Separase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Separase

Gene product:

eukaryotic translation initiation factor 3 subunit a

Species:

Leishmania major

UniProt:

Q4QEJ8_LEIMA

TriTrypDb:

LmjF.17.0010 , LMJLV39_170005000 , LMJSD75_170005100

Length:

774

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 2
Forrest at al. (procyclic)	no	yes: 2
Silverman et al.	yes	yes: 2
Pissara et al.	yes	yes: 10

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	yes	yes: 4

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 4

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	2
GO:0005852	eukaryotic translation initiation factor 3 complex	2	12
GO:0032991	protein-containing complex	1	12
GO:0043614	multi-eIF complex	3	2
GO:0071540	eukaryotic translation initiation factor 3 complex, eIF3e	3	2
GO:0071541	eukaryotic translation initiation factor 3 complex, eIF3m	3	2
GO:0110165	cellular anatomical entity	1	2
GO:1990904	ribonucleoprotein complex	2	2

Expansion

Sequence features

Q4QEJ8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QEJ8

Function

Biological processes
Term	Name	Level	Count
GO:0001732	formation of cytoplasmic translation initiation complex	7	2
GO:0002183	cytoplasmic translational initiation	4	2
GO:0002188	translation reinitiation	5	2
GO:0006413	translational initiation	3	2
GO:0008152	metabolic process	1	2
GO:0009987	cellular process	1	2
GO:0016043	cellular component organization	3	2
GO:0022607	cellular component assembly	4	2
GO:0022618	ribonucleoprotein complex assembly	6	2
GO:0043933	protein-containing complex organization	4	2
GO:0044237	cellular metabolic process	2	2
GO:0065003	protein-containing complex assembly	5	2
GO:0071826	ribonucleoprotein complex subunit organization	5	2
GO:0071840	cellular component organization or biogenesis	2	2

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	11
GO:0003723	RNA binding	4	2
GO:0003729	mRNA binding	5	2
GO:0003743	translation initiation factor activity	4	11
GO:0005488	binding	1	11
GO:0008135	translation factor activity, RNA binding	3	11
GO:0045182	translation regulator activity	1	11
GO:0090079	translation regulator activity, nucleic acid binding	2	11
GO:0097159	organic cyclic compound binding	2	11
GO:1901363	heterocyclic compound binding	2	11

