RING-type domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

ERAD-associated E3 ubiquitin-protein ligase HRD1, putative

Species:

Leishmania major

UniProt:

Q4QF39_LEIMA

TriTrypDb:

LmjF.15.1410 , LMJLV39_150021100 , LMJSD75_150021100

Length:

478

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	10
GO:0110165	cellular anatomical entity	1	10

Expansion

Sequence features

Q4QF39

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QF39

Function

Biological processes
Term	Name	Level	Count
GO:0006508	proteolysis	4	2
GO:0006511	ubiquitin-dependent protein catabolic process	7	2
GO:0006807	nitrogen compound metabolic process	2	2
GO:0008152	metabolic process	1	2
GO:0009056	catabolic process	2	2
GO:0009057	macromolecule catabolic process	4	2
GO:0009987	cellular process	1	2
GO:0019538	protein metabolic process	3	2
GO:0019941	modification-dependent protein catabolic process	6	2
GO:0043170	macromolecule metabolic process	3	2
GO:0043632	modification-dependent macromolecule catabolic process	5	2
GO:0044237	cellular metabolic process	2	2
GO:0044238	primary metabolic process	2	2
GO:0044248	cellular catabolic process	3	2
GO:0044260	obsolete cellular macromolecule metabolic process	3	2
GO:0044265	obsolete cellular macromolecule catabolic process	4	2
GO:0051603	proteolysis involved in protein catabolic process	5	2
GO:0071704	organic substance metabolic process	2	2
GO:1901564	organonitrogen compound metabolic process	3	2
GO:1901575	organic substance catabolic process	3	2

