Putative tubulin tyrosine ligase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

tubulin tyrosine ligase, putative

Species:

Leishmania major

UniProt:

Q4QEY2_LEIMA

TriTrypDb:

LmjF.16.0380 , LMJLV39_160009100 , LMJSD75_160009100

Length:

810

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 9

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	2
GO:0005874	microtubule	6	7
GO:0005929	cilium	4	8
GO:0042995	cell projection	2	8
GO:0043226	organelle	2	8
GO:0043227	membrane-bounded organelle	3	8
GO:0099080	supramolecular complex	2	7
GO:0099081	supramolecular polymer	3	7
GO:0099512	supramolecular fiber	4	7
GO:0099513	polymeric cytoskeletal fiber	5	7
GO:0110165	cellular anatomical entity	1	8
GO:0120025	plasma membrane bounded cell projection	3	8

Expansion

Sequence features

Q4QEY2

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QEY2

Function

Biological processes
Term	Name	Level	Count
GO:0000226	microtubule cytoskeleton organization	3	3
GO:0006807	nitrogen compound metabolic process	2	11
GO:0006996	organelle organization	4	3
GO:0007010	cytoskeleton organization	5	3
GO:0007017	microtubule-based process	2	3
GO:0008152	metabolic process	1	11
GO:0009987	cellular process	1	3
GO:0016043	cellular component organization	3	3
GO:0018095	protein polyglutamylation	7	8
GO:0018193	peptidyl-amino acid modification	5	8
GO:0018200	peptidyl-glutamic acid modification	6	8
GO:0019538	protein metabolic process	3	11
GO:0036211	protein modification process	4	11
GO:0043170	macromolecule metabolic process	3	11
GO:0043412	macromolecule modification	4	11
GO:0044238	primary metabolic process	2	11
GO:0071704	organic substance metabolic process	2	11
GO:0071840	cellular component organization or biogenesis	2	3
GO:1901564	organonitrogen compound metabolic process	3	11

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	7
GO:0003824	catalytic activity	1	11
GO:0004835	tubulin-tyrosine ligase activity	3	8
GO:0005488	binding	1	8
GO:0005515	protein binding	2	3
GO:0005524	ATP binding	5	7
GO:0008092	cytoskeletal protein binding	3	3
GO:0015631	tubulin binding	4	3
GO:0016874	ligase activity	2	11
GO:0016879	ligase activity, forming carbon-nitrogen bonds	3	8
GO:0016881	acid-amino acid ligase activity	4	8
GO:0017076	purine nucleotide binding	4	7
GO:0030554	adenyl nucleotide binding	5	7
GO:0032553	ribonucleotide binding	3	7
GO:0032555	purine ribonucleotide binding	4	7
GO:0032559	adenyl ribonucleotide binding	5	7
GO:0035639	purine ribonucleoside triphosphate binding	4	7
GO:0036094	small molecule binding	2	7
GO:0043167	ion binding	2	7
GO:0043168	anion binding	3	7
GO:0070739	protein-glutamic acid ligase activity	3	3
GO:0070740	tubulin-glutamic acid ligase activity	4	3
GO:0097159	organic cyclic compound binding	2	7
GO:0097367	carbohydrate derivative binding	2	7
GO:0140096	catalytic activity, acting on a protein	2	8
GO:1901265	nucleoside phosphate binding	3	7
GO:1901363	heterocyclic compound binding	2	7

