protein conjugation	6	2
GO:0036211	protein modification process	4	2
GO:0043170	macromolecule metabolic process	3	2
GO:0043412	macromolecule modification	4	2
GO:0044238	primary metabolic process	2	2
GO:0070647	protein modification by small protein conjugation or removal	5	2
GO:0071569	protein ufmylation	7	2
GO:0071704	organic substance metabolic process	2	2
GO:1901564	organonitrogen compound metabolic process	3	2

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	12
GO:0005488	binding	1	11
GO:0005515	protein binding	2	2
GO:0008641	ubiquitin-like modifier activating enzyme activity	2	12
GO:0016874	ligase activity	2	12
GO:0016877	ligase activity, forming carbon-sulfur bonds	3	12
GO:0032182	ubiquitin-like protein binding	3	2
GO:0043167	ion binding	2	11
GO:0043169	cation binding	3	11
GO:0046872	metal ion binding	4	11
GO:0071566	UFM1 activating enzyme activity	3	2
GO:0140096	catalytic activity, acting on a protein	2	12
GO:0140657	ATP-dependent activity	1	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	332	336	PF00656	0.674
CLV_C14_Caspase3-7	378	382	PF00656	0.722
CLV_NRD_NRD_1	299	301	PF00675	0.463
CLV_PCSK_KEX2_1	10	12	PF00082	0.569
CLV_PCSK_KEX2_1	212	214	PF00082	0.310
CLV_PCSK_KEX2_1	277	279	PF00082	0.382
CLV_PCSK_PC1ET2_1	10	12	PF00082	0.569
CLV_PCSK_PC1ET2_1	212	214	PF00082	0.310
CLV_PCSK_PC1ET2_1	277	279	PF00082	0.311
CLV_PCSK_SKI1_1	271	275	PF00082	0.469
CLV_PCSK_SKI1_1	390	394	PF00082	0.718
CLV_PCSK_SKI1_1	68	72	PF00082	0.326
DEG_SCF_FBW7_2	190	197	PF00400	0.326
DEG_SPOP_SBC_1	2	6	PF00917	0.640
DOC_CYCLIN_RxL_1	63	76	PF00134	0.310
DOC_MAPK_MEF2A_6	256	264	PF00069	0.326
DOC_PP1_RVXF_1	66	73	PF00149	0.310
DOC_PP2B_LxvP_1	187	190	PF13499	0.310
DOC_PP4_FxxP_1	86	89	PF00568	0.329
DOC_USP7_MATH_1	2	6	PF00917	0.640
DOC_USP7_MATH_1	338	342	PF00917	0.661
DOC_USP7_MATH_1	351	355	PF00917	0.655
DOC_USP7_MATH_1	395	399	PF00917	0.640
DOC_WW_Pin1_4	136	141	PF00397	0.361
DOC_WW_Pin1_4	190	195	PF00397	0.315
DOC_WW_Pin1_4	3	8	PF00397	0.663
LIG_14-3-3_CanoR_1	157	161	PF00244	0.310
LIG_Clathr_ClatBox_1	69	73	PF01394	0.402
LIG_deltaCOP1_diTrp_1	124	129	PF00928	0.310
LIG_FHA_1	157	163	PF00498	0.404
LIG_FHA_1	164	170	PF00498	0.411
LIG_FHA_1	222	228	PF00498	0.310
LIG_FHA_2	109	115	PF00498	0.349
LIG_FHA_2	119	125	PF00498	0.261
LIG_FHA_2	376	382	PF00498	0.730
LIG_IBAR_NPY_1	20	22	PF08397	0.424
LIG_LIR_Apic_2	166	170	PF02991	0.402
LIG_LIR_Gen_1	123	133	PF02991	0.311
LIG_LIR_Gen_1	19	28	PF02991	0.329
LIG_LIR_Gen_1	35	45	PF02991	0.326
LIG_LIR_Gen_1	73	82	PF02991	0.327
LIG_LIR_Nem_3	123	129	PF02991	0.311
LIG_LIR_Nem_3	19	25	PF02991	0.329
LIG_LIR_Nem_3	247	252	PF02991	0.310
LIG_LIR_Nem_3	257	262	PF02991	0.310
LIG_LIR_Nem_3	35	40	PF02991	0.326
LIG_LIR_Nem_3	73	77	PF02991	0.327
LIG_PCNA_PIPBox_1	225	234	PF02747	0.345
LIG_PCNA_PIPBox_1	79	88	PF02747	0.439
LIG_SH2_CRK	22	26	PF00017	0.344
LIG_SH2_NCK_1	74	78	PF00017	0.310
LIG_SH2_SRC	373	376	PF00017	0.603
LIG_SH2_STAP1	22	26	PF00017	0.326
LIG_SH2_STAP1	282	286	PF00017	0.496
LIG_SH2_STAT3	239	242	PF00017	0.364
LIG_SH2_STAT5	239	242	PF00017	0.317
LIG_SH2_STAT5	249	252	PF00017	0.340
LIG_SH2_STAT5	391	394	PF00017	0.718
LIG_SH3_3	109	115	PF00018	0.446
LIG_SH3_3	137	143	PF00018	0.384
LIG_SUMO_SIM_anti_2	142	148	PF11976	0.327
LIG_SUMO_SIM_par_1	200	206	PF11976	0.329
LIG_TRAF2_1	398	401	PF00917	0.659
LIG_TYR_ITAM	19	40	PF00017	0.345
LIG_UBA3_1	402	407	PF00899	0.552
MOD_CDK_SPxxK_3	3	10	PF00069	0.655
MOD_CK1_1	139	145	PF00069	0.447
MOD_CK1_1	220	226	PF00069	0.329
MOD_CK1_1	353	359	PF00069	0.734
MOD_CK2_1	108	114	PF00069	0.383
