Putative eukaryotic translation initiation factor 4 gamma

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

eukaryotic translation initiation factor 4 gamma 3

Species:

Leishmania major

UniProt:

Q4QEK5_LEIMA

TriTrypDb:

LmjF.16.1600 , LMJLV39_160022900 , LMJSD75_160022600

Length:

635

Annotations

LeishMANIAdb interaction annotations

by homology
Contact email: opmn@cpqam.biocruz.br
Publication title: Translation initiation in Leishmania major: characterisation of multiple eIF4F subunit homologues
Publication 1st author(s): Dhalia
Publication Identifier(s): 15694484
Host organism: -1
Interaction detection method(s): pull down
Interaction type: physical association
Identification method participant A: autoradiography
Identification method participant B: autoradiography
ID(s) interactor A: P60842
ID(s) interactor B: Q4QEK5
Taxid interactor A: Homo sapiens
Taxid interactor B: Leishmania major
Biological role(s) interactor A: unspecified role
Biological role(s) interactor B: unspecified role
Experimental role(s) interactor A: bait

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 12
NetGPI	no	yes: 0, no: 12

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	2
GO:0010494	cytoplasmic stress granule	5	2
GO:0016281	eukaryotic translation initiation factor 4F complex	2	2
GO:0032991	protein-containing complex	1	2
GO:0035770	ribonucleoprotein granule	3	2
GO:0036464	cytoplasmic ribonucleoprotein granule	4	2
GO:0043226	organelle	2	2
GO:0043228	non-membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043232	intracellular non-membrane-bounded organelle	4	2
GO:0099080	supramolecular complex	2	2
GO:0110165	cellular anatomical entity	1	3
GO:0016020	membrane	2	1

Expansion

Sequence features

Q4QEK5

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QEK5

Function

Could not find GO biological_process term for this entry.

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	13
GO:0003723	RNA binding	4	13
GO:0003729	mRNA binding	5	2
GO:0003743	translation initiation factor activity	4	13
GO:0005488	binding	1	13
GO:0008135	translation factor activity, RNA binding	3	13
GO:0045182	translation regulator activity	1	13
GO:0090079	translation regulator activity, nucleic acid binding	2	13
GO:0097159	organic cyclic compound binding	2	13
GO:1901363	heterocyclic compound binding	2	13