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	29	33	PF00656	0.584
CLV_C14_Caspase3-7	490	494	PF00656	0.673
CLV_NRD_NRD_1	151	153	PF00675	0.638
CLV_NRD_NRD_1	590	592	PF00675	0.730
CLV_NRD_NRD_1	606	608	PF00675	0.547
CLV_NRD_NRD_1	626	628	PF00675	0.365
CLV_NRD_NRD_1	663	665	PF00675	0.660
CLV_NRD_NRD_1	669	671	PF00675	0.677
CLV_NRD_NRD_1	690	692	PF00675	0.635
CLV_NRD_NRD_1	718	720	PF00675	0.611
CLV_NRD_NRD_1	723	725	PF00675	0.592
CLV_NRD_NRD_1	74	76	PF00675	0.608
CLV_NRD_NRD_1	745	747	PF00675	0.783
CLV_NRD_NRD_1	769	771	PF00675	0.755
CLV_PCSK_KEX2_1	140	142	PF00082	0.660
CLV_PCSK_KEX2_1	151	153	PF00082	0.450
CLV_PCSK_KEX2_1	272	274	PF00082	0.591
CLV_PCSK_KEX2_1	319	321	PF00082	0.683
CLV_PCSK_KEX2_1	323	325	PF00082	0.369
CLV_PCSK_KEX2_1	537	539	PF00082	0.733
CLV_PCSK_KEX2_1	582	584	PF00082	0.667
CLV_PCSK_KEX2_1	590	592	PF00082	0.734
CLV_PCSK_KEX2_1	662	664	PF00082	0.635
CLV_PCSK_KEX2_1	690	692	PF00082	0.618
CLV_PCSK_KEX2_1	718	720	PF00082	0.633
CLV_PCSK_KEX2_1	723	725	PF00082	0.580
CLV_PCSK_KEX2_1	74	76	PF00082	0.626
CLV_PCSK_KEX2_1	747	749	PF00082	0.700
CLV_PCSK_KEX2_1	756	758	PF00082	0.594
CLV_PCSK_KEX2_1	769	771	PF00082	0.634
CLV_PCSK_PC1ET2_1	140	142	PF00082	0.660
CLV_PCSK_PC1ET2_1	272	274	PF00082	0.591
CLV_PCSK_PC1ET2_1	319	321	PF00082	0.683
CLV_PCSK_PC1ET2_1	323	325	PF00082	0.369
CLV_PCSK_PC1ET2_1	537	539	PF00082	0.820
CLV_PCSK_PC1ET2_1	582	584	PF00082	0.681
CLV_PCSK_PC1ET2_1	662	664	PF00082	0.643
CLV_PCSK_PC1ET2_1	690	692	PF00082	0.637
CLV_PCSK_PC1ET2_1	747	749	PF00082	0.700
CLV_PCSK_PC1ET2_1	756	758	PF00082	0.594
CLV_PCSK_PC7_1	719	725	PF00082	0.709
CLV_PCSK_PC7_1	743	749	PF00082	0.699
CLV_PCSK_SKI1_1	167	171	PF00082	0.578
CLV_PCSK_SKI1_1	178	182	PF00082	0.507
CLV_PCSK_SKI1_1	231	235	PF00082	0.570
CLV_PCSK_SKI1_1	3	7	PF00082	0.755
CLV_PCSK_SKI1_1	330	334	PF00082	0.488
CLV_PCSK_SKI1_1	409	413	PF00082	0.613
CLV_PCSK_SKI1_1	430	434	PF00082	0.526
CLV_PCSK_SKI1_1	623	627	PF00082	0.547
CLV_PCSK_SKI1_1	671	675	PF00082	0.704
CLV_PCSK_SKI1_1	728	732	PF00082	0.632
CLV_Separin_Metazoa	624	628	PF03568	0.658
CLV_Separin_Metazoa	71	75	PF03568	0.632
DEG_APCC_DBOX_1	329	337	PF00400	0.478
DEG_APCC_DBOX_1	433	441	PF00400	0.593
DEG_Nend_UBRbox_2	1	3	PF02207	0.815
DEG_SPOP_SBC_1	532	536	PF00917	0.785
DEG_SPOP_SBC_1	552	556	PF00917	0.386
DOC_CKS1_1	42	47	PF01111	0.666
DOC_MAPK_DCC_7	39	48	PF00069	0.684
DOC_MAPK_gen_1	109	117	PF00069	0.643
DOC_MAPK_gen_1	277	287	PF00069	0.665
DOC_MAPK_gen_1	537	543	PF00069	0.722
DOC_MAPK_MEF2A_6	92	101	PF00069	0.547
DOC_MAPK_RevD_3	127	141	PF00069	0.657
DOC_PP1_RVXF_1	165	172	PF00149	0.591
DOC_PP2B_LxvP_1	686	689	PF13499	0.568
DOC_PP4_FxxP_1	187	190	PF00568	0.613
DOC_PP4_FxxP_1	362	365	PF00568	0.693
DOC_USP7_MATH_1	11	15	PF00917	0.661
DOC_USP7_MATH_1	33	37	PF00917	0.572
DOC_USP7_MATH_1	4	8	PF00917	0.749
DOC_USP7_MATH_1	416	420	PF00917	0.704
DOC_USP7_MATH_1	514	518	PF00917	0.781
DOC_USP7_MATH_1	519	523	PF00917	0.797
DOC_USP7_MATH_1	532	536	PF00917	0.813
DOC_USP7_MATH_1	575	579	PF00917	0.744
DOC_USP7_UBL2_3	319	323	PF12436	0.681
DOC_USP7_UBL2_3	577	581	PF12436	0.720
DOC_USP7_UBL2_3	628	632	PF12436	0.600
