Putative 3-Beta-hydroxysteroid-delta(8),delta(7)-isomerase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

3-Beta-hydroxysteroid-delta(8), delta(7)-isomerase, putative

Species:

Leishmania major

UniProt:

Q4QEY6_LEIMA

TriTrypDb:

LmjF.16.0340 , LMJLV39_160008600 , LMJSD75_160008600

Length:

169

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 5
NetGPI	no	yes: 0, no: 5

Cellular components
Term	Name	Level	Count
GO:0005783	endoplasmic reticulum	5	2
GO:0016020	membrane	2	6
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	6
GO:0005789	endoplasmic reticulum membrane	4	3
GO:0031090	organelle membrane	3	3

Expansion

Sequence features

Q4QEY6

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QEY6

Function

Biological processes
Term	Name	Level	Count
GO:0006629	lipid metabolic process	3	6
GO:0006694	steroid biosynthetic process	5	2
GO:0008152	metabolic process	1	6
GO:0008202	steroid metabolic process	4	6
GO:0008610	lipid biosynthetic process	4	2
GO:0009058	biosynthetic process	2	2
GO:0016125	sterol metabolic process	4	6
GO:0016126	sterol biosynthetic process	5	2
GO:0044238	primary metabolic process	2	6
GO:0071704	organic substance metabolic process	2	6
GO:1901360	organic cyclic compound metabolic process	3	6
GO:1901362	organic cyclic compound biosynthetic process	4	2
GO:1901576	organic substance biosynthetic process	3	2
GO:1901615	organic hydroxy compound metabolic process	3	6
GO:1901617	organic hydroxy compound biosynthetic process	4	2

Molecular functions
Term	Name	Level	Count
GO:0000247	C-8 sterol isomerase activity	5	2
GO:0003824	catalytic activity	1	6
GO:0004769	steroid delta-isomerase activity	5	2
GO:0016853	isomerase activity	2	6
GO:0016860	intramolecular oxidoreductase activity	3	6
GO:0016863	intramolecular oxidoreductase activity, transposing C=C bonds	4	6
GO:0047750	cholestenol delta-isomerase activity	5	6

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	21	25	PF00656	0.257
CLV_NRD_NRD_1	154	156	PF00675	0.418
CLV_NRD_NRD_1	159	161	PF00675	0.441
CLV_PCSK_KEX2_1	154	156	PF00082	0.384
CLV_PCSK_KEX2_1	159	161	PF00082	0.362
CLV_PCSK_PC7_1	155	161	PF00082	0.384
CLV_PCSK_SKI1_1	68	72	PF00082	0.208
DEG_Nend_Nbox_1	1	3	PF02207	0.347
DOC_CDC14_PxL_1	92	100	PF14671	0.250
DOC_CYCLIN_yCln2_LP_2	126	132	PF00134	0.294
DOC_MAPK_gen_1	159	165	PF00069	0.544
DOC_MAPK_MEF2A_6	50	57	PF00069	0.241
DOC_PP2B_LxvP_1	126	129	PF13499	0.241
DOC_WW_Pin1_4	104	109	PF00397	0.250
LIG_14-3-3_CanoR_1	141	147	PF00244	0.579
LIG_14-3-3_CanoR_1	26	34	PF00244	0.358
LIG_FHA_1	10	16	PF00498	0.436
LIG_FHA_2	19	25	PF00498	0.257
LIG_LIR_Gen_1	17	28	PF02991	0.320
LIG_LIR_Gen_1	72	81	PF02991	0.241
LIG_LIR_Nem_3	136	140	PF02991	0.489
LIG_LIR_Nem_3	17	23	PF02991	0.324
LIG_LIR_Nem_3	72	77	PF02991	0.245
LIG_LIR_Nem_3	78	84	PF02991	0.244
LIG_Pex14_2	112	116	PF04695	0.257
LIG_SH2_CRK	81	85	PF00017	0.241
LIG_SH2_NCK_1	20	24	PF00017	0.312
LIG_SH2_PTP2	137	140	PF00017	0.496
LIG_SH2_STAP1	27	31	PF00017	0.335
LIG_SH2_STAT3	86	89	PF00017	0.241
LIG_SH2_STAT5	113	116	PF00017	0.251
LIG_SH2_STAT5	125	128	PF00017	0.279
LIG_SH2_STAT5	137	140	PF00017	0.496
LIG_SH2_STAT5	20	23	PF00017	0.258
LIG_SH2_STAT5	86	89	PF00017	0.255
LIG_SH3_3	46	52	PF00018	0.321
LIG_SH3_3	60	66	PF00018	0.529
LIG_SUMO_SIM_par_1	40	46	PF11976	0.241
LIG_TRFH_1	125	129	PF08558	0.294
LIG_TYR_ITIM	123	128	PF00017	0.224
LIG_UBA3_1	44	50	PF00899	0.241
LIG_WRC_WIRS_1	31	36	PF05994	0.310
MOD_CK1_1	153	159	PF00069	0.615
MOD_CK2_1	40	46	PF00069	0.241
MOD_Cter_Amidation	157	160	PF01082	0.361
MOD_GlcNHglycan	160	163	PF01048	0.403
MOD_GlcNHglycan	36	39	PF01048	0.509
MOD_GlcNHglycan	92	95	PF01048	0.450
MOD_GSK3_1	14	21	PF00069	0.369
MOD_GSK3_1	26	33	PF00069	0.388
MOD_N-GLC_1	18	23	PF02516	0.463
MOD_N-GLC_1	34	39	PF02516	0.562
MOD_N-GLC_2	142	144	PF02516	0.325
MOD_NEK2_1	34	39	PF00069	0.282
MOD_PIKK_1	75	81	PF00454	0.298
MOD_PKA_2	153	159	PF00069	0.581
MOD_Plk_1	102	108	PF00069	0.250
MOD_Plk_4	40	46	PF00069	0.307
MOD_Plk_4	80	86	PF00069	0.241
MOD_ProDKin_1	104	110	PF00069	0.250
TRG_DiLeu_BaLyEn_6	11	16	PF01217	0.316
TRG_ENDOCYTIC_2	113	116	PF00928	0.241
TRG_ENDOCYTIC_2	125	128	PF00928	0.241
TRG_ENDOCYTIC_2	137	140	PF00928	0.457
TRG_ENDOCYTIC_2	20	23	PF00928	0.279
TRG_ENDOCYTIC_2	58	61	PF00928	0.494
TRG_ENDOCYTIC_2	81	84	PF00928	0.271

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0S4JDK8	Bodo saltans	67%	70%
A0A1X0NZ45	Trypanosomatidae	63%	70%
A4HWT3	Leishmania infantum	92%	100%
E9AQJ1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	89%	100%
Q15125	Homo sapiens	32%	73%