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	329	333	PF00656	0.664
CLV_C14_Caspase3-7	456	460	PF00656	0.464
CLV_NRD_NRD_1	182	184	PF00675	0.240
CLV_NRD_NRD_1	281	283	PF00675	0.247
CLV_NRD_NRD_1	32	34	PF00675	0.477
CLV_NRD_NRD_1	456	458	PF00675	0.502
CLV_PCSK_FUR_1	182	186	PF00082	0.285
CLV_PCSK_FUR_1	30	34	PF00082	0.550
CLV_PCSK_KEX2_1	182	184	PF00082	0.239
CLV_PCSK_KEX2_1	283	285	PF00082	0.443
CLV_PCSK_KEX2_1	30	32	PF00082	0.449
CLV_PCSK_PC1ET2_1	184	186	PF00082	0.256
CLV_PCSK_PC1ET2_1	283	285	PF00082	0.592
CLV_PCSK_SKI1_1	103	107	PF00082	0.313
CLV_PCSK_SKI1_1	291	295	PF00082	0.679
CLV_PCSK_SKI1_1	306	310	PF00082	0.633
CLV_PCSK_SKI1_1	32	36	PF00082	0.566
CLV_PCSK_SKI1_1	462	466	PF00082	0.462
CLV_PCSK_SKI1_1	81	85	PF00082	0.342
CLV_Separin_Metazoa	27	31	PF03568	0.503
DEG_APCC_DBOX_1	567	575	PF00400	0.536
DEG_Nend_UBRbox_3	1	3	PF02207	0.635
DEG_SPOP_SBC_1	368	372	PF00917	0.642
DEG_SPOP_SBC_1	452	456	PF00917	0.441
DOC_CYCLIN_RxL_1	81	92	PF00134	0.462
DOC_MAPK_gen_1	182	190	PF00069	0.434
DOC_MAPK_gen_1	270	278	PF00069	0.454
DOC_MAPK_MEF2A_6	388	397	PF00069	0.635
DOC_PP1_RVXF_1	268	274	PF00149	0.434
DOC_PP2B_LxvP_1	16	19	PF13499	0.500
DOC_SPAK_OSR1_1	272	276	PF12202	0.485
DOC_USP7_MATH_1	109	113	PF00917	0.478
DOC_USP7_MATH_1	324	328	PF00917	0.718
DOC_USP7_MATH_1	344	348	PF00917	0.747
DOC_USP7_MATH_1	368	372	PF00917	0.545
DOC_USP7_MATH_1	383	387	PF00917	0.784
DOC_USP7_MATH_1	486	490	PF00917	0.328
DOC_USP7_MATH_1	541	545	PF00917	0.409
DOC_USP7_MATH_1	557	561	PF00917	0.382
DOC_USP7_UBL2_3	160	164	PF12436	0.481
DOC_USP7_UBL2_3	243	247	PF12436	0.401
DOC_USP7_UBL2_3	81	85	PF12436	0.525
DOC_WW_Pin1_4	389	394	PF00397	0.697
DOC_WW_Pin1_4	537	542	PF00397	0.457
LIG_14-3-3_CanoR_1	138	147	PF00244	0.506
LIG_14-3-3_CanoR_1	214	222	PF00244	0.488
LIG_14-3-3_CanoR_1	31	40	PF00244	0.488
LIG_14-3-3_CanoR_1	355	362	PF00244	0.711
LIG_14-3-3_CanoR_1	403	408	PF00244	0.356
LIG_14-3-3_CanoR_1	505	510	PF00244	0.380
LIG_14-3-3_CanoR_1	532	536	PF00244	0.359
LIG_14-3-3_CanoR_1	593	599	PF00244	0.367
LIG_Actin_WH2_2	115	133	PF00022	0.579
LIG_APCC_ABBAyCdc20_2	520	526	PF00400	0.475
LIG_BH_BH3_1	226	242	PF00452	0.488
LIG_BRCT_BRCA1_1	202	206	PF00533	0.531
LIG_Clathr_ClatBox_1	397	401	PF01394	0.532
LIG_DLG_GKlike_1	505	512	PF00625	0.500
LIG_Dynein_DLC8_1	31	37	PF01221	0.529
LIG_EH1_1	272	280	PF00400	0.454
LIG_FHA_1	35	41	PF00498	0.507
LIG_FHA_1	364	370	PF00498	0.659
LIG_FHA_1	414	420	PF00498	0.584
LIG_FHA_1	82	88	PF00498	0.462
LIG_FHA_2	205	211	PF00498	0.518
LIG_FHA_2	234	240	PF00498	0.492
LIG_FHA_2	252	258	PF00498	0.488
LIG_FHA_2	454	460	PF00498	0.399
LIG_FHA_2	511	517	PF00498	0.470