DOC_WW_Pin1_4	361	366	PF00397	0.699
DOC_WW_Pin1_4	383	388	PF00397	0.652
DOC_WW_Pin1_4	41	46	PF00397	0.685
LIG_14-3-3_CanoR_1	10	16	PF00244	0.675
LIG_14-3-3_CanoR_1	202	209	PF00244	0.646
LIG_14-3-3_CanoR_1	485	490	PF00244	0.665
LIG_14-3-3_CanoR_1	524	531	PF00244	0.843
LIG_14-3-3_CanoR_1	551	560	PF00244	0.653
LIG_Actin_WH2_2	65	83	PF00022	0.644
LIG_AP2alpha_2	346	348	PF02296	0.674
LIG_BRCT_BRCA1_1	295	299	PF00533	0.573
LIG_FHA_1	236	242	PF00498	0.536
LIG_FHA_1	299	305	PF00498	0.610
LIG_FHA_1	368	374	PF00498	0.639
LIG_FHA_1	376	382	PF00498	0.633
LIG_FHA_1	484	490	PF00498	0.593
LIG_FHA_1	653	659	PF00498	0.550
LIG_FHA_2	118	124	PF00498	0.664
LIG_FHA_2	292	298	PF00498	0.562
LIG_FHA_2	458	464	PF00498	0.649
LIG_FHA_2	700	706	PF00498	0.717
LIG_IRF3_LxIS_1	190	197	PF10401	0.623
LIG_LIR_Apic_2	186	190	PF02991	0.577
LIG_LIR_Apic_2	359	365	PF02991	0.691
LIG_LIR_Gen_1	282	292	PF02991	0.616
LIG_LIR_Gen_1	398	407	PF02991	0.628
LIG_LIR_Gen_1	443	454	PF02991	0.666
LIG_LIR_Gen_1	501	510	PF02991	0.625
LIG_LIR_Gen_1	522	533	PF02991	0.813
LIG_LIR_Nem_3	282	287	PF02991	0.669
LIG_LIR_Nem_3	346	351	PF02991	0.729
LIG_LIR_Nem_3	398	404	PF02991	0.626
LIG_LIR_Nem_3	443	449	PF02991	0.663
LIG_LIR_Nem_3	501	506	PF02991	0.633
LIG_LIR_Nem_3	522	528	PF02991	0.811
LIG_NRBOX	247	253	PF00104	0.582
LIG_NRBOX	653	659	PF00104	0.584
LIG_PCNA_yPIPBox_3	167	181	PF02747	0.594
LIG_PCNA_yPIPBox_3	350	362	PF02747	0.653
LIG_PCNA_yPIPBox_3	91	102	PF02747	0.638
LIG_Pex14_2	741	745	PF04695	0.727
LIG_RPA_C_Fungi	548	560	PF08784	0.696
LIG_SH2_GRB2like	464	467	PF00017	0.620
LIG_SH2_GRB2like	502	505	PF00017	0.732
LIG_SH2_PTP2	446	449	PF00017	0.663
LIG_SH2_SRC	446	449	PF00017	0.653
LIG_SH2_STAP1	679	683	PF00017	0.685
LIG_SH2_STAT3	558	561	PF00017	0.699
LIG_SH2_STAT3	694	697	PF00017	0.753
LIG_SH2_STAT5	310	313	PF00017	0.518
LIG_SH2_STAT5	446	449	PF00017	0.595
LIG_SH2_STAT5	464	467	PF00017	0.420
LIG_SH2_STAT5	502	505	PF00017	0.732
LIG_SH2_STAT5	605	608	PF00017	0.693
LIG_SH2_STAT5	713	716	PF00017	0.711
LIG_SUMO_SIM_anti_2	282	289	PF11976	0.669
LIG_SUMO_SIM_anti_2	49	56	PF11976	0.595
LIG_SUMO_SIM_par_1	192	198	PF11976	0.590
LIG_SUMO_SIM_par_1	469	477	PF11976	0.639
LIG_TRAF2_1	223	226	PF00917	0.658
LIG_UBA3_1	372	379	PF00899	0.635
MOD_CK1_1	13	19	PF00069	0.678
MOD_CK1_1	198	204	PF00069	0.584
MOD_CK1_1	239	245	PF00069	0.538
MOD_CK1_1	364	370	PF00069	0.667
MOD_CK1_1	402	408	PF00069	0.664
MOD_CK1_1	517	523	PF00069	0.810
MOD_CK1_1	526	532	PF00069	0.845
MOD_CK2_1	457	463	PF00069	0.661
MOD_CK2_1	533	539	PF00069	0.804
MOD_CK2_1	564	570	PF00069	0.645
MOD_CK2_1	699	705	PF00069	0.715
MOD_GlcNHglycan	197	200	PF01048	0.532
MOD_GlcNHglycan	238	241	PF01048	0.560
MOD_GlcNHglycan	288	291	PF01048	0.562
MOD_GlcNHglycan	440	443	PF01048	0.625
MOD_GlcNHglycan	521	524	PF01048	0.831
MOD_GlcNHglycan	645	648	PF01048	0.684
MOD_GlcNHglycan	77	80	PF01048	0.637
MOD_GSK3_1	194	201	PF00069	0.545
MOD_GSK3_1	235	242	PF00069	0.576
MOD_GSK3_1	310	317	PF00069	0.548
MOD_GSK3_1	361	368	PF00069	0.638
MOD_GSK3_1	412	419	PF00069	0.625
MOD_GSK3_1	481	488	PF00069	0.587
MOD_GSK3_1	509	516	PF00069	0.772