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	100	102	PF00675	0.796
CLV_NRD_NRD_1	323	325	PF00675	0.638
CLV_NRD_NRD_1	356	358	PF00675	0.510
CLV_NRD_NRD_1	385	387	PF00675	0.341
CLV_NRD_NRD_1	395	397	PF00675	0.353
CLV_NRD_NRD_1	443	445	PF00675	0.244
CLV_NRD_NRD_1	478	480	PF00675	0.322
CLV_NRD_NRD_1	55	57	PF00675	0.664
CLV_NRD_NRD_1	62	64	PF00675	0.664
CLV_NRD_NRD_1	71	73	PF00675	0.770
CLV_PCSK_FUR_1	321	325	PF00082	0.629
CLV_PCSK_KEX2_1	100	102	PF00082	0.705
CLV_PCSK_KEX2_1	145	147	PF00082	0.590
CLV_PCSK_KEX2_1	196	198	PF00082	0.653
CLV_PCSK_KEX2_1	323	325	PF00082	0.638
CLV_PCSK_KEX2_1	356	358	PF00082	0.495
CLV_PCSK_KEX2_1	395	397	PF00082	0.353
CLV_PCSK_KEX2_1	443	445	PF00082	0.244
CLV_PCSK_KEX2_1	477	479	PF00082	0.322
CLV_PCSK_PC1ET2_1	100	102	PF00082	0.690
CLV_PCSK_PC1ET2_1	145	147	PF00082	0.590
CLV_PCSK_PC1ET2_1	196	198	PF00082	0.642
CLV_PCSK_SKI1_1	14	18	PF00082	0.662
CLV_PCSK_SKI1_1	494	498	PF00082	0.318
CLV_PCSK_SKI1_1	58	62	PF00082	0.580
CLV_PCSK_SKI1_1	760	764	PF00082	0.295
CLV_PCSK_SKI1_1	772	776	PF00082	0.309
CLV_PCSK_SKI1_1	97	101	PF00082	0.646
DEG_Nend_UBRbox_2	1	3	PF02207	0.657
DEG_SCF_FBW7_1	83	89	PF00400	0.701
DEG_SPOP_SBC_1	283	287	PF00917	0.626
DEG_SPOP_SBC_1	362	366	PF00917	0.407
DOC_CKS1_1	83	88	PF01111	0.701
DOC_MAPK_gen_1	196	204	PF00069	0.620
DOC_MAPK_gen_1	443	449	PF00069	0.444
DOC_MAPK_gen_1	56	62	PF00069	0.562
DOC_MAPK_gen_1	612	620	PF00069	0.552
DOC_MAPK_MEF2A_6	612	620	PF00069	0.552
DOC_MAPK_NFAT4_5	613	621	PF00069	0.552
DOC_MAPK_RevD_3	184	197	PF00069	0.610
DOC_PP1_RVXF_1	700	707	PF00149	0.518
DOC_PP1_RVXF_1	770	776	PF00149	0.518
DOC_USP7_MATH_1	165	169	PF00917	0.728
DOC_USP7_MATH_1	189	193	PF00917	0.721
DOC_USP7_MATH_1	208	212	PF00917	0.579
DOC_USP7_MATH_1	216	220	PF00917	0.691
DOC_USP7_MATH_1	229	233	PF00917	0.742
DOC_USP7_MATH_1	274	278	PF00917	0.682
DOC_USP7_MATH_1	303	307	PF00917	0.674
DOC_USP7_MATH_1	313	317	PF00917	0.754
DOC_USP7_MATH_1	41	45	PF00917	0.606
DOC_USP7_MATH_1	431	435	PF00917	0.534
DOC_USP7_MATH_1	544	548	PF00917	0.482
DOC_USP7_MATH_1	577	581	PF00917	0.616
DOC_USP7_MATH_1	586	590	PF00917	0.584
DOC_USP7_MATH_1	646	650	PF00917	0.584
DOC_USP7_MATH_1	748	752	PF00917	0.527
DOC_USP7_UBL2_3	196	200	PF12436	0.530
DOC_USP7_UBL2_3	802	806	PF12436	0.396
DOC_WW_Pin1_4	21	26	PF00397	0.668
DOC_WW_Pin1_4	279	284	PF00397	0.705
DOC_WW_Pin1_4	44	49	PF00397	0.647
DOC_WW_Pin1_4	522	527	PF00397	0.550
DOC_WW_Pin1_4	563	568	PF00397	0.528
DOC_WW_Pin1_4	746	751	PF00397	0.491
DOC_WW_Pin1_4	82	87	PF00397	0.708
DOC_WW_Pin1_4	92	97	PF00397	0.597
LIG_14-3-3_CanoR_1	250	259	PF00244	0.587
LIG_14-3-3_CanoR_1	357	363	PF00244	0.370
LIG_14-3-3_CanoR_1	408	412	PF00244	0.518