LIG_FHA_2	52	58	PF00498	0.435
LIG_FHA_2	559	565	PF00498	0.585
LIG_GBD_Chelix_1	487	495	PF00786	0.409
LIG_Integrin_isoDGR_2	353	355	PF01839	0.676
LIG_LIR_Gen_1	203	213	PF02991	0.542
LIG_LIR_Gen_1	250	259	PF02991	0.511
LIG_LIR_Gen_1	463	473	PF02991	0.355
LIG_LIR_Gen_1	504	512	PF02991	0.367
LIG_LIR_Gen_1	534	541	PF02991	0.426
LIG_LIR_Gen_1	551	559	PF02991	0.308
LIG_LIR_Gen_1	610	617	PF02991	0.463
LIG_LIR_LC3C_4	466	470	PF02991	0.473
LIG_LIR_Nem_3	112	118	PF02991	0.443
LIG_LIR_Nem_3	154	158	PF02991	0.566
LIG_LIR_Nem_3	17	23	PF02991	0.433
LIG_LIR_Nem_3	250	256	PF02991	0.481
LIG_LIR_Nem_3	463	468	PF02991	0.329
LIG_LIR_Nem_3	477	483	PF02991	0.275
LIG_LIR_Nem_3	534	538	PF02991	0.387
LIG_LIR_Nem_3	551	556	PF02991	0.299
LIG_LIR_Nem_3	610	615	PF02991	0.453
LIG_NRBOX	229	235	PF00104	0.509
LIG_NRBOX	586	592	PF00104	0.474
LIG_PCNA_yPIPBox_3	415	424	PF02747	0.550
LIG_PCNA_yPIPBox_3	584	593	PF02747	0.449
LIG_Pex14_1	423	427	PF04695	0.361
LIG_Pex14_2	502	506	PF04695	0.325
LIG_PTB_Apo_2	96	103	PF02174	0.531
LIG_SH2_CRK	115	119	PF00017	0.433
LIG_SH2_NCK_1	307	311	PF00017	0.605
LIG_SH2_SRC	15	18	PF00017	0.506
LIG_SH2_STAP1	125	129	PF00017	0.528
LIG_SH2_STAT5	15	18	PF00017	0.650
LIG_SH2_STAT5	195	198	PF00017	0.509
LIG_SH2_STAT5	252	255	PF00017	0.454
LIG_SH2_STAT5	524	527	PF00017	0.409
LIG_SH3_1	15	21	PF00018	0.513
LIG_SH3_3	15	21	PF00018	0.514
LIG_SH3_3	263	269	PF00018	0.486
LIG_SH3_3	390	396	PF00018	0.649
LIG_SH3_3	553	559	PF00018	0.463
LIG_SUMO_SIM_anti_2	223	231	PF11976	0.494
LIG_SUMO_SIM_anti_2	570	576	PF11976	0.528
LIG_TRAF2_1	169	172	PF00917	0.491
LIG_TRAF2_1	515	518	PF00917	0.628
LIG_TRAF2_1	54	57	PF00917	0.494
LIG_TRAF2_1	561	564	PF00917	0.634
LIG_TYR_ITIM	533	538	PF00017	0.417
LIG_UBA3_1	275	283	PF00899	0.468
LIG_UBA3_1	78	85	PF00899	0.455
LIG_WRC_WIRS_1	549	554	PF05994	0.492
LIG_WW_3	267	271	PF00397	0.485
MOD_CK1_1	216	222	PF00069	0.577
MOD_CK1_1	372	378	PF00069	0.678
MOD_CK1_1	451	457	PF00069	0.571
MOD_CK2_1	160	166	PF00069	0.460
MOD_CK2_1	21	27	PF00069	0.468
MOD_CK2_1	251	257	PF00069	0.488
MOD_CK2_1	433	439	PF00069	0.545
MOD_CK2_1	486	492	PF00069	0.342
MOD_CK2_1	51	57	PF00069	0.431
MOD_CK2_1	510	516	PF00069	0.452
MOD_CK2_1	557	563	PF00069	0.564
MOD_GlcNHglycan	321	324	PF01048	0.664
MOD_GlcNHglycan	346	349	PF01048	0.699
MOD_GlcNHglycan	358	361	PF01048	0.699
MOD_GlcNHglycan	371	374	PF01048	0.679
MOD_GlcNHglycan	450	453	PF01048	0.496
MOD_GlcNHglycan	484	487	PF01048	0.315
MOD_GlcNHglycan	488	491	PF01048	0.305
MOD_GlcNHglycan	542	546	PF01048	0.567
MOD_GSK3_1	156	163	PF00069	0.540
MOD_GSK3_1	200	207	PF00069	0.561
MOD_GSK3_1	209	216	PF00069	0.540
MOD_GSK3_1	333	340	PF00069	0.728