MOD_GSK3_1	519	526	PF00069	0.821
MOD_N-GLC_1	117	122	PF02516	0.673
MOD_N-GLC_1	159	164	PF02516	0.560
MOD_N-GLC_1	173	178	PF02516	0.478
MOD_N-GLC_1	399	404	PF02516	0.642
MOD_N-GLC_1	457	462	PF02516	0.676
MOD_NEK2_1	194	199	PF00069	0.543
MOD_NEK2_1	234	239	PF00069	0.587
MOD_NEK2_1	407	412	PF00069	0.608
MOD_NEK2_1	440	445	PF00069	0.618
MOD_NEK2_1	491	496	PF00069	0.651
MOD_NEK2_1	518	523	PF00069	0.782
MOD_NEK2_1	741	746	PF00069	0.731
MOD_NEK2_1	80	85	PF00069	0.618
MOD_NEK2_1	86	91	PF00069	0.562
MOD_NEK2_2	173	178	PF00069	0.580
MOD_NEK2_2	300	305	PF00069	0.539
MOD_PIKK_1	57	63	PF00454	0.510
MOD_PIKK_1	598	604	PF00454	0.710
MOD_PIKK_1	80	86	PF00454	0.607
MOD_PK_1	553	559	PF00069	0.721
MOD_PKA_1	167	173	PF00069	0.608
MOD_PKA_2	11	17	PF00069	0.650
MOD_PKA_2	491	497	PF00069	0.701
MOD_PKA_2	523	529	PF00069	0.810
MOD_PKA_2	552	558	PF00069	0.608
MOD_PKA_2	80	86	PF00069	0.611
MOD_PKB_1	551	559	PF00069	0.721
MOD_Plk_1	159	165	PF00069	0.563
MOD_Plk_1	173	179	PF00069	0.482
MOD_Plk_1	474	480	PF00069	0.652
MOD_Plk_1	526	532	PF00069	0.839
MOD_Plk_1	638	644	PF00069	0.636
MOD_Plk_1	652	658	PF00069	0.481
MOD_Plk_1	741	747	PF00069	0.672
MOD_Plk_1	86	92	PF00069	0.585
MOD_Plk_2-3	476	482	PF00069	0.678
MOD_Plk_4	167	173	PF00069	0.593
MOD_Plk_4	300	306	PF00069	0.544
MOD_Plk_4	368	374	PF00069	0.604
MOD_Plk_4	498	504	PF00069	0.722
MOD_Plk_4	553	559	PF00069	0.615
MOD_Plk_4	638	644	PF00069	0.600
MOD_Plk_4	93	99	PF00069	0.618
MOD_ProDKin_1	361	367	PF00069	0.696
MOD_ProDKin_1	383	389	PF00069	0.643
MOD_ProDKin_1	41	47	PF00069	0.675
MOD_SUMO_for_1	596	599	PF00179	0.740
MOD_SUMO_rev_2	20	30	PF00179	0.618
MOD_SUMO_rev_2	570	578	PF00179	0.724
MOD_SUMO_rev_2	667	673	PF00179	0.710
TRG_DiLeu_BaEn_1	49	54	PF01217	0.589
TRG_DiLeu_BaEn_1	65	70	PF01217	0.487
TRG_DiLeu_BaEn_1	653	658	PF01217	0.560
TRG_DiLeu_BaEn_1	759	764	PF01217	0.628
TRG_DiLeu_BaEn_2	229	235	PF01217	0.630
TRG_DiLeu_BaEn_4	766	772	PF01217	0.749
TRG_DiLeu_BaLyEn_6	149	154	PF01217	0.586
TRG_DiLeu_LyEn_5	653	658	PF01217	0.645
TRG_DiLeu_LyEn_5	759	764	PF01217	0.749
TRG_ENDOCYTIC_2	292	295	PF00928	0.513
TRG_ENDOCYTIC_2	446	449	PF00928	0.658
TRG_ENDOCYTIC_2	525	528	PF00928	0.815
TRG_ENDOCYTIC_2	633	636	PF00928	0.570
TRG_ER_diArg_1	150	152	PF00400	0.632
TRG_ER_diArg_1	73	75	PF00400	0.634
TRG_ER_diArg_1	745	748	PF00400	0.798
TRG_ER_diArg_1	768	770	PF00400	0.748
TRG_ER_diArg_1	99	102	PF00400	0.633
TRG_NES_CRM1_1	49	62	PF08389	0.600
TRG_NLS_MonoExtC_3	661	667	PF00514	0.689
TRG_NLS_MonoExtN_4	743	750	PF00514	0.702
TRG_Pf-PMV_PEXEL_1	607	612	PF00026	0.633
TRG_Pf-PMV_PEXEL_1	762	766	PF00026	0.744
TRG_Pf-PMV_PEXEL_1	769	774	PF00026	0.768

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IGW0	Leptomonas seymouri	75%	100%
A0A0S4IU09	Bodo saltans	23%	95%
A0A1X0NTC2	Trypanosomatidae	34%	100%
A0A3Q8IDU4	Leishmania donovani	99%	100%
A0A422NFC9	Trypanosoma rangeli	32%	100%
A4H8U7	Leishmania braziliensis	92%	100%
A4HX65	Leishmania infantum	98%	100%
C9ZTS8	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	31%	100%
E9ARC2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	97%	100%
V5D8P7	Trypanosoma cruzi	32%	100%