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	3
GO:0004842	ubiquitin-protein transferase activity	4	3
GO:0016740	transferase activity	2	3
GO:0019787	ubiquitin-like protein transferase activity	3	3
GO:0061630	ubiquitin protein ligase activity	5	2
GO:0061659	ubiquitin-like protein ligase activity	4	2
GO:0140096	catalytic activity, acting on a protein	2	3
GO:0016746	acyltransferase activity	3	1
GO:0061631	ubiquitin conjugating enzyme activity	5	1
GO:0061650	ubiquitin-like protein conjugating enzyme activity	4	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	274	278	PF00656	0.584
CLV_C14_Caspase3-7	418	422	PF00656	0.636
CLV_NRD_NRD_1	269	271	PF00675	0.365
CLV_PCSK_KEX2_1	269	271	PF00082	0.386
CLV_PCSK_KEX2_1	303	305	PF00082	0.323
CLV_PCSK_KEX2_1	455	457	PF00082	0.441
CLV_PCSK_PC1ET2_1	303	305	PF00082	0.338
CLV_PCSK_PC1ET2_1	455	457	PF00082	0.457
CLV_PCSK_SKI1_1	236	240	PF00082	0.436
DEG_MDM2_SWIB_1	112	119	PF02201	0.423
DEG_SPOP_SBC_1	40	44	PF00917	0.396
DOC_CKS1_1	436	441	PF01111	0.714
DOC_MAPK_DCC_7	236	244	PF00069	0.409
DOC_MAPK_MEF2A_6	236	244	PF00069	0.409
DOC_MAPK_MEF2A_6	462	470	PF00069	0.691
DOC_MAPK_NFAT4_5	236	244	PF00069	0.409
DOC_PP2B_LxvP_1	398	401	PF13499	0.689
DOC_USP7_MATH_1	216	220	PF00917	0.469
DOC_USP7_MATH_1	40	44	PF00917	0.396
DOC_USP7_MATH_1	404	408	PF00917	0.744
DOC_USP7_MATH_1	410	414	PF00917	0.705
DOC_USP7_UBL2_3	448	452	PF12436	0.570
DOC_WW_Pin1_4	367	372	PF00397	0.778
DOC_WW_Pin1_4	421	426	PF00397	0.788
DOC_WW_Pin1_4	435	440	PF00397	0.725
LIG_14-3-3_CanoR_1	126	131	PF00244	0.296
LIG_14-3-3_CanoR_1	192	196	PF00244	0.418
LIG_14-3-3_CanoR_1	204	210	PF00244	0.344
LIG_14-3-3_CanoR_1	270	276	PF00244	0.550
LIG_14-3-3_CanoR_1	4	8	PF00244	0.548
LIG_14-3-3_CanoR_1	427	431	PF00244	0.782
LIG_Actin_WH2_2	177	194	PF00022	0.423
LIG_APCC_ABBA_1	94	99	PF00400	0.625
LIG_BRCT_BRCA1_1	106	110	PF00533	0.408
LIG_BRCT_BRCA1_1	222	226	PF00533	0.354
LIG_BRCT_BRCA1_1	41	45	PF00533	0.246
LIG_FHA_1	157	163	PF00498	0.537
LIG_FHA_1	256	262	PF00498	0.574
LIG_FHA_1	3	9	PF00498	0.534
LIG_FHA_1	300	306	PF00498	0.561
LIG_FHA_1	55	61	PF00498	0.366
LIG_FHA_1	76	82	PF00498	0.585
LIG_FHA_2	162	168	PF00498	0.600
LIG_FHA_2	26	32	PF00498	0.513
LIG_FHA_2	277	283	PF00498	0.593
LIG_FHA_2	377	383	PF00498	0.754
LIG_FHA_2	416	422	PF00498	0.744
LIG_LIR_Gen_1	194	203	PF02991	0.491
LIG_LIR_Gen_1	223	234	PF02991	0.316
LIG_LIR_Gen_1	263	271	PF02991	0.524
LIG_LIR_Gen_1	290	298	PF02991	0.538
LIG_LIR_Nem_3	107	112	PF02991	0.381
LIG_LIR_Nem_3	113	119	PF02991	0.367
LIG_LIR_Nem_3	194	200	PF02991	0.466
LIG_LIR_Nem_3	223	229	PF02991	0.344
LIG_LIR_Nem_3	263	267	PF02991	0.523
LIG_LIR_Nem_3	28	32	PF02991	0.368
LIG_LIR_Nem_3	389	394	PF02991	0.713
LIG_LIR_Nem_3	42	48	PF02991	0.262
LIG_LIR_Nem_3	82	87	PF02991	0.619
LIG_LYPXL_S_1	32	36	PF13949	0.565
LIG_LYPXL_yS_3	33	36	PF13949	0.360
LIG_MLH1_MIPbox_1	106	110	PF16413	0.456
LIG_Pex14_2	108	112	PF04695	0.430
LIG_Pex14_2	197	201	PF04695	0.344
LIG_SH2_CRK	177	181	PF00017	0.398
LIG_SH2_CRK	183	187	PF00017	0.363
LIG_SH2_CRK	230	234	PF00017	0.456
LIG_SH2_GRB2like	25	28	PF00017	0.509
LIG_SH2_SRC	230	233	PF00017	0.456
LIG_SH2_STAP1	177	181	PF00017	0.339
LIG_SH2_STAP1	230	234	PF00017	0.456
LIG_SH2_STAP1	25	29	PF00017	0.402
LIG_SH2_STAP1	293	297	PF00017	0.538
LIG_SH2_STAT3	394	397	PF00017	0.731
LIG_SH2_STAT3	97	100	PF00017	0.635
LIG_SH2_STAT5	10	13	PF00017	0.320
LIG_SH2_STAT5	146	149	PF00017	0.357
LIG_SH2_STAT5	232	235	PF00017	0.304
LIG_SH2_STAT5	328	331	PF00017	0.472
LIG_SH2_STAT5	87	90	PF00017	0.498
LIG_SH3_3	340	346	PF00018	0.661
LIG_SH3_3	89	95	PF00018	0.533
LIG_SUMO_SIM_par_1	183	190	PF11976	0.456
LIG_SUMO_SIM_par_1	46	52	PF11976	0.374
LIG_TRAF2_1	74	77	PF00917	0.599
