Alpha/beta knot methyltransferase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

EMG1/NEP1 methyltransferase, putative

Species:

Leishmania major

UniProt:

Q4QEM7_LEIMA

TriTrypDb:

LmjF.16.1390 , LMJLV39_160020500 , LMJSD75_160020100

Length:

287

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005730	nucleolus	5	2
GO:0030684	preribosome	3	2
GO:0032040	small-subunit processome	4	2
GO:0032991	protein-containing complex	1	2
GO:0043226	organelle	2	2
GO:0043228	non-membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043232	intracellular non-membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	2
GO:1990904	ribonucleoprotein complex	2	2

Expansion

Sequence features

Q4QEM7

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QEM7

Function

Biological processes
Term	Name	Level	Count
GO:0000154	rRNA modification	6	12
GO:0001510	RNA methylation	4	12
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006364	rRNA processing	8	12
GO:0006396	RNA processing	6	12
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008152	metabolic process	1	12
GO:0009451	RNA modification	5	12
GO:0009987	cellular process	1	12
GO:0016070	RNA metabolic process	5	12
GO:0016072	rRNA metabolic process	7	12
GO:0031167	rRNA methylation	5	12
GO:0032259	methylation	2	12
GO:0034470	ncRNA processing	7	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0034660	ncRNA metabolic process	6	12
GO:0043170	macromolecule metabolic process	3	12
GO:0043412	macromolecule modification	4	12
GO:0043414	macromolecule methylation	3	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0044260	obsolete cellular macromolecule metabolic process	3	12
GO:0046483	heterocycle metabolic process	3	12
GO:0070475	rRNA base methylation	6	12
GO:0071704	organic substance metabolic process	2	12
GO:0090304	nucleic acid metabolic process	4	12
GO:1901360	organic cyclic compound metabolic process	3	12