LIG_14-3-3_CanoR_1	444	450	PF00244	0.516
LIG_14-3-3_CanoR_1	529	533	PF00244	0.444
LIG_14-3-3_CanoR_1	81	86	PF00244	0.688
LIG_Actin_WH2_2	697	715	PF00022	0.518
LIG_APCC_ABBA_1	468	473	PF00400	0.518
LIG_BIR_III_4	174	178	PF00653	0.661
LIG_BIR_III_4	426	430	PF00653	0.475
LIG_BRCT_BRCA1_1	363	367	PF00533	0.542
LIG_BRCT_BRCA1_1	450	454	PF00533	0.522
LIG_BRCT_BRCA1_1	568	572	PF00533	0.522
LIG_deltaCOP1_diTrp_1	347	352	PF00928	0.482
LIG_eIF4E_1	602	608	PF01652	0.518
LIG_eIF4E_1	729	735	PF01652	0.476
LIG_FHA_1	145	151	PF00498	0.589
LIG_FHA_1	197	203	PF00498	0.656
LIG_FHA_1	413	419	PF00498	0.491
LIG_FHA_1	459	465	PF00498	0.497
LIG_FHA_1	479	485	PF00498	0.518
LIG_FHA_1	573	579	PF00498	0.475
LIG_FHA_1	752	758	PF00498	0.592
LIG_FHA_1	768	774	PF00498	0.487
LIG_FHA_1	87	93	PF00498	0.792
LIG_FHA_2	251	257	PF00498	0.600
LIG_FHA_2	383	389	PF00498	0.518
LIG_FHA_2	411	417	PF00498	0.518
LIG_FHA_2	754	760	PF00498	0.518
LIG_LIR_Apic_2	461	465	PF02991	0.543
LIG_LIR_Apic_2	531	535	PF02991	0.444
LIG_LIR_Gen_1	347	355	PF02991	0.477
LIG_LIR_Gen_1	404	414	PF02991	0.522
LIG_LIR_Gen_1	634	645	PF02991	0.486
LIG_LIR_Gen_1	680	688	PF02991	0.469
LIG_LIR_Nem_3	347	351	PF02991	0.486
LIG_LIR_Nem_3	388	394	PF02991	0.519
LIG_LIR_Nem_3	404	409	PF02991	0.476
LIG_LIR_Nem_3	623	628	PF02991	0.519
LIG_LIR_Nem_3	634	640	PF02991	0.495
LIG_LIR_Nem_3	668	674	PF02991	0.429
LIG_LIR_Nem_3	680	684	PF02991	0.469
LIG_LIR_Nem_3	691	695	PF02991	0.450
LIG_LIR_Nem_3	725	729	PF02991	0.572
LIG_LIR_Nem_3	803	808	PF02991	0.471
LIG_MAD2	772	780	PF02301	0.518
LIG_PCNA_PIPBox_1	652	661	PF02747	0.518
LIG_PDZ_Class_2	805	810	PF00595	0.450
LIG_Pex14_2	471	475	PF04695	0.522
LIG_Pex14_2	681	685	PF04695	0.502
LIG_PTB_Apo_2	700	707	PF02174	0.518
LIG_SH2_CRK	391	395	PF00017	0.521
LIG_SH2_CRK	406	410	PF00017	0.518
LIG_SH2_CRK	462	466	PF00017	0.518
LIG_SH2_CRK	625	629	PF00017	0.517
LIG_SH2_CRK	671	675	PF00017	0.486
LIG_SH2_CRK	709	713	PF00017	0.510
LIG_SH2_CRK	731	735	PF00017	0.518
LIG_SH2_NCK_1	462	466	PF00017	0.518
LIG_SH2_PTP2	619	622	PF00017	0.518
LIG_SH2_SRC	597	600	PF00017	0.491
LIG_SH2_STAP1	731	735	PF00017	0.518
LIG_SH2_STAT5	397	400	PF00017	0.518
LIG_SH2_STAT5	597	600	PF00017	0.552
LIG_SH2_STAT5	602	605	PF00017	0.552
LIG_SH2_STAT5	619	622	PF00017	0.547
LIG_SH2_STAT5	631	634	PF00017	0.457
LIG_SH2_STAT5	687	690	PF00017	0.550
LIG_SH2_STAT5	695	698	PF00017	0.552
LIG_SH3_3	132	138	PF00018	0.573
LIG_SH3_3	183	189	PF00018	0.813
LIG_SH3_3	201	207	PF00018	0.514
LIG_SH3_3	564	570	PF00018	0.516
LIG_SH3_3	771	777	PF00018	0.518
LIG_SH3_3	85	91	PF00018	0.668
LIG_SH3_4	137	144	PF00018	0.569
LIG_SUMO_SIM_anti_2	199	205	PF11976	0.624
LIG_SUMO_SIM_par_1	445	451	PF11976	0.491
LIG_TRAF2_1	139	142	PF00917	0.581