LIG_TYR_ITIM	181	186	PF00017	0.442
LIG_UBA3_1	157	163	PF00899	0.527
LIG_Vh1_VBS_1	247	265	PF01044	0.536
LIG_WRC_WIRS_1	12	17	PF05994	0.414
LIG_WRC_WIRS_1	81	86	PF05994	0.595
MOD_CDC14_SPxK_1	424	427	PF00782	0.767
MOD_CDK_SPxK_1	421	427	PF00069	0.771
MOD_CK1_1	104	110	PF00069	0.389
MOD_CK1_1	190	196	PF00069	0.385
MOD_CK1_1	403	409	PF00069	0.729
MOD_CK1_1	415	421	PF00069	0.700
MOD_CK1_1	75	81	PF00069	0.557
MOD_CK2_1	161	167	PF00069	0.586
MOD_CK2_1	216	222	PF00069	0.389
MOD_CK2_1	25	31	PF00069	0.505
MOD_GlcNHglycan	103	106	PF01048	0.418
MOD_GlcNHglycan	138	141	PF01048	0.402
MOD_GlcNHglycan	258	261	PF01048	0.409
MOD_GlcNHglycan	340	343	PF01048	0.534
MOD_GlcNHglycan	402	405	PF01048	0.500
MOD_GlcNHglycan	406	409	PF01048	0.511
MOD_GlcNHglycan	414	417	PF01048	0.483
MOD_GlcNHglycan	421	424	PF01048	0.511
MOD_GSK3_1	121	128	PF00069	0.414
MOD_GSK3_1	129	136	PF00069	0.355
MOD_GSK3_1	186	193	PF00069	0.274
MOD_GSK3_1	216	223	PF00069	0.333
MOD_GSK3_1	400	407	PF00069	0.693
MOD_GSK3_1	408	415	PF00069	0.709
MOD_GSK3_1	426	433	PF00069	0.755
MOD_GSK3_1	75	82	PF00069	0.553
MOD_N-GLC_1	40	45	PF02516	0.541
MOD_N-GLC_1	72	77	PF02516	0.341
MOD_NEK2_1	110	115	PF00069	0.378
MOD_NEK2_1	16	21	PF00069	0.325
MOD_NEK2_1	161	166	PF00069	0.544
MOD_NEK2_1	175	180	PF00069	0.327
MOD_NEK2_1	185	190	PF00069	0.297
MOD_NEK2_1	191	196	PF00069	0.209
MOD_NEK2_1	225	230	PF00069	0.333
MOD_NEK2_1	255	260	PF00069	0.544
MOD_NEK2_1	39	44	PF00069	0.343
MOD_NEK2_1	80	85	PF00069	0.538
MOD_PKA_2	125	131	PF00069	0.299
MOD_PKA_2	191	197	PF00069	0.456
MOD_PKA_2	271	277	PF00069	0.593
MOD_PKA_2	287	293	PF00069	0.418
MOD_PKA_2	3	9	PF00069	0.546
MOD_PKA_2	426	432	PF00069	0.745
MOD_Plk_1	40	46	PF00069	0.284
MOD_Plk_1	75	81	PF00069	0.584
MOD_Plk_4	104	110	PF00069	0.331
MOD_Plk_4	11	17	PF00069	0.255
MOD_Plk_4	133	139	PF00069	0.367
MOD_Plk_4	156	162	PF00069	0.522
MOD_Plk_4	175	181	PF00069	0.398
MOD_Plk_4	196	202	PF00069	0.355
MOD_Plk_4	205	211	PF00069	0.390
MOD_Plk_4	220	226	PF00069	0.361
MOD_Plk_4	3	9	PF00069	0.492
MOD_Plk_4	49	55	PF00069	0.284
MOD_ProDKin_1	367	373	PF00069	0.778
MOD_ProDKin_1	421	427	PF00069	0.790
MOD_ProDKin_1	435	441	PF00069	0.718
MOD_SUMO_rev_2	167	174	PF00179	0.646
TRG_DiLeu_BaEn_1	76	81	PF01217	0.581
TRG_DiLeu_BaEn_2	221	227	PF01217	0.423
TRG_DiLeu_BaEn_4	76	82	PF01217	0.608
TRG_DiLeu_BaLyEn_6	237	242	PF01217	0.320
TRG_DiLeu_BaLyEn_6	43	48	PF01217	0.332
TRG_ENDOCYTIC_2	146	149	PF00928	0.346
TRG_ENDOCYTIC_2	177	180	PF00928	0.320
TRG_ENDOCYTIC_2	183	186	PF00928	0.276
TRG_ENDOCYTIC_2	230	233	PF00928	0.427
TRG_ENDOCYTIC_2	29	32	PF00928	0.394
TRG_ENDOCYTIC_2	293	296	PF00928	0.510
TRG_ENDOCYTIC_2	33	36	PF00928	0.348
TRG_ER_diArg_1	269	272	PF00400	0.592
TRG_Pf-PMV_PEXEL_1	455	459	PF00026	0.461
TRG_Pf-PMV_PEXEL_1	462	467	PF00026	0.425

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I9A3	Leptomonas seymouri	48%	89%
A0A1X0NU29	Trypanosomatidae	33%	91%
A0A3R7MBX1	Trypanosoma rangeli	37%	89%
A0A3S5H6W0	Leishmania donovani	95%	100%
A4H8A9	Leishmania braziliensis	77%	97%
A4HWM9	Leishmania infantum	95%	100%
A8Y4B2	Caenorhabditis briggsae	27%	77%
C9ZXQ9	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	31%	98%
E9AQD6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	92%	100%
O74757	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	24%	71%
Q20798	Caenorhabditis elegans	28%	78%
Q5XHH7	Xenopus laevis	27%	80%
Q5Z880	Oryza sativa subsp. japonica	25%	96%
Q6NPT7	Arabidopsis thaliana	27%	100%
Q6NRL6	Xenopus laevis	28%	79%
Q803I8	Danio rerio	27%	76%
Q86TM6	Homo sapiens	27%	77%
Q95SP2	Drosophila melanogaster	29%	76%
Q9DBY1	Mus musculus	27%	78%
Q9LW77	Arabidopsis thaliana	26%	97%
V5BUE9	Trypanosoma cruzi	30%	90%