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	12
GO:0003723	RNA binding	4	12
GO:0003824	catalytic activity	1	12
GO:0005488	binding	1	12
GO:0008168	methyltransferase activity	4	12
GO:0008173	RNA methyltransferase activity	4	12
GO:0008649	rRNA methyltransferase activity	5	12
GO:0008757	S-adenosylmethionine-dependent methyltransferase activity	5	12
GO:0016740	transferase activity	2	12
GO:0016741	transferase activity, transferring one-carbon groups	3	12
GO:0019843	rRNA binding	5	12
GO:0070037	rRNA (pseudouridine) methyltransferase activity	6	12
GO:0097159	organic cyclic compound binding	2	12
GO:0140098	catalytic activity, acting on RNA	3	12
GO:0140102	catalytic activity, acting on a rRNA	4	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	12
GO:1901363	heterocyclic compound binding	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	176	180	PF00656	0.210
CLV_NRD_NRD_1	184	186	PF00675	0.405
CLV_NRD_NRD_1	192	194	PF00675	0.358
CLV_NRD_NRD_1	218	220	PF00675	0.421
CLV_NRD_NRD_1	224	226	PF00675	0.322
CLV_PCSK_KEX2_1	191	193	PF00082	0.383
CLV_PCSK_KEX2_1	21	23	PF00082	0.370
CLV_PCSK_KEX2_1	224	226	PF00082	0.417
CLV_PCSK_PC1ET2_1	21	23	PF00082	0.464
CLV_PCSK_SKI1_1	192	196	PF00082	0.407
DEG_Nend_UBRbox_2	1	3	PF02207	0.566
DOC_CYCLIN_yCln2_LP_2	161	167	PF00134	0.363
DOC_MAPK_gen_1	102	111	PF00069	0.283
DOC_MAPK_gen_1	21	27	PF00069	0.358
DOC_MAPK_gen_1	90	99	PF00069	0.283
DOC_MAPK_HePTP_8	87	99	PF00069	0.296
DOC_MAPK_MEF2A_6	110	119	PF00069	0.113
DOC_MAPK_MEF2A_6	81	89	PF00069	0.289
DOC_MAPK_MEF2A_6	90	99	PF00069	0.273
DOC_PP2B_LxvP_1	103	106	PF13499	0.296
DOC_USP7_MATH_1	4	8	PF00917	0.621
DOC_USP7_MATH_2	249	255	PF00917	0.417
DOC_USP7_UBL2_3	17	21	PF12436	0.529
DOC_USP7_UBL2_3	216	220	PF12436	0.404
DOC_WW_Pin1_4	74	79	PF00397	0.289
LIG_14-3-3_CanoR_1	126	134	PF00244	0.352
LIG_14-3-3_CanoR_1	185	190	PF00244	0.398
LIG_14-3-3_CanoR_1	192	202	PF00244	0.456
LIG_14-3-3_CanoR_1	260	268	PF00244	0.310
LIG_14-3-3_CanoR_1	47	52	PF00244	0.396
LIG_AP2alpha_2	163	165	PF02296	0.377
LIG_BRCT_BRCA1_1	117	121	PF00533	0.417
LIG_FHA_1	127	133	PF00498	0.367
LIG_FHA_1	141	147	PF00498	0.202
LIG_FHA_2	199	205	PF00498	0.417
LIG_Integrin_RGD_1	239	241	PF01839	0.417
LIG_LIR_Apic_2	243	249	PF02991	0.417
LIG_LIR_Gen_1	128	138	PF02991	0.381
LIG_LIR_Gen_1	38	46	PF02991	0.318
LIG_LIR_Nem_3	128	134	PF02991	0.381
LIG_LIR_Nem_3	18	23	PF02991	0.445
LIG_LIR_Nem_3	38	44	PF02991	0.318
LIG_PDZ_Class_3	282	287	PF00595	0.640
LIG_Pex14_2	275	279	PF04695	0.388
LIG_SH2_CRK	246	250	PF00017	0.389
LIG_SH2_NCK_1	246	250	PF00017	0.417
LIG_SH2_STAP1	173	177	PF00017	0.388
LIG_SH2_STAT5	131	134	PF00017	0.381
LIG_SH3_3	135	141	PF00018	0.296
LIG_SUMO_SIM_par_1	22	28	PF11976	0.312
LIG_TRAF2_1	13	16	PF00917	0.464
LIG_TRAF2_1	201	204	PF00917	0.443
LIG_UBA3_1	115	124	PF00899	0.400
MOD_CDK_SPxxK_3	74	81	PF00069	0.289
MOD_CK1_1	196	202	PF00069	0.336
MOD_CK2_1	10	16	PF00069	0.477
MOD_CK2_1	198	204	PF00069	0.417
MOD_GlcNHglycan	12	15	PF01048	0.483
MOD_GlcNHglycan	198	201	PF01048	0.359
MOD_GlcNHglycan	262	265	PF01048	0.296
MOD_GlcNHglycan	6	9	PF01048	0.506
MOD_GSK3_1	126	133	PF00069	0.381
MOD_GSK3_1	163	170	PF00069	0.405
MOD_GSK3_1	192	199	PF00069	0.355
MOD_NEK2_1	115	120	PF00069	0.312
MOD_NEK2_1	167	172	PF00069	0.359
MOD_NEK2_1	194	199	PF00069	0.411
MOD_NEK2_1	202	207	PF00069	0.417
MOD_NEK2_1	88	93	PF00069	0.288
MOD_PKA_1	185	191	PF00069	0.459
MOD_PKA_1	192	198	PF00069	0.364
MOD_PKA_2	125	131	PF00069	0.381
MOD_PKA_2	192	198	PF00069	0.417
MOD_PKB_1	191	199	PF00069	0.396
MOD_Plk_1	240	246	PF00069	0.393
MOD_Plk_1	250	256	PF00069	0.301
MOD_Plk_2-3	208	214	PF00069	0.443
MOD_Plk_4	115	121	PF00069	0.356
MOD_Plk_4	130	136	PF00069	0.204
MOD_Plk_4	267	273	PF00069	0.398
MOD_ProDKin_1	74	80	PF00069	0.289
MOD_SUMO_rev_2	179	188	PF00179	0.365
TRG_ENDOCYTIC_2	131	134	PF00928	0.381
TRG_ER_diArg_1	101	104	PF00400	0.289
TRG_ER_diArg_1	191	193	PF00400	0.405

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PB82	Leptomonas seymouri	92%	100%
A0A0S4IWL3	Bodo saltans	57%	100%
A0A1X0NZ96	Trypanosomatidae	60%	96%
A0A3Q8ICM7	Leishmania donovani	97%	100%
A0A3R7KPC0	Trypanosoma rangeli	64%	100%
A4H8R8	Leishmania braziliensis	93%	100%
A4HX36	Leishmania infantum	98%	100%
C9ZVV5	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	63%	100%
E9AQV1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	97%	100%
O35130	Mus musculus	38%	100%
Q06287	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	34%	100%
Q10107	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	35%	80%
Q92979	Homo sapiens	37%	100%
Q96UP2	Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65)	35%	100%
Q9W4J5	Drosophila melanogaster	33%	100%
Q9XX15	Caenorhabditis elegans	32%	100%
V5B2L3	Trypanosoma cruzi	62%	100%