LIG_TYR_ITAM	388	409	PF00017	0.518
LIG_TYR_ITIM	389	394	PF00017	0.518
LIG_TYR_ITIM	707	712	PF00017	0.518
LIG_WRC_WIRS_1	449	454	PF05994	0.522
LIG_WRC_WIRS_1	518	523	PF05994	0.522
LIG_WW_1	776	779	PF00397	0.522
LIG_WW_2	90	93	PF00397	0.604
MOD_CDK_SPK_2	92	97	PF00069	0.568
MOD_CDK_SPxxK_3	522	529	PF00069	0.491
MOD_CK1_1	234	240	PF00069	0.776
MOD_CK1_1	282	288	PF00069	0.694
MOD_CK1_1	306	312	PF00069	0.749
MOD_CK1_1	44	50	PF00069	0.611
MOD_CK1_1	448	454	PF00069	0.491
MOD_CK1_1	548	554	PF00069	0.572
MOD_CK1_1	563	569	PF00069	0.535
MOD_CK1_1	576	582	PF00069	0.498
MOD_CK1_1	593	599	PF00069	0.426
MOD_CK1_1	648	654	PF00069	0.522
MOD_CK1_1	657	663	PF00069	0.498
MOD_CK1_1	751	757	PF00069	0.527
MOD_CK1_1	82	88	PF00069	0.697
MOD_CK1_1	95	101	PF00069	0.700
MOD_CK2_1	544	550	PF00069	0.597
MOD_Cter_Amidation	194	197	PF01082	0.651
MOD_Cter_Amidation	610	613	PF01082	0.353
MOD_GlcNHglycan	105	108	PF01048	0.689
MOD_GlcNHglycan	119	122	PF01048	0.612
MOD_GlcNHglycan	18	21	PF01048	0.673
MOD_GlcNHglycan	182	185	PF01048	0.651
MOD_GlcNHglycan	210	213	PF01048	0.713
MOD_GlcNHglycan	218	221	PF01048	0.760
MOD_GlcNHglycan	237	240	PF01048	0.761
MOD_GlcNHglycan	276	279	PF01048	0.796
MOD_GlcNHglycan	295	298	PF01048	0.784
MOD_GlcNHglycan	315	318	PF01048	0.661
MOD_GlcNHglycan	418	421	PF01048	0.293
MOD_GlcNHglycan	433	436	PF01048	0.369
MOD_GlcNHglycan	550	553	PF01048	0.391
MOD_GlcNHglycan	556	559	PF01048	0.333
MOD_GlcNHglycan	575	578	PF01048	0.346
MOD_GlcNHglycan	588	591	PF01048	0.299
MOD_GlcNHglycan	8	11	PF01048	0.828
MOD_GlcNHglycan	97	100	PF01048	0.793
MOD_GSK3_1	165	172	PF00069	0.766
MOD_GSK3_1	2	9	PF00069	0.654
MOD_GSK3_1	231	238	PF00069	0.748
MOD_GSK3_1	269	276	PF00069	0.720
MOD_GSK3_1	278	285	PF00069	0.741
MOD_GSK3_1	302	309	PF00069	0.674
MOD_GSK3_1	358	365	PF00069	0.503
MOD_GSK3_1	412	419	PF00069	0.536
MOD_GSK3_1	43	50	PF00069	0.696
MOD_GSK3_1	454	461	PF00069	0.518
MOD_GSK3_1	517	524	PF00069	0.520
MOD_GSK3_1	544	551	PF00069	0.560
MOD_GSK3_1	572	579	PF00069	0.567
MOD_GSK3_1	586	593	PF00069	0.528
MOD_GSK3_1	597	604	PF00069	0.486
MOD_GSK3_1	71	78	PF00069	0.689
MOD_GSK3_1	746	753	PF00069	0.518
MOD_GSK3_1	792	799	PF00069	0.463
MOD_GSK3_1	82	89	PF00069	0.738
MOD_N-GLC_1	208	213	PF02516	0.652
MOD_N-GLC_1	21	26	PF02516	0.569
MOD_N-GLC_1	572	577	PF02516	0.275
MOD_N-GLC_1	746	751	PF02516	0.318
MOD_N-GLC_2	560	562	PF02516	0.291
MOD_NEK2_1	235	240	PF00069	0.578
MOD_NEK2_1	284	289	PF00069	0.648
MOD_NEK2_1	454	459	PF00069	0.518
MOD_NEK2_1	521	526	PF00069	0.525
MOD_NEK2_1	572	577	PF00069	0.532
MOD_NEK2_1	621	626	PF00069	0.481
MOD_NEK2_1	640	645	PF00069	0.518
MOD_NEK2_1	752	757	PF00069	0.518
MOD_NEK2_1	767	772	PF00069	0.518