MOD_CK2_1	118	124	PF00069	0.299
MOD_CK2_1	366	372	PF00069	0.695
MOD_CK2_1	395	401	PF00069	0.632
MOD_Cter_Amidation	275	278	PF01082	0.306
MOD_GlcNHglycan	12	15	PF01048	0.582
MOD_GlcNHglycan	173	176	PF01048	0.310
MOD_GlcNHglycan	181	184	PF01048	0.310
MOD_GlcNHglycan	340	343	PF01048	0.670
MOD_GlcNHglycan	353	356	PF01048	0.610
MOD_GlcNHglycan	368	371	PF01048	0.632
MOD_GSK3_1	127	134	PF00069	0.414
MOD_GSK3_1	217	224	PF00069	0.323
MOD_GSK3_1	391	398	PF00069	0.626
MOD_N-GLC_1	118	123	PF02516	0.402
MOD_NEK2_2	233	238	PF00069	0.345
MOD_PKA_1	10	16	PF00069	0.557
MOD_PKA_2	10	16	PF00069	0.611
MOD_PKA_2	127	133	PF00069	0.428
MOD_PKA_2	156	162	PF00069	0.310
MOD_Plk_1	325	331	PF00069	0.602
MOD_Plk_2-3	36	42	PF00069	0.428
MOD_Plk_2-3	73	79	PF00069	0.446
MOD_Plk_4	108	114	PF00069	0.446
MOD_Plk_4	52	58	PF00069	0.326
MOD_ProDKin_1	136	142	PF00069	0.361
MOD_ProDKin_1	190	196	PF00069	0.315
MOD_ProDKin_1	3	9	PF00069	0.659
MOD_SUMO_for_1	211	214	PF00179	0.326
MOD_SUMO_rev_2	257	266	PF00179	0.428
MOD_SUMO_rev_2	326	336	PF00179	0.673
MOD_SUMO_rev_2	386	392	PF00179	0.704
TRG_ENDOCYTIC_2	22	25	PF00928	0.329
TRG_ENDOCYTIC_2	252	255	PF00928	0.310
TRG_ENDOCYTIC_2	37	40	PF00928	0.326
TRG_ENDOCYTIC_2	74	77	PF00928	0.310
TRG_NLS_MonoExtC_3	299	305	PF00514	0.585

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P2F3	Leptomonas seymouri	77%	96%
A0A0N0P8E8	Leptomonas seymouri	25%	90%
A0A0S4JGN9	Bodo saltans	71%	98%
A0A1X0NTY2	Trypanosomatidae	69%	100%
A0A3R7JZ58	Trypanosoma rangeli	70%	100%
A0A3S5H6V3	Leishmania donovani	94%	100%
A0A422NC72	Trypanosoma rangeli	29%	83%
A1A4L8	Bos taurus	27%	90%
A4H870	Leishmania braziliensis	89%	100%
A4HWJ5	Leishmania infantum	94%	100%
A6ZT19	Saccharomyces cerevisiae (strain YJM789)	22%	93%
A7MAZ3	Bos taurus	59%	100%
A8XEQ8	Caenorhabditis briggsae	62%	95%
B0WQV1	Culex quinquefasciatus	47%	100%
B3LSM6	Saccharomyces cerevisiae (strain RM11-1a)	22%	93%
B3NUC9	Drosophila erecta	49%	100%
B4GKQ3	Drosophila persimilis	29%	91%
B4GYC7	Drosophila persimilis	58%	100%
B4IK21	Drosophila sechellia	60%	100%
B4JIY0	Drosophila grimshawi	58%	100%
B4L1K2	Drosophila mojavensis	62%	100%
B4M357	Drosophila virilis	58%	100%
B4NDE5	Drosophila willistoni	59%	100%
B4PYA4	Drosophila yakuba	50%	100%
B4R345	Drosophila simulans	59%	100%
B5DS72	Drosophila pseudoobscura pseudoobscura	29%	91%
B5VK45	Saccharomyces cerevisiae (strain AWRI1631)	22%	93%
C3YZ51	Branchiostoma floridae	49%	100%
C9ZXU3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	67%	100%
D0A640	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	29%	81%
D1GY43	Drosophila ananassae	59%	100%
E9AQ96	Leishmania mexicana (strain MHOM/GT/2001/U1103)	92%	100%
O23034	Arabidopsis thaliana	61%	95%
P38820	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	22%	93%
P91430	Caenorhabditis elegans	61%	98%
Q17DT0	Aedes aegypti	61%	100%
Q29HT0	Drosophila pseudoobscura pseudoobscura	60%	100%
Q29PG5	Drosophila pseudoobscura pseudoobscura	29%	91%
Q3KQ23	Xenopus laevis	61%	100%
Q54C02	Dictyostelium discoideum	61%	100%
Q59WH7	Candida albicans (strain SC5314 / ATCC MYA-2876)	23%	94%
Q5M7A4	Rattus norvegicus	62%	100%
Q5R8X4	Pongo abelii	61%	100%
Q6BHZ2	Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968)	20%	92%
Q6GLG7	Xenopus tropicalis	62%	100%
Q6IVA4	Gallus gallus	60%	100%
Q6K6K7	Oryza sativa subsp. japonica	62%	97%
Q8AWD2	Danio rerio	25%	89%
Q8VE47	Mus musculus	60%	100%
Q9GZZ9	Homo sapiens	61%	100%
Q9VYY3	Drosophila melanogaster	59%	100%
V5BPQ6	Trypanosoma cruzi	66%	100%
X1WER2	Danio rerio	60%	100%