MOD_GSK3_1	363	370	PF00069	0.756
MOD_GSK3_1	373	380	PF00069	0.581
MOD_GSK3_1	448	455	PF00069	0.449
MOD_GSK3_1	48	55	PF00069	0.477
MOD_GSK3_1	482	489	PF00069	0.450
MOD_GSK3_1	512	519	PF00069	0.484
MOD_GSK3_1	537	544	PF00069	0.533
MOD_N-GLC_2	428	430	PF02516	0.520
MOD_NEK2_1	200	205	PF00069	0.498
MOD_NEK2_1	209	214	PF00069	0.522
MOD_NEK2_1	233	238	PF00069	0.459
MOD_NEK2_1	34	39	PF00069	0.522
MOD_NEK2_1	369	374	PF00069	0.643
MOD_NEK2_1	510	515	PF00069	0.417
MOD_NEK2_1	531	536	PF00069	0.421
MOD_NEK2_1	63	68	PF00069	0.543
MOD_NEK2_2	383	388	PF00069	0.569
MOD_PIKK_1	138	144	PF00454	0.584
MOD_PIKK_1	32	38	PF00454	0.509
MOD_PKA_1	183	189	PF00069	0.531
MOD_PKA_1	32	38	PF00069	0.491
MOD_PKA_2	213	219	PF00069	0.488
MOD_PKA_2	32	38	PF00069	0.513
MOD_PKA_2	354	360	PF00069	0.737
MOD_PKA_2	510	516	PF00069	0.451
MOD_PKA_2	531	537	PF00069	0.475
MOD_PKB_1	30	38	PF00069	0.509
MOD_Plk_1	209	215	PF00069	0.550
MOD_Plk_1	541	547	PF00069	0.417
MOD_Plk_2-3	166	172	PF00069	0.468
MOD_Plk_4	201	207	PF00069	0.462
MOD_Plk_4	403	409	PF00069	0.451
MOD_Plk_4	505	511	PF00069	0.449
MOD_Plk_4	545	551	PF00069	0.366
MOD_Plk_4	594	600	PF00069	0.433
MOD_ProDKin_1	389	395	PF00069	0.691
MOD_ProDKin_1	537	543	PF00069	0.459
MOD_SUMO_for_1	398	401	PF00179	0.443
MOD_SUMO_rev_2	121	130	PF00179	0.435
MOD_SUMO_rev_2	161	169	PF00179	0.488
MOD_SUMO_rev_2	300	308	PF00179	0.664
MOD_SUMO_rev_2	72	77	PF00179	0.563
TRG_DiLeu_BaEn_1	225	230	PF01217	0.488
TRG_DiLeu_BaEn_2	462	468	PF01217	0.326
TRG_DiLeu_BaEn_3	72	78	PF01217	0.462
TRG_DiLeu_BaLyEn_6	58	63	PF01217	0.411
TRG_ENDOCYTIC_2	115	118	PF00928	0.435
TRG_ENDOCYTIC_2	20	23	PF00928	0.436
TRG_ENDOCYTIC_2	535	538	PF00928	0.343
TRG_ER_diArg_1	181	183	PF00400	0.450
TRG_ER_diArg_1	269	272	PF00400	0.463
TRG_ER_diArg_1	281	284	PF00400	0.436
TRG_ER_diArg_1	29	32	PF00400	0.502
TRG_NES_CRM1_1	547	562	PF08389	0.591
TRG_NES_CRM1_1	607	619	PF08389	0.518
TRG_NLS_MonoExtN_4	182	187	PF00514	0.456
TRG_Pf-PMV_PEXEL_1	104	108	PF00026	0.233
TRG_Pf-PMV_PEXEL_1	156	161	PF00026	0.365
TRG_Pf-PMV_PEXEL_1	189	193	PF00026	0.355
TRG_Pf-PMV_PEXEL_1	32	36	PF00026	0.515

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PDA5	Leptomonas seymouri	67%	100%
A0A0S4IUF0	Bodo saltans	34%	98%
A0A0S4KJH6	Bodo saltans	25%	100%
A0A1X0NZC7	Trypanosomatidae	41%	100%
A0A3Q8IAL0	Leishmania donovani	96%	100%
A0A422NEE1	Trypanosoma rangeli	42%	100%
A4H8T8	Leishmania braziliensis	92%	100%
A4HX58	Leishmania infantum	97%	100%
C9ZVT3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	40%	100%
E9AQX3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	97%	100%
V5DJ79	Trypanosoma cruzi	43%	100%