MOD_NEK2_2	245	250	PF00069	0.608
MOD_NEK2_2	363	368	PF00069	0.485
MOD_NEK2_2	407	412	PF00069	0.518
MOD_PIKK_1	315	321	PF00454	0.676
MOD_PIKK_1	478	484	PF00454	0.482
MOD_PIKK_1	488	494	PF00454	0.489
MOD_PIKK_1	577	583	PF00454	0.491
MOD_PIKK_1	648	654	PF00454	0.429
MOD_PKA_1	196	202	PF00069	0.653
MOD_PKA_1	478	484	PF00069	0.522
MOD_PKA_1	512	518	PF00069	0.502
MOD_PKA_1	58	64	PF00069	0.652
MOD_PKA_2	196	202	PF00069	0.653
MOD_PKA_2	306	312	PF00069	0.703
MOD_PKA_2	358	364	PF00069	0.433
MOD_PKA_2	407	413	PF00069	0.518
MOD_PKA_2	478	484	PF00069	0.518
MOD_PKA_2	528	534	PF00069	0.465
MOD_PKA_2	71	77	PF00069	0.831
MOD_PKB_1	77	85	PF00069	0.663
MOD_Plk_1	245	251	PF00069	0.622
MOD_Plk_1	466	472	PF00069	0.466
MOD_Plk_1	79	85	PF00069	0.644
MOD_Plk_2-3	131	137	PF00069	0.646
MOD_Plk_2-3	722	728	PF00069	0.523
MOD_Plk_4	466	472	PF00069	0.491
MOD_Plk_4	517	523	PF00069	0.522
MOD_Plk_4	528	534	PF00069	0.442
MOD_Plk_4	593	599	PF00069	0.526
MOD_ProDKin_1	21	27	PF00069	0.665
MOD_ProDKin_1	279	285	PF00069	0.702
MOD_ProDKin_1	44	50	PF00069	0.648
MOD_ProDKin_1	522	528	PF00069	0.550
MOD_ProDKin_1	563	569	PF00069	0.528
MOD_ProDKin_1	746	752	PF00069	0.491
MOD_ProDKin_1	82	88	PF00069	0.701
MOD_ProDKin_1	92	98	PF00069	0.599
MOD_SUMO_for_1	126	129	PF00179	0.620
MOD_SUMO_rev_2	337	343	PF00179	0.643
MOD_SUMO_rev_2	510	515	PF00179	0.475
TRG_AP2beta_CARGO_1	634	644	PF09066	0.518
TRG_DiLeu_BaLyEn_6	603	608	PF01217	0.518
TRG_DiLeu_BaLyEn_6	757	762	PF01217	0.518
TRG_ENDOCYTIC_2	391	394	PF00928	0.521
TRG_ENDOCYTIC_2	406	409	PF00928	0.476
TRG_ENDOCYTIC_2	619	622	PF00928	0.521
TRG_ENDOCYTIC_2	625	628	PF00928	0.517
TRG_ENDOCYTIC_2	671	674	PF00928	0.486
TRG_ENDOCYTIC_2	709	712	PF00928	0.510
TRG_ENDOCYTIC_2	731	734	PF00928	0.493
TRG_ER_diArg_1	320	323	PF00400	0.660
TRG_ER_diArg_1	355	357	PF00400	0.502
TRG_ER_diArg_1	394	396	PF00400	0.546
TRG_ER_diArg_1	443	445	PF00400	0.475
TRG_ER_diArg_1	477	479	PF00400	0.522
TRG_ER_diArg_1	791	794	PF00400	0.447
TRG_NES_CRM1_1	653	668	PF08389	0.518
TRG_NLS_MonoCore_2	55	60	PF00514	0.632
TRG_NLS_MonoExtN_4	56	61	PF00514	0.629
TRG_NLS_MonoExtN_4	97	104	PF00514	0.692
TRG_Pf-PMV_PEXEL_1	161	166	PF00026	0.700
TRG_Pf-PMV_PEXEL_1	396	400	PF00026	0.318
TRG_Pf-PMV_PEXEL_1	760	764	PF00026	0.318

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PDX5	Leptomonas seymouri	59%	100%
A0A3Q8IDL7	Leishmania donovani	23%	100%
A0A3S5H6X0	Leishmania donovani	89%	99%
A4H8G3	Leishmania braziliensis	71%	100%
A4HWT7	Leishmania infantum	90%	99%
A4HYN9	Leishmania infantum	23%	100%
E9AQJ5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	84%	99%
E9AUL7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	24%	100%
Q4QCW7	Leishmania major	24%	100%