VASt domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

hypothetical protein, conserved

Species:

Leishmania major

UniProt:

Q4QEC6_LEIMA

TriTrypDb:

LmjF.17.0740 , LMJLV39_170013000 , LMJSD75_170012600

Length:

352

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005789	endoplasmic reticulum membrane	4	2
GO:0005886	plasma membrane	3	2
GO:0016020	membrane	2	10
GO:0031090	organelle membrane	3	2
GO:0044232	organelle membrane contact site	2	2
GO:0110165	cellular anatomical entity	1	10
GO:0140268	endoplasmic reticulum-plasma membrane contact site	3	2

Expansion

Sequence features

Q4QEC6

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QEC6

Function

Biological processes
Term	Name	Level	Count
GO:0006810	transport	3	2
GO:0006869	lipid transport	5	2
GO:0009987	cellular process	1	2
GO:0015850	organic hydroxy compound transport	5	2
GO:0015918	sterol transport	6	2
GO:0032365	intracellular lipid transport	4	2
GO:0032366	intracellular sterol transport	5	2
GO:0046907	intracellular transport	3	2
GO:0051179	localization	1	2
GO:0051234	establishment of localization	2	2
GO:0051641	cellular localization	2	2
GO:0051649	establishment of localization in cell	3	2
GO:0071702	organic substance transport	4	2

Molecular functions
Term	Name	Level	Count
GO:0005215	transporter activity	1	2
GO:0005319	lipid transporter activity	2	2
GO:0005488	binding	1	2
GO:0005496	steroid binding	3	2
GO:0008289	lipid binding	2	2
GO:0015248	sterol transporter activity	3	2
GO:0032934	sterol binding	4	2
GO:0097159	organic cyclic compound binding	2	2
GO:0120013	lipid transfer activity	3	2
GO:0120015	sterol transfer activity	4	2

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	201	205	PF00656	0.607
CLV_MEL_PAP_1	285	291	PF00089	0.564
CLV_NRD_NRD_1	258	260	PF00675	0.548
CLV_NRD_NRD_1	78	80	PF00675	0.270
CLV_PCSK_KEX2_1	258	260	PF00082	0.483
CLV_PCSK_SKI1_1	102	106	PF00082	0.305
CLV_PCSK_SKI1_1	125	129	PF00082	0.270
CLV_PCSK_SKI1_1	325	329	PF00082	0.508
CLV_PCSK_SKI1_1	55	59	PF00082	0.310
DEG_APCC_DBOX_1	124	132	PF00400	0.582
DOC_CDC14_PxL_1	131	139	PF14671	0.421
DOC_CKS1_1	45	50	PF01111	0.625
DOC_CYCLIN_yCln2_LP_2	45	51	PF00134	0.504
DOC_MAPK_DCC_7	130	139	PF00069	0.421
DOC_MAPK_DCC_7	43	51	PF00069	0.531
DOC_MAPK_gen_1	299	308	PF00069	0.401
DOC_MAPK_MEF2A_6	130	139	PF00069	0.508
DOC_USP7_MATH_1	17	21	PF00917	0.594
DOC_USP7_MATH_1	218	222	PF00917	0.725
DOC_WW_Pin1_4	241	246	PF00397	0.806
DOC_WW_Pin1_4	44	49	PF00397	0.621
LIG_14-3-3_CanoR_1	102	110	PF00244	0.495
LIG_14-3-3_CanoR_1	192	202	PF00244	0.511
LIG_14-3-3_CanoR_1	251	257	PF00244	0.587
LIG_14-3-3_CanoR_1	288	294	PF00244	0.507
LIG_Actin_WH2_2	264	280	PF00022	0.474
LIG_AP2alpha_1	150	154	PF02296	0.495
LIG_BIR_II_1	1	5	PF00653	0.618
LIG_CaM_NSCaTE_8	317	324	PF13499	0.497
LIG_deltaCOP1_diTrp_1	83	89	PF00928	0.520
LIG_EH1_1	153	161	PF00400	0.475
LIG_eIF4E_1	123	129	PF01652	0.582
LIG_FHA_1	105	111	PF00498	0.481
LIG_FHA_1	134	140	PF00498	0.473
LIG_FHA_1	263	269	PF00498	0.470
LIG_FHA_1	281	287	PF00498	0.255
LIG_FHA_1	67	73	PF00498	0.561
LIG_FHA_2	117	123	PF00498	0.552
LIG_FHA_2	234	240	PF00498	0.745
LIG_FHA_2	4	10	PF00498	0.672
LIG_LIR_Gen_1	22	31	PF02991	0.555
LIG_LIR_Gen_1	279	289	PF02991	0.598
LIG_LIR_Gen_1	36	46	PF02991	0.539
LIG_LIR_Nem_3	119	123	PF02991	0.470
LIG_LIR_Nem_3	22	27	PF02991	0.571
LIG_LIR_Nem_3	279	284	PF02991	0.558
LIG_LIR_Nem_3	316	320	PF02991	0.501
LIG_LIR_Nem_3	337	342	PF02991	0.473
LIG_LIR_Nem_3	343	347	PF02991	0.658
LIG_LIR_Nem_3	36	41	PF02991	0.537
LIG_MYND_1	245	249	PF01753	0.759
LIG_PDZ_Class_1	347	352	PF00595	0.665
LIG_Pex14_2	150	154	PF04695	0.495
LIG_Pex14_2	61	65	PF04695	0.481
LIG_SH2_STAT5	118	121	PF00017	0.561
LIG_SH2_STAT5	180	183	PF00017	0.561
LIG_SH2_STAT5	332	335	PF00017	0.458
LIG_SH3_1	242	248	PF00018	0.734
LIG_SH3_1	85	91	PF00018	0.497
LIG_SH3_2	88	93	PF14604	0.528
LIG_SH3_3	242	248	PF00018	0.744
LIG_SH3_3	85	91	PF00018	0.497
LIG_SUMO_SIM_anti_2	304	310	PF11976	0.401
LIG_SUMO_SIM_par_1	282	287	PF11976	0.457
LIG_SUMO_SIM_par_1	332	337	PF11976	0.418
LIG_WRC_WIRS_1	159	164	PF05994	0.528
MOD_CDK_SPxK_1	44	50	PF00069	0.625
MOD_CK1_1	167	173	PF00069	0.563
MOD_CK1_1	233	239	PF00069	0.738
MOD_CK1_1	33	39	PF00069	0.547
MOD_CK2_1	116	122	PF00069	0.552
MOD_CK2_1	22	28	PF00069	0.433
MOD_CK2_1	233	239	PF00069	0.695
MOD_CK2_1	287	293	PF00069	0.495
MOD_CK2_1	3	9	PF00069	0.638
MOD_CMANNOS	86	89	PF00535	0.281
MOD_GlcNHglycan	131	134	PF01048	0.327
MOD_GlcNHglycan	166	169	PF01048	0.361
MOD_GlcNHglycan	220	223	PF01048	0.528
MOD_GlcNHglycan	226	229	PF01048	0.551
MOD_GlcNHglycan	289	292	PF01048	0.683
MOD_GlcNHglycan	32	35	PF01048	0.430
MOD_GlcNHglycan	321	324	PF01048	0.680
MOD_GSK3_1	129	136	PF00069	0.589
MOD_GSK3_1	160	167	PF00069	0.555
MOD_GSK3_1	193	200	PF00069	0.520
MOD_GSK3_1	22	29	PF00069	0.564
MOD_GSK3_1	233	240	PF00069	0.654
MOD_GSK3_1	276	283	PF00069	0.370
MOD_NEK2_1	104	109	PF00069	0.501
MOD_NEK2_1	129	134	PF00069	0.582
MOD_NEK2_1	183	188	PF00069	0.547
MOD_NEK2_1	284	289	PF00069	0.390
MOD_NEK2_1	3	8	PF00069	0.617
MOD_NEK2_1	334	339	PF00069	0.385
MOD_NEK2_2	171	176	PF00069	0.508
MOD_NEK2_2	340	345	PF00069	0.399
MOD_PIKK_1	140	146	PF00454	0.561
MOD_PIKK_1	160	166	PF00454	0.350
MOD_PIKK_1	22	28	PF00454	0.644
MOD_PKA_1	79	85	PF00069	0.481
MOD_PKA_2	287	293	PF00069	0.508
MOD_PKA_2	89	95	PF00069	0.521
MOD_Plk_1	171	177	PF00069	0.565
MOD_Plk_1	36	42	PF00069	0.539
MOD_Plk_4	105	111	PF00069	0.481
MOD_Plk_4	116	122	PF00069	0.478
MOD_Plk_4	183	189	PF00069	0.528
MOD_Plk_4	276	282	PF00069	0.356
MOD_Plk_4	309	315	PF00069	0.346
MOD_ProDKin_1	241	247	PF00069	0.803
MOD_ProDKin_1	44	50	PF00069	0.625
MOD_SUMO_rev_2	73	82	PF00179	0.512
MOD_SUMO_rev_2	8	16	PF00179	0.668
TRG_ENDOCYTIC_2	118	121	PF00928	0.510
TRG_ENDOCYTIC_2	126	129	PF00928	0.443
TRG_ENDOCYTIC_2	180	183	PF00928	0.561
TRG_ENDOCYTIC_2	332	335	PF00928	0.431
TRG_ER_diArg_1	258	260	PF00400	0.682

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0S4JTD3	Bodo saltans	26%	82%
A0A1X0NTC3	Trypanosomatidae	41%	99%
A0A3Q8IAW1	Leishmania donovani	93%	100%
A0A3R7N4K3	Trypanosoma rangeli	45%	100%
A0A3R7RDH3	Trypanosoma rangeli	45%	100%
A4H910	Leishmania braziliensis	79%	98%
A4HXE2	Leishmania infantum	93%	100%
E9AR37	Leishmania mexicana (strain MHOM/GT/2001/U1103)	90%	100%
V5BE10	Trypanosoma cruzi